FastQCFastQC Report
Thu 26 May 2016
SRR938642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185040
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57420.48454060622426254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49810.420323364612165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33620.28370350367920066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.11670492135286573No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.053.022311
GTATCAA57150.041.367611
TATCAAC75400.031.1656272
ATCAACG75600.031.1303143
TCAACGC77350.030.3030744
GGACCGA1601.9081199E-929.6839526
CAACGCA79200.029.595245
AACGCAG81350.028.782676
GTACATG71750.025.9886931
ACGCAGA93050.025.112537
GTCCTAC11800.024.9936661
TACATGG73300.024.8550762
CGCAGAG93850.024.797258
GTAGGAC14000.024.7910483
ACATGGG73200.023.4474813
TGTAGGA19250.022.7340772
GCAGAGT101250.022.5627379
AGGACCG2755.0931703E-1122.4755735
TGGTATC14350.022.2097242
GAGTACT56150.021.86515212-13
Produced by FastQC (version 0.11.5)