##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938642_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1185040 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785576014311754 31.0 31.0 34.0 30.0 34.0 2 32.00483021670155 31.0 31.0 34.0 30.0 34.0 3 32.060694153783835 33.0 31.0 34.0 30.0 34.0 4 35.73362080604874 37.0 35.0 37.0 33.0 37.0 5 35.612519408627556 37.0 35.0 37.0 33.0 37.0 6 35.65193579963545 37.0 35.0 37.0 33.0 37.0 7 35.59317153851347 37.0 35.0 37.0 33.0 37.0 8 35.58799281036927 37.0 35.0 37.0 33.0 37.0 9 37.150504624316476 39.0 37.0 39.0 33.0 39.0 10-11 37.062278910416524 39.0 37.0 39.0 33.0 39.0 12-13 36.957314099102135 39.0 37.0 39.0 33.0 39.0 14-15 37.97524176399109 40.0 37.0 41.0 33.0 41.0 16-17 37.92133683251198 40.0 37.0 41.0 33.0 41.0 18-19 38.07310597110646 40.0 37.0 41.0 33.0 41.0 20-21 38.154885911024095 40.0 37.0 41.0 33.0 41.0 22-23 38.055946634712754 40.0 37.0 41.0 33.0 41.0 24-25 37.957446162154866 40.0 37.0 41.0 33.0 41.0 26-27 37.77603583001418 40.0 37.0 41.0 32.0 41.0 28-29 37.6567238236684 40.0 37.0 41.0 32.0 41.0 30-31 37.40936761628299 39.0 36.0 41.0 32.0 41.0 32-33 37.168716667791806 39.0 36.0 41.0 31.0 41.0 34-35 37.00869801863228 39.0 35.5 41.0 31.0 41.0 36-37 36.97803407479916 39.0 36.0 41.0 31.0 41.0 38-39 36.84865321001823 39.0 35.0 41.0 31.0 41.0 40-41 36.65342562276379 39.0 35.0 41.0 30.0 41.0 42-43 36.42649699588199 39.0 35.0 40.5 30.0 41.0 44-45 36.11910399648957 39.0 35.0 40.0 30.0 41.0 46-47 35.66022708094242 38.5 35.0 40.0 28.0 41.0 48-49 35.24509383649497 38.0 34.5 40.0 26.5 41.0 50-51 34.88780589684737 38.0 34.0 40.0 25.5 41.0 52-53 34.43545112401269 38.0 33.0 40.0 24.0 41.0 54-55 34.15366780868156 38.0 33.0 40.0 23.0 41.0 56-57 33.85077043812867 38.0 33.0 40.0 23.0 41.0 58-59 33.45116620536015 37.0 32.5 40.0 22.0 41.0 60-61 33.11311770066833 37.0 32.0 39.5 20.5 41.0 62-63 32.69305339904138 36.0 31.0 39.0 18.5 40.0 64-65 32.22175158644434 36.0 31.0 39.0 17.5 40.0 66-67 31.75439521028826 35.0 30.5 38.5 13.5 40.0 68-69 31.247632569364747 35.0 30.0 38.0 10.5 40.0 70-71 30.68419336056167 34.5 29.0 37.5 8.0 39.5 72-73 30.257552909606424 34.0 29.0 37.0 7.5 39.0 74-75 29.759899665834066 34.0 28.5 36.5 6.5 39.0 76-77 28.471387463714304 32.5 27.0 35.0 2.0 37.5 78-79 29.17839144670222 34.0 28.5 36.0 2.0 38.0 80-81 28.896670576520624 34.0 28.0 35.0 2.0 37.0 82-83 28.417233173563762 33.5 27.0 35.0 2.0 37.0 84-85 27.88797804293526 33.0 26.0 35.0 2.0 36.0 86-87 27.302696533450348 32.5 26.0 35.0 2.0 36.0 88-89 26.665846300546818 32.0 25.0 34.5 2.0 35.0 90-91 26.126472524134208 32.0 24.5 34.0 2.0 35.0 92-93 25.401550158644433 31.5 21.0 34.0 2.0 35.0 94-95 24.642643708229258 31.0 18.5 34.0 2.0 35.0 96-97 23.640604114629042 31.0 4.5 34.0 2.0 35.0 98-99 22.416837828258963 30.5 2.0 34.0 2.0 35.0 100-101 18.79761611422399 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38052496485669707 1101 2 0.30100256402715075 1101 3 0.15527416887147893 1101 4 0.1513017017613194 1101 5 0.12934240499472338 1101 6 0.13156583548963852 1101 7 0.3082729006821907 1101 8 0.2966221677774854 1101 9 0.29358259069583426 1101 10-11 0.3898121149738216 1101 12-13 0.31335392156851327 1101 14-15 0.354619022580998 1101 16-17 0.4369319106759093 1101 18-19 0.5081787487055038 1101 20-21 0.45293070141513425 1101 22-23 0.4107328096891152 1101 24-25 0.43547449573159724 1101 26-27 0.33825910678188364 1101 28-29 0.4012177898113549 1101 30-31 0.37295042189021643 1101 32-33 0.21195429845933234 1101 34-35 0.2244222468497341 1101 36-37 0.48275431614444386 1101 38-39 0.5741435072539289 1101 40-41 0.4781967652667163 1101 42-43 0.6450526760453883 1101 44-45 0.43012996758204736 1101 46-47 0.4541796534263298 1101 48-49 0.7123561458306824 1101 50-51 0.7897797865320655 1101 52-53 0.706247490523225 1101 54-55 0.6294436051822956 1101 56-57 0.8002671651369084 1101 58-59 0.6861912006462276 1101 60-61 0.6991853551325491 1101 62-63 0.6968331997782258 1101 64-65 0.847127535027532 1101 66-67 0.6974846581636136 1101 68-69 0.4053617592384313 1101 70-71 0.36498739644371625 1101 72-73 0.3495236749763535 1101 74-75 0.6122249662370329 1101 76-77 0.5731475704987758 1101 78-79 0.7066852830372241 1101 80-81 0.6771356420547612 1101 82-83 0.7729970183615684 1101 84-85 0.7098342607161108 1101 86-87 0.5994009085875334 1101 88-89 0.6784926188734204 1101 90-91 0.9495223309958547 1101 92-93 0.6690859423459408 1101 94-95 0.6137774109129381 1101 96-97 0.8990504773584078 1101 98-99 0.43188780155572104 1101 100-101 0.40900021549106214 1102 1 0.42869479444262737 1102 2 0.3945796156627992 1102 3 0.38597609950034695 1102 4 0.3220372403099745 1102 5 0.26333389342374147 1102 6 0.16534179486788503 1102 7 0.24718594018975892 1102 8 0.2491619401934173 1102 9 0.40093523640625506 1102 10-11 0.42908315188773827 1102 12-13 0.36913941536749206 1102 14-15 0.4498394832286863 1102 16-17 0.353570114113424 1102 18-19 0.3902857278284131 1102 20-21 0.43253443846969475 1102 22-23 0.5095428336164929 1102 24-25 0.45545796574931785 1102 26-27 0.355216494131426 1102 28-29 0.3686249570655491 1102 30-31 0.3944037817689363 1102 32-33 0.5077391529772228 1102 34-35 0.49955329947169247 1102 36-37 0.5013724644211237 1102 38-39 0.4933534471615033 1102 40-41 0.5278154797637811 1102 42-43 0.5722422137538459 1102 44-45 0.5116387622632317 1102 46-47 0.5150172942964844 1102 48-49 0.7258627852213593 1102 50-51 0.5173369108631007 1102 52-53 0.6365667657611311 1102 54-55 0.7427703326111086 1102 56-57 0.4935843946753451 1102 58-59 0.6721461952580015 1102 60-61 0.9398892949305306 1102 62-63 0.9801785576843045 1102 64-65 0.766529715889277 1102 66-67 0.6109049946081164 1102 68-69 0.560265045143197 1102 70-71 0.6072128252186708 1102 72-73 0.5563813344769812 1102 74-75 0.6565796576469225 1102 76-77 0.6208452453421529 1102 78-79 0.5484810235986934 1102 80-81 0.48819200838108756 1102 82-83 0.3022591966661956 1102 84-85 0.2352730815164925 1102 86-87 0.30879468746933725 1102 88-89 0.5167366963908187 1102 90-91 0.4225501488555423 1102 92-93 0.331437309494202 1102 94-95 0.6543146810000628 1102 96-97 0.5761445795337892 1102 98-99 0.32057891920170434 1102 100-101 0.4407259091110376 1103 1 0.3669175576806083 1103 2 0.23552098308012148 1103 3 0.2781719472044131 1103 4 0.22133730117424477 1103 5 0.16752676554114032 1103 6 0.0624236911281173 1103 7 0.23202735494596283 1103 8 0.22787297015216978 1103 9 0.25816794080876804 1103 10-11 0.4139414733863589 1103 12-13 0.1944606434732563 1103 14-15 0.3954724716644691 1103 16-17 0.5611629321292426 1103 18-19 0.43459491833358044 1103 20-21 0.4864179172010097 1103 22-23 0.4534716224174602 1103 24-25 0.43655791841043623 1103 26-27 0.5381930951999294 1103 28-29 0.4421371637335696 1103 30-31 0.5101456496258336 1103 32-33 0.7758614901652976 1103 34-35 0.6566879447682368 1103 36-37 0.6756065889900071 1103 38-39 0.5711430596589722 1103 40-41 0.5744882328577177 1103 42-43 0.5592679796292757 1103 44-45 0.506299613010512 1103 46-47 0.44223207755204896 1103 48-49 0.46274822513479563 1103 50-51 0.40331276803494376 1103 52-53 0.25692417429565495 1103 54-55 0.39218704999800735 1103 56-57 0.379901768043851 1103 58-59 0.37478871909650735 1103 60-61 0.342293433701073 1103 62-63 0.21980627121645568 1103 64-65 0.3846791038652029 1103 66-67 0.31276811762157664 1103 68-69 0.1707404627397331 1103 70-71 0.1253250183612984 1103 72-73 -0.21279091141467887 1103 74-75 -0.017559789164465656 1103 76-77 0.11657122086118932 1103 78-79 0.03512976911840937 1103 80-81 0.061021182655789374 1103 82-83 0.05980129445479321 1103 84-85 0.16015642499386828 1103 86-87 0.42279347018388336 1103 88-89 0.4775671555779404 1103 90-91 0.1210505208038768 1103 92-93 -0.12079945252649082 1103 94-95 -0.25339141218990235 1103 96-97 -0.4249492867000626 1103 98-99 -0.16875123566405748 1103 100-101 -0.5481308569672727 1104 1 0.18787962037938044 1104 2 0.1455429427954016 1104 3 0.2277844970554881 1104 4 0.1850753747771492 1104 5 0.1054180018478661 1104 6 -0.06021627050548517 1104 7 -0.015164578013802554 1104 8 0.04245851207755891 1104 9 0.17512803470548732 1104 10-11 0.17184823294009277 1104 12-13 0.10735692799040208 1104 14-15 0.39291681514470866 1104 16-17 0.19306320473260996 1104 18-19 0.330963046457164 1104 20-21 0.2229044889613192 1104 22-23 0.30114029574777135 1104 24-25 0.3279267406629458 1104 26-27 0.30889706814151907 1104 28-29 0.39310010986916666 1104 30-31 0.2730312066958547 1104 32-33 0.16799858693948977 1104 34-35 0.07946294603029713 1104 36-37 0.26699497305785513 1104 38-39 0.06163507086599651 1104 40-41 0.18919934901703783 1104 42-43 0.18491162645647563 1104 44-45 0.2801113904853949 1104 46-47 -0.050490611629633975 1104 48-49 0.015438921283006835 1104 50-51 0.08019732808340763 1104 52-53 0.2973161678339409 1104 54-55 0.33291948222578327 1104 56-57 0.2114524520763652 1104 58-59 0.08659111952056264 1104 60-61 -0.0755422091885194 1104 62-63 -0.013875388635121055 1104 64-65 -5.892716206901127E-4 1104 66-67 0.01170389176464326 1104 68-69 -0.07451106434391264 1104 70-71 -0.12432949473880939 1104 72-73 -0.34594061609437077 1104 74-75 -0.14236732614260816 1104 76-77 -0.18804726063711996 1104 78-79 -0.2969415116125731 1104 80-81 -0.3959396684724865 1104 82-83 -0.4582776090739067 1104 84-85 -0.5065386378563481 1104 86-87 -0.24457363567708512 1104 88-89 -0.30189703998972917 1104 90-91 -0.2358689981016724 1104 92-93 -0.27185295093630657 1104 94-95 -0.442076164070361 1104 96-97 -0.07631356556320767 1104 98-99 0.10369910256603987 1104 100-101 -0.2020192410351207 1105 1 0.21549610923565865 1105 2 0.24035326501909537 1105 3 0.16125444569695446 1105 4 0.131856295141354 1105 5 0.03876692115362346 1105 6 0.19873689339680567 1105 7 0.018340251131867547 1105 8 0.1452642878079402 1105 9 0.2727566114606361 1105 10-11 0.29989426317788315 1105 12-13 0.3182975288972969 1105 14-15 0.43284747510790567 1105 16-17 0.40194555519747865 1105 18-19 0.4141030586051997 1105 20-21 0.21808661931817852 1105 22-23 0.19652622146432464 1105 24-25 0.20033776530782887 1105 26-27 0.1259849068733132 1105 28-29 0.21759253839420012 1105 30-31 0.17678056020374555 1105 32-33 0.08513671674307943 1105 34-35 0.21582466888664698 1105 36-37 0.31640275622139313 1105 38-39 0.25339928767839837 1105 40-41 0.19296178271401487 1105 42-43 0.2900628154005531 1105 44-45 0.1805207853687918 1105 46-47 -0.01422879435536828 1105 48-49 0.14537481332182978 1105 50-51 0.18039420748105783 1105 52-53 0.010817929900795775 1105 54-55 0.20941430728201027 1105 56-57 0.258436641585746 1105 58-59 0.392867881286719 1105 60-61 0.27829381936538056 1105 62-63 0.4840919199130056 1105 64-65 0.37820736601898375 1105 66-67 0.25392982421673693 1105 68-69 0.3612479173859171 1105 70-71 0.4076301431053331 1105 72-73 0.40188912494795304 1105 74-75 0.3835727936538831 1105 76-77 0.3250442044643336 1105 78-79 0.26793021232627723 1105 80-81 0.32678061995320107 1105 82-83 0.23317576531418283 1105 84-85 0.422320920003191 1105 86-87 0.3452060758972273 1105 88-89 0.3031041778209804 1105 90-91 0.4180047675494514 1105 92-93 0.32154373361163024 1105 94-95 0.2887083616149191 1105 96-97 0.397969579936607 1105 98-99 0.1802110671803696 1105 100-101 -0.1180474341047244 1106 1 0.1552615345377646 1106 2 0.11097810878286651 1106 3 0.14289912730739474 1106 4 0.17621148926441066 1106 5 0.059799749184684 1106 6 0.10704916644787943 1106 7 0.22316684878727955 1106 8 0.16705933188642774 1106 9 0.1401476362402434 1106 10-11 0.13216555140247976 1106 12-13 0.189905306246402 1106 14-15 0.1729000138291994 1106 16-17 0.14573800990883967 1106 18-19 0.20964131318103085 1106 20-21 0.2790920798194989 1106 22-23 0.2458069207090432 1106 24-25 0.18381971270842 1106 26-27 0.10457638941603875 1106 28-29 0.3626700676871337 1106 30-31 0.39234139949392244 1106 32-33 0.5095277747567479 1106 34-35 0.2291912651837933 1106 36-37 -0.1658936020476034 1106 38-39 0.16319589520288247 1106 40-41 0.2144028117562513 1106 42-43 0.46460884530254276 1106 44-45 0.43744647544523474 1106 46-47 0.10845981257379123 1106 48-49 0.49111276127728587 1106 50-51 0.25386677709558825 1106 52-53 0.285918679690198 1106 54-55 -0.014354623491435348 1106 56-57 0.2761092166911183 1106 58-59 0.28562191597441 1106 60-61 0.26020184481400577 1106 62-63 0.34609577506450506 1106 64-65 -0.12470675571305634 1106 66-67 0.35779386221339493 1106 68-69 -0.39795575107683234 1106 70-71 0.08758826818390375 1106 72-73 0.181497064132504 1106 74-75 0.3329237179221174 1106 76-77 0.36314164096198454 1106 78-79 0.5535148645462407 1106 80-81 -0.15036192961974493 1106 82-83 0.11456085998069199 1106 84-85 0.25507482464454156 1106 86-87 0.4458680840150109 1106 88-89 0.5441869725457202 1106 90-91 0.36654976575047726 1106 92-93 0.1208085383037023 1106 94-95 0.17271569622849015 1106 96-97 0.3744293862253585 1106 98-99 -0.14924038290856956 1106 100-101 -0.6376797353036068 1107 1 0.2601128392596195 1107 2 0.2642630823980596 1107 3 0.17117680761491982 1107 4 0.21434632929334185 1107 5 0.20530632537362692 1107 6 0.10830225847109887 1107 7 0.04429220276711732 1107 8 0.20386984293358523 1107 9 0.15665764944282756 1107 10-11 0.18529882421832156 1107 12-13 0.28500087910688165 1107 14-15 0.3046415742849504 1107 16-17 0.27723634703468747 1107 18-19 0.27761494058052705 1107 20-21 0.23304925894449013 1107 22-23 0.35413967736223384 1107 24-25 0.29016427977089165 1107 26-27 0.23809616004660228 1107 28-29 0.16489184665717005 1107 30-31 0.2875745614514855 1107 32-33 0.37837849872778406 1107 34-35 0.22291427146485177 1107 36-37 0.13019976314561177 1107 38-39 0.039128590524448725 1107 40-41 0.27738917361015325 1107 42-43 0.33492094459464994 1107 44-45 0.38713656075319847 1107 46-47 0.31761220131861734 1107 48-49 0.3555396701224751 1107 50-51 0.32756107149011626 1107 52-53 0.5454355981286199 1107 54-55 0.4912462975685159 1107 56-57 0.37581382076967884 1107 58-59 0.18078655476232086 1107 60-61 0.10247270265624309 1107 62-63 0.2964073180298854 1107 64-65 0.2571691372930971 1107 66-67 -0.14146900487743963 1107 68-69 -0.06014771907583594 1107 70-71 0.14911868229452097 1107 72-73 0.05922967994005646 1107 74-75 0.09940659323573087 1107 76-77 -0.09429902543601543 1107 78-79 0.17604718237977224 1107 80-81 0.38323104546631725 1107 82-83 0.2710911858931695 1107 84-85 0.29127541163708415 1107 86-87 0.21578051004729915 1107 88-89 0.320159628408792 1107 90-91 0.3044372439214378 1107 92-93 0.27656713049857373 1107 94-95 0.055176749965578864 1107 96-97 0.18934796577397606 1107 98-99 -0.20407723670454914 1107 100-101 -0.5311923551004298 1108 1 0.20238975034663298 1108 2 0.3287505957930392 1108 3 0.26853806917201695 1108 4 0.11202190917258292 1108 5 0.10575864182611383 1108 6 0.15339261531143222 1108 7 0.2137299071595109 1108 8 0.05843955521489619 1108 9 0.16571688154355257 1108 10-11 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66-67 -0.35363932064635506 2316 68-69 -0.3209853900026651 2316 70-71 -0.5709184843963122 2316 72-73 -0.48842476452740513 2316 74-75 -0.34269800022399366 2316 76-77 -0.2235814064715349 2316 78-79 0.02551054389389762 2316 80-81 -0.5414031319099415 2316 82-83 -0.29180367320778444 2316 84-85 -0.3492670563377196 2316 86-87 -0.3249865560034735 2316 88-89 -0.22127590543331266 2316 90-91 -0.31747630801280025 2316 92-93 -0.1915053208754962 2316 94-95 -0.2657037691844373 2316 96-97 -0.11063220804540919 2316 98-99 0.15624347396045835 2316 100-101 0.3706952748424328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 21.0 5 43.0 6 82.0 7 233.0 8 529.0 9 961.0 10 1550.0 11 2562.0 12 3686.0 13 5259.0 14 6646.0 15 9293.0 16 12123.0 17 15857.0 18 14088.0 19 10204.0 20 9363.0 21 9750.0 22 10556.0 23 12017.0 24 13988.0 25 16636.0 26 19735.0 27 24238.0 28 28694.0 29 34691.0 30 40955.0 31 48839.0 32 59134.0 33 72934.0 34 92762.0 35 121045.0 36 162400.0 37 201680.0 38 116615.0 39 5860.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.31696501278867 20.215808336139162 15.458894393658234 22.00833225741393 2 14.94610756199509 21.356129098708386 41.17589948070434 22.521863858592187 3 19.11195721975675 26.128976998952773 30.247512094599898 24.511553686690583 4 12.565145480321338 17.767754674947682 37.52531560116114 32.14178424356984 5 12.791140592918794 38.70340293596237 34.26508458142561 14.24037188969322 6 30.452474178086813 37.34760008100992 17.977958549922366 14.221967190980894 7 27.759991223924928 33.632029298589075 20.805795584959156 17.802183892526834 8 25.97186592857625 36.2899986498346 19.797475190710863 17.940660230878283 9 26.616569904813343 16.840781745763856 20.625379734017418 35.91726861540538 10-11 24.463393640720987 27.624932491730235 27.55620063457774 20.35547323297104 12-13 25.970979882535612 25.024893674475123 28.18018801053129 20.823938432457975 14-15 22.00377202457301 26.93550428677513 26.83884088300817 24.22188280564369 16-17 20.700735840140418 29.845617025585636 28.15803685951529 21.29561027475866 18-19 20.877059002227774 27.53687639235806 31.30451292783366 20.281551677580502 20-21 22.019383311955714 26.717410382771888 30.99806757577803 20.265138729494364 22-23 21.357169378248837 27.19655032741511 30.938702491055153 20.507577803280903 24-25 21.12815886761342 27.09421731135821 31.417035923772847 20.360587897255527 26-27 20.408112457730034 27.725215474499613 31.167532953061876 20.69913911470847 28-29 20.448676837912643 27.715773307230133 30.970431377843788 20.865118477013432 30-31 20.794867683791267 27.333760885708497 31.0438888138797 20.827482616620536 32-33 20.322351988118545 27.71083676500371 31.014564909201376 20.952246337676364 34-35 20.820023546191464 27.849532240981347 30.958051489359907 20.372392723467282 36-37 20.685302051113062 27.403685699928147 30.896691629266364 21.014320619692423 38-39 20.817652814645193 27.719106371828612 30.68115793431081 20.782082879215384 40-41 21.42182322528432 27.241794422903844 30.782596881022034 20.553785470789805 42-43 20.683566308168114 27.70884404258102 30.73466184543073 20.87292780382014 44-45 20.702643245225158 27.994805236449928 30.38426145288828 20.918290065436636 46-47 21.131143252548437 27.829482549112267 29.76215992709107 21.27721427124823 48-49 21.139666171606024 27.970659218254234 29.526007560926214 21.36366704921353 50-51 21.135784446094647 28.47975595760481 29.205933976912174 21.178525619388374 52-53 21.26464085600486 28.337777627759404 29.221123337608855 21.17645817862688 54-55 20.930432728009183 28.143522581516233 29.518539458583675 21.407505231890905 56-57 21.129793087153175 28.33524606764329 28.97978127320597 21.55517957199757 58-59 21.152113008843585 28.146138526969555 28.947630459731315 21.75411800445555 60-61 20.89490650104638 27.972431310335516 29.599254033619115 21.533408154998988 62-63 20.965747991628973 28.041205360156617 29.54364409640181 21.449402551812597 64-65 20.898408492540334 28.124324917302367 29.10323702153514 21.874029568622156 66-67 20.76165490051796 28.458074762159523 28.963628170573436 21.816642166749084 68-69 21.13810812909062 27.896724803127015 29.324750132486322 21.640416935296045 70-71 21.120959361235943 27.94428197902431 28.980197689779697 21.954560969960045 72-73 21.03076689394451 28.147741848376427 28.809533855397284 22.01195740228178 74-75 20.769509889961522 28.37651049753595 28.952271653277528 21.901707959225007 76-77 20.805795584959156 27.795306487544725 29.502042125160333 21.896855802335786 78-79 20.66023087828259 27.98074326605009 29.176736650239654 22.182289205427665 80-81 20.759679854990463 28.2639909436548 29.018447282358057 21.95788191899668 82-83 20.630877478414085 28.218098751763666 29.152546091582337 21.998477678239915 84-85 20.636306366481445 27.902676797362318 29.170040494371424 22.290976341784816 86-87 20.483287060868687 28.269466080455018 28.777394283592546 22.469852575083753 88-89 20.58345759084722 28.02321273814617 28.79609869379826 22.597230977208348 90-91 20.47030300370801 28.050416792545498 29.09725046877558 22.382029734970914 92-93 20.36396562279673 28.11069260301115 29.054635348740266 22.470706425451855 94-95 20.22145272073439 27.848504500064763 29.023740578892582 22.90630220030826 96-97 20.560403024370487 27.522910619050833 28.848984000540067 23.067702356038613 98-99 20.415083735711427 27.508738116414833 28.852745650350453 23.223432497523287 100-101 20.22294606089246 27.489958144872745 29.226144265172483 23.060951529062308 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 502.0 1 340.5 2 229.0 3 291.5 4 266.0 5 266.0 6 463.0 7 936.0 8 1429.5 9 1665.0 10 1467.0 11 1219.0 12 1301.5 13 1476.5 14 1696.5 15 1907.0 16 2022.5 17 2174.0 18 2413.5 19 2655.5 20 2970.0 21 3393.0 22 4017.0 23 4696.5 24 5437.0 25 6477.5 26 7855.5 27 9743.5 28 11698.5 29 13989.0 30 16990.5 31 20043.0 32 23814.5 33 27526.0 34 31325.0 35 35291.5 36 38419.5 37 40662.0 38 41660.0 39 41392.0 40 41149.0 41 41792.0 42 42508.5 43 43149.0 44 43376.0 45 43784.5 46 43355.0 47 43213.5 48 43706.0 49 42670.0 50 40898.0 51 38227.0 52 34968.0 53 32756.0 54 31341.0 55 28566.5 56 24653.0 57 21494.5 58 18984.5 59 16205.5 60 13118.5 61 10568.0 62 8608.0 63 6394.0 64 4526.5 65 3351.5 66 2415.0 67 1768.0 68 1362.0 69 1034.0 70 759.5 71 582.0 72 469.5 73 404.5 74 331.5 75 259.0 76 164.0 77 94.0 78 61.5 79 40.5 80 27.5 81 14.5 82 5.5 83 4.5 84 3.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.906973604266523E-4 2 0.04649632079929791 3 5.906973604266523E-4 4 0.0 5 0.08717005333153312 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.5315601161142237E-4 26-27 0.008185377708769325 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011392020522514009 36-37 4.219266860190373E-5 38-39 0.004261459528792277 40-41 0.005780395598460811 42-43 4.2192668601903735E-4 44-45 4.219266860190373E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6877067440761493E-4 68-69 0.0010126240464456895 70-71 1.2657800580571118E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012235873894552083 82-83 6.750826976304597E-4 84-85 0.005105312900830352 86-87 0.002531560116114224 88-89 8.438533720380746E-5 90-91 0.0032066428137446836 92-93 8.860460406399784E-4 94-95 1.2657800580571118E-4 96-97 0.0 98-99 1.6877067440761493E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1185040.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.3389414819826 #Duplication Level Percentage of deduplicated Percentage of total 1 83.82906119542127 57.28789307523433 2 10.370817818383053 14.174614240215641 3 2.7306573272312105 5.598306938790023 4 1.0377988784460674 2.8368830729677197 5 0.55051266304787 1.88107263325594 6 0.3241027536508682 1.3289303469537666 7 0.20609407511431735 0.9858975657314466 8 0.15238796147029623 0.8331225585181756 9 0.11098446978701133 0.682610506756509 >10 0.6216263286568704 7.776082740792238 >50 0.041190005353022585 1.887392199156223 >100 0.023156704253383505 2.957925752574666 >500 0.0011144902048147696 0.4602788855063363 >1k 3.714967349382566E-4 0.8230690274746509 >5k 1.2383224497941885E-4 0.48592045607232637 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5742 0.48454060622426254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4981 0.420323364612165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3362 0.28370350367920066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1383 0.11670492135286573 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.438533720380746E-5 0.0 0.0 0.0 0.0 6 8.438533720380746E-5 0.0 0.0 0.0 0.0 7 2.531560116114224E-4 0.0 0.0 0.0 0.0 8 4.2192668601903735E-4 0.0 0.0 0.0 0.0 9 4.2192668601903735E-4 0.0 0.0 0.0 0.0 10-11 4.2192668601903735E-4 0.0 0.0 0.0 0.0 12-13 4.641193546209411E-4 0.0 0.0 0.0 0.0 14-15 8.016607034361709E-4 0.0 0.0 4.219266860190373E-5 0.0 16-17 9.282387092418822E-4 0.0 0.0 8.438533720380746E-5 0.0 18-19 0.0011813947208533047 0.0 0.0 8.438533720380746E-5 0.0 20-21 0.0013501653952609194 0.0 0.0 2.1096334300951868E-4 0.0 22-23 0.0014345507324647268 0.0 0.0 4.2192668601903735E-4 0.0 24-25 0.0019408627556875718 0.0 0.0 0.0010126240464456895 0.0 26-27 0.0019830554242894756 0.0 0.0 0.001898670087085668 0.0 28-29 0.0025737527847161275 0.0 0.0 0.00464119354620941 0.0 30-31 0.0026159454533180313 0.0 0.0 0.015315938702491056 0.0 32-33 0.0026159454533180313 0.0 0.0 0.03434483224194964 0.0 34-35 0.002911294133531358 0.0 0.0 0.0654830216701546 0.0 36-37 0.0032066428137446836 0.0 0.0 0.12235873894552082 0.0 38-39 0.0032066428137446836 0.0 0.0 0.20978194828866537 0.0 40-41 0.0032488354823465874 0.0 0.0 0.30209950718963074 0.0 42-43 0.00350199149395801 0.0 0.0 0.38893201917234865 0.0 44-45 0.0038395328427732398 0.0 0.0 0.49702963613042594 0.0 46-47 0.004430230203199891 0.0 0.0 0.6287129548369674 0.0 48-49 0.004725578883413218 0.0 0.0 0.7719148720718287 0.0 50-51 0.004725578883413218 0.0 0.0 0.9287872139337069 0.0 52-53 0.004725578883413218 0.0 0.0 1.0800479308715318 0.0 54-55 0.004725578883413218 0.0 0.0 1.2422787416458516 0.0 56-57 0.004894349557820833 0.0 0.0 1.4359430905285897 0.0 58-59 0.004894349557820833 0.0 0.0 1.6525180584621615 0.0 60-61 0.004894349557820833 0.0 0.0 1.8911175994059273 0.0 62-63 0.004894349557820833 0.0 0.0 2.1210676432863025 0.0 64-65 0.004978734895024641 0.0 0.0 2.3651944238169174 0.0 66-67 0.00531627624383987 0.0 0.0 2.6427377978802404 0.0 68-69 0.00531627624383987 0.0 0.0 2.950195773982313 0.0 70-71 0.005358468912441774 0.0 0.0 3.28811685681496 0.0 72-73 0.005485046918247485 0.0 0.0 3.6261645176534127 0.0 74-75 0.005569432255451293 0.0 0.0 3.972186592857625 0.0 76-77 0.005569432255451293 0.0 0.0 4.3360983595490445 0.0 78-79 0.005569432255451293 0.0 0.0 4.749248970498886 0.0 80-81 0.006413285627489367 0.0 0.0 5.194086275568758 0.0 82-83 0.006835212313508404 0.0 0.0 5.661538851009249 0.0 84-85 0.007130560993721731 0.0 0.0 6.122324984810639 0.0 86-87 0.007425909673935057 0.0 0.0 6.585516100722338 0.0 88-89 0.007425909673935057 0.0 0.0 7.083600553567813 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3140 0.0 53.02231 1 GTATCAA 5715 0.0 41.36761 1 TATCAAC 7540 0.0 31.165627 2 ATCAACG 7560 0.0 31.130314 3 TCAACGC 7735 0.0 30.303074 4 GGACCGA 160 1.9081199E-9 29.683952 6 CAACGCA 7920 0.0 29.59524 5 AACGCAG 8135 0.0 28.78267 6 GTACATG 7175 0.0 25.988693 1 ACGCAGA 9305 0.0 25.11253 7 GTCCTAC 1180 0.0 24.993666 1 TACATGG 7330 0.0 24.855076 2 CGCAGAG 9385 0.0 24.79725 8 GTAGGAC 1400 0.0 24.791048 3 ACATGGG 7320 0.0 23.447481 3 TGTAGGA 1925 0.0 22.734077 2 GCAGAGT 10125 0.0 22.562737 9 AGGACCG 275 5.0931703E-11 22.475573 5 TGGTATC 1435 0.0 22.209724 2 GAGTACT 5615 0.0 21.865152 12-13 >>END_MODULE