##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938641_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1183121 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.115584965527617 31.0 31.0 34.0 28.0 34.0 2 31.24282469840363 31.0 31.0 34.0 28.0 34.0 3 31.29401557406216 31.0 31.0 34.0 27.0 34.0 4 34.96710649206632 37.0 35.0 37.0 32.0 37.0 5 34.9895513645688 37.0 35.0 37.0 32.0 37.0 6 35.00821893956746 37.0 35.0 37.0 32.0 37.0 7 34.92989220882733 37.0 35.0 37.0 32.0 37.0 8 34.89326450971625 37.0 35.0 37.0 32.0 37.0 9 36.37751844485898 39.0 37.0 39.0 32.0 39.0 10-11 36.22790187985844 39.0 35.0 39.0 32.0 39.0 12-13 36.06079978294697 38.5 35.0 39.0 30.5 39.0 14-15 37.015097779517056 39.0 36.0 41.0 31.0 41.0 16-17 36.8684809077009 39.0 36.0 41.0 31.0 41.0 18-19 36.80221507352164 39.0 36.0 41.0 30.5 41.0 20-21 36.82813930274249 39.0 36.0 40.5 30.5 41.0 22-23 36.91420150601671 39.0 36.0 41.0 30.5 41.0 24-25 36.8342164495432 39.0 36.0 41.0 30.0 41.0 26-27 36.65737274547574 39.0 36.0 41.0 30.0 41.0 28-29 36.470706292932 39.0 35.5 41.0 30.0 41.0 30-31 36.19964990901184 39.0 35.0 40.0 29.5 41.0 32-33 35.88662909372752 38.0 35.0 40.0 28.5 41.0 34-35 35.637146580949874 38.0 35.0 40.0 27.0 41.0 36-37 35.344478713504365 38.0 34.0 40.0 26.5 41.0 38-39 35.02865640961491 38.0 34.0 40.0 25.5 41.0 40-41 34.8742495484401 38.0 34.0 40.0 25.0 41.0 42-43 34.636536753214585 38.0 33.5 40.0 24.0 41.0 44-45 34.27854716466025 38.0 33.0 40.0 23.5 41.0 46-47 33.79045084991307 38.0 33.0 40.0 20.5 41.0 48-49 33.353613873813416 38.0 32.5 40.0 17.5 41.0 50-51 32.584063675651095 37.0 31.5 39.5 15.0 40.5 52-53 32.618802303399235 37.0 31.5 39.5 14.5 40.0 54-55 32.71776555398814 37.0 31.5 40.0 11.0 41.0 56-57 32.423130432136695 37.0 31.0 40.0 8.5 41.0 58-59 32.025416250746964 37.0 31.0 40.0 7.5 41.0 60-61 31.573543196342555 36.0 30.0 39.0 4.0 41.0 62-63 31.02110096938521 35.5 29.0 39.0 2.0 40.5 64-65 30.550104765277602 35.0 28.5 39.0 2.0 40.0 66-67 30.00624196510754 35.0 28.0 38.5 2.0 40.0 68-69 29.414242921898943 34.5 27.5 38.0 2.0 40.0 70-71 28.79613708149885 34.0 26.0 37.5 2.0 39.5 72-73 28.170182508805098 34.0 26.0 37.0 2.0 39.0 74-75 27.476350263413465 33.0 24.5 36.0 2.0 39.0 76-77 26.748241726754912 32.5 22.5 36.0 2.0 38.5 78-79 25.968051027747798 32.0 20.5 35.0 2.0 37.5 80-81 25.199118686930582 31.5 17.5 35.0 2.0 37.0 82-83 24.465294758524273 31.0 10.5 35.0 2.0 36.0 84-85 23.70562689699532 30.5 4.5 34.0 2.0 36.0 86-87 22.960898758453276 30.0 2.0 34.0 2.0 35.5 88-89 22.16481154505752 29.5 2.0 34.0 2.0 35.0 90-91 21.33995212662103 29.0 2.0 34.0 2.0 35.0 92-93 20.484188007819995 28.0 2.0 34.0 2.0 35.0 94-95 19.567906410248824 26.5 2.0 34.0 2.0 35.0 96-97 18.460749576755042 25.0 2.0 33.0 2.0 35.0 98-99 17.204889018113953 20.5 2.0 33.0 2.0 35.0 100-101 14.435461377154155 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6429375509408608 1101 2 0.5940372585555451 1101 3 0.491443773400956 1101 4 0.3241261900673109 1101 5 0.48607512895739546 1101 6 0.4044695470024138 1101 7 0.500152078913068 1101 8 0.5500295420038057 1101 9 0.5690421339697238 1101 10-11 0.5656209678448931 1101 12-13 0.7286639781636026 1101 14-15 0.7809439267491385 1101 16-17 1.0400189667405826 1101 18-19 0.8751034906135047 1101 20-21 0.8550467576872123 1101 22-23 0.9295321996682304 1101 24-25 1.0130807976503746 1101 26-27 1.0152344992388223 1101 28-29 0.9922180914561451 1101 30-31 0.9132456289602615 1101 32-33 0.9390668684555052 1101 34-35 0.9080621762136332 1101 36-37 0.920985187565492 1101 38-39 1.279948342015075 1101 40-41 1.1766883909745758 1101 42-43 1.2470226242283289 1101 44-45 1.4209958417016324 1101 46-47 1.4975530562933912 1101 48-49 1.5206102782691318 1101 50-51 1.3550468433289637 1101 52-53 1.6092876649346195 1101 54-55 1.816080295934512 1101 56-57 1.9588139428075877 1101 58-59 2.1602218207462442 1101 60-61 2.0410734508536876 1101 62-63 1.9512157405422101 1101 64-65 2.271307341820414 1101 66-67 2.2163904144404682 1101 68-69 2.3674577692294996 1101 70-71 2.4415735515761945 1101 72-73 2.4959562220870133 1101 74-75 2.973962421195065 1101 76-77 3.1839638004985638 1101 78-79 3.5055872894281492 1101 80-81 3.9971356530250617 1101 82-83 4.095013630770751 1101 84-85 4.006838788245226 1101 86-87 4.159683377174677 1101 88-89 4.5457370612361565 1101 90-91 5.030836391317848 1101 92-93 5.045526078941236 1101 94-95 5.280517168196134 1101 96-97 5.466492770693591 1101 98-99 5.602685831380409 1101 100-101 5.560743841382189 1102 1 0.639151442727357 1102 2 0.6008982042463273 1102 3 0.4897721111137088 1102 4 0.2781312693150184 1102 5 0.2796736923313574 1102 6 0.3945267215498376 1102 7 0.37009252759462186 1102 8 0.44589528084948427 1102 9 0.6609369046333313 1102 10-11 0.5666381378148344 1102 12-13 0.8031462602159039 1102 14-15 0.9967837851992201 1102 16-17 0.8406769802001506 1102 18-19 0.9695039941368435 1102 20-21 0.8433895760975076 1102 22-23 0.6495657385482403 1102 24-25 0.7969856563865463 1102 26-27 0.9623368192314317 1102 28-29 0.7680023158585456 1102 30-31 0.8279600423159579 1102 32-33 0.9911317983187473 1102 34-35 0.8568559649186867 1102 36-37 0.7871892641679423 1102 38-39 0.9888700362126741 1102 40-41 1.2802465052268417 1102 42-43 1.3048309772580566 1102 44-45 1.443373140897485 1102 46-47 1.4996591395067966 1102 48-49 1.4288554730103087 1102 50-51 1.5364186803528952 1102 52-53 1.4386385863735356 1102 54-55 1.5978104091832463 1102 56-57 1.775406005272643 1102 58-59 1.9000697074006823 1102 60-61 1.9998862097126633 1102 62-63 2.199108556971858 1102 64-65 2.1859187617650484 1102 66-67 2.2778061777140692 1102 68-69 2.510990091114717 1102 70-71 2.589216366905486 1102 72-73 2.656127261572223 1102 74-75 2.7111298383051725 1102 76-77 2.8977534738686046 1102 78-79 3.1959191130717386 1102 80-81 3.7422031929350084 1102 82-83 3.600742089082626 1102 84-85 3.6754346007267955 1102 86-87 4.053695674764015 1102 88-89 4.288115768888918 1102 90-91 4.430260684558576 1102 92-93 4.368472482754633 1102 94-95 4.753867403012311 1102 96-97 4.972223429719378 1102 98-99 4.9725893080682475 1102 100-101 4.7354426736833855 1103 1 0.5657474684398807 1103 2 0.5143737978945104 1103 3 0.5240496067842599 1103 4 0.36480830709129464 1103 5 0.49069250708964063 1103 6 0.6541469041205019 1103 7 0.5583399079110336 1103 8 0.5188707130605863 1103 9 0.5438686020708801 1103 10-11 0.4649885443427664 1103 12-13 0.53030878333076 1103 14-15 0.7316571304691095 1103 16-17 0.8025572819407358 1103 18-19 0.8959855462810253 1103 20-21 0.8094144080561776 1103 22-23 0.6761030598667475 1103 24-25 0.711177443641823 1103 26-27 0.7624571632391834 1103 28-29 1.0195095511751049 1103 30-31 0.9632237718061063 1103 32-33 0.8420050261798551 1103 34-35 1.1109726105496165 1103 36-37 1.1389401227197027 1103 38-39 1.0613611571730743 1103 40-41 1.0144535556245557 1103 42-43 1.2613310212863453 1103 44-45 1.1309600967811377 1103 46-47 1.1259909954462088 1103 48-49 1.0825611878830657 1103 50-51 1.2174336793255662 1103 52-53 1.3220164372056686 1103 54-55 1.471513775880247 1103 56-57 1.510490686361031 1103 58-59 1.6897993736661974 1103 60-61 1.910074357295514 1103 62-63 1.9711887471740965 1103 64-65 2.0559531038355523 1103 66-67 2.047315115458062 1103 68-69 2.3294350117170275 1103 70-71 2.3213687635123748 1103 72-73 2.3788605381579764 1103 74-75 2.8824674179230065 1103 76-77 2.821649097456028 1103 78-79 3.095514052752975 1103 80-81 3.4654970964836878 1103 82-83 3.4398773733586587 1103 84-85 3.7293898092741564 1103 86-87 3.6729771329811634 1103 88-89 4.219220292062477 1103 90-91 4.408560605307642 1103 92-93 4.31873224969819 1103 94-95 4.869890767361131 1103 96-97 5.150567378763963 1103 98-99 5.328009191246505 1103 100-101 5.18586974814991 1104 1 0.5950723157386335 1104 2 0.6197094792630793 1104 3 0.6931228462070713 1104 4 0.5925377755324917 1104 5 0.451601150984942 1104 6 0.5123360854065737 1104 7 0.5249163451160683 1104 8 0.5312248525345353 1104 9 0.6659969282953071 1104 10-11 0.7939929599776647 1104 12-13 0.7294669048807876 1104 14-15 0.8358545974008607 1104 16-17 1.050255532186192 1104 18-19 1.1446382303760814 1104 20-21 1.1262619420474138 1104 22-23 0.8921453081649346 1104 24-25 1.0238519330993512 1104 26-27 1.0570492765198978 1104 28-29 0.9650904856953062 1104 30-31 1.0539468544845008 1104 32-33 1.196067720680979 1104 34-35 1.2859693024990477 1104 36-37 1.4092495112122592 1104 38-39 1.410123211137595 1104 40-41 1.4558308155131812 1104 42-43 1.5264655568442222 1104 44-45 1.497302601718708 1104 46-47 1.6193900051547203 1104 48-49 1.9648105152461213 1104 50-51 1.9926336734445869 1104 52-53 2.123181769489136 1104 54-55 2.229369783381763 1104 56-57 2.52494972616055 1104 58-59 2.637821322785932 1104 60-61 2.8589274030707656 1104 62-63 3.1230613488374246 1104 64-65 3.065686940537102 1104 66-67 3.3541731535308656 1104 68-69 3.265007663858764 1104 70-71 3.4086526988020047 1104 72-73 3.5625284066135485 1104 74-75 3.59656200039802 1104 76-77 3.5333316618241604 1104 78-79 3.77770228328081 1104 80-81 4.424724352206617 1104 82-83 4.488378703930106 1104 84-85 4.378474255228777 1104 86-87 4.779562522342797 1104 88-89 5.145914708236479 1104 90-91 5.427561067617923 1104 92-93 5.488101171665768 1104 94-95 5.524627895777204 1104 96-97 5.708309605661928 1104 98-99 5.939349188600065 1104 100-101 5.985286306045696 1105 1 0.5894830151675059 1105 2 0.6475824798448429 1105 3 0.6727157999976008 1105 4 0.6110243793521093 1105 5 0.5929084905779547 1105 6 0.6222720289568286 1105 7 0.6597070043845505 1105 8 0.6640137477484416 1105 9 0.7167197527843925 1105 10-11 0.6982077680272667 1105 12-13 0.6746029310038111 1105 14-15 0.8376514934154713 1105 16-17 0.7272005274632747 1105 18-19 0.8812326667231076 1105 20-21 0.8753806966999562 1105 22-23 0.837275078775285 1105 24-25 0.9630444402462786 1105 26-27 0.993909259379798 1105 28-29 1.1480798700903065 1105 30-31 1.1399781383033698 1105 32-33 0.982415377053151 1105 34-35 1.2592809396292637 1105 36-37 1.572541068146812 1105 38-39 1.5147888852629166 1105 40-41 1.466893689671032 1105 42-43 1.5172621338276855 1105 44-45 1.7525728711436415 1105 46-47 1.862267322040232 1105 48-49 1.7961478187448208 1105 50-51 1.8257999324603489 1105 52-53 1.9819706084034294 1105 54-55 2.1473748691135555 1105 56-57 2.267009916703465 1105 58-59 2.146897289731804 1105 60-61 2.483192380331971 1105 62-63 2.7059226516105 1105 64-65 2.8172383076794 1105 66-67 3.1093448986312353 1105 68-69 3.179212525643205 1105 70-71 3.3001132984554538 1105 72-73 3.5213514944597115 1105 74-75 3.710781406249424 1105 76-77 3.694030308756769 1105 78-79 3.8946967909406816 1105 80-81 3.996819917673683 1105 82-83 4.253349709628882 1105 84-85 4.460570039638785 1105 86-87 4.629833698817066 1105 88-89 4.804036865887905 1105 90-91 5.078285299597354 1105 92-93 5.342348508381249 1105 94-95 5.550725894638699 1105 96-97 5.745742593482447 1105 98-99 5.976273134077015 1105 100-101 5.858776381674845 1106 1 0.23316263712620255 1106 2 0.20168141660367667 1106 3 0.16553406202448784 1106 4 0.12501325345299108 1106 5 0.3342889095936741 1106 6 0.039640544456815974 1106 7 0.22286442688458408 1106 8 0.1296475800524277 1106 9 0.2414001074971992 1106 10-11 0.23696111402178843 1106 12-13 0.11768513405147019 1106 14-15 0.4258496660164539 1106 16-17 0.1849759458060518 1106 18-19 0.453149491654699 1106 20-21 0.4612254575080854 1106 22-23 0.31254313219157126 1106 24-25 0.23847811104527494 1106 26-27 0.31651098509049547 1106 28-29 0.07698552426027305 1106 30-31 0.4212394473910095 1106 32-33 0.5652442565375324 1106 34-35 0.46561963652328586 1106 36-37 0.6808701644665902 1106 38-39 0.6337148770979368 1106 40-41 0.7515821196331984 1106 42-43 0.5589992096901639 1106 44-45 0.7836334205596742 1106 46-47 0.9813940735468591 1106 48-49 0.8637914908897031 1106 50-51 0.44517641853053647 1106 52-53 0.8676748000560153 1106 54-55 0.8013051976565748 1106 56-57 0.9737288232548806 1106 58-59 0.16561429710419873 1106 60-61 1.3983988973572963 1106 62-63 1.6940374547389183 1106 64-65 1.7632590042444285 1106 66-67 1.7373540505805138 1106 68-69 1.3403387986165498 1106 70-71 1.9553008984614344 1106 72-73 2.4561707733698555 1106 74-75 2.4353481093045595 1106 76-77 2.665068202612101 1106 78-79 2.9901181993239874 1106 80-81 3.4960965099184556 1106 82-83 3.891408808490869 1106 84-85 3.954327423257997 1106 86-87 4.016186527397469 1106 88-89 4.023413725812237 1106 90-91 4.153567185616701 1106 92-93 4.5303877929739755 1106 94-95 4.5239332640514505 1106 96-97 4.630592528431826 1106 98-99 4.935772393845827 1106 100-101 4.305372065902686 1107 1 0.5037687294532169 1107 2 0.32754763663926667 1107 3 0.4854335700324448 1107 4 0.4742647974705747 1107 5 0.3720606392422994 1107 6 0.46948457251083653 1107 7 0.46388818905354157 1107 8 0.4347454550655101 1107 9 0.41637132072864347 1107 10-11 0.36937501541402895 1107 12-13 0.41420804134063616 1107 14-15 0.7294052680960803 1107 16-17 0.8234839188686109 1107 18-19 0.6902338281633007 1107 20-21 0.6995676885698785 1107 22-23 0.7431984237230225 1107 24-25 0.7759654623136356 1107 26-27 0.8209127437003545 1107 28-29 0.9271739467453557 1107 30-31 0.970843411241809 1107 32-33 1.0764920321054205 1107 34-35 0.99316595705087 1107 36-37 0.9801775373686468 1107 38-39 0.9814555519295851 1107 40-41 0.6400063493690595 1107 42-43 0.6385745612376752 1107 44-45 0.9152325691691914 1107 46-47 0.9442940351645035 1107 48-49 1.116066517931813 1107 50-51 1.0389532425648724 1107 52-53 1.0804317001571988 1107 54-55 1.3947906647000892 1107 56-57 1.4383804161227474 1107 58-59 1.508424583576165 1107 60-61 1.4127800690660024 1107 62-63 1.7372525006232706 1107 64-65 1.9089340103507162 1107 66-67 2.1232241088519146 1107 68-69 2.263736335167021 1107 70-71 2.3195093495588175 1107 72-73 2.5135698452146507 1107 74-75 2.56548523900204 1107 76-77 2.9531766502201826 1107 78-79 2.993099810685166 1107 80-81 3.4667095808560298 1107 82-83 3.7345924506277193 1107 84-85 4.000988158105688 1107 86-87 4.128556114612653 1107 88-89 4.429123973901845 1107 90-91 4.601368923290735 1107 92-93 4.909392643108312 1107 94-95 5.373896626346017 1107 96-97 5.264499852483606 1107 98-99 5.40417673454159 1107 100-101 5.053433756820025 1108 1 0.4713617719175076 1108 2 0.6241563238416781 1108 3 0.45983838977785396 1108 4 0.4690174940015268 1108 5 0.4528464391321734 1108 6 0.45353860030211024 1108 7 0.3079558209649207 1108 8 0.39945248062182515 1108 9 0.5940119776132988 1108 10-11 0.6222901840079444 1108 12-13 0.5573735905260051 1108 14-15 0.7098963120503399 1108 16-17 0.8094360453221512 1108 18-19 0.8809182449676243 1108 20-21 0.7669194198561158 1108 22-23 0.8015843920448589 1108 24-25 0.961951599067433 1108 26-27 0.7477371533787149 1108 28-29 0.8519577384079753 1108 30-31 0.8241650839007661 1108 32-33 0.9966892918469412 1108 34-35 0.9984958569634088 1108 36-37 1.2426026337311598 1108 38-39 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2311 24-25 0.1273594644612075 2311 26-27 0.2615075435162808 2311 28-29 0.1984272064477679 2311 30-31 0.45233980639444127 2311 32-33 0.6073441566644036 2311 34-35 0.5003181276291784 2311 36-37 0.4201280665909195 2311 38-39 0.6027720882081482 2311 40-41 0.4435210755540595 2311 42-43 0.6189703274594862 2311 44-45 0.998688620710837 2311 46-47 0.9441693994841884 2311 48-49 0.8274697239450077 2311 50-51 0.894456387218959 2311 52-53 0.7435932279988933 2311 54-55 0.83199729148334 2311 56-57 1.0450858157239153 2311 58-59 1.0223300905557622 2311 60-61 0.91321150026711 2311 62-63 0.965197737622816 2311 64-65 1.2522017661414786 2311 66-67 1.1842016360800152 2311 68-69 1.2969891346761493 2311 70-71 1.4336871375631155 2311 72-73 1.5629715939491184 2311 74-75 1.4625112399224243 2311 76-77 1.544179871573153 2311 78-79 1.6074731808339635 2311 80-81 1.9244695900598963 2311 82-83 1.904802605559457 2311 84-85 2.525922964057518 2311 86-87 2.936045760263827 2311 88-89 2.7506092477168487 2311 90-91 2.9353062603056124 2311 92-93 3.1749490934841376 2311 94-95 3.513037982382219 2311 96-97 3.6974592787759804 2311 98-99 3.645680416087842 2311 100-101 3.728514290644233 2312 1 -0.05087208899144002 2312 2 -0.22698198008547266 2312 3 0.07569229936745714 2312 4 -0.07957536859066039 2312 5 -0.004893422091626576 2312 6 0.12577488313430507 2312 7 0.004654739576189115 2312 8 -0.09605000790431717 2312 9 0.08429703797973787 2312 10-11 -0.06597045126252965 2312 12-13 0.15250158916972367 2312 14-15 0.20409822779162567 2312 16-17 0.23619859996679082 2312 18-19 0.24123896815390822 2312 20-21 0.177098763272717 2312 22-23 0.19019227056119092 2312 24-25 -0.18781922644510018 2312 26-27 0.04420023721358035 2312 28-29 0.16139627605071638 2312 30-31 0.3781536887518726 2312 32-33 0.327993255324337 2312 34-35 0.3673226773414697 2312 36-37 0.3270632714235546 2312 38-39 0.35837439938553217 2312 40-41 0.47705011342372217 2312 42-43 0.6020905125066136 2312 44-45 0.6108184786757036 2312 46-47 0.43494691281789954 2312 48-49 0.7352556102786636 2312 50-51 0.7985277106499851 2312 52-53 1.0375992687933788 2312 54-55 1.0364882207333963 2312 56-57 1.0870762670630185 2312 58-59 1.3364776885753002 2312 60-61 1.16850077976855 2312 62-63 1.0847591109477435 2312 64-65 1.3122620307129829 2312 66-67 1.4369173650853853 2312 68-69 1.6525333796724198 2312 70-71 1.854972813615948 2312 72-73 1.88195087173008 2312 74-75 2.015613120979946 2312 76-77 2.121128314539259 2312 78-79 2.1518878354955078 2312 80-81 2.468343010564187 2312 82-83 2.732360384993992 2312 84-85 2.9489123926671788 2312 86-87 3.2341594457219536 2312 88-89 3.1009454580741327 2312 90-91 3.3187497521142184 2312 92-93 3.306189415787287 2312 94-95 3.2777349390452564 2312 96-97 3.5026185664974925 2312 98-99 3.803901201391124 2312 100-101 3.6653965907340815 2313 1 -0.4220142010218275 2313 2 -0.49588012230200107 2313 3 -0.46667915737285526 2313 4 -0.3628419144037167 2313 5 -0.22848622721291179 2313 6 -0.24269232055854673 2313 7 -0.2497761807740133 2313 8 -0.16228323719450088 2313 9 -0.2478016349022596 2313 10-11 -0.3351803821578869 2313 12-13 -0.4073567258765465 2313 14-15 -0.20782175439836692 2313 16-17 -0.41890916873938977 2313 18-19 -0.27612294751207855 2313 20-21 -0.21811979547985771 2313 22-23 -0.2612220801232681 2313 24-25 -0.17059474768308291 2313 26-27 -0.014250769369759553 2313 28-29 -0.12395624392875249 2313 30-31 0.11998300801894146 2313 32-33 -0.0518028382872302 2313 34-35 -0.17384705837076808 2313 36-37 0.12662290540976784 2313 38-39 0.04601495985549775 2313 40-41 0.042875575370736385 2313 42-43 0.05109233439746674 2313 44-45 0.17813132595944836 2313 46-47 0.057805282384599366 2313 48-49 0.24672932007553072 2313 50-51 0.25330301834962654 2313 52-53 0.34214922540420645 2313 54-55 0.3076438492857463 2313 56-57 0.629457378461872 2313 58-59 0.6167825258999855 2313 60-61 0.5972107810861331 2313 62-63 0.7297823048450844 2313 64-65 0.8015731878635641 2313 66-67 0.974456381264325 2313 68-69 1.1582330357394106 2313 70-71 1.2510398464781396 2313 72-73 1.3327138169234267 2313 74-75 1.296459755025225 2313 76-77 1.4921591140358537 2313 78-79 1.6074167744826084 2313 80-81 1.8518658023734034 2313 82-83 2.144600106758091 2313 84-85 2.055804228687915 2313 86-87 2.159122303682615 2313 88-89 2.2291695461064442 2313 90-91 2.155909737757174 2313 92-93 2.2295592550883327 2313 94-95 2.4708195910913595 2313 96-97 2.477455118807569 2313 98-99 2.4786982263932806 2313 100-101 2.475454246083215 2314 1 -0.5665260307631073 2314 2 -0.5512425213063636 2314 3 -0.7592388987186744 2314 4 -0.5442159159709163 2314 5 -0.5807741446121426 2314 6 -0.6027800311924736 2314 7 -0.5531384217412025 2314 8 -0.6598106138431916 2314 9 -0.6298268813414865 2314 10-11 -0.5099810248424106 2314 12-13 -0.43167140423796724 2314 14-15 -0.4007225926538993 2314 16-17 -0.5202949572461364 2314 18-19 -0.5819018403299481 2314 20-21 -0.6068056504939108 2314 22-23 -0.5258553350965016 2314 24-25 -0.5690723228659778 2314 26-27 -0.5019732560847601 2314 28-29 -0.560265614874929 2314 30-31 -0.6450371087968705 2314 32-33 -0.3752736639656362 2314 34-35 -0.49040375750090703 2314 36-37 -0.4628080290508976 2314 38-39 -0.25449767324143835 2314 40-41 -0.2741371268674655 2314 42-43 -0.41335588537956625 2314 44-45 -0.40400026633096786 2314 46-47 -0.3618196427123692 2314 48-49 -0.26547246706155647 2314 50-51 -0.3316781818110428 2314 52-53 -0.3672385122277362 2314 54-55 -0.24945202001750744 2314 56-57 -0.066859367552631 2314 58-59 0.13697836030335964 2314 60-61 0.2923241398371097 2314 62-63 0.30892436051367866 2314 64-65 0.31013024257735466 2314 66-67 0.40829806752101305 2314 68-69 0.6874353430822104 2314 70-71 0.611952313636035 2314 72-73 0.7032550284100942 2314 74-75 0.770003343654448 2314 76-77 0.6266750922530626 2314 78-79 0.8935743638888454 2314 80-81 0.9385426097590077 2314 82-83 1.3338331655018294 2314 84-85 1.7030833552691114 2314 86-87 1.8771181288671563 2314 88-89 1.8517109156302922 2314 90-91 1.8454325313276954 2314 92-93 2.0259053038296315 2314 94-95 2.140702972823579 2314 96-97 2.1051162344183503 2314 98-99 2.194583596759994 2314 100-101 2.057047524235232 2315 1 -0.46980523525497375 2315 2 -0.3812039468531587 2315 3 -0.3984495994503092 2315 4 -0.35418275547606015 2315 5 -0.45360413020714674 2315 6 -0.3409227641887256 2315 7 -0.3079693590757131 2315 8 -0.5546063389644971 2315 9 -0.576265216463355 2315 10-11 -0.40070634487288004 2315 12-13 -0.3191886316897481 2315 14-15 -0.5913927526127409 2315 16-17 -0.25614508298102123 2315 18-19 -0.22109535389223822 2315 20-21 -0.2079489195150046 2315 22-23 -0.35328756125387173 2315 24-25 -0.2058625867642192 2315 26-27 -0.29345287800999387 2315 28-29 -0.31823347916503764 2315 30-31 -0.32970196436556876 2315 32-33 -0.2836655142396083 2315 34-35 -0.3498363445888302 2315 36-37 -0.0711594357669938 2315 38-39 -0.09727777307740126 2315 40-41 -0.16927901232142517 2315 42-43 -0.0855138526530439 2315 44-45 0.005687603931683327 2315 46-47 0.1885250674528791 2315 48-49 0.16284898026997752 2315 50-51 0.46210613210401164 2315 52-53 0.7681765095731166 2315 54-55 0.6024294916370039 2315 56-57 0.7111868702297173 2315 58-59 0.9866220487988073 2315 60-61 1.022704180377346 2315 62-63 1.0201216402144553 2315 64-65 1.0350828972967854 2315 66-67 1.3375653462657482 2315 68-69 1.561455808704114 2315 70-71 1.5148724674182326 2315 72-73 1.5632257887382401 2315 74-75 1.8856308310943888 2315 76-77 1.9821344891767758 2315 78-79 1.9806230798461684 2315 80-81 2.2587043947324794 2315 82-83 2.451151172176896 2315 84-85 2.7589172176450028 2315 86-87 2.763407816458127 2315 88-89 3.072108433373362 2315 90-91 3.1123463172636505 2315 92-93 3.017997326082721 2315 94-95 3.1667490537729996 2315 96-97 3.2805628591268388 2315 98-99 3.078766513358179 2315 100-101 3.0620420082280706 2316 1 -0.9456534122283955 2316 2 -0.9783899731538135 2316 3 -0.7432503923189309 2316 4 -0.7237972968728812 2316 5 -0.6508546020405106 2316 6 -0.5144149078789653 2316 7 -0.5099667269027464 2316 8 -0.766560042447388 2316 9 -0.6536946938056545 2316 10-11 -0.663017410481423 2316 12-13 -0.4992756276128105 2316 14-15 -1.08794280909963 2316 16-17 -0.9286336622010509 2316 18-19 -0.9196677355983596 2316 20-21 -0.7293526563576123 2316 22-23 -0.8078426458233778 2316 24-25 -0.905058903274977 2316 26-27 -0.6792342634558466 2316 28-29 -0.5690436238458147 2316 30-31 -0.6702403119532718 2316 32-33 -0.713532746459272 2316 34-35 -0.5652624532377857 2316 36-37 -0.5896459307674817 2316 38-39 -0.8287270821181352 2316 40-41 -0.902896009525648 2316 42-43 -0.5494260181616042 2316 44-45 -0.4988998420563675 2316 46-47 -0.3548162171207352 2316 48-49 -0.2418428985391543 2316 50-51 -0.20316199625241183 2316 52-53 0.043628751384730435 2316 54-55 -0.23860245770725186 2316 56-57 -0.27767603215971093 2316 58-59 0.17823603225926377 2316 60-61 -0.07590469458223836 2316 62-63 -0.00425036247207089 2316 64-65 0.1707152316504157 2316 66-67 0.3509425084771536 2316 68-69 0.5196116903572694 2316 70-71 0.31702440469420523 2316 72-73 0.9094807656581985 2316 74-75 1.2300944218641483 2316 76-77 1.150517577382299 2316 78-79 1.2978487273934363 2316 80-81 1.632033150695218 2316 82-83 1.6901531427016572 2316 84-85 1.4052864994211696 2316 86-87 1.7923578367845359 2316 88-89 2.1033126608228336 2316 90-91 2.174627489462445 2316 92-93 2.3046526639420293 2316 94-95 2.505370950945217 2316 96-97 2.456302277331204 2316 98-99 2.7555910235138974 2316 100-101 2.742023905799604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3460.0 3 1206.0 4 1287.0 5 1385.0 6 1703.0 7 2117.0 8 2811.0 9 3634.0 10 4522.0 11 6124.0 12 7697.0 13 9572.0 14 11300.0 15 13939.0 16 16716.0 17 19933.0 18 17992.0 19 15308.0 20 15749.0 21 17041.0 22 18982.0 23 21206.0 24 23494.0 25 26382.0 26 29875.0 27 33514.0 28 37517.0 29 42264.0 30 48098.0 31 55145.0 32 63170.0 33 73552.0 34 87259.0 35 103842.0 36 122583.0 37 132839.0 38 82767.0 39 7133.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.886009396357764 19.279488235902495 14.832918788137734 22.001583579602002 2 14.760012818030471 20.60752053125494 42.110020300851694 22.5224463498629 3 19.213444667582706 25.851643589472573 29.824077793045134 25.110833949899586 4 12.225190281339872 17.044523423567405 37.96384379722152 32.7664424978712 5 12.893816326737731 38.51340151082033 34.155246430154165 14.43753573228778 6 30.476453853205733 36.82077121622673 18.361715227105893 14.341059703461646 7 28.024146502308124 33.48331896041529 20.800402441705135 17.69213209557145 8 26.027978673574815 35.8580538030603 20.001809330395567 18.112158192969314 9 27.15594980505957 16.72978660977839 20.57811610856461 35.53614747659742 10-11 24.57969178947938 27.348547426661128 27.659894670360842 20.411866113498647 12-13 26.183127463285604 24.88999103356642 28.1334641589963 20.793417344151674 14-15 22.2507938816161 26.729376832522263 26.88333406606042 24.136495219801215 16-17 20.898857881967047 29.651954112385763 28.177176237858127 21.27201176778906 18-19 21.04686714683726 27.375726374621834 31.300167773674204 20.277238704866704 20-21 22.147923675753006 26.63510587296259 30.960326915483417 20.256643535800983 22-23 21.466838185156266 27.148719297140566 30.831561713048668 20.552880804654503 24-25 21.176796914012737 26.96564458630883 31.452765798750367 20.40479270092807 26-27 20.52915229342593 27.51933543518417 31.11667978033305 20.834832491056847 28-29 20.56341422169612 27.671514331614105 30.834531157520928 20.93054028916885 30-31 20.81782327816721 27.293638583901842 30.96257251941637 20.925965618514578 32-33 20.314984929254155 27.61928717274499 31.063153400323824 21.002574497677035 34-35 20.900641926667753 27.682913167634382 30.986690114498412 20.429754791199453 36-37 20.79358389412681 27.32471601705652 30.86368607345362 21.018014015363043 38-39 20.867845796707428 27.75706607287477 30.548285324446745 20.826802805971056 40-41 21.509470009979555 27.114463084312234 30.761417629363308 20.614649276344903 42-43 20.72069216069429 27.60282000787046 30.786889389633735 20.889598441801517 44-45 20.768132529509423 27.966368895666317 30.326869042748513 20.938629532075755 46-47 21.25466953749705 27.787203501804413 29.694454377382318 21.263672583316215 48-49 21.18863464124087 27.96198134233572 29.47971821187029 21.369665804553126 50-51 21.21260276473701 28.464120462333543 29.158178661450734 21.165098111478713 52-53 21.32573671645096 28.24903503214728 29.348927219468322 21.076301031933433 54-55 20.93968002799565 28.081233025736218 29.612498636987223 21.366588309280914 56-57 21.12662305965049 28.33538943077164 29.021825907503935 21.516161602073932 58-59 21.242168035653535 28.110840389585416 29.007503893529663 21.63948768123139 60-61 20.96681691295647 27.917366653776337 29.68220056928763 21.43361586397956 62-63 21.105075488021193 27.905653814635418 29.640337816449343 21.348932880894047 64-65 20.93683856396108 28.035843493667116 29.33386417273582 21.693453769635987 66-67 20.900359185170753 28.424736992691624 29.02249144087814 21.652412381259488 68-69 21.35422918205778 27.81321373205685 29.446841278669734 21.385715807215636 70-71 21.315493497090586 27.83425284062451 29.14899399435573 21.701259667929165 72-73 21.228811320635092 28.061556798657616 28.988585956140145 21.72104592456714 74-75 20.967876910800566 28.28661078670377 29.227471910682223 21.51804039181344 76-77 21.14902226473133 27.79496785542358 29.40268272282377 21.65332715702132 78-79 21.137537223795842 27.97023279717543 29.086967804322228 21.8052621747065 80-81 21.26368988927213 28.208325109432476 28.927027126404916 21.600957874890483 82-83 21.148157305792772 28.25012204322549 29.04290638280786 21.558814268173872 84-85 21.14938661935491 28.001364242065907 29.10564803822583 21.74360110035336 86-87 21.05957359767663 28.25988836184017 28.73773991892541 21.942798121557793 88-89 21.363401770984314 27.9207947576362 28.793225809587696 21.922577661791788 90-91 21.31588147242573 28.064168265631494 28.89431952157451 21.72563074036827 92-93 21.338888790267976 27.951885955902927 28.956804885285564 21.752420368543536 94-95 21.221785577362436 27.81744778754628 28.940297163668273 22.02046947142301 96-97 21.71692472344719 27.498591399272048 28.675898408291744 22.108585468989013 98-99 21.63218244087709 27.42555773320017 28.63458019806751 22.307679627855233 100-101 21.97396476740054 27.1750461137529 28.970859492664108 21.880129626182455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 471.0 1 323.0 2 259.5 3 313.5 4 247.5 5 248.0 6 434.5 7 988.0 8 1526.5 9 1706.5 10 1552.5 11 1339.0 12 1426.0 13 1595.5 14 1752.5 15 1927.0 16 2116.5 17 2274.0 18 2443.5 19 2685.5 20 2977.0 21 3421.0 22 4005.5 23 4666.0 24 5411.5 25 6539.5 26 8058.5 27 9870.0 28 11830.0 29 14103.0 30 16979.5 31 20094.0 32 23853.0 33 27538.5 34 31120.0 35 35071.5 36 38287.5 37 40189.5 38 40804.0 39 40496.5 40 40320.0 41 41204.0 42 42028.5 43 42545.5 44 42798.5 45 42960.0 46 42557.0 47 42263.5 48 42829.0 49 42058.0 50 40264.5 51 37795.0 52 34992.5 53 32604.0 54 30849.5 55 28384.5 56 24741.0 57 21892.5 58 19683.0 59 16952.0 60 13811.0 61 11237.5 62 9169.0 63 7084.0 64 5236.5 65 3966.0 66 2965.0 67 2182.5 68 1641.5 69 1224.0 70 967.0 71 766.5 72 607.5 73 506.5 74 399.0 75 296.0 76 209.0 77 136.5 78 97.0 79 64.0 80 42.5 81 27.0 82 14.5 83 11.0 84 10.0 85 6.0 86 3.0 87 2.0 88 1.0 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2970955633447466 2 0.03482314995676689 3 0.04192301548193295 4 0.04479677057545255 5 0.06060242358981034 6 0.057221535244493166 7 0.028822073143828907 8 0.030681561733753353 9 0.03820403830208406 10-11 0.030258950690588704 12-13 0.0318226115502979 14-15 0.027469717805702037 16-17 0.019524630194206676 18-19 0.023454912895637895 20-21 0.03634454971215962 22-23 0.029794078543107592 24-25 0.05193889720493508 26-27 0.04331763192437629 28-29 0.03558384983446325 30-31 0.03554158873014679 32-33 0.03093512835965214 34-35 0.029878600751740526 36-37 0.02937146749994295 38-39 0.01817227485607981 40-41 0.01711574724816819 42-43 0.018299058169029204 44-45 0.009212920740989299 46-47 0.015890175222990716 48-49 0.014833647615079099 50-51 0.0066772544820014185 52-53 0.004268371535962932 54-55 0.006761776690634347 56-57 0.0046064603704946494 58-59 0.00578977129135566 60-61 0.005367160248191013 62-63 0.00443741595322879 64-65 0.007522476568330712 66-67 0.0022398385287726273 68-69 0.0066772544820014185 70-71 0.012720592399255866 72-73 0.012551547981990008 74-75 0.009212920740989299 76-77 0.009001615219406974 78-79 0.0062546434388367714 80-81 0.0072266488381154586 82-83 0.011706325895660714 84-85 0.0039302827014312145 86-87 0.0010987887122280815 88-89 0.01855262479492799 90-91 0.02742745670138557 92-93 0.01183310920861011 94-95 0.020074024550320722 96-97 0.017707402708598698 98-99 0.011875370312926573 100-101 0.10658250508612391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1183121.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.06336415668592 #Duplication Level Percentage of deduplicated Percentage of total 1 84.92726698984677 59.50290033941725 2 9.713672148344637 13.611450980562555 3 2.518780899628289 5.2942279020468535 4 0.9442544366155834 2.6463056979665565 5 0.5102666669117063 1.7875499650426618 6 0.3069895496158814 1.2905232364220696 7 0.20487644278445946 1.0048032972553806 8 0.14792103703212015 0.8291076387212845 9 0.09823964426102971 0.6194699973435424 >10 0.5694108227933689 7.256722190201661 >50 0.03659849967203973 1.7376879264852292 >100 0.020266585803064282 2.7250153778809705 >500 9.708511274101269E-4 0.40311002065396595 >1k 3.640691727787976E-4 0.7886195826968012 >5k 1.2135639092626587E-4 0.5025058473032986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5910 0.49952625302061243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4765 0.4027483241359083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3216 0.27182342296350076 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.10937173797101057 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.452220863292935E-5 0.0 0.0 0.0 0.0 8 8.452220863292935E-5 0.0 0.0 0.0 0.0 9 8.452220863292935E-5 0.0 0.0 0.0 0.0 10-11 8.452220863292935E-5 0.0 0.0 0.0 0.0 12-13 8.452220863292935E-5 0.0 0.0 0.0 0.0 14-15 3.380888345317174E-4 0.0 0.0 0.0 0.0 16-17 3.380888345317174E-4 0.0 0.0 8.452220863292935E-5 0.0 18-19 3.8034993884818207E-4 0.0 0.0 1.690444172658587E-4 0.0 20-21 4.2261104316464675E-4 0.0 0.0 2.5356662589878803E-4 0.0 22-23 5.071332517975761E-4 0.0 0.0 6.761776690634348E-4 0.0 24-25 5.916554604305054E-4 0.0 0.0 9.297442949622228E-4 0.0 26-27 5.916554604305054E-4 0.0 0.0 0.0019862719028738395 0.0 28-29 0.0010142665035951521 0.0 0.0 0.004352893744595862 0.0 30-31 0.0010987887122280815 0.0 0.0 0.01356581448558516 0.0 32-33 0.0010987887122280815 0.0 0.0 0.03173808934166497 0.0 34-35 0.0012255720251774754 0.0 0.0 0.06153216788477256 0.0 36-37 0.001436877546759799 0.0 0.0 0.1162602979745943 0.0 38-39 0.001436877546759799 0.0 0.0 0.19592247961113024 0.0 40-41 0.0015636608597091929 0.0 0.0 0.28403688211095907 0.0 42-43 0.0022398385287726278 0.0 0.0 0.3722358068194208 0.0 44-45 0.002451144050354951 0.0 0.0 0.4837628611105711 0.0 46-47 0.002831493989203133 0.0 0.0 0.611898529398092 0.0 48-49 0.002873755093519598 0.0 0.0 0.7521631346244382 0.0 50-51 0.002873755093519598 0.0 0.0 0.9043876323723441 0.0 52-53 0.002873755093519598 0.0 0.0 1.0517943642281729 0.0 54-55 0.002873755093519598 0.0 0.0 1.2168239765839672 0.0 56-57 0.0030427995107854566 0.0 0.0 1.4067876404864759 0.0 58-59 0.0030427995107854566 0.0 0.0 1.6208401338493696 0.0 60-61 0.0030427995107854566 0.0 0.0 1.8521774188776972 0.0 62-63 0.0030427995107854566 0.0 0.0 2.0808522543340873 0.0 64-65 0.0030427995107854566 0.0 0.0 2.31954297151348 0.0 66-67 0.0031273217194183857 0.0 0.0 2.5986353044194126 0.0 68-69 0.0031273217194183857 0.0 0.0 2.9170304643396574 0.0 70-71 0.0032118439280513153 0.0 0.0 3.257950792860578 0.0 72-73 0.0033386272410007092 0.0 0.0 3.589784983953459 0.0 74-75 0.003423149449633639 0.0 0.0 3.9194638587261994 0.0 76-77 0.0035076716582665675 0.0 0.0 4.277838023329821 0.0 78-79 0.0035499327625830323 0.0 0.0 4.678346508936956 0.0 80-81 0.004141588223013538 0.0 0.0 5.1050568792202995 0.0 82-83 0.004310632640279396 0.0 0.0 5.55129187969785 0.0 84-85 0.00452193816186172 0.0 0.0 5.985566987653842 0.0 86-87 0.004648721474811114 0.0 0.0 6.4292663218724035 0.0 88-89 0.004648721474811114 0.0 0.0 6.904915050954214 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3645 0.0 52.16462 1 GTATCAA 6225 0.0 39.348164 1 GTCCTAC 1095 0.0 30.899036 1 ATCAACG 7910 0.0 30.819641 3 TATCAAC 7990 0.0 30.570538 2 TCAACGC 8090 0.0 30.133915 4 CAACGCA 8275 0.0 29.460224 5 AACGCAG 8490 0.0 28.818811 6 TAGCCCT 875 0.0 25.525633 4 GTACATG 7380 0.0 25.312231 1 ACGCAGA 9730 0.0 25.189617 7 CGCAGAG 9810 0.0 24.887362 8 TACATGG 7430 0.0 24.879835 2 ATGGGCG 195 1.5625119E-8 24.36981 5 ACATGGG 7375 0.0 23.905542 3 GAGTACT 5480 0.0 23.706676 12-13 GCAGAGT 10020 0.0 23.6073 9 TCCTACA 1655 0.0 22.970938 2 GTACTTT 5860 0.0 21.88568 14-15 CATGGGG 3450 0.0 21.625555 4 >>END_MODULE