FastQCFastQC Report
Thu 26 May 2016
SRR938641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183121
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57210.4835515555889888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.41263742254596103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.2817125213735535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.11790848104293644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32600.054.1234781
GTATCAA60000.042.96131
TATCAAC78100.032.7003672
ATCAACG78200.032.58673
TCAACGC80100.031.8137324
CAACGCA81600.031.2289245
AACGCAG83700.030.5847516
ACGCAGA95950.026.5809767
GTACATG74500.026.556231
CGCAGAG96800.026.2985068
TACATGG74300.026.243662
ACATGGG74000.025.120453
GCAGAGT103100.024.3690539
TGGTATC15250.024.3250712
GTGTAGC6700.024.1342321
GTGGTAT16200.022.6050281
GAGTACT60900.022.10942512-13
AGTACTT61650.021.87897112-13
GTACTTT63650.021.04225714-15
GTAGGAC14400.020.799863
Produced by FastQC (version 0.11.5)