FastQCFastQC Report
Thu 26 May 2016
SRR938640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181176
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55160.4669922179251864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46310.39206688926967703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.23832180809633788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.10718131760211858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.047.9961741
GTATCAA60800.041.6644331
TATCAAC76850.032.9678422
ATCAACG77550.032.363783
TCAACGC79000.031.7095954
CAACGCA80750.030.9622145
AACGCAG82400.030.3345036
GTCCTAC11900.029.265131
GTACATG70700.027.4630991
ACGCAGA94250.026.465667
TACATGG71650.026.1388952
CGCAGAG95750.026.0510528
GCAGAGT98150.024.9278559
ACATGGG72000.024.8894923
TAGGACC6150.023.9603824
TGACGCG1600.023.76060794-95
CTGGTCG1401.7771781E-523.7540669
CATGGGG34300.023.6978634
GAGTACT55900.023.07123412-13
CGACGAG1354.0017767E-1122.88058794-95
Produced by FastQC (version 0.11.5)