FastQCFastQC Report
Thu 26 May 2016
SRR938643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186135
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57150.48181699384977256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49150.4143710454543538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.2798163784054935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.11271904125584357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.051.894471
GTATCAA60450.040.7239761
ATCAACG80200.030.624433
TATCAAC80450.030.5986422
TCAACGC81700.030.0621724
CAACGCA82800.029.6627945
AACGCAG85400.028.8539286
TGGTATC15850.025.48522
GTACATG74500.025.0061341
ACGCAGA97650.024.9911547
CGCAGAG98000.024.8534538
GTCCTAC11450.024.4885621
TACATGG74600.024.334532
GTGGTAT17550.024.1006981
GCAGAGT103200.023.3251119
GTAAGGT5150.023.0613444
ACATGGG74050.023.0314483
TAAGGTG6000.022.1696385
TAGGACC6500.021.9260164
AGTACTT59950.021.7790512-13
Produced by FastQC (version 0.11.5)