FastQCFastQC Report
Thu 26 May 2016
SRR938642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185040
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.4907851211773442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47490.40074596638088167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.2564470397623709No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.11029163572537637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.051.2370951
GTATCAA57700.039.7699361
TACCGGC600.00393881831.6866474
TATCAAC73400.030.8880062
ATCAACG73800.030.84943
TCAACGC75250.030.3812854
CAACGCA77050.029.669035
AACGCAG79400.028.7836196
ATTGGCG856.40107E-427.945827
GTACATG72000.027.044221
GTCCTAC12500.027.0415761
TACATGG72500.026.7479022
GTAGGAC15500.026.0648253
ACATGGG73450.025.3018633
ACGCAGA90000.025.2319727
CGCAGAG90700.024.984868
TAGGACC6550.023.2207494
TCCTACA18100.023.1084942
TGGTATC15900.022.7187272
GCAGAGT96850.022.7115699
Produced by FastQC (version 0.11.5)