##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938640_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1181176 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10428505150799 31.0 31.0 34.0 28.0 34.0 2 31.26109826139373 31.0 31.0 34.0 28.0 34.0 3 31.302476514930884 31.0 31.0 34.0 27.0 34.0 4 34.97888121668574 37.0 35.0 37.0 32.0 37.0 5 34.98860119067776 37.0 35.0 37.0 32.0 37.0 6 34.975714034149014 37.0 35.0 37.0 32.0 37.0 7 34.90250817829011 37.0 35.0 37.0 32.0 37.0 8 34.86817121241881 37.0 35.0 37.0 32.0 37.0 9 36.333961238629975 39.0 35.0 39.0 32.0 39.0 10-11 36.161973744810254 39.0 35.0 39.0 31.5 39.0 12-13 35.98293522726503 38.0 35.0 39.0 30.0 39.0 14-15 37.105785251308866 39.0 36.0 41.0 31.0 41.0 16-17 37.066618353234404 39.0 36.0 41.0 31.0 41.0 18-19 37.11529611167175 39.0 36.0 41.0 31.0 41.0 20-21 37.07639081728718 39.0 36.0 41.0 31.0 41.0 22-23 36.91857436994995 39.0 36.0 41.0 30.5 41.0 24-25 36.78970788434577 39.0 36.0 41.0 30.0 41.0 26-27 36.58977959254167 39.0 36.0 41.0 30.0 41.0 28-29 36.3553373925647 39.0 35.5 40.5 29.5 41.0 30-31 36.07557849126633 38.5 35.0 40.0 29.0 41.0 32-33 35.764885165292895 38.0 35.0 40.0 28.0 41.0 34-35 35.47351199143904 38.0 34.5 40.0 27.0 41.0 36-37 35.23173218893713 38.0 34.0 40.0 25.5 41.0 38-39 34.98322984889636 38.0 34.0 40.0 25.0 41.0 40-41 34.680018049807984 38.0 33.5 40.0 24.5 41.0 42-43 34.26824749232968 38.0 33.0 40.0 24.0 41.0 44-45 33.75580734793121 38.0 33.0 40.0 21.5 41.0 46-47 33.181941979857356 37.5 32.0 40.0 17.0 41.0 48-49 32.66347775437361 37.0 31.0 40.0 13.5 41.0 50-51 31.955091789877205 36.5 30.5 39.5 9.0 40.5 52-53 31.979863288790156 37.0 31.0 39.0 9.0 40.0 54-55 32.00624631722961 37.0 31.0 39.0 7.5 40.5 56-57 31.620011751000696 36.5 30.0 39.0 4.0 41.0 58-59 31.202738626589095 36.0 29.5 39.0 2.0 40.0 60-61 30.702419876462102 35.5 28.5 39.0 2.0 40.0 62-63 30.021295725615825 35.0 28.0 38.5 2.0 40.0 64-65 29.391062381897363 34.5 26.5 38.0 2.0 40.0 66-67 28.721653250658665 34.0 26.0 38.0 2.0 40.0 68-69 27.9178924224671 34.0 24.5 37.5 2.0 39.5 70-71 27.111438092206413 33.0 22.5 36.5 2.0 39.0 72-73 26.243390485414537 32.0 20.0 36.0 2.0 39.0 74-75 25.331987358361495 31.0 15.5 35.5 2.0 38.0 76-77 24.4082215520803 31.0 7.5 35.0 2.0 37.5 78-79 23.438994273503695 30.0 4.0 34.5 2.0 36.5 80-81 22.46104517870326 29.0 2.0 34.0 2.0 36.0 82-83 21.4617525246026 28.5 2.0 34.0 2.0 35.5 84-85 20.474078799433784 26.5 2.0 33.5 2.0 35.0 86-87 19.470463334845952 26.0 2.0 33.0 2.0 35.0 88-89 18.369445366312895 24.5 2.0 32.0 2.0 34.5 90-91 17.204232476785847 20.0 2.0 31.5 2.0 34.0 92-93 16.025762883770074 12.0 2.0 31.0 2.0 34.0 94-95 14.744431397183824 2.0 2.0 31.0 2.0 34.0 96-97 13.28718920804351 2.0 2.0 30.0 2.0 34.0 98-99 11.568405131834714 2.0 2.0 29.0 2.0 34.0 100-101 8.205126924353356 2.0 2.0 11.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4639444403197075 1101 2 0.5290213243899444 1101 3 0.6327846831597554 1101 4 0.2421311114340341 1101 5 0.19271568044256782 1101 6 0.3387461671754508 1101 7 0.346920283036674 1101 8 0.33885857662688323 1101 9 0.2849733249468329 1101 10-11 0.48585581681521717 1101 12-13 0.4689979502268713 1101 14-15 0.6114155935257131 1101 16-17 0.54570766906172 1101 18-19 0.47091780737996913 1101 20-21 0.5667317748737162 1101 22-23 0.5876533941454412 1101 24-25 0.6174154175571758 1101 26-27 0.6561153426781985 1101 28-29 0.5990743870582165 1101 30-31 0.8824241806293927 1101 32-33 0.715937980980307 1101 34-35 0.6642044307212629 1101 36-37 0.8712453133993279 1101 38-39 0.9458955251804753 1101 40-41 0.8405452167031839 1101 42-43 1.0241877219449549 1101 44-45 1.1501990195991496 1101 46-47 1.4172314381516102 1101 48-49 1.2554096617131592 1101 50-51 1.4139973341896876 1101 52-53 1.4998557554185972 1101 54-55 1.5786321286684029 1101 56-57 1.8919184570771392 1101 58-59 1.7286376790820732 1101 60-61 1.4457842895071735 1101 62-63 1.6326804147895082 1101 64-65 1.7858730127642595 1101 66-67 2.043863796820549 1101 68-69 1.876735816231033 1101 70-71 2.215686187261081 1101 72-73 2.2067620604947145 1101 74-75 2.133728423038363 1101 76-77 2.302068904349831 1101 78-79 2.6665731991974546 1101 80-81 3.057143186743165 1101 82-83 3.2534409853509665 1101 84-85 3.2256776786809276 1101 86-87 3.4027562431196934 1101 88-89 3.3757367830559097 1101 90-91 3.851104503376142 1101 92-93 4.040907437272848 1101 94-95 3.927262805494644 1101 96-97 4.225283885448841 1101 98-99 4.224963125496515 1101 100-101 3.488292716447985 1102 1 0.6588017208627868 1102 2 0.43416107561739636 1102 3 0.4646536899552558 1102 4 0.391818923534629 1102 5 0.34202722580759115 1102 6 0.49863333953529576 1102 7 0.4872852355799253 1102 8 0.594861384222753 1102 9 0.7869498165694466 1102 10-11 0.6437241566320893 1102 12-13 0.5854219495843367 1102 14-15 0.7455612323236096 1102 16-17 0.6760583391831645 1102 18-19 0.7532701990745139 1102 20-21 0.5495627499196374 1102 22-23 0.5723189969055511 1102 24-25 0.8143233954595885 1102 26-27 0.6357698756884034 1102 28-29 0.5434750840683336 1102 30-31 0.5676547945989299 1102 32-33 0.4116368418785967 1102 34-35 0.661480207175174 1102 36-37 0.8714648750791341 1102 38-39 0.8917048216751766 1102 40-41 0.7792237784039457 1102 42-43 1.0242034361009615 1102 44-45 0.7279661951716676 1102 46-47 0.5586029696881738 1102 48-49 0.6222754034562499 1102 50-51 0.5653979892953913 1102 52-53 0.5608768787363054 1102 54-55 0.6552831626598632 1102 56-57 0.6890351713518754 1102 58-59 0.6646522548347846 1102 60-61 0.8190221215123685 1102 62-63 1.2073252748636776 1102 64-65 1.0910227162905173 1102 66-67 1.2749256198023282 1102 68-69 1.3926219549486198 1102 70-71 0.8922015557056575 1102 72-73 1.2776896496638166 1102 74-75 1.2333243073263027 1102 76-77 1.75710983099615 1102 78-79 1.8775226485035859 1102 80-81 2.0819466724921725 1102 82-83 2.3649294302082495 1102 84-85 2.5903499861475936 1102 86-87 2.9279883761243113 1102 88-89 3.1649261421278645 1102 90-91 3.141982261210522 1102 92-93 2.805412912783206 1102 94-95 2.5750810695343684 1102 96-97 2.6218537470944288 1102 98-99 2.46581705019428 1102 100-101 1.6059105551578874 1103 1 0.44165684421090745 1103 2 0.4436636205290583 1103 3 0.3890817041314527 1103 4 0.4092295213328967 1103 5 0.3409678093041464 1103 6 0.3763594997739048 1103 7 0.40849536502195605 1103 8 0.32238573511339297 1103 9 0.5423864481038905 1103 10-11 0.540970251736816 1103 12-13 0.6106964235093173 1103 14-15 0.6081017345679527 1103 16-17 0.7260305119531054 1103 18-19 0.7672308809324804 1103 20-21 0.6664420705718967 1103 22-23 0.7272316177516913 1103 24-25 0.7137997976381953 1103 26-27 0.9004142651573233 1103 28-29 0.7078047863747727 1103 30-31 0.8318309929864398 1103 32-33 1.1917488774446028 1103 34-35 1.0231363484879523 1103 36-37 1.2471357838157289 1103 38-39 1.3982355505339115 1103 40-41 1.290876947074942 1103 42-43 1.122132578229568 1103 44-45 1.1074788305507255 1103 46-47 1.3151184224710661 1103 48-49 1.09835100434276 1103 50-51 0.9863269563684298 1103 52-53 1.1291530933082008 1103 54-55 1.123041972414093 1103 56-57 1.308432980184591 1103 58-59 1.2884318202345533 1103 60-61 1.5827565523010065 1103 62-63 1.7398847855007809 1103 64-65 1.9330193737199757 1103 66-67 2.10153596591692 1103 68-69 2.3961263693216743 1103 70-71 2.241776798223686 1103 72-73 2.4100097800637066 1103 74-75 2.249983900206498 1103 76-77 2.2495535407209815 1103 78-79 2.6795909466001078 1103 80-81 2.7143155211880092 1103 82-83 2.74288968598756 1103 84-85 3.323405515096173 1103 86-87 3.4109737176973702 1103 88-89 3.537089333556086 1103 90-91 3.372830603632437 1103 92-93 3.277946528407414 1103 94-95 3.0348926040450888 1103 96-97 3.3160791427035097 1103 98-99 2.9703040878246227 1103 100-101 2.180899572897953 1104 1 0.45964382748237753 1104 2 0.42094154802181194 1104 3 0.47553680658734265 1104 4 0.2805812780827779 1104 5 0.4495697821520608 1104 6 0.46743528662497624 1104 7 0.48987534163252633 1104 8 0.4445192582740134 1104 9 0.677209374833943 1104 10-11 0.5579784380387451 1104 12-13 0.5636013530867814 1104 14-15 0.5535353924720567 1104 16-17 0.5164265433763049 1104 18-19 0.6680673074337236 1104 20-21 0.4891225124288141 1104 22-23 0.5762601299803052 1104 24-25 0.5212866410815309 1104 26-27 0.6205244115955182 1104 28-29 0.6593098456110553 1104 30-31 0.5733713906068161 1104 32-33 0.6708890615092784 1104 34-35 0.6323866154325799 1104 36-37 0.8415038877461711 1104 38-39 0.7239906224818426 1104 40-41 0.8291183540002507 1104 42-43 0.8117172886582011 1104 44-45 0.9110647867209636 1104 46-47 0.785857795532543 1104 48-49 0.9476067372964394 1104 50-51 1.0779413993439704 1104 52-53 0.9895712430005794 1104 54-55 1.3820672431722159 1104 56-57 1.6098150542172291 1104 58-59 1.7083643213785997 1104 60-61 1.60401996309065 1104 62-63 1.6124171366473696 1104 64-65 1.5626241685539597 1104 66-67 1.8150678340208515 1104 68-69 1.8210529595489575 1104 70-71 2.133789751393973 1104 72-73 2.1960441269391815 1104 74-75 2.177322525823012 1104 76-77 2.32715021281129 1104 78-79 2.4858701833927057 1104 80-81 2.8486474604771708 1104 82-83 2.8507591500644374 1104 84-85 2.8400974991303407 1104 86-87 2.8427391750624977 1104 88-89 2.7674990935601578 1104 90-91 2.470241558531505 1104 92-93 2.482167113651732 1104 94-95 2.736187921073613 1104 96-97 2.557045927164788 1104 98-99 2.871614023249446 1104 100-101 2.196889238327353 1105 1 0.5286171814367826 1105 2 0.31948557833950986 1105 3 0.24149772296574668 1105 4 0.3339309233137655 1105 5 0.2427043228412913 1105 6 0.3788824903839867 1105 7 0.43532001301370116 1105 8 0.23189396755231684 1105 9 0.4129558950280341 1105 10-11 0.3655664219354122 1105 12-13 0.5322177981949849 1105 14-15 0.5006317910647908 1105 16-17 0.6810550836877454 1105 18-19 0.6751390758480653 1105 20-21 0.8457476467215841 1105 22-23 0.511230334695604 1105 24-25 0.5175637062386116 1105 26-27 0.3460296327801231 1105 28-29 0.6802580410704877 1105 30-31 0.6614364532374211 1105 32-33 0.7453285736871251 1105 34-35 0.7874372039595556 1105 36-37 0.6558648017633786 1105 38-39 0.8787741012252184 1105 40-41 1.0015580108010695 1105 42-43 0.9693305077885839 1105 44-45 1.0138002270241415 1105 46-47 1.350970232518243 1105 48-49 1.4151883164612826 1105 50-51 1.265673460191877 1105 52-53 1.250455228809816 1105 54-55 1.5992717695762408 1105 56-57 1.702546052528973 1105 58-59 1.8107699327501017 1105 60-61 2.2237035929502866 1105 62-63 2.075030990347951 1105 64-65 2.19863543734019 1105 66-67 2.1337368156389758 1105 68-69 2.466429672888509 1105 70-71 2.3760203246912575 1105 72-73 2.659979141047298 1105 74-75 2.7435720240698274 1105 76-77 2.7092061169705275 1105 78-79 2.8579175872626976 1105 80-81 3.2161444056857285 1105 82-83 3.532881167584481 1105 84-85 3.0812721771228553 1105 86-87 3.2949778059256296 1105 88-89 3.4393425495180345 1105 90-91 3.2981988770444275 1105 92-93 3.1980770430521073 1105 94-95 3.2727406405758774 1105 96-97 3.222729624908668 1105 98-99 3.2214562661452106 1105 100-101 2.5330568622603895 1106 1 0.16976292356174127 1106 2 0.10850188111865933 1106 3 -0.09959534470189269 1106 4 0.07479889592858058 1106 5 -0.006198061906509622 1106 6 -0.16762434864328668 1106 7 0.09459033680587936 1106 8 -0.08994765883557676 1106 9 -0.08303053282007511 1106 10-11 0.12211906213011048 1106 12-13 0.21897470592055157 1106 14-15 0.26111286598364813 1106 16-17 -0.0047805401654059665 1106 18-19 0.34249537875180636 1106 20-21 0.33267697504265215 1106 22-23 0.039447854593881004 1106 24-25 0.3925310875290009 1106 26-27 0.20167497102143983 1106 28-29 0.3692181393038467 1106 30-31 0.12011572947238136 1106 32-33 0.27318200995137687 1106 34-35 0.1643907791431758 1106 36-37 0.13709753634295652 1106 38-39 0.29769731349497874 1106 40-41 0.4420964392749056 1106 42-43 -0.017379401308176057 1106 44-45 0.45339972118322436 1106 46-47 0.4890382220221099 1106 48-49 0.6615282722867306 1106 50-51 0.36159600036047834 1106 52-53 0.2836242169134202 1106 54-55 0.29113000122340793 1106 56-57 0.6563925684354857 1106 58-59 0.6616759541811987 1106 60-61 1.2520083267989186 1106 62-63 1.1747604886670047 1106 64-65 1.3572495799820672 1106 66-67 1.4751922365051229 1106 68-69 1.5863561224570404 1106 70-71 1.533482343361964 1106 72-73 1.751929293429452 1106 74-75 2.0134036803804136 1106 76-77 1.974041313752192 1106 78-79 2.1612065222350054 1106 80-81 2.23743430001149 1106 82-83 2.212825696743355 1106 84-85 2.278905564284269 1106 86-87 1.9650616213006167 1106 88-89 2.174675897156302 1106 90-91 1.8358735731626439 1106 92-93 2.0899950785509507 1106 94-95 2.039468587732527 1106 96-97 2.0618925647940305 1106 98-99 1.921922376257669 1106 100-101 1.4409980385273986 1107 1 -0.06137280962979119 1107 2 0.06005993920290109 1107 3 0.1114326749813479 1107 4 -1.1863767518605073E-5 1107 5 -0.012518102009359211 1107 6 -0.11948105908359707 1107 7 -0.03691007011623526 1107 8 0.05847726453128388 1107 9 0.03611652664618248 1107 10-11 -0.05259256617418373 1107 12-13 0.07029947082286725 1107 14-15 -0.027172743674412914 1107 16-17 0.1364353391340174 1107 18-19 0.0957529078041901 1107 20-21 0.12683365296893356 1107 22-23 0.31837889106324013 1107 24-25 0.23068038324582574 1107 26-27 0.19772130385525344 1107 28-29 0.08121396479287313 1107 30-31 0.1761772796701493 1107 32-33 0.2190186570456092 1107 34-35 0.12021373855625228 1107 36-37 0.19822127708621906 1107 38-39 0.35307083753317414 1107 40-41 0.41533195678778867 1107 42-43 0.7522358006927305 1107 44-45 0.5880303491411993 1107 46-47 0.6690143430776274 1107 48-49 0.5831538814282666 1107 50-51 0.6365528414529251 1107 52-53 0.7877470762680225 1107 54-55 0.9320872912876865 1107 56-57 0.9530066718460191 1107 58-59 0.9305306190846458 1107 60-61 0.8230067991027212 1107 62-63 1.0456142039751555 1107 64-65 1.0833549082447114 1107 66-67 1.0551542526932707 1107 68-69 1.294571446170373 1107 70-71 1.3131169676990169 1107 72-73 1.427465030064429 1107 74-75 1.6058802898086917 1107 76-77 1.9041910968484714 1107 78-79 2.0594802983728826 1107 80-81 2.2256441539499576 1107 82-83 2.3309874020946673 1107 84-85 2.2605040099676152 1107 86-87 2.325783340213789 1107 88-89 2.3274468413943943 1107 90-91 2.1401172178603716 1107 92-93 2.1301846797540396 1107 94-95 2.1448518130058023 1107 96-97 1.8804513254728388 1107 98-99 1.8844822308914484 1107 100-101 1.5126481159717269 1108 1 0.38687663609877987 1108 2 0.19176866760204092 1108 3 -0.0454401334844583 1108 4 0.1759209632526222 1108 5 0.10690891498829558 1108 6 0.4000057091601832 1108 7 0.3387126793845283 1108 8 0.32681696608953814 1108 9 0.33990821493336654 1108 10-11 0.2755964993477704 1108 12-13 0.42020622743088865 1108 14-15 0.3954157432039054 1108 16-17 0.3002773442600457 1108 18-19 0.3997933499634101 1108 20-21 0.4575583726884176 1108 22-23 0.4281390998781234 1108 24-25 0.476431283445514 1108 26-27 0.50338446406721 1108 28-29 0.5258839963981288 1108 30-31 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0.18154947806932142 2316 90-91 0.5763327626187724 2316 92-93 0.8718869371174112 2316 94-95 0.8859048500987452 2316 96-97 0.8103406768089574 2316 98-99 0.46264417854016315 2316 100-101 0.7782064212135076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3498.0 3 1301.0 4 1441.0 5 1611.0 6 1988.0 7 2515.0 8 3271.0 9 4186.0 10 5500.0 11 7278.0 12 9123.0 13 11251.0 14 13336.0 15 15801.0 16 19196.0 17 22496.0 18 20790.0 19 18814.0 20 20075.0 21 22177.0 22 24602.0 23 27167.0 24 30460.0 25 33798.0 26 37396.0 27 41931.0 28 46536.0 29 51497.0 30 57150.0 31 64535.0 32 72435.0 33 81849.0 34 93206.0 35 104100.0 36 105895.0 37 81344.0 38 21307.0 39 320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87409423215193 19.283017713360064 14.780992430899879 22.061895623588125 2 14.759064202687208 20.626004569574224 42.09597460820728 22.518956619531288 3 19.25421179531348 25.887223056408796 29.72354972217776 25.13501542609996 4 12.21733713562651 17.111759130881833 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29.392373775841698 21.46825751324447 50-51 21.235998533492175 28.453977309770988 29.107767157167814 21.202256999569023 52-53 21.284961870938215 28.20995982827211 29.3215935420423 21.183484758747372 54-55 20.974249079659025 28.04665036525768 29.569785044924835 21.409315510158464 56-57 21.136734833840926 28.28886930208024 29.02494100673374 21.549454857345097 58-59 21.21740632307596 28.11116266232282 28.97757578733658 21.693855227264645 60-61 20.961416508833707 27.832674767018233 29.68391356878032 21.521995155367748 62-63 21.17518507134868 27.88749829598038 29.6078595735421 21.329457059128845 64-65 20.959376164188708 27.983074812883125 29.297465032004606 21.76008399092356 66-67 20.889058783338434 28.31049706402918 29.03319757299288 21.767246579639504 68-69 21.32255762149769 27.7021758290697 29.46572427871 21.509542270722616 70-71 21.35134849084601 27.792225935984522 29.087945607376536 21.768479965792935 72-73 21.25403501493352 27.926049950574374 28.9187959197929 21.901119114699203 74-75 20.98742905719199 28.261040745704907 29.090467267879532 21.66106292922357 76-77 21.164951137583106 27.67871241128672 29.26059744014698 21.895739010983196 78-79 21.15260379403335 27.86802589188571 28.942776223154524 22.03659409092641 80-81 21.249589346374904 28.150687019281246 28.72505173388968 21.874671900454175 82-83 21.196586334839942 28.0624004037195 28.85349757582018 21.88751568562037 84-85 21.224526427997496 27.741417367368935 28.923431749157757 22.110624455475808 86-87 21.143931636377797 27.957011550986767 28.573907985788484 22.325148826846952 88-89 21.410560256322327 27.619001653665116 28.60823716589064 22.362200924121918 90-91 21.370741017989424 27.725384362647297 28.678415738291292 22.225458881071983 92-93 21.368066647729126 27.65610665871911 28.688724810564608 22.28710188298716 94-95 21.282213253985326 27.51562714338698 28.558763309110063 22.643396293517636 96-97 21.709396570995036 27.17145194177059 28.27252528400046 22.846626203233914 98-99 21.702120633802462 27.02457618651688 28.167530578411633 23.105772601269027 100-101 22.356900958507072 26.415458167398214 28.49724551588464 22.730395358210075 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 392.0 1 295.5 2 234.0 3 282.5 4 239.0 5 223.5 6 409.0 7 790.5 8 1226.0 9 1441.0 10 1309.5 11 1142.5 12 1181.0 13 1325.5 14 1518.5 15 1693.0 16 1907.0 17 2147.0 18 2299.5 19 2506.5 20 2910.5 21 3337.0 22 3851.0 23 4600.5 24 5438.0 25 6502.5 26 7960.5 27 9818.0 28 11793.0 29 13953.5 30 16670.0 31 19930.0 32 23596.5 33 27075.5 34 30867.5 35 34680.0 36 37699.0 37 40009.5 38 40809.0 39 40453.5 40 40385.0 41 41004.5 42 41773.0 43 42490.5 44 43008.0 45 42928.0 46 42347.0 47 42212.0 48 42840.5 49 42258.5 50 40223.0 51 38035.0 52 35426.5 53 33148.5 54 31290.0 55 28526.0 56 25215.5 57 22371.5 58 19883.5 59 16953.0 60 13933.0 61 11502.5 62 9513.0 63 7402.0 64 5507.0 65 4178.5 66 3029.0 67 2273.5 68 1728.0 69 1279.5 70 1001.5 71 787.0 72 609.5 73 503.0 74 398.5 75 290.5 76 213.5 77 148.5 78 97.5 79 57.5 80 31.0 81 15.5 82 8.5 83 5.5 84 4.0 85 4.0 86 3.0 87 1.5 88 1.5 89 1.5 90 1.0 91 1.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3852093168164609 2 0.027260967036241847 3 0.032594634499854384 4 0.041060773330985394 5 0.06273408873868076 6 0.052151415199767015 7 0.03547312170243892 8 0.03852093168164609 9 0.03471116920763714 10-11 0.03576943656152851 12-13 0.0285308878609115 14-15 0.02628736107066178 16-17 0.019514450005756974 18-19 0.024340149139501648 20-21 0.030478099792071632 22-23 0.029673816603114186 24-25 0.03911356139982526 26-27 0.0331449335238779 28-29 0.028149911613510603 30-31 0.030943737427783835 32-33 0.02569473135248261 34-35 0.027218636342086194 36-37 0.029250509661557636 38-39 0.012487554775918237 40-41 0.01100598048047031 42-43 0.011471618116182516 44-45 0.012021917140206033 46-47 0.02040339458302573 48-49 0.017905883627842085 50-51 0.011767932975272101 52-53 0.010286358679824175 54-55 0.007788847724640527 56-57 0.009228091325932799 58-59 0.0035557783090750237 60-61 0.006349604123348256 62-63 0.012275901305139962 64-65 0.008127493277885768 66-67 0.00427540010972116 68-69 0.005333667463612535 70-71 0.011302295339559896 72-73 0.009185760631777144 74-75 0.0100747052090459 76-77 0.0045293842746550895 78-79 0.009397414102555419 80-81 0.011175303257092931 82-83 0.013376499353186992 84-85 0.008381477442819698 86-87 0.0018202198486931666 88-89 0.013799806294743545 90-91 0.02201196096094062 92-93 0.008508469525286663 94-95 0.018456182651865598 96-97 0.016254986555771535 98-99 0.00965139826748935 100-101 0.11619775545727308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1181176.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.62993502480033 #Duplication Level Percentage of deduplicated Percentage of total 1 85.23163491562364 60.19904836148001 2 9.572691125124788 13.52237104360093 3 2.4279579385293886 5.144595343238366 4 0.9487427950392153 2.6803856787546896 5 0.4879758692210032 1.723285196837496 6 0.28112178845146024 1.1913368191429379 7 0.19643328919524802 0.9711849316797727 8 0.14363816220425116 0.8116123250854392 9 0.10289571708896278 0.6540766031091311 >10 0.5486498696804911 7.095176177442557 >50 0.036935944748632994 1.7390500756962135 >100 0.019876991421089934 2.657896123471786 >500 9.637291145479196E-4 0.3993897219238488 >1k 3.6139841795546985E-4 0.7412618784406841 >5k 1.2046613931848995E-4 0.46932972009628254 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5516 0.4669922179251864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4631 0.39206688926967703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2815 0.23832180809633788 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1266 0.10718131760211858 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.466138831131009E-5 0.0 0.0 0.0 0.0 3 8.466138831131009E-5 0.0 0.0 0.0 0.0 4 8.466138831131009E-5 0.0 0.0 0.0 0.0 5 8.466138831131009E-5 0.0 0.0 0.0 0.0 6 8.466138831131009E-5 0.0 0.0 0.0 0.0 7 3.3864555324524036E-4 0.0 0.0 0.0 0.0 8 3.3864555324524036E-4 0.0 0.0 0.0 0.0 9 3.3864555324524036E-4 0.0 0.0 0.0 0.0 10-11 3.3864555324524036E-4 0.0 0.0 0.0 0.0 12-13 3.809762474008954E-4 0.0 0.0 8.466138831131009E-5 0.0 14-15 5.079683298678605E-4 0.0 0.0 8.466138831131009E-5 0.0 16-17 5.502990240235156E-4 0.0 0.0 8.466138831131009E-5 0.0 18-19 5.926297181791705E-4 0.0 0.0 8.466138831131009E-5 0.0 20-21 5.926297181791705E-4 0.0 0.0 8.466138831131009E-5 0.0 22-23 6.772911064904807E-4 0.0 0.0 1.2699208246696515E-4 0.0 24-25 0.001185259436358341 0.0 0.0 5.502990240235156E-4 0.0 26-27 0.001227590130513996 0.0 0.0 0.0017778891545375119 0.0 28-29 0.0015239049896035813 0.0 0.0 0.00495269121621164 0.0 30-31 0.0016085663779148915 0.0 0.0 0.016085663779148916 0.0 32-33 0.0016085663779148915 0.0 0.0 0.03598109003230679 0.0 34-35 0.001947211931160132 0.0 0.0 0.06578189871788793 0.0 36-37 0.002370518872716682 0.0 0.0 0.11602843268065047 0.0 38-39 0.0026245030376506125 0.0 0.0 0.19484818519848016 0.0 40-41 0.002709164425961923 0.0 0.0 0.2837426429253558 0.0 42-43 0.0030054792850515077 0.0 0.0 0.37348371453534446 0.0 44-45 0.003217132755829783 0.0 0.0 0.483247204480958 0.0 46-47 0.0033441248382967484 0.0 0.0 0.6110012394427249 0.0 48-49 0.0033864555324524034 0.0 0.0 0.7554335679018198 0.0 50-51 0.0033864555324524034 0.0 0.0 0.9092209797693147 0.0 52-53 0.0033864555324524034 0.0 0.0 1.061696140117984 0.0 54-55 0.0033864555324524034 0.0 0.0 1.2228067620744072 0.0 56-57 0.0036404396973863337 0.0 0.0 1.4175279551904203 0.0 58-59 0.0036404396973863337 0.0 0.0 1.6271495526492243 0.0 60-61 0.0037251010856976436 0.0 0.0 1.8599683705053267 0.0 62-63 0.0037251010856976436 0.0 0.0 2.09346447946792 0.0 64-65 0.0037251010856976436 0.0 0.0 2.331447642011013 0.0 66-67 0.003979085250631574 0.0 0.0 2.6012634865591577 0.0 68-69 0.003979085250631574 0.0 0.0 2.9093039479298595 0.0 70-71 0.004021415944787229 0.0 0.0 3.2435894396770677 0.0 72-73 0.00419073872140985 0.0 0.0 3.5792295136372565 0.0 74-75 0.0044023921921881246 0.0 0.0 3.9022550407390604 0.0 76-77 0.0044870535804994345 0.0 0.0 4.2576212181757835 0.0 78-79 0.0044870535804994345 8.466138831131009E-5 0.0 4.651508327294154 0.0 80-81 0.005502990240235156 8.466138831131009E-5 0.0 5.072741064837078 0.0 82-83 0.005587651628546466 8.466138831131009E-5 0.0 5.504641137307226 0.0 84-85 0.005672313016857776 8.466138831131009E-5 0.0 5.928159732334555 0.0 86-87 0.0057569744051690856 8.466138831131009E-5 0.0 6.352270957080062 0.0 88-89 0.0057569744051690856 8.466138831131009E-5 0.0 6.798013166539111 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3290 0.0 47.996174 1 GTATCAA 6080 0.0 41.664433 1 TATCAAC 7685 0.0 32.967842 2 ATCAACG 7755 0.0 32.36378 3 TCAACGC 7900 0.0 31.709595 4 CAACGCA 8075 0.0 30.962214 5 AACGCAG 8240 0.0 30.334503 6 GTCCTAC 1190 0.0 29.26513 1 GTACATG 7070 0.0 27.463099 1 ACGCAGA 9425 0.0 26.46566 7 TACATGG 7165 0.0 26.138895 2 CGCAGAG 9575 0.0 26.051052 8 GCAGAGT 9815 0.0 24.927855 9 ACATGGG 7200 0.0 24.889492 3 TAGGACC 615 0.0 23.960382 4 TGACGCG 160 0.0 23.760607 94-95 CTGGTCG 140 1.7771781E-5 23.754066 9 CATGGGG 3430 0.0 23.697863 4 GAGTACT 5590 0.0 23.071234 12-13 CGACGAG 135 4.0017767E-11 22.880587 94-95 >>END_MODULE