##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938642_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1185040 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.084363397016133 31.0 31.0 34.0 28.0 34.0 2 31.162688179301963 31.0 31.0 34.0 28.0 34.0 3 31.22417977452238 31.0 31.0 34.0 27.0 34.0 4 34.86186373455748 37.0 35.0 37.0 32.0 37.0 5 34.9051272530885 37.0 35.0 37.0 32.0 37.0 6 34.94705663943833 37.0 35.0 37.0 32.0 37.0 7 34.87863025720651 37.0 35.0 37.0 32.0 37.0 8 34.84382721258354 37.0 35.0 37.0 32.0 37.0 9 36.342264396138525 39.0 35.0 39.0 32.0 39.0 10-11 36.18755358468913 39.0 35.0 39.0 31.5 39.0 12-13 36.04371919935192 38.5 35.0 39.0 30.0 39.0 14-15 36.999646847363806 39.0 36.0 41.0 31.0 41.0 16-17 36.88737721933437 39.0 36.0 41.0 31.0 41.0 18-19 36.938030361844326 39.0 36.0 41.0 31.0 41.0 20-21 37.05850266657666 39.0 36.0 41.0 31.0 41.0 22-23 36.97312748936744 39.0 36.0 41.0 31.0 41.0 24-25 36.87083431782894 39.0 36.0 41.0 30.0 41.0 26-27 36.696258354148384 39.0 36.0 41.0 30.0 41.0 28-29 36.50958195503949 39.0 36.0 41.0 30.0 41.0 30-31 36.28563086478094 39.0 35.0 40.0 29.5 41.0 32-33 35.9881729730642 38.0 35.0 40.0 29.0 41.0 34-35 35.75568546209411 38.0 35.0 40.0 27.5 41.0 36-37 35.475808833457094 38.0 34.5 40.0 27.0 41.0 38-39 35.21375565381759 38.0 34.0 40.0 26.0 41.0 40-41 35.10444668534396 38.0 34.0 40.0 25.0 41.0 42-43 34.91703318031459 38.0 34.0 40.0 25.0 41.0 44-45 34.57722735097549 38.0 33.5 40.0 24.0 41.0 46-47 34.12854080874907 38.0 33.0 40.0 22.5 41.0 48-49 33.73008463849321 38.0 33.0 40.0 19.5 41.0 50-51 32.99026615135354 37.5 32.0 39.5 17.5 40.5 52-53 33.07062040099912 37.5 32.5 39.5 18.0 40.5 54-55 33.235626645514074 38.0 32.5 40.0 16.0 41.0 56-57 32.96754835279822 37.5 32.0 40.0 13.5 41.0 58-59 32.68129641193546 37.0 31.5 40.0 10.5 41.0 60-61 32.28432542361439 37.0 31.0 40.0 8.0 41.0 62-63 31.803383430095188 36.0 31.0 39.0 7.0 41.0 64-65 31.350045990008773 36.0 30.0 39.0 2.0 40.0 66-67 30.83865101599946 35.0 29.0 39.0 2.0 40.0 68-69 30.31403032809019 35.0 28.5 38.0 2.0 40.0 70-71 29.717310386147304 34.5 28.0 38.0 2.0 40.0 72-73 29.18960372645649 34.0 27.5 37.0 2.0 39.0 74-75 28.603498194153786 34.0 26.0 36.5 2.0 39.0 76-77 27.8851068318369 33.5 26.0 36.0 2.0 39.0 78-79 27.1273750253156 33.0 24.5 35.5 2.0 37.5 80-81 26.481067305744954 32.0 22.5 35.0 2.0 37.0 82-83 25.83566124350233 32.0 20.5 35.0 2.0 36.5 84-85 25.177612148113145 31.0 19.0 35.0 2.0 36.0 86-87 24.51051188145548 31.0 15.0 34.0 2.0 35.5 88-89 23.743399800850604 31.0 7.0 34.0 2.0 35.0 90-91 22.92277222709782 30.0 2.0 34.0 2.0 35.0 92-93 22.09356055491798 29.0 2.0 34.0 2.0 35.0 94-95 21.151423580638628 29.0 2.0 34.0 2.0 35.0 96-97 19.90293534395463 28.0 2.0 34.0 2.0 35.0 98-99 18.446936390332816 25.5 2.0 33.0 2.0 35.0 100-101 15.065831533112807 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7380873219729693 1101 2 0.5899255921814301 1101 3 0.7179505983411758 1101 4 0.46310697438079274 1101 5 0.6075093502990612 1101 6 0.6465600693131464 1101 7 0.3811591682972306 1101 8 0.45915244144500633 1101 9 0.4962440645338049 1101 10-11 0.4883073474142918 1101 12-13 0.6464745400729726 1101 14-15 0.6963008006709899 1101 16-17 0.7204263320921385 1101 18-19 0.6366255580948277 1101 20-21 0.8673122455362332 1101 22-23 0.8199844469922297 1101 24-25 0.7352021078341977 1101 26-27 0.8953671539373929 1101 28-29 1.1041340574720309 1101 30-31 0.7011249937084401 1101 32-33 0.9340993888237037 1101 34-35 0.932563706044121 1101 36-37 0.7495388463788899 1101 38-39 0.8927091918305834 1101 40-41 0.7993932832245392 1101 42-43 0.8495866527480374 1101 44-45 0.9943579030195693 1101 46-47 1.0805410763092382 1101 48-49 1.107106417947577 1101 50-51 0.8061053701046461 1101 52-53 0.7461367873691955 1101 54-55 0.7811982977031917 1101 56-57 0.7902827343639203 1101 58-59 0.7106811539776103 1101 60-61 1.0072617793014444 1101 62-63 0.7064189445440192 1101 64-65 0.61998942830256 1101 66-67 0.6095775539970063 1101 68-69 0.7208022022232718 1101 70-71 0.847443686385887 1101 72-73 0.619693125260028 1101 74-75 0.6124572826453978 1101 76-77 0.9071848738465427 1101 78-79 1.0569035948765944 1101 80-81 0.9130832251256003 1101 82-83 0.7949233474047013 1101 84-85 0.7382760480876236 1101 86-87 1.175351498295953 1101 88-89 1.0440120296697586 1101 90-91 1.0099345953031396 1101 92-93 0.972046164292621 1101 94-95 0.9358603891558133 1101 96-97 1.0630760770681853 1101 98-99 1.2310888087884813 1101 100-101 1.248407541150053 1102 1 0.3590383895723761 1102 2 0.5399612092703272 1102 3 0.47933893420119134 1102 4 0.7045753117020226 1102 5 0.5355308666737884 1102 6 0.5957993248246609 1102 7 0.45377784626394657 1102 8 0.44982533044754547 1102 9 0.6635265719768597 1102 10-11 0.6195764116894367 1102 12-13 0.7073281629701924 1102 14-15 0.7411075551322668 1102 16-17 0.7208866144717305 1102 18-19 0.7345885345875516 1102 20-21 0.6753247206499395 1102 22-23 0.7774535331855859 1102 24-25 0.7098701383005093 1102 26-27 0.521632017222565 1102 28-29 0.4296993646009284 1102 30-31 0.43668454730759265 1102 32-33 0.4836459558842918 1102 34-35 0.29737265232623855 1102 36-37 0.5780612851448907 1102 38-39 0.4792897686447688 1102 40-41 0.3462914000851214 1102 42-43 0.5164833083947116 1102 44-45 0.6098691452340432 1102 46-47 0.8216943393825318 1102 48-49 0.5331014132899554 1102 50-51 0.5375785476969313 1102 52-53 0.6460125841874031 1102 54-55 0.8746657582960822 1102 56-57 0.7917275096109577 1102 58-59 0.681312124406297 1102 60-61 0.8362253220145561 1102 62-63 0.5109943705791977 1102 64-65 0.6109440576108582 1102 66-67 0.5012226068746806 1102 68-69 0.6404290447226799 1102 70-71 0.793118949861018 1102 72-73 0.6063490459161152 1102 74-75 0.4945313844554775 1102 76-77 0.5469422210126957 1102 78-79 0.5438732198925749 1102 80-81 0.7428029460337449 1102 82-83 0.8476900646050147 1102 84-85 0.7946423744132183 1102 86-87 0.7170659588340484 1102 88-89 0.9016231275893567 1102 90-91 0.7903977087846634 1102 92-93 0.7808645901999434 1102 94-95 1.148868845931279 1102 96-97 1.0831531561015915 1102 98-99 1.1830482053071414 1102 100-101 1.0030715532781311 1103 1 0.671721827727513 1103 2 0.583259376579452 1103 3 0.4906157313879085 1103 4 0.42385914209358333 1103 5 0.43819875062590796 1103 6 0.4972194911870105 1103 7 0.35816007389632887 1103 8 0.4651394577215058 1103 9 0.4767138310557826 1103 10-11 0.5340080070096462 1103 12-13 0.4874542872685623 1103 14-15 0.7070235624359711 1103 16-17 0.6148407633863187 1103 18-19 0.41194026245662485 1103 20-21 0.5434352231183226 1103 22-23 0.6533884760407958 1103 24-25 0.7259028021268463 1103 26-27 0.6966675213816202 1103 28-29 0.8343622441572975 1103 30-31 0.863295759859156 1103 32-33 1.002319452874886 1103 34-35 0.8825302231654888 1103 36-37 0.7989681628077108 1103 38-39 0.6548510724927397 1103 40-41 1.0231950992803576 1103 42-43 0.8268555948625576 1103 44-45 1.0329772807584945 1103 46-47 1.104192479640865 1103 48-49 1.0205718264907446 1103 50-51 1.0255899766547998 1103 52-53 0.88448049519031 1103 54-55 1.02951461201895 1103 56-57 1.281292668196194 1103 58-59 1.2628719404609328 1103 60-61 1.2128973988387912 1103 62-63 1.3300431972512712 1103 64-65 1.400179534719129 1103 66-67 1.5284323181075194 1103 68-69 1.6789121717937583 1103 70-71 1.6924426801646604 1103 72-73 1.5756531643985632 1103 74-75 1.441704577124714 1103 76-77 1.7635784555430298 1103 78-79 1.6286740584669985 1103 80-81 1.3500187436938518 1103 82-83 1.6863980513501282 1103 84-85 1.7053578677519603 1103 86-87 1.7198403552633437 1103 88-89 1.5269578810890536 1103 90-91 1.6715450003245316 1103 92-93 2.1384421921476005 1103 94-95 1.9689641999584637 1103 96-97 1.6568428835649023 1103 98-99 1.3920933124958843 1103 100-101 1.2869492837170302 1104 1 0.35433137389339464 1104 2 0.2906587909870453 1104 3 0.43457744071195137 1104 4 0.4292165553905676 1104 5 0.3482301757357078 1104 6 0.39646036654669103 1104 7 0.3753397649458492 1104 8 0.21804217870691645 1104 9 0.4079193437015505 1104 10-11 0.47436813371972875 1104 12-13 0.43012081510944 1104 14-15 0.5270911453528342 1104 16-17 0.6440131092896948 1104 18-19 0.5257458558737582 1104 20-21 0.4978483055555998 1104 22-23 0.6319130734460217 1104 24-25 0.7056231943727909 1104 26-27 0.6211951981951884 1104 28-29 0.5286978365647812 1104 30-31 0.6337962268186175 1104 32-33 0.35125491150480315 1104 34-35 0.28851834725050196 1104 36-37 0.4619338602179752 1104 38-39 0.436539635772057 1104 40-41 0.595195200244568 1104 42-43 0.7458365504887823 1104 44-45 0.5840004899058471 1104 46-47 0.3909907783939559 1104 48-49 0.5433392554371821 1104 50-51 0.5050895096953383 1104 52-53 0.4215390922926403 1104 54-55 0.49076328686371085 1104 56-57 0.9198550009742306 1104 58-59 0.6320097061230072 1104 60-61 0.676577102508837 1104 62-63 0.5762365310894282 1104 64-65 0.49389482827256614 1104 66-67 0.6866448099041804 1104 68-69 0.8467919865244333 1104 70-71 0.7516992049766174 1104 72-73 0.7378332340638671 1104 74-75 0.7545200902753031 1104 76-77 0.955427804323886 1104 78-79 0.9120269536156655 1104 80-81 0.8208590183164972 1104 82-83 0.7938334342568858 1104 84-85 0.8222965067544337 1104 86-87 0.6877857967293437 1104 88-89 0.6215739646874177 1104 90-91 0.5523662179528799 1104 92-93 0.6430423738831728 1104 94-95 0.7203792764388552 1104 96-97 0.7291237912331354 1104 98-99 0.6374994410233263 1104 100-101 0.42870934227627266 1105 1 0.3875392677123308 1105 2 0.6034692575438569 1105 3 0.5654973538715247 1105 4 0.488535964586994 1105 5 0.5792646533099486 1105 6 0.40155637814144995 1105 7 0.502412298352958 1105 8 0.26067156733586927 1105 9 0.5542851559963253 1105 10-11 0.5464418548681351 1105 12-13 0.5724374068030684 1105 14-15 0.7835744838981924 1105 16-17 0.8088069534820832 1105 18-19 0.7722337499332497 1105 20-21 0.6508959144368944 1105 22-23 0.6518178887597585 1105 24-25 0.7589057956164638 1105 26-27 0.8008301707117553 1105 28-29 0.6613087776167319 1105 30-31 0.7026795846084042 1105 32-33 0.7263368506202781 1105 34-35 0.6873397019371197 1105 36-37 0.8839210566673827 1105 38-39 0.8529681277315078 1105 40-41 0.7496496811865825 1105 42-43 0.7643205866442244 1105 44-45 0.6219153724547724 1105 46-47 0.8024912715321193 1105 48-49 0.8365414661394794 1105 50-51 0.6894085430242569 1105 52-53 0.7382559497997576 1105 54-55 0.6682343901701415 1105 56-57 0.9057535798943732 1105 58-59 0.9050037764372973 1105 60-61 0.7191189140097833 1105 62-63 0.7270184106674975 1105 64-65 0.880445612482518 1105 66-67 0.9116567624907503 1105 68-69 0.8384608833382821 1105 70-71 0.7882944167253321 1105 72-73 0.7227714715159088 1105 74-75 0.7944890887237186 1105 76-77 0.7096393996859831 1105 78-79 0.7663294337397915 1105 80-81 0.47429715353257507 1105 82-83 0.543894324792241 1105 84-85 0.7777885323495077 1105 86-87 0.701255008670504 1105 88-89 1.0544605669654743 1105 90-91 1.037202971179326 1105 92-93 1.0969144105437856 1105 94-95 1.0445796559146814 1105 96-97 1.1472831530401884 1105 98-99 1.162395400898717 1105 100-101 0.8184253067222134 1106 1 0.26788652033890514 1106 2 0.5005173501252926 1106 3 0.18995892590058006 1106 4 0.3760451581407409 1106 5 0.390166437979218 1106 6 0.010564832598504381 1106 7 0.1304978283857139 1106 8 0.372822808339194 1106 9 0.31800251617121944 1106 10-11 0.1492127811304229 1106 12-13 0.1453377725222751 1106 14-15 0.39413807583590454 1106 16-17 0.04898737110423923 1106 18-19 -0.13595786901363738 1106 20-21 0.07584111435424035 1106 22-23 0.24669897583157052 1106 24-25 0.3798586451485093 1106 26-27 0.08936579112671694 1106 28-29 0.12017042420568913 1106 30-31 0.06515883138000333 1106 32-33 0.4075557027156904 1106 34-35 0.14151353921447196 1106 36-37 -0.03734672175099263 1106 38-39 0.1371641740337779 1106 40-41 0.1952881436920606 1106 42-43 -0.18902781761493515 1106 44-45 0.25926814916086727 1106 46-47 0.005503361506846716 1106 48-49 0.5077973236433664 1106 50-51 0.21458984929638802 1106 52-53 0.5552294535805018 1106 54-55 0.3430755530156233 1106 56-57 -0.09570185539239873 1106 58-59 -0.4629117685796942 1106 60-61 0.42959999876965327 1106 62-63 0.6156012090046374 1106 64-65 0.7136904420567944 1106 66-67 0.2291150501272412 1106 68-69 0.08534013242181615 1106 70-71 0.08346709659025464 1106 72-73 0.49682968314754206 1106 74-75 0.4661824076652614 1106 76-77 0.4730580145190686 1106 78-79 0.6776024284613555 1106 80-81 0.5254299294006017 1106 82-83 0.6872895601833413 1106 84-85 0.7483185867811244 1106 86-87 0.8298097892462053 1106 88-89 0.6097385699160824 1106 90-91 0.5322240025623977 1106 92-93 0.5839964741191075 1106 94-95 0.6608887673947414 1106 96-97 0.620604868900493 1106 98-99 0.542455829097964 1106 100-101 0.028852927986809718 1107 1 0.5956280916498145 1107 2 0.5176359304880442 1107 3 0.4705518073609234 1107 4 0.5074705621899867 1107 5 0.3690823335906188 1107 6 0.4619520536379653 1107 7 0.41275561933441196 1107 8 0.3240797675115914 1107 9 0.48526553794802396 1107 10-11 0.638744679683569 1107 12-13 0.4484150734421206 1107 14-15 0.7315695696274318 1107 16-17 0.5974915985088884 1107 18-19 0.5652519380766776 1107 20-21 0.5656861163013858 1107 22-23 0.39938352365327745 1107 24-25 0.5535720039100482 1107 26-27 0.7037048410563713 1107 28-29 0.5441154726943083 1107 30-31 0.6202443360836725 1107 32-33 0.6298326530139988 1107 34-35 0.6867815733593616 1107 36-37 0.667268211957925 1107 38-39 0.901513672050676 1107 40-41 0.6943966640393668 1107 42-43 0.5892681807676539 1107 44-45 0.5988180944101842 1107 46-47 0.7292596659776791 1107 48-49 0.8770937874407494 1107 50-51 0.7403735576075121 1107 52-53 0.7330335098807623 1107 54-55 0.8266751090397548 1107 56-57 0.7496885027870306 1107 58-59 0.7410036860410116 1107 60-61 0.6177072938412778 1107 62-63 0.7628622497155391 1107 64-65 0.9025386014973193 1107 66-67 0.7035145225666852 1107 68-69 0.7946580622893684 1107 70-71 0.7913528243673795 1107 72-73 0.7179743050285481 1107 74-75 0.8562776887934369 1107 76-77 0.8220488921176887 1107 78-79 1.0833430418047882 1107 80-81 0.8564768955881483 1107 82-83 1.0386075117612101 1107 84-85 0.891818994527906 1107 86-87 0.6739409493773891 1107 88-89 0.7715804591473621 1107 90-91 0.8658629763702628 1107 92-93 0.7848969092751581 1107 94-95 0.7820791166186645 1107 96-97 0.7695355682303209 1107 98-99 0.554397178692323 1107 100-101 0.4792574182219216 1108 1 0.33642192605593024 1108 2 0.3710682388245097 1108 3 0.42296664561150266 1108 4 0.40491001623609435 1108 5 0.45046961802095353 1108 6 0.43198536286409706 1108 7 0.36634786098791494 1108 8 0.27965198233965083 1108 9 0.17590456799987209 1108 10-11 0.19101268121386283 1108 12-13 0.3387368819853549 1108 14-15 0.5784551924673806 1108 16-17 0.5066650871261444 1108 18-19 0.4097866250260864 1108 20-21 0.4680564712836812 1108 22-23 0.3158462683515708 1108 24-25 0.3242222080040591 1108 26-27 0.46177037694265977 1108 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2316 58-59 -0.9987007559111873 2316 60-61 -1.1063684954330526 2316 62-63 -1.214035219339344 2316 64-65 -1.0486552600804302 2316 66-67 -1.069599992860752 2316 68-69 -0.9446471928321465 2316 70-71 -1.2594066367156032 2316 72-73 -1.0803233066564708 2316 74-75 -0.940651028689409 2316 76-77 -0.9574597145160908 2316 78-79 -0.9303870321439085 2316 80-81 -0.647120816411423 2316 82-83 -0.5315674024979593 2316 84-85 -0.6064019756529255 2316 86-87 -0.6954157650397974 2316 88-89 -0.841929751335659 2316 90-91 -0.9003928642154051 2316 92-93 -0.9043265258621638 2316 94-95 -0.7963236625975796 2316 96-97 -0.8752399372633057 2316 98-99 -1.014687295632772 2316 100-101 -1.2562823421476157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3585.0 3 1265.0 4 1252.0 5 1457.0 6 1673.0 7 2054.0 8 2629.0 9 3417.0 10 4418.0 11 5651.0 12 7134.0 13 9014.0 14 10382.0 15 12759.0 16 15206.0 17 18559.0 18 16243.0 19 13098.0 20 13438.0 21 14359.0 22 16117.0 23 17673.0 24 19710.0 25 22058.0 26 25148.0 27 28631.0 28 32782.0 29 37702.0 30 44754.0 31 51625.0 32 61081.0 33 74610.0 34 91326.0 35 114712.0 36 141102.0 37 155623.0 38 87380.0 39 5413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83852098464629 19.301918009267517 14.822279579419382 22.037281426666812 2 14.735682354480714 20.534840624248076 42.15996251472184 22.56951450654937 3 19.1512767151969 25.84861775370731 29.781212932202607 25.218892598893188 4 12.223294470659653 17.082859475510098 37.955906104808484 32.737939949021765 5 12.907444761445605 38.48178303442441 34.19922777053994 14.41154443359004 6 30.476883664298487 36.84749145919072 18.360080181438367 14.315544695072422 7 28.031571838595305 33.532416005402666 20.824160054026674 17.61185210197535 8 25.982227347127246 35.8411789682677 20.102918297627358 18.073675386977694 9 27.06881676495864 16.74174338956208 20.62299610152579 35.56644374395348 10-11 24.56462185902604 27.33467280685833 27.656230873457744 20.44447446065789 12-13 26.08217036398271 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30.751404387666714 21.047452853604298 38-39 20.87706029868686 27.725772947002625 30.581555626270873 20.815611128039645 40-41 21.46433878502405 27.149174987412845 30.808544832007374 20.577941395555733 42-43 20.69189041368757 27.656119306235826 30.784666896807263 20.86732338326934 44-45 20.718954248366014 27.982885859314678 30.37726215943662 20.920897732882697 46-47 21.186394904587612 27.810279101601893 29.727135163966178 21.276190829844317 48-49 21.181959197038772 27.98798056230241 29.449901005473816 21.380159235185 50-51 21.171597128774327 28.49595693245885 29.20152633553304 21.130919603233778 52-53 21.21803519488504 28.26456547782059 29.349536444129185 21.167862883165185 54-55 20.965162431565897 28.095474804181624 29.576698098318925 21.362664665933554 56-57 21.059576213398866 28.37423286996091 29.025027512034406 21.54116340460582 58-59 21.18400608996026 28.103233941063433 29.014407110805035 21.698352858171273 60-61 20.934920411206654 27.870593626651296 29.721842209868655 21.47264375227339 62-63 21.085392209729864 27.892026184165115 29.649381303317917 21.37320030278711 64-65 20.93598002616987 28.05252202518164 29.27881797594077 21.73267997270772 66-67 20.829658909377525 28.399281683520496 29.034601892163074 21.736457514938905 68-69 21.32541055928219 27.827351377307767 29.43549390959953 21.411744153810513 70-71 21.267184683011813 27.893373937351875 29.097399002005442 21.742042377630877 72-73 21.224923520879678 28.065948655490946 28.96479196286632 21.744335860763055 74-75 20.924505927185717 28.301731523174894 29.238307048898754 21.535455500740635 76-77 21.087027340888454 27.755694189850217 29.412427461743352 21.744851007517983 78-79 21.10242559623017 27.944736946481267 29.111416412163173 21.84142104512539 80-81 21.176834460368937 28.220110771017254 28.92283076237566 21.68022400623815 82-83 20.998026967589272 28.248414738501477 29.127460594205125 21.62609769970413 84-85 21.081577386151416 27.924096160840794 29.101464759112655 21.892861693895135 86-87 20.99046532392412 28.216708384742496 28.758762061812515 22.03406422952087 88-89 21.255591110946042 27.82843318902028 28.90423932613766 22.01173637389602 90-91 21.2140256835041 27.987675294663866 28.94575230296827 21.852546718863763 92-93 21.23911283822051 27.900886841041316 29.00034352746152 21.859656793276653 94-95 21.12160545777308 27.84683187313818 28.89924442392283 22.13231824516591 96-97 21.58530027747271 27.493096948306672 28.68816271195598 22.233440062264638 98-99 21.482969805313736 27.382851470908477 28.676595952997104 22.457582770780686 100-101 21.909952640670223 27.031358711332825 28.932448639142688 22.126240008854264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 470.0 1 323.0 2 234.0 3 290.5 4 255.5 5 261.0 6 452.5 7 932.5 8 1501.5 9 1731.0 10 1521.5 11 1301.5 12 1339.0 13 1483.5 14 1633.5 15 1782.0 16 1993.5 17 2256.5 18 2419.5 19 2593.5 20 2905.0 21 3329.5 22 3903.5 23 4574.0 24 5311.0 25 6342.0 26 7872.5 27 9871.5 28 11825.5 29 14012.5 30 16836.5 31 20045.0 32 23931.5 33 27576.5 34 31246.0 35 35232.0 36 38440.0 37 40555.0 38 41145.0 39 40727.5 40 40758.0 41 41478.5 42 42158.5 43 42835.5 44 43243.5 45 43631.5 46 43150.5 47 42619.5 48 42950.5 49 42310.5 50 40709.0 51 38123.5 52 35106.0 53 32945.5 54 31270.0 55 28570.5 56 24978.5 57 21807.5 58 19357.0 59 16672.0 60 13498.5 61 10829.0 62 8949.0 63 6844.5 64 4952.5 65 3814.5 66 2810.0 67 2066.0 68 1552.0 69 1180.5 70 864.5 71 641.0 72 528.5 73 443.0 74 362.5 75 285.0 76 177.5 77 111.5 78 83.5 79 52.0 80 31.0 81 23.0 82 13.5 83 5.0 84 8.0 85 5.5 86 2.5 87 2.0 88 1.5 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18556335651117262 2 0.048521568892189296 3 0.046833862148113144 4 0.051728211705933974 5 0.057128873286977654 6 0.061263754809964224 7 0.03712954836967528 8 0.03468237359076487 9 0.03999864983460474 10-11 0.041770741915884695 12-13 0.03919698913116857 14-15 0.034766758927968676 16-17 0.03295247417808682 18-19 0.04046276918922568 20-21 0.04885911024100452 22-23 0.046285357456288395 24-25 0.0654830216701546 26-27 0.049745156281644505 28-29 0.042952136636738 30-31 0.05109532167690542 32-33 0.04881691757240262 34-35 0.04700263282252076 36-37 0.045399311415648416 38-39 0.027087693242422196 40-41 0.02535779382974414 42-43 0.021687031661378517 44-45 0.00464119354620941 46-47 0.011054479173698778 48-49 0.01063255248767974 50-51 0.009324579761020725 52-53 0.010421589144670222 54-55 0.011898332545736853 56-57 0.008776075069195977 58-59 0.011729561871329237 60-61 0.011560791196921622 62-63 0.004134881522986566 64-65 0.007552487679740769 66-67 0.003966110848578951 68-69 0.008565111726186458 70-71 0.022277729021805172 72-73 0.02021028826031189 74-75 0.02067440761493283 76-77 0.012489029906163506 78-79 0.004472422871801796 80-81 0.006160129615877944 82-83 0.0052318909066360626 84-85 0.0056538175926551006 86-87 0.008607304394788361 88-89 0.02928171200972119 90-91 0.03607473165462769 92-93 0.02303719705663944 94-95 0.032150813474650645 96-97 0.035357456288395324 98-99 0.022108958347397557 100-101 0.12062883953284279 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1185040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.2005203345939 #Duplication Level Percentage of deduplicated Percentage of total 1 85.12682558732885 59.75947450662707 2 9.56126327041626 13.424113132785251 3 2.421389298132311 5.099483659845161 4 1.014042059705785 2.8474512093003788 5 0.49485992005653984 1.7369711940352324 6 0.3141295159807351 1.323123328458104 7 0.19768684682998036 0.9714403657538851 8 0.1316782133740452 0.7395103276470125 9 0.11261449934345541 0.711503680601732 >10 0.5665758004242387 7.247463950096926 >50 0.03674212655928525 1.7319147091547544 >100 0.02074550685031852 2.7431741529077662 >500 9.649033321670818E-4 0.40184806761490277 >1k 3.618387495626557E-4 0.7700817899194145 >5k 1.2061291652088523E-4 0.492445925252481 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5816 0.4907851211773442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4749 0.40074596638088167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3039 0.2564470397623709 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1307 0.11029163572537637 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.531560116114224E-4 0.0 0.0 0.0 0.0 8 3.3754134881522986E-4 0.0 0.0 0.0 0.0 9 3.3754134881522986E-4 0.0 0.0 0.0 0.0 10-11 3.797340174171336E-4 0.0 0.0 0.0 0.0 12-13 4.2192668601903735E-4 0.0 0.0 0.0 0.0 14-15 5.063120232228448E-4 0.0 0.0 4.219266860190373E-5 0.0 16-17 5.063120232228448E-4 0.0 0.0 8.438533720380746E-5 0.0 18-19 7.594680348342673E-4 0.0 0.0 8.438533720380746E-5 0.0 20-21 0.0010126240464456897 0.0 0.0 2.1096334300951868E-4 0.0 22-23 0.0010126240464456897 0.0 0.0 4.2192668601903735E-4 0.0 24-25 0.0013501653952609194 0.0 0.0 0.001097009383649497 0.0 26-27 0.0013501653952609194 0.0 0.0 0.0019830554242894756 0.0 28-29 0.0021518260986970903 0.0 0.0 0.004767771552015122 0.0 30-31 0.002362789441706609 0.0 0.0 0.01561128738270438 0.0 32-33 0.002362789441706609 0.0 0.0 0.03451360291635725 0.0 34-35 0.0025737527847161275 0.0 0.0 0.06531425099574698 0.0 36-37 0.0028691014649294536 0.0 0.0 0.12176804158509417 0.0 38-39 0.0032066428137446836 0.0 0.0 0.20902248025383108 0.0 40-41 0.0032066428137446836 0.0 0.0 0.3012978464861945 0.0 42-43 0.0032488354823465874 0.0 0.0 0.38775062445149533 0.0 44-45 0.003459798825356106 0.0 0.0 0.49576385607236884 0.0 46-47 0.0036707621683656246 0.0 0.0 0.6263079727266591 0.0 48-49 0.0037129548369675284 0.0 0.0 0.7687504219266861 0.0 50-51 0.0037129548369675284 0.0 0.0 0.9254539931141564 0.0 52-53 0.0037129548369675284 0.0 0.0 1.0762083980287587 0.0 54-55 0.0037129548369675284 0.0 0.0 1.2387345574832918 0.0 56-57 0.0038817255113751436 0.0 0.0 1.4319769796800108 0.0 58-59 0.0038817255113751436 0.0 0.0 1.6476659015729427 0.0 60-61 0.003966110848578951 0.0 0.0 1.886898332545737 0.0 62-63 0.003966110848578951 0.0 0.0 2.1166374130831027 0.0 64-65 0.003966110848578951 0.0 0.0 2.359118679538243 0.0 66-67 0.004303652197394181 0.0 0.0 2.6347211908458785 0.0 68-69 0.004388037534597988 0.0 0.0 2.9401961115236617 0.0 70-71 0.004599000877607506 0.0 0.0 3.2736447714845065 0.0 72-73 0.004852156889218929 0.0 0.0 3.609203064875447 0.0 74-75 0.004936542226422737 0.0 0.0 3.950204212516033 0.0 76-77 0.004978734895024641 0.0 0.0 4.30694322554513 0.0 78-79 0.004978734895024641 0.0 0.0 4.714018092216296 0.0 80-81 0.005611624924053197 0.0 0.0 5.149615202862351 0.0 82-83 0.0056538175926551006 0.0 0.0 5.605760143117532 0.0 84-85 0.005780395598460811 0.0 0.0 6.056124687774252 0.0 86-87 0.0059069736042665225 0.0 0.0 6.508050361169243 0.0 88-89 0.0059069736042665225 0.0 0.0 6.9906922973064205 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3280 0.0 51.237095 1 GTATCAA 5770 0.0 39.769936 1 TACCGGC 60 0.003938818 31.686647 4 TATCAAC 7340 0.0 30.888006 2 ATCAACG 7380 0.0 30.8494 3 TCAACGC 7525 0.0 30.381285 4 CAACGCA 7705 0.0 29.66903 5 AACGCAG 7940 0.0 28.783619 6 ATTGGCG 85 6.40107E-4 27.94582 7 GTACATG 7200 0.0 27.04422 1 GTCCTAC 1250 0.0 27.041576 1 TACATGG 7250 0.0 26.747902 2 GTAGGAC 1550 0.0 26.064825 3 ACATGGG 7345 0.0 25.301863 3 ACGCAGA 9000 0.0 25.231972 7 CGCAGAG 9070 0.0 24.98486 8 TAGGACC 655 0.0 23.220749 4 TCCTACA 1810 0.0 23.108494 2 TGGTATC 1590 0.0 22.718727 2 GCAGAGT 9685 0.0 22.711569 9 >>END_MODULE