FastQCFastQC Report
Thu 26 May 2016
SRR938640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181176
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56990.4824852519861562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.4133168977358158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32930.2787899517091441No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.1151394881033817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.048.33771
GTATCAA58550.042.44261
TATCAAC75650.032.783252
ATCAACG76950.032.158133
TCAACGC77800.031.7457374
CAACGCA79300.031.2051455
AACGCAG81800.030.3095076
TAGGACC6900.028.2225634
GTGTAGC5800.027.0341761
ACGCAGA94050.026.2606967
CGCAGAG95200.025.893588
TACATGG74250.024.7630922
GTACATG74900.024.613711
GCACCGT1551.411594E-624.5143266
GCAGAGT101950.023.8530759
GTAGGAC13550.023.8358863
ACATGGG73850.023.2819713
GTCCTAC10850.022.7719781
GTACTAG2303.614332E-922.7243791
TGGTATC16000.022.5675262
Produced by FastQC (version 0.11.5)