FastQCFastQC Report
Thu 26 May 2016
SRR938641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183121
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.49952625302061243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47650.4027483241359083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.27182342296350076No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.10937173797101057No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.052.164621
GTATCAA62250.039.3481641
GTCCTAC10950.030.8990361
ATCAACG79100.030.8196413
TATCAAC79900.030.5705382
TCAACGC80900.030.1339154
CAACGCA82750.029.4602245
AACGCAG84900.028.8188116
TAGCCCT8750.025.5256334
GTACATG73800.025.3122311
ACGCAGA97300.025.1896177
CGCAGAG98100.024.8873628
TACATGG74300.024.8798352
ATGGGCG1951.5625119E-824.369815
ACATGGG73750.023.9055423
GAGTACT54800.023.70667612-13
GCAGAGT100200.023.60739
TCCTACA16550.022.9709382
GTACTTT58600.021.8856814-15
CATGGGG34500.021.6255554
Produced by FastQC (version 0.11.5)