##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938641_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1183121 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787718246907968 31.0 31.0 34.0 30.0 34.0 2 32.01717998412673 31.0 31.0 34.0 30.0 34.0 3 32.07707749249654 33.0 31.0 34.0 30.0 34.0 4 35.74797421396459 37.0 35.0 37.0 33.0 37.0 5 35.62673302223526 37.0 35.0 37.0 33.0 37.0 6 35.679928764682565 37.0 35.0 37.0 33.0 37.0 7 35.61658359542262 37.0 35.0 37.0 33.0 37.0 8 35.608556521268746 37.0 35.0 37.0 33.0 37.0 9 37.1648377469422 39.0 37.0 39.0 33.0 39.0 10-11 37.09379894364143 39.0 37.0 39.0 33.0 39.0 12-13 36.98656139143841 39.0 37.0 39.0 33.0 39.0 14-15 37.99699608070519 40.0 37.0 41.0 33.0 41.0 16-17 37.940893619502994 40.0 37.0 41.0 33.0 41.0 18-19 37.985894088601256 40.0 37.0 41.0 33.0 41.0 20-21 38.01389418326612 40.0 37.0 41.0 33.0 41.0 22-23 38.03377211629241 40.0 37.0 41.0 33.0 41.0 24-25 37.96451039242817 40.0 37.0 41.0 33.0 41.0 26-27 37.79634754179834 40.0 37.0 41.0 32.0 41.0 28-29 37.660997480392965 40.0 37.0 41.0 32.0 41.0 30-31 37.40932626502277 39.0 36.0 41.0 32.0 41.0 32-33 37.16875619653442 39.0 36.0 41.0 31.0 41.0 34-35 36.98530454619603 39.0 35.5 41.0 31.0 41.0 36-37 36.958029229470185 39.0 35.5 41.0 31.0 41.0 38-39 36.80767858908767 39.0 35.0 41.0 30.5 41.0 40-41 36.606195393370584 39.0 35.0 41.0 30.0 41.0 42-43 36.36700557254922 39.0 35.0 40.0 30.0 41.0 44-45 36.0302365523053 39.0 35.0 40.0 29.5 41.0 46-47 35.57658176974291 38.0 35.0 40.0 27.5 41.0 48-49 35.123056306159725 38.0 34.5 40.0 26.5 41.0 50-51 34.758002351407846 38.0 34.0 40.0 24.5 41.0 52-53 34.2707787284648 38.0 33.0 40.0 24.0 41.0 54-55 33.97974721097842 38.0 33.0 40.0 23.0 41.0 56-57 33.64471765778817 37.5 33.0 40.0 22.0 41.0 58-59 33.224862883847045 37.0 32.0 40.0 20.5 41.0 60-61 32.858801001757215 36.5 31.5 39.0 19.0 41.0 62-63 32.40632403617212 36.0 31.0 39.0 17.5 40.0 64-65 31.94011094385105 35.5 31.0 39.0 13.5 40.0 66-67 31.45876795357364 35.0 30.0 38.5 10.0 40.0 68-69 30.91874161645343 35.0 29.0 38.0 8.0 40.0 70-71 30.366083012641987 34.0 29.0 37.5 7.0 39.5 72-73 29.806957614648034 34.0 28.0 37.0 3.5 39.0 74-75 29.193732931796497 34.0 27.5 36.0 2.0 39.0 76-77 27.89246154873424 31.5 26.0 35.0 2.0 37.0 78-79 28.520450993600825 33.0 27.0 35.0 2.0 37.5 80-81 28.20599710426913 33.0 26.0 35.0 2.0 37.0 82-83 27.70521949994971 33.0 26.0 35.0 2.0 37.0 84-85 27.107596348978674 33.0 25.5 35.0 2.0 36.0 86-87 26.442396424372486 32.0 24.5 35.0 2.0 36.0 88-89 25.685609924935825 32.0 22.0 34.0 2.0 35.0 90-91 25.13254645974503 31.0 19.5 34.0 2.0 35.0 92-93 24.362074969508612 31.0 16.0 34.0 2.0 35.0 94-95 23.58302954642847 31.0 4.5 34.0 2.0 35.0 96-97 22.5825334010638 30.0 2.0 34.0 2.0 35.0 98-99 21.404146321466698 29.5 2.0 34.0 2.0 35.0 100-101 18.313986904128996 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38232982515011926 1101 2 0.31518544063873577 1101 3 0.3320802307735846 1101 4 0.3455671611548681 1101 5 0.19984141217737061 1101 6 0.25334096634425407 1101 7 0.35575947176128864 1101 8 0.37909133098447967 1101 9 0.48388044133832864 1101 10-11 0.351398762421276 1101 12-13 0.46453201893601914 1101 14-15 0.6979955666553437 1101 16-17 0.7221444325909445 1101 18-19 0.6404666979045572 1101 20-21 0.6952248024053702 1101 22-23 0.6384867621483963 1101 24-25 0.5962303694878344 1101 26-27 0.4763928872082275 1101 28-29 0.43067922968828043 1101 30-31 0.5792619315637921 1101 32-33 0.6327806002025795 1101 34-35 0.533635581057986 1101 36-37 0.659856490533997 1101 38-39 0.8202986631553131 1101 40-41 0.9699486880979933 1101 42-43 0.9974426041804278 1101 44-45 0.8850362423584812 1101 46-47 0.7841647886127987 1101 48-49 0.9277261796593876 1101 50-51 0.9760382445970777 1101 52-53 1.1420354492417886 1101 54-55 1.1937524947327844 1101 56-57 1.1757037706913138 1101 58-59 1.1806959408800353 1101 60-61 1.214465675817614 1101 62-63 1.3854602289979283 1101 64-65 1.5826670524809288 1101 66-67 1.5877847993756546 1101 68-69 1.5888118417491484 1101 70-71 1.6060194467352495 1101 72-73 1.6367757137997252 1101 74-75 1.581725385517835 1101 76-77 1.6053906931915307 1101 78-79 1.8512966819928138 1101 80-81 2.208411271885371 1101 82-83 2.312024020424083 1101 84-85 2.595951359541477 1101 86-87 2.6254441796172117 1101 88-89 2.757596577891821 1101 90-91 3.0843082310287357 1101 92-93 3.3361039114028372 1101 94-95 3.151872481909198 1101 96-97 3.4849749977272104 1101 98-99 3.4661875249844414 1101 100-101 4.00761895474777 1102 1 0.42188985842491533 1102 2 0.31018189748934333 1102 3 0.34217974705666165 1102 4 0.21687645388942656 1102 5 0.19371374436192923 1102 6 0.1862570443199516 1102 7 0.26241927220055317 1102 8 0.17981228486191725 1102 9 0.41168382075461096 1102 10-11 0.2618864846383602 1102 12-13 0.3950193890388718 1102 14-15 0.4378082418871827 1102 16-17 0.5939105935335576 1102 18-19 0.5588417787807884 1102 20-21 0.421314705970083 1102 22-23 0.49114324391108966 1102 24-25 0.5145183020931725 1102 26-27 0.47865698168048 1102 28-29 0.4622159001841908 1102 30-31 0.6320156848813667 1102 32-33 0.3114288336895328 1102 34-35 0.3955649909585617 1102 36-37 0.5256208644969007 1102 38-39 0.6373264669748693 1102 40-41 0.7167002142930912 1102 42-43 0.6808364571463272 1102 44-45 0.6636580672784831 1102 46-47 0.6345386899063854 1102 48-49 0.8325831552622773 1102 50-51 0.6525368207351718 1102 52-53 0.9439254223490607 1102 54-55 0.8611405554196736 1102 56-57 0.8873160577172285 1102 58-59 1.0110979232831312 1102 60-61 0.9324543113309716 1102 62-63 1.091104179895602 1102 64-65 1.0210290171022507 1102 66-67 0.9497771496940999 1102 68-69 1.1333393264651903 1102 70-71 1.4036501581380278 1102 72-73 1.241727760243588 1102 74-75 1.3377837308450573 1102 76-77 1.226382422908653 1102 78-79 1.6602478657568227 1102 80-81 2.0058861727432102 1102 82-83 1.8794926272401327 1102 84-85 2.107229578195607 1102 86-87 2.2670706832549925 1102 88-89 2.4541775487587714 1102 90-91 2.6041674733669495 1102 92-93 3.016984108938921 1102 94-95 3.0339696742383886 1102 96-97 3.4980542806465884 1102 98-99 3.9638706133783153 1102 100-101 4.300011967040955 1103 1 0.2834355451195627 1103 2 0.31575294448220603 1103 3 0.3193848308571887 1103 4 0.20456027202321536 1103 5 0.21957890036885885 1103 6 0.14872732522047727 1103 7 0.2511128059115464 1103 8 0.2832787793290308 1103 9 0.31220922508112636 1103 10-11 0.3804135913923048 1103 12-13 0.4187271585654244 1103 14-15 0.5959226567511351 1103 16-17 0.582113945554454 1103 18-19 0.4867557181391504 1103 20-21 0.38549328473670386 1103 22-23 0.45870319577348795 1103 24-25 0.41895928950108186 1103 26-27 0.5565312955728743 1103 28-29 0.4230718102490556 1103 30-31 0.6024257350736235 1103 32-33 0.6538603696899941 1103 34-35 0.761763000879597 1103 36-37 0.9165833230369316 1103 38-39 0.9197122135480029 1103 40-41 0.7495981567040886 1103 42-43 0.9798208711297107 1103 44-45 1.0107448617250512 1103 46-47 1.0641345101535222 1103 48-49 1.038353797194823 1103 50-51 1.2079009347391079 1103 52-53 1.4912675809096356 1103 54-55 1.445369619965156 1103 56-57 1.31763100679116 1103 58-59 1.5450290630289416 1103 60-61 1.483224806502534 1103 62-63 1.6707777834648283 1103 64-65 1.7771592234481943 1103 66-67 1.783766993833936 1103 68-69 1.834663110550423 1103 70-71 1.961829147835406 1103 72-73 1.8886648584853951 1103 74-75 1.8854898566485758 1103 76-77 1.6792222479695589 1103 78-79 2.0609376423866053 1103 80-81 2.182850568528238 1103 82-83 2.28840395425296 1103 84-85 2.457771126230476 1103 86-87 2.535543488444464 1103 88-89 2.690624171788503 1103 90-91 2.9661974713122987 1103 92-93 3.2852984122645204 1103 94-95 3.169206986751231 1103 96-97 3.2569670752692694 1103 98-99 3.4519947144672827 1103 100-101 3.873688916760642 1104 1 0.3630492955014155 1104 2 0.3935721795302243 1104 3 0.3030994204220434 1104 4 0.21863786500394156 1104 5 0.29427468223681785 1104 6 0.29634234327998143 1104 7 0.32728536689811705 1104 8 0.3168077096606652 1104 9 0.34324609065011913 1104 10-11 0.41552058436616335 1104 12-13 0.45625883008661816 1104 14-15 0.5976738815466476 1104 16-17 0.6599229705698519 1104 18-19 0.6140511376734779 1104 20-21 0.6267093894704061 1104 22-23 0.4037556590051068 1104 24-25 0.6446438926085207 1104 26-27 0.6477665134876389 1104 28-29 0.6608847069088313 1104 30-31 0.8509460971458793 1104 32-33 0.7123736582516571 1104 34-35 0.8408135658619642 1104 36-37 0.7867047652489632 1104 38-39 0.8762926204137216 1104 40-41 1.0119996707294021 1104 42-43 1.0004974123953971 1104 44-45 0.9591507080714123 1104 46-47 0.9568103228312879 1104 48-49 1.1218955513274338 1104 50-51 1.1397456997694988 1104 52-53 1.2700566056963254 1104 54-55 1.3550790342686767 1104 56-57 1.4710277621244785 1104 58-59 1.6532125266967412 1104 60-61 1.6202977616780458 1104 62-63 1.6625505391771398 1104 64-65 1.8122976633225392 1104 66-67 1.8004497787387734 1104 68-69 2.06190372262963 1104 70-71 1.9768562738500854 1104 72-73 2.0804705313428826 1104 74-75 2.1975517441487185 1104 76-77 1.9433263870351034 1104 78-79 2.335734136016363 1104 80-81 2.6438723395849983 1104 82-83 2.8163618823841325 1104 84-85 3.1235869568452195 1104 86-87 3.2296570370760698 1104 88-89 3.1938730041691734 1104 90-91 3.5394879442900162 1104 92-93 3.6137682305912833 1104 94-95 4.194725533888093 1104 96-97 4.377722764707844 1104 98-99 4.756926731986066 1104 100-101 4.741449391028912 1105 1 0.3685572744078307 1105 2 0.3102268564642756 1105 3 0.31932934804576263 1105 4 0.0950039125833726 1105 5 0.2058358079788789 1105 6 0.16351904274638684 1105 7 0.14489651171948736 1105 8 0.30106102039074756 1105 9 0.21529739836504547 1105 10-11 0.27358143799342116 1105 12-13 0.43900612614127255 1105 14-15 0.5405563592301093 1105 16-17 0.6230130999964061 1105 18-19 0.586061628168224 1105 20-21 0.5174341844459747 1105 22-23 0.31350190413363777 1105 24-25 0.1982403432306299 1105 26-27 0.5604317372160494 1105 28-29 0.577993690450576 1105 30-31 0.5679154263672643 1105 32-33 0.6030186954406815 1105 34-35 0.6913247976349624 1105 36-37 0.8420449400279821 1105 38-39 1.0066981653001505 1105 40-41 0.9538054182944435 1105 42-43 1.0744579025773717 1105 44-45 1.0009993058982403 1105 46-47 1.171834969551206 1105 48-49 1.16763429609626 1105 50-51 1.2899960766641385 1105 52-53 1.4198667508867757 1105 54-55 1.3986447703235783 1105 56-57 1.5716562847600528 1105 58-59 1.6172011041306078 1105 60-61 1.5877988094764106 1105 62-63 1.7818932099057179 1105 64-65 1.677337323272873 1105 66-67 1.7376663258208893 1105 68-69 1.9276744956233358 1105 70-71 2.0260144382324405 1105 72-73 1.974025163862791 1105 74-75 2.0247877771528024 1105 76-77 1.8858069480294795 1105 78-79 2.539482439208548 1105 80-81 2.643235841170771 1105 82-83 2.6549899859003574 1105 84-85 2.8163787295949554 1105 86-87 2.9554263087467305 1105 88-89 3.0509517423071557 1105 90-91 3.198173880020878 1105 92-93 3.2965558747026584 1105 94-95 3.6352903441271174 1105 96-97 3.9359490766452403 1105 98-99 4.299905231789019 1105 100-101 4.854993983450981 1106 1 0.2141091578849732 1106 2 0.18840424277523482 1106 3 0.19232511601693147 1106 4 -0.05723995994732434 1106 5 0.09488262947100878 1106 6 0.2060232672981357 1106 7 0.05898184018773378 1106 8 0.07894007110032675 1106 9 0.060132229607283705 1106 10-11 0.18554701350448255 1106 12-13 0.14170161446953955 1106 14-15 0.18406326057748146 1106 16-17 0.023391814237257336 1106 18-19 0.2188168583876049 1106 20-21 0.41583221946863347 1106 22-23 0.28751792004486987 1106 24-25 0.29682680073836565 1106 26-27 0.12125107594285112 1106 28-29 0.2033839941696769 1106 30-31 0.23044926016666523 1106 32-33 0.26897516386495823 1106 34-35 0.251182957375363 1106 36-37 0.5062719592141747 1106 38-39 0.4136817605806158 1106 40-41 0.3989434452418479 1106 42-43 0.318832145522741 1106 44-45 -0.12298381084892895 1106 46-47 -0.4360888688839921 1106 48-49 0.09036088908873552 1106 50-51 0.04337758733394281 1106 52-53 0.2839819876859835 1106 54-55 0.19329379605969166 1106 56-57 0.08071215352229899 1106 58-59 0.7160780463028473 1106 60-61 0.5548787095825887 1106 62-63 0.6692098344514861 1106 64-65 0.38534340961822977 1106 66-67 0.3649449667863287 1106 68-69 0.33710309696023444 1106 70-71 0.7344960670401477 1106 72-73 1.1866517134881853 1106 74-75 1.152273159456044 1106 76-77 1.2810530188225009 1106 78-79 1.5169397824515087 1106 80-81 1.4096697213280862 1106 82-83 1.9089520920450234 1106 84-85 1.8973959119310955 1106 86-87 2.020236166532616 1106 88-89 2.298284666893302 1106 90-91 2.309236597790914 1106 92-93 1.8697856753636302 1106 94-95 2.342925193314361 1106 96-97 3.0258049184899853 1106 98-99 3.213157613239872 1106 100-101 3.8215127284974137 1107 1 0.2627500734786814 1107 2 0.21301431291026773 1107 3 0.19691355245923603 1107 4 0.15412121804214252 1107 5 0.1263352272587852 1107 6 0.18396039001700615 1107 7 0.23095922948952818 1107 8 0.19368587521072556 1107 9 0.27656336816760074 1107 10-11 0.3523270825927298 1107 12-13 0.34916872776728525 1107 14-15 0.48329735806866836 1107 16-17 0.5249585123076699 1107 18-19 0.4543833470997001 1107 20-21 0.4111914434471373 1107 22-23 0.4824043431580236 1107 24-25 0.31191630141878335 1107 26-27 0.46478713791291426 1107 28-29 0.4809843989290883 1107 30-31 0.49497698269711066 1107 32-33 0.41204889288550817 1107 34-35 0.5000646350333398 1107 36-37 0.4835373906667755 1107 38-39 0.48288864149062505 1107 40-41 0.627121330024984 1107 42-43 0.5743417585587807 1107 44-45 0.5343036032269382 1107 46-47 0.6183216129890639 1107 48-49 0.6947539244354033 1107 50-51 0.6474583298581038 1107 52-53 0.644779642872841 1107 54-55 0.8040164311830367 1107 56-57 0.6676202801142921 1107 58-59 0.518885192400063 1107 60-61 0.7005746515205544 1107 62-63 0.9008246849347543 1107 64-65 0.7404325613681095 1107 66-67 0.9568881608963835 1107 68-69 1.1342366326732787 1107 70-71 1.1424778528665875 1107 72-73 1.1842458374980964 1107 74-75 1.4393348154803292 1107 76-77 1.3205920815950982 1107 78-79 1.433384878232939 1107 80-81 1.536847920148702 1107 82-83 1.652957465575156 1107 84-85 1.8459373823243403 1107 86-87 2.128132464379714 1107 88-89 2.272554529763603 1107 90-91 2.2563910693356277 1107 92-93 2.52762439967362 1107 94-95 2.5245470224081856 1107 96-97 2.9218375783874 1107 98-99 3.203970272439424 1107 100-101 3.784407456157137 1108 1 0.1650077096008573 1108 2 0.21358762940040776 1108 3 0.16091769847678705 1108 4 0.11459801006105863 1108 5 0.10324061384253014 1108 6 0.0829174074756267 1108 7 0.21231186771710497 1108 8 0.16220529347263835 1108 9 0.2538671611258465 1108 10-11 0.12115907170942819 1108 12-13 0.2359597749606266 1108 14-15 0.4073879874211812 1108 16-17 0.4412089576806508 1108 18-19 0.36898186360279794 1108 20-21 0.3939660361332997 1108 22-23 0.34260743323672926 1108 24-25 0.34301629170155934 1108 26-27 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62-63 0.25650772887664175 2316 64-65 0.3624121344617919 2316 66-67 0.411698099443079 2316 68-69 0.4105857392272654 2316 70-71 0.37579670121807496 2316 72-73 0.46815610457650436 2316 74-75 0.5725066566756141 2316 76-77 0.6115771836371771 2316 78-79 0.7955661315845184 2316 80-81 0.8521563886238326 2316 82-83 1.1487491160617864 2316 84-85 1.4042277229987867 2316 86-87 1.452821653652201 2316 88-89 1.4646309365018908 2316 90-91 1.7038537319834859 2316 92-93 1.4409154491876066 2316 94-95 2.1676444662711774 2316 96-97 2.5214252186914017 2316 98-99 2.9671697891957756 2316 100-101 3.151967260640699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 19.0 5 45.0 6 82.0 7 230.0 8 523.0 9 905.0 10 1559.0 11 2611.0 12 3745.0 13 5416.0 14 6921.0 15 9725.0 16 12537.0 17 16448.0 18 15092.0 19 10956.0 20 10332.0 21 11024.0 22 12121.0 23 13755.0 24 15911.0 25 18658.0 26 21862.0 27 26187.0 28 31433.0 29 36900.0 30 44295.0 31 52167.0 32 62034.0 33 75489.0 34 93446.0 35 118175.0 36 151189.0 37 180232.0 38 113483.0 39 7604.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25835080127121 20.159493542497803 15.493694637906552 22.08846101832443 2 15.063889807311623 21.40145765667188 41.00219604036249 22.532456495654003 3 19.174334259135605 26.041358511999647 30.322926369136464 24.461380859728276 4 12.600655385205739 17.753805401138177 37.47309024182649 32.172448971829596 5 12.710536424726406 38.837190805694746 34.2787905863798 14.173482183199054 6 30.49535930813501 37.1717685680501 18.013204059432635 14.319668064382258 7 27.766390758003617 33.639416424862716 20.802775033153836 17.791417783979828 8 25.91222706722305 36.448342984360856 19.77938013102633 17.86004981738977 9 26.526365435149913 16.836739437470893 20.58910288972979 36.0477922376494 10-11 24.444076303269068 27.629507041122594 27.622195870075846 20.304220785532504 12-13 26.02181011071564 24.989244548951458 28.134104626661177 20.85484071367172 14-15 21.966054190568844 26.92281685474267 26.89961550847293 24.21151344621556 16-17 20.6956431337116 29.817491194898913 28.221162501553092 21.26570316983639 18-19 20.835189300164565 27.53053153481343 31.35888045263333 20.27539871238867 20-21 21.95578474221994 26.67964646050573 31.088198079486375 20.27637071778795 22-23 21.380526590264225 27.29932103309805 30.83053212646889 20.489620250168834 24-25 21.08545549979841 27.14336065968674 31.400203024747555 20.370980815767297 26-27 20.345992827390326 27.735640515734726 31.179761152520452 20.738605504354496 28-29 20.38316452839566 27.792888470410045 30.90584141436083 20.91810558683347 30-31 20.72573303998492 27.332622783299428 31.086761201939616 20.854882974776036 32-33 20.294806381963554 27.711326578005274 31.0719029545298 20.921964085501372 34-35 20.82369629464316 27.816255486349217 30.979740239539588 20.380307979468036 36-37 20.72827426950484 27.377207629717066 30.913725942652448 20.980792158125645 38-39 20.765273991419935 27.753333755996536 30.732686658636066 20.748705593947463 40-41 21.428197089355617 27.211067785707467 30.83973704964426 20.520998075292656 42-43 20.670015886748068 27.73253968622933 30.736356478509357 20.861087948513244 44-45 20.682187879963536 28.045868004180573 30.3459879020244 20.925956213831487 46-47 21.13229379425004 27.796619194748125 29.800557086112526 21.27052992488931 48-49 21.168967502056002 27.934040558827032 29.569587556978533 21.32740438213843 50-51 21.110520394786334 28.508622533113687 29.227695223058337 21.153161849041645 52-53 21.24444583436521 28.341268560020488 29.269364671914367 21.144920933699936 54-55 20.89706838821574 28.155754211003863 29.52615561982064 21.42102178095976 56-57 21.05672200899147 28.388770041272192 28.933219848181206 21.621288101555123 58-59 21.134736007559667 28.18587447944885 28.943911907573273 21.73547760541821 60-61 20.851966958578203 27.968694664366534 29.640205862291346 21.539132514763917 62-63 21.039944350577837 28.033988070535475 29.4930104359571 21.43305714292959 64-65 20.85868647416452 28.10722656431591 29.16903681026708 21.865050151252493 66-67 20.722828836237884 28.489906522228807 28.87938667019408 21.907877971339232 68-69 21.17434609227482 27.838009395512536 29.344368017391332 21.64327649482131 70-71 21.066780151818794 27.965313776021215 29.015629001598313 21.952277070561678 72-73 21.066737890714474 28.172181881650317 28.776896023314606 21.984184204320606 74-75 20.788575302103503 28.43783518338361 28.907736402278385 21.865853112234504 76-77 20.817017025308484 27.879354689841527 29.405360905604756 21.898267379245233 78-79 20.68199279701738 28.00563086953913 29.154921601425382 22.15745473201811 80-81 20.75460614535882 28.287524389950136 28.944219967450486 22.013649497240557 82-83 20.72909843166921 28.253705292429665 29.06804552427722 21.949150751623904 84-85 20.62035312057289 28.02345120924524 29.127460162359725 22.228735507822144 86-87 20.489866849463738 28.230271905008635 28.835716767095178 22.444144478432452 88-89 20.641270538914057 27.966351680302925 28.805826965988235 22.58655081479478 90-91 20.51601506206127 28.092772830813832 29.031573698023422 22.359638409101475 92-93 20.382439092008266 28.018500291391423 29.051788222039 22.54727239456131 94-95 20.189481887313303 27.902851863841484 29.02310921706233 22.884557031782887 96-97 20.594554572186617 27.605375950557885 28.873758474407946 22.92631100284755 98-99 20.408647974298486 27.54536518242851 28.8618408429907 23.184146000282304 100-101 20.164523661105772 27.4658158036535 29.26023518473518 23.109425350505553 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 559.0 1 358.0 2 222.5 3 305.5 4 257.0 5 245.5 6 442.0 7 968.5 8 1473.0 9 1658.0 10 1526.0 11 1325.0 12 1393.0 13 1559.0 14 1721.0 15 1876.5 16 2047.5 17 2204.0 18 2428.5 19 2700.5 20 3028.5 21 3419.5 22 3899.0 23 4575.0 24 5400.5 25 6486.0 26 8005.0 27 9915.5 28 11776.0 29 14062.5 30 16941.5 31 19960.0 32 23927.0 33 27759.5 34 31375.5 35 35435.0 36 38646.5 37 40617.5 38 41366.5 39 40794.0 40 40678.5 41 41631.0 42 42583.0 43 43254.5 44 43131.0 45 43640.0 46 43294.5 47 42686.0 48 43366.5 49 42343.5 50 40602.5 51 38177.5 52 34718.5 53 32365.0 54 31028.0 55 28569.5 56 24820.5 57 21539.5 58 18947.5 59 16123.5 60 13079.5 61 10652.0 62 8639.0 63 6480.0 64 4633.5 65 3455.0 66 2520.0 67 1889.5 68 1426.5 69 1032.5 70 783.0 71 613.0 72 523.0 73 464.0 74 368.5 75 260.5 76 160.5 77 97.5 78 69.0 79 42.5 80 20.5 81 12.5 82 7.5 83 3.5 84 1.0 85 2.5 86 2.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.452220863292935E-5 2 0.04547294824451599 3 2.5356662589878803E-4 4 0.0 5 0.10641346066885804 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011833109208610108 26-27 0.012551547981990008 28-29 0.0 30-31 0.0 32-33 4.6487214748111143E-4 34-35 0.015636608597091928 36-37 0.0030850606151019214 38-39 0.012340242460407685 40-41 0.0070998655251660655 42-43 0.0050290714136592965 44-45 0.002577927363304345 46-47 4.2261104316464674E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.2261104316464674E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.6487214748111143E-4 68-69 2.5356662589878803E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.2261104316464674E-5 82-83 0.0013523553381268696 84-85 0.005832032395672124 86-87 0.0020285330071903042 88-89 8.452220863292935E-5 90-91 0.0013523553381268696 92-93 3.8034993884818207E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.184387733798994E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1183121.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.42671957925907 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93772009251961 57.43582834893181 2 10.297390995067838 14.092333720349892 3 2.7224272761958623 5.588603034095411 4 1.0471413145494306 2.8660978036212246 5 0.5252424688430016 1.7970309563318898 6 0.3065414998192772 1.2585377548523509 7 0.20707714849951936 0.9918726980154441 8 0.15929149267836262 0.8719835440691135 9 0.10411275814722955 0.6411685055727327 >10 0.6266175036407773 7.79974952881549 >50 0.04254162394319796 1.98080376523944 >100 0.02203864344396856 2.822896415244196 >500 0.001361933944752257 0.5542422372990062 >1k 3.714365303869792E-4 0.8141650719112581 >5k 1.238121767956597E-4 0.48468661565081234 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5721 0.4835515555889888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4882 0.41263742254596103 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.2817125213735535 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1395 0.11790848104293644 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.452220863292935E-5 0.0 0.0 0.0 0.0 2 1.690444172658587E-4 0.0 0.0 0.0 0.0 3 1.690444172658587E-4 0.0 0.0 0.0 0.0 4 1.690444172658587E-4 0.0 0.0 0.0 0.0 5 2.5356662589878803E-4 0.0 0.0 0.0 0.0 6 2.5356662589878803E-4 0.0 0.0 0.0 0.0 7 3.380888345317174E-4 0.0 0.0 0.0 0.0 8 4.2261104316464675E-4 0.0 0.0 0.0 0.0 9 4.2261104316464675E-4 0.0 0.0 0.0 0.0 10-11 4.2261104316464675E-4 0.0 0.0 0.0 0.0 12-13 4.2261104316464675E-4 0.0 0.0 0.0 0.0 14-15 6.761776690634348E-4 0.0 0.0 0.0 0.0 16-17 8.452220863292934E-4 0.0 0.0 4.2261104316464674E-5 0.0 18-19 0.0010565276079116167 0.0 0.0 8.452220863292935E-5 0.0 20-21 0.0012255720251774754 0.0 0.0 1.690444172658587E-4 0.0 22-23 0.0012678331294939402 0.0 0.0 6.339165647469701E-4 0.0 24-25 0.0016059219640256577 0.0 0.0 9.297442949622228E-4 0.0 26-27 0.0016059219640256577 0.0 0.0 0.0020285330071903042 0.0 28-29 0.002197577424456163 0.0 0.0 0.004352893744595862 0.0 30-31 0.0023666218417220217 0.0 0.0 0.013312247859686373 0.0 32-33 0.0023666218417220217 0.0 0.0 0.031695828237348504 0.0 34-35 0.0025356662589878804 0.0 0.0 0.06161669009340549 0.0 36-37 0.0027892328848866683 0.0 0.0 0.1166406479134425 0.0 38-39 0.002916016197836062 0.0 0.0 0.19664091838451014 0.0 40-41 0.002958277302152527 0.0 0.0 0.2851779319275036 0.0 42-43 0.0032118439280513153 0.0 0.0 0.3740952954093453 0.0 44-45 0.0036767160755324267 0.0 0.0 0.48625626626524254 0.0 46-47 0.004310632640279397 0.0 0.0 0.6151948955347762 0.0 48-49 0.0044796770575452555 0.0 0.0 0.7555440229697554 0.0 50-51 0.0044796770575452555 0.0 0.0 0.9070923430485978 0.0 52-53 0.00452193816186172 0.0 0.0 1.0548371637389582 0.0 54-55 0.004564199266178184 0.0 0.0 1.2213036536415127 0.0 56-57 0.004648721474811114 0.0 0.0 1.4119012341087682 0.0 58-59 0.004733243683444043 0.0 0.0 1.6270947772882063 0.0 60-61 0.004733243683444043 0.0 0.0 1.8599957231762432 0.0 62-63 0.004817765892076973 0.0 0.0 2.0895580418232793 0.0 64-65 0.004817765892076973 0.0 0.0 2.3302772920098622 0.0 66-67 0.00515585472660869 0.0 0.0 2.6124546855308965 0.0 68-69 0.005240376935241619 0.0 0.0 2.933047422875598 0.0 70-71 0.005324899143874549 0.0 0.0 3.276714723177088 0.0 72-73 0.005493943561140408 0.0 0.0 3.6121411081368686 0.0 74-75 0.005662987978406266 0.0 0.0 3.9473984486793827 0.0 76-77 0.005747510187039196 0.0 0.0 4.314478400772195 0.0 78-79 0.005747510187039196 0.0 0.0 4.723988501598738 0.0 80-81 0.006339165647469701 0.0 0.0 5.162447458882058 0.0 82-83 0.0065504711690520245 0.0 0.0 5.62241731826246 0.0 84-85 0.006719515586317883 0.0 0.0 6.069962412973821 0.0 86-87 0.006888560003583741 0.0 0.0 6.528579917016096 0.0 88-89 0.006930821107900206 0.0 0.0 7.0256972870906695 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3260 0.0 54.123478 1 GTATCAA 6000 0.0 42.9613 1 TATCAAC 7810 0.0 32.700367 2 ATCAACG 7820 0.0 32.5867 3 TCAACGC 8010 0.0 31.813732 4 CAACGCA 8160 0.0 31.228924 5 AACGCAG 8370 0.0 30.584751 6 ACGCAGA 9595 0.0 26.580976 7 GTACATG 7450 0.0 26.55623 1 CGCAGAG 9680 0.0 26.298506 8 TACATGG 7430 0.0 26.24366 2 ACATGGG 7400 0.0 25.12045 3 GCAGAGT 10310 0.0 24.369053 9 TGGTATC 1525 0.0 24.325071 2 GTGTAGC 670 0.0 24.134232 1 GTGGTAT 1620 0.0 22.605028 1 GAGTACT 6090 0.0 22.109425 12-13 AGTACTT 6165 0.0 21.878971 12-13 GTACTTT 6365 0.0 21.042257 14-15 GTAGGAC 1440 0.0 20.79986 3 >>END_MODULE