##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938640_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1181176 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800883187602864 31.0 31.0 34.0 30.0 34.0 2 32.04823497937649 31.0 31.0 34.0 30.0 34.0 3 32.09511622315387 33.0 31.0 34.0 30.0 34.0 4 35.74827121445068 37.0 35.0 37.0 33.0 37.0 5 35.6557024524711 37.0 35.0 37.0 33.0 37.0 6 35.697119650246876 37.0 35.0 37.0 33.0 37.0 7 35.62370044768942 37.0 35.0 37.0 33.0 37.0 8 35.59887264895325 37.0 35.0 37.0 33.0 37.0 9 37.128069821940166 39.0 37.0 39.0 33.0 39.0 10-11 37.056681222781364 39.0 37.0 39.0 33.0 39.0 12-13 36.9373471861941 39.0 37.0 39.0 33.0 39.0 14-15 37.91021490446809 40.0 37.0 41.0 33.0 41.0 16-17 38.02255125400448 40.0 37.0 41.0 33.0 41.0 18-19 38.20352767072816 40.0 37.5 41.0 33.0 41.0 20-21 38.19008894525456 40.0 37.5 41.0 33.0 41.0 22-23 38.061295268444326 40.0 37.0 41.0 33.0 41.0 24-25 37.940717132755836 40.0 37.0 41.0 33.0 41.0 26-27 37.7278153298069 40.0 37.0 41.0 32.0 41.0 28-29 37.591680664016195 39.5 37.0 41.0 32.0 41.0 30-31 37.31503349204522 39.0 36.0 41.0 31.5 41.0 32-33 37.16420372577838 39.0 36.0 41.0 31.0 41.0 34-35 37.01237156867393 39.0 36.0 41.0 31.0 41.0 36-37 36.87632452742013 39.0 35.0 41.0 31.0 41.0 38-39 36.657618762995526 39.0 35.0 40.5 30.0 41.0 40-41 36.406629494673105 39.0 35.0 40.0 30.0 41.0 42-43 36.12416185225572 38.5 35.0 40.0 30.0 41.0 44-45 35.77233452085041 38.0 35.0 40.0 28.5 41.0 46-47 35.251414691798686 38.0 34.5 40.0 26.5 41.0 48-49 34.785509102792474 38.0 34.0 40.0 24.5 41.0 50-51 34.38536509377096 38.0 33.0 40.0 24.0 41.0 52-53 33.816673806443745 38.0 33.0 40.0 23.0 41.0 54-55 33.42138047166553 37.0 32.5 40.0 21.5 41.0 56-57 33.05151179841108 37.0 32.0 40.0 19.0 41.0 58-59 32.54932838120652 36.5 31.0 39.0 17.0 40.0 60-61 32.09156764106281 36.0 31.0 39.0 13.5 40.0 62-63 31.614215832356905 35.5 30.0 39.0 9.5 40.0 64-65 30.996153833129014 35.0 29.0 38.0 7.5 40.0 66-67 30.35490223302878 34.5 28.0 38.0 4.5 40.0 68-69 29.63615244468225 34.0 27.5 37.5 2.0 39.5 70-71 28.875536329894953 34.0 26.0 37.0 2.0 39.0 72-73 28.31614213292515 33.0 26.0 36.0 2.0 39.0 74-75 27.614112545463165 32.5 25.5 36.0 2.0 38.5 76-77 26.446503315339967 31.0 24.0 34.5 2.0 36.5 78-79 26.956421396980637 32.0 25.0 35.0 2.0 37.0 80-81 26.561236005472512 32.0 24.0 35.0 2.0 37.0 82-83 25.877076320548333 31.5 21.5 35.0 2.0 36.0 84-85 25.081543732686747 31.0 19.0 34.0 2.0 36.0 86-87 24.172444665316597 30.5 14.0 34.0 2.0 35.0 88-89 23.186772335367465 30.0 4.5 34.0 2.0 35.0 90-91 22.32176830548538 29.0 2.0 34.0 2.0 35.0 92-93 21.253245494320915 29.0 2.0 34.0 2.0 35.0 94-95 20.07921681442901 26.5 2.0 33.0 2.0 34.5 96-97 18.666194961631458 25.5 2.0 32.5 2.0 34.0 98-99 16.887678889513502 12.5 2.0 32.0 2.0 34.0 100-101 12.705841043163762 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1906687089966148 1101 2 0.26497450498753494 1101 3 0.2276440099426651 1101 4 0.01236300701504689 1101 5 2.447519294292988E-4 1101 6 0.20041178076626665 1101 7 0.14936393541659498 1101 8 0.07304864497802299 1101 9 0.2311736564575213 1101 10-11 0.23427530526900853 1101 12-13 0.19086746420705225 1101 14-15 0.3252290789000867 1101 16-17 0.19504846112885588 1101 18-19 0.2317124068418579 1101 20-21 0.32891703528877514 1101 22-23 0.3229026817277898 1101 24-25 0.3410774280803466 1101 26-27 0.5360406150459269 1101 28-29 0.5880703578853357 1101 30-31 0.4404815741331163 1101 32-33 0.29393935656333525 1101 34-35 0.09186238292983262 1101 36-37 0.12921469493867477 1101 38-39 0.23205100178581262 1101 40-41 0.2820842243658177 1101 42-43 0.5306814409998211 1101 44-45 0.5901675210819306 1101 46-47 0.5769709166026757 1101 48-49 0.6924320446927155 1101 50-51 0.7082694946480856 1101 52-53 0.7451854354942142 1101 54-55 0.7989750939493163 1101 56-57 0.8781343009127056 1101 58-59 0.9484969494581676 1101 60-61 0.6984745841865987 1101 62-63 0.9522807764566643 1101 64-65 1.1258821726705577 1101 66-67 1.2906131426372696 1101 68-69 1.1384888372190858 1101 70-71 1.2764190841003007 1101 72-73 1.3684763952365593 1101 74-75 1.4476017336459392 1101 76-77 1.6925980806235081 1101 78-79 1.8974571557960438 1101 80-81 1.713200343718288 1101 82-83 2.2471675514353677 1101 84-85 2.3190392639451396 1101 86-87 2.472479828497427 1101 88-89 2.3741722980491637 1101 90-91 2.66695270018651 1101 92-93 3.0705718561121422 1101 94-95 2.7427464827261865 1101 96-97 3.0043240058167555 1101 98-99 2.9834950909836557 1101 100-101 2.4378353369625856 1102 1 0.29576585740110417 1102 2 0.2517746139390198 1102 3 0.2892312793810774 1102 4 0.21014573960201943 1102 5 0.13182199922834315 1102 6 0.2540319731075371 1102 7 0.2908655452445714 1102 8 0.23466088246431838 1102 9 0.3405275951234188 1102 10-11 0.3232593327024347 1102 12-13 0.4169500822552905 1102 14-15 0.594245826698355 1102 16-17 0.4795934140911484 1102 18-19 0.30358895643224315 1102 20-21 0.25564279882215146 1102 22-23 0.38596664532681757 1102 24-25 0.4294804682457283 1102 26-27 0.5422219529142467 1102 28-29 0.42018206109032263 1102 30-31 0.3428455943031139 1102 32-33 0.38799294532736184 1102 34-35 0.5380291764737635 1102 36-37 0.609555412761317 1102 38-39 0.7797971553096232 1102 40-41 0.8158220808152521 1102 42-43 0.6830856195685087 1102 44-45 0.6281189538637548 1102 46-47 0.4601475157799513 1102 48-49 0.5711920930224679 1102 50-51 0.6390735181704272 1102 52-53 0.7122882863913205 1102 54-55 0.8910879844460879 1102 56-57 1.0342670994991252 1102 58-59 1.0473695460653047 1102 60-61 1.0802364728186262 1102 62-63 1.1750821914291087 1102 64-65 1.3960656965525757 1102 66-67 1.351885255946808 1102 68-69 1.6070971915628256 1102 70-71 1.7469323433559367 1102 72-73 1.5917837187316657 1102 74-75 1.4395560857687464 1102 76-77 1.4072639278260404 1102 78-79 1.74795223901123 1102 80-81 1.988885568221196 1102 82-83 2.042202576543094 1102 84-85 1.976510384818429 1102 86-87 2.3028935298446562 1102 88-89 2.0731130766332093 1102 90-91 2.41535727684715 1102 92-93 2.457800961522704 1102 94-95 2.5579807458719515 1102 96-97 2.171523935277655 1102 98-99 2.310438146374306 1102 100-101 2.007666863749984 1103 1 0.2988169439016737 1103 2 0.2855190526216731 1103 3 0.28668719345191107 1103 4 0.20421201630212238 1103 5 0.20489448874977967 1103 6 0.05583521064473729 1103 7 0.23228963883654785 1103 8 0.17911624494495726 1103 9 0.26610007638752364 1103 10-11 0.31521338226628615 1103 12-13 0.33826012549071294 1103 14-15 0.4323714439343931 1103 16-17 0.4042935075642262 1103 18-19 0.3966475075666338 1103 20-21 0.15371999502642097 1103 22-23 0.3213129250656266 1103 24-25 0.2676713184543118 1103 26-27 0.504760473397404 1103 28-29 0.40365169555446556 1103 30-31 0.3738857546936529 1103 32-33 0.5276043054430986 1103 34-35 0.493679838216849 1103 36-37 0.5134053141687005 1103 38-39 0.3814932951691148 1103 40-41 0.5021509838113474 1103 42-43 0.38215889849148965 1103 44-45 0.6832552660043589 1103 46-47 0.8882669730416239 1103 48-49 0.9711441178869649 1103 50-51 0.8537193113868611 1103 52-53 0.9269464656151953 1103 54-55 1.07972646496372 1103 56-57 1.1775063211954517 1103 58-59 1.0364244441532335 1103 60-61 1.1118509310876945 1103 62-63 1.2098282452471416 1103 64-65 1.3320321023974486 1103 66-67 1.641961421635255 1103 68-69 1.4103768411874462 1103 70-71 1.5180207596314794 1103 72-73 1.9415208050803052 1103 74-75 1.8079580596028926 1103 76-77 1.5494028591943447 1103 78-79 1.4198291485207122 1103 80-81 1.9138007653568891 1103 82-83 2.407183535120982 1103 84-85 2.574593939173184 1103 86-87 2.4715979699806034 1103 88-89 2.4536756325766795 1103 90-91 2.557603966480226 1103 92-93 2.684279933384996 1103 94-95 2.352615375167005 1103 96-97 2.3677410008838606 1103 98-99 2.4010327077610114 1103 100-101 2.3314284281522664 1104 1 0.054883578365497954 1104 2 0.22957508557026785 1104 3 0.18387855461527636 1104 4 0.1997756508167896 1104 5 0.1340206900006109 1104 6 0.10802924125438551 1104 7 0.23289662007415757 1104 8 0.12026131028283515 1104 9 0.14827262431043664 1104 10-11 0.09882616001581113 1104 12-13 0.24810593318888863 1104 14-15 0.205581325970293 1104 16-17 0.24082646886996173 1104 18-19 0.36026041243265183 1104 20-21 0.49079479036887363 1104 22-23 0.3142611378077831 1104 24-25 0.23618615294864043 1104 26-27 0.3934652199271227 1104 28-29 0.2569735672176634 1104 30-31 0.3604733760898995 1104 32-33 0.41549550643969013 1104 34-35 0.3643801229577903 1104 36-37 0.49371179202501025 1104 38-39 0.6102966205364808 1104 40-41 0.48468676710315606 1104 42-43 0.45014238605949686 1104 44-45 0.5877383467995969 1104 46-47 0.6292099231611061 1104 48-49 0.6149605362527595 1104 50-51 0.6153695376542103 1104 52-53 0.5574569654372326 1104 54-55 0.7689044026258003 1104 56-57 0.8532512238242234 1104 58-59 0.8131284406956709 1104 60-61 0.7131437057864289 1104 62-63 0.7746385874447306 1104 64-65 0.9519156636930219 1104 66-67 0.7230935210911973 1104 68-69 1.1547471696564564 1104 70-71 1.2538711053883311 1104 72-73 1.3936249007735988 1104 74-75 1.3231325905430467 1104 76-77 1.4585002685602895 1104 78-79 1.570335072149124 1104 80-81 1.7464360173017717 1104 82-83 1.5739603694119317 1104 84-85 2.2750486984145546 1104 86-87 1.9902030571825158 1104 88-89 1.7892822324648137 1104 90-91 2.0397822346451697 1104 92-93 2.0201169319104473 1104 94-95 2.089792499283547 1104 96-97 2.3571792095582893 1104 98-99 2.5155312967679784 1104 100-101 2.0147558293827554 1105 1 0.11739538432278351 1105 2 0.12877287077268562 1105 3 0.11840788061317653 1105 4 0.06918700578651027 1105 5 0.03941807852285706 1105 6 0.12046386576591317 1105 7 0.15366462711050843 1105 8 0.16875723132459086 1105 9 0.058238180430052466 1105 10-11 0.2705076529971677 1105 12-13 0.15506982466853003 1105 14-15 0.2766609364917585 1105 16-17 0.20768899144885467 1105 18-19 0.23224989332471324 1105 20-21 0.24206097928779968 1105 22-23 0.21783967103564805 1105 24-25 0.11341710993954024 1105 26-27 0.40834478440535804 1105 28-29 0.3136403175098579 1105 30-31 0.2909735502277755 1105 32-33 0.2989422999416931 1105 34-35 0.3644384148718913 1105 36-37 0.4039990604806718 1105 38-39 0.42148099417141793 1105 40-41 0.4410102591872658 1105 42-43 0.13795118562975972 1105 44-45 0.24821414163566402 1105 46-47 0.46074186417854435 1105 48-49 0.5925491399621947 1105 50-51 0.41109105873375285 1105 52-53 0.5983808406246496 1105 54-55 0.7965724013979738 1105 56-57 0.7857846051684518 1105 58-59 0.6821715973725233 1105 60-61 0.8033648313663946 1105 62-63 0.8464911192322724 1105 64-65 1.0166344457904728 1105 66-67 1.005723711285171 1105 68-69 1.2877837061427684 1105 70-71 1.2303968040058528 1105 72-73 1.2503961337877776 1105 74-75 1.3702036801148445 1105 76-77 1.3747964003383828 1105 78-79 1.5326106113862252 1105 80-81 1.53460246359057 1105 82-83 1.820067354996855 1105 84-85 1.8424035294122731 1105 86-87 2.0451372832429833 1105 88-89 1.8305219170096372 1105 90-91 2.2800364169055385 1105 92-93 2.2421748135675266 1105 94-95 2.5264365673803084 1105 96-97 2.402501910643256 1105 98-99 2.5426469981008673 1105 100-101 2.2373556782665016 1106 1 -0.039278940097883464 1106 2 0.06657686503317706 1106 3 0.02599847064323768 1106 4 0.018029102160696198 1106 5 0.011524150067366179 1106 6 -0.09268079933597306 1106 7 -0.06533860049783158 1106 8 -0.014451591404750275 1106 9 -0.08864672415026575 1106 10-11 0.06064112494991747 1106 12-13 -0.0064597557423908825 1106 14-15 -0.044323086050972904 1106 16-17 -0.0946433587663762 1106 18-19 -0.1266974619814647 1106 20-21 -0.021830091872551804 1106 22-23 0.1022019328671604 1106 24-25 0.08389414948275231 1106 26-27 -0.08661722561317475 1106 28-29 0.003185302949972879 1106 30-31 0.16818634465272453 1106 32-33 -0.10813435073805522 1106 34-35 -0.2583875524588777 1106 36-37 -0.2362496238691989 1106 38-39 -0.11504676928219482 1106 40-41 -0.02183902057086584 1106 42-43 0.08832298700621521 1106 44-45 0.26113530368541404 1106 46-47 -0.15972309692472209 1106 48-49 -0.1055583865325147 1106 50-51 0.3683963691634986 1106 52-53 0.12378121149158972 1106 54-55 -0.18962495269688873 1106 56-57 0.18778588845943034 1106 58-59 0.23999236993584816 1106 60-61 0.3532560561894229 1106 62-63 0.2606224094519156 1106 64-65 0.21580099882520187 1106 66-67 0.14438281756367743 1106 68-69 0.4781125788747751 1106 70-71 0.3346385859945116 1106 72-73 0.16852611441381882 1106 74-75 0.1734040167883819 1106 76-77 0.11796100697924317 1106 78-79 0.5088316586042723 1106 80-81 0.5698991952131713 1106 82-83 0.7164976741653071 1106 84-85 0.8130276559749419 1106 86-87 0.8209828951699762 1106 88-89 0.7963869997971322 1106 90-91 1.092808484630634 1106 92-93 1.3764503805793389 1106 94-95 1.063277193576468 1106 96-97 1.0001341211085126 1106 98-99 1.2298268242682404 1106 100-101 1.2592706059031489 1107 1 0.04506353137949404 1107 2 -0.059645805130791985 1107 3 -0.0425571445839239 1107 4 -0.02291938063898158 1107 5 0.031127266911958884 1107 6 -0.04020539511233068 1107 7 0.026448327270017558 1107 8 -0.021778176033620866 1107 9 -0.058548394020881744 1107 10-11 -0.12720292951738088 1107 12-13 -0.10742974680930928 1107 14-15 0.1510515107517918 1107 16-17 0.06544887249782505 1107 18-19 -0.098187750482289 1107 20-21 -0.07366978376398237 1107 22-23 -0.017614942249139176 1107 24-25 -0.047574802922916604 1107 26-27 -0.14874982858366792 1107 28-29 -0.15940143701785559 1107 30-31 -0.07637682495598597 1107 32-33 -0.19296585717099646 1107 34-35 -0.11248759901155836 1107 36-37 0.11328256469631981 1107 38-39 0.02837324386910467 1107 40-41 0.14507486755833554 1107 42-43 0.08598707528547322 1107 44-45 0.14512078295623354 1107 46-47 0.23207369762828023 1107 48-49 0.14168735667811205 1107 50-51 0.11268704332980661 1107 52-53 0.06572610715199545 1107 54-55 0.025638169299618596 1107 56-57 0.2584956414123454 1107 58-59 0.24757886820054864 1107 60-61 0.5020455208467993 1107 62-63 0.6652874122298478 1107 64-65 0.7139657105660859 1107 66-67 0.7533167610925524 1107 68-69 1.0323424829153218 1107 70-71 0.8432359330874881 1107 72-73 1.0463594213695053 1107 74-75 1.159719977737101 1107 76-77 1.1614179046898165 1107 78-79 1.2267654345964623 1107 80-81 1.3455351227605057 1107 82-83 1.6747035615237031 1107 84-85 1.5807976367115337 1107 86-87 1.7040363274537 1107 88-89 1.7334792913215615 1107 90-91 1.8676875171824143 1107 92-93 1.6666520669387417 1107 94-95 1.2821238127457164 1107 96-97 1.299613234316297 1107 98-99 1.5534924740159077 1107 100-101 1.474017211629727 1108 1 -0.10082133740362664 1108 2 -0.006224417329484311 1108 3 0.0369944065898693 1108 4 0.0032139275359313046 1108 5 -0.08545873557243766 1108 6 0.030368374090620875 1108 7 -0.09241199633014929 1108 8 -0.07764810700520997 1108 9 -0.06698904373705972 1108 10-11 0.054814146759802895 1108 12-13 -0.005026488793994588 1108 14-15 0.17124240025916038 1108 16-17 0.16175404412541639 1108 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-0.47889767333660416 2316 50-51 -0.5245355034521495 2316 52-53 -0.4417331222988352 2316 54-55 -0.32006512342947957 2316 56-57 -0.2280400684239865 2316 58-59 -0.21020367113064964 2316 60-61 -0.20532335601120622 2316 62-63 -0.4311933119455773 2316 64-65 -0.33803027134404573 2316 66-67 -0.335408698865038 2316 68-69 0.3728532230203818 2316 70-71 0.24606607384158607 2316 72-73 0.3686829223570953 2316 74-75 0.32352426942478374 2316 76-77 0.37627624948353855 2316 78-79 0.28864758675409874 2316 80-81 -0.023969788574209616 2316 82-83 0.4675139988810493 2316 84-85 0.6369248004263213 2316 86-87 0.6851164098475238 2316 88-89 0.23875167354568916 2316 90-91 0.5255118460997217 2316 92-93 0.835388557895616 2316 94-95 1.092096102198166 2316 96-97 1.0251530215099365 2316 98-99 1.1488873341991983 2316 100-101 0.8971379805097275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 20.0 5 47.0 6 98.0 7 230.0 8 521.0 9 1048.0 10 1810.0 11 2843.0 12 4040.0 13 5936.0 14 7775.0 15 10865.0 16 14157.0 17 18250.0 18 17528.0 19 13871.0 20 13885.0 21 14726.0 22 16424.0 23 18128.0 24 21047.0 25 23826.0 26 27572.0 27 32376.0 28 38346.0 29 44241.0 30 51963.0 31 60526.0 32 70657.0 33 84629.0 34 102660.0 35 124502.0 36 146306.0 37 138999.0 38 50225.0 39 1084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.319303507781 20.143637547644193 15.52305085405995 22.014008090514857 2 14.980876683609662 21.445802311393805 41.0724975635447 22.500823441451832 3 19.14583606723927 26.04095144159742 30.30042627766889 24.51278621349442 4 12.52192729957263 17.821137578142462 37.555876516285466 32.10105860599944 5 12.699335913821189 38.764887755551264 34.23227099863526 14.303505331992291 6 30.43102806017054 37.25761444526472 18.010271119629927 14.30108637493481 7 27.704254065439866 33.61785203898487 20.84016268532378 17.83773121025148 8 25.991638841290378 36.3248152688507 19.782657283927204 17.900888605931716 9 26.710227883637707 16.804143000220968 20.632710026389024 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20.84098887272641 27.75252272954341 31.043332187471957 20.363156210258225 36-37 20.720011006106272 27.386790555914192 30.914120622691627 20.979077815287905 38-39 20.73855277490719 27.712663293649364 30.79617574179356 20.75260818964988 40-41 21.38373220326067 27.226185285065345 30.83277944532173 20.557303066352254 42-43 20.710115030794963 27.6970515659521 30.774896845659434 20.8179365575935 44-45 20.722810576319862 27.97326711633495 30.421058156890602 20.88286415045459 46-47 21.191703681378154 27.78111955515464 29.78154370924872 21.24563305421849 48-49 21.092157307632394 27.949814422236823 29.619336999735857 21.33869127039493 50-51 21.135334615671162 28.492197606453228 29.218549987470116 21.153917790405494 52-53 21.297630497063942 28.257812552913368 29.246234261447913 21.198322688574773 54-55 20.94831760889148 28.159393689001472 29.44929460131259 21.44299410079446 56-57 21.082759893529836 28.37417963114726 28.97239700095498 21.57066347436792 58-59 21.186089117963792 28.121719371202936 28.94903045778106 21.743161053052212 60-61 20.93566073133894 27.924542997825895 29.590171151462606 21.549625119372557 62-63 20.99682858439386 28.034433479853977 29.50521344829221 21.463524487459956 64-65 20.878777116525022 28.095655556689074 29.128397939171613 21.897169387614287 66-67 20.76827962747154 28.44222875340287 28.9361451745153 21.853346444610285 68-69 21.20367806983456 27.828292962105994 29.282452972197742 21.685575995861704 70-71 21.101131414793393 27.95569838872446 29.00621922558535 21.936950970896802 72-73 21.1025217283821 28.064959100014054 28.80117577935173 22.031343392252115 74-75 20.83749585159197 28.336674636125352 28.891503044423523 21.93432646785915 76-77 20.79533448021294 27.765421918494788 29.473211443510532 21.966032157781736 78-79 20.714609846458107 27.911589805414266 29.19852756913449 22.17527277899314 80-81 20.775846413887194 28.201675535663867 29.04388809612805 21.97858995432089 82-83 20.670414946611988 28.21479489723696 29.079916082779363 22.03487407337169 84-85 20.58004512685048 27.959766830494914 29.059321065264605 22.400866977390006 86-87 20.42198518962537 28.195759743940453 28.820753658673908 22.56150140776027 88-89 20.63993850196753 27.8980016525903 28.74093276531186 22.72112708013031 90-91 20.474779834704588 27.97381891330372 29.074645390371145 22.476755861620546 92-93 20.399586512029163 27.947646869081616 29.00587637183778 22.646890247051445 94-95 20.259521129586584 27.8199556289476 28.88715521105881 23.033368030406997 96-97 20.50401464303372 27.546741552486676 28.7625637500254 23.186680054454207 98-99 20.39882286805692 27.474906364504527 28.713798790358087 23.412471977080468 100-101 20.086760990741432 27.464027376106525 29.12389855533807 23.325313077813973 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 461.0 1 316.5 2 220.0 3 283.5 4 247.5 5 231.0 6 421.0 7 896.0 8 1355.0 9 1556.0 10 1450.5 11 1249.5 12 1268.5 13 1429.0 14 1621.0 15 1808.0 16 1988.5 17 2198.0 18 2433.5 19 2699.0 20 2967.5 21 3441.0 22 4016.5 23 4625.0 24 5470.0 25 6538.0 26 7927.5 27 9783.5 28 11856.5 29 14158.5 30 16954.5 31 20011.5 32 23985.0 33 27682.5 34 31379.5 35 35367.0 36 38373.0 37 40482.0 38 41053.0 39 40938.5 40 40803.0 41 41046.0 42 42147.5 43 43069.0 44 43347.5 45 43466.5 46 42975.5 47 42849.5 48 43304.0 49 42403.0 50 40650.5 51 37984.0 52 34703.5 53 32605.0 54 30978.0 55 28236.0 56 24719.5 57 21707.5 58 19129.0 59 16298.0 60 13278.5 61 10701.5 62 8709.0 63 6555.5 64 4707.0 65 3520.5 66 2545.0 67 1926.0 68 1455.0 69 1048.5 70 799.0 71 628.5 72 500.0 73 412.0 74 331.5 75 278.5 76 197.5 77 107.5 78 61.0 79 36.5 80 23.0 81 12.0 82 3.5 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024297818445345995 2 0.013291837964875684 3 0.0017778891545375119 4 0.0 5 3.3864555324524036E-4 6 0.0 7 0.0 8 0.0 9 2.5398416493393024E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001227590130513996 26-27 0.007577194253862252 28-29 2.116534707782752E-4 30-31 0.0 32-33 2.963148590895853E-4 34-35 0.00965139826748935 36-37 0.0011429287422026861 38-39 0.011556279504493826 40-41 0.011090641868781621 42-43 0.00486802982790033 44-45 7.619524948017908E-4 46-47 1.2699208246696512E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.2330694155655045E-5 66-67 3.809762474008954E-4 68-69 0.0011005980480470311 70-71 0.0 72-73 1.6932277662262018E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2699208246696512E-4 82-83 0.001015936659735721 84-85 0.005164344686989915 86-87 0.0037674317798532986 88-89 0.0 90-91 0.0015239049896035816 92-93 4.233069415565504E-4 94-95 4.2330694155655045E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1181176.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.47366972979272 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95214930447938 57.485117445811674 2 10.233855766936939 14.014993196951488 3 2.7134091426085067 5.573912444183247 4 1.0617517250654298 2.9080814782867166 5 0.5600640540791596 1.9174820533272574 6 0.3145367391880748 1.292249087823011 7 0.21493229116918577 1.030204190385053 8 0.15409860178178558 0.8441357411383067 9 0.10971972888893025 0.6761621230904705 >10 0.6191134424867812 7.683808706238121 >50 0.042083089828042985 1.9377704125412336 >100 0.022427488843309102 2.8179489002146187 >500 0.0013629914060144062 0.5256120907626844 >1k 3.7172492891301984E-4 0.808992943570976 >5k 1.239083096376733E-4 0.48352918567498604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5699 0.4824852519861562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4882 0.4133168977358158 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3293 0.2787899517091441 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1360 0.1151394881033817 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 8.466138831131009E-5 0.0 0.0 0.0 0.0 12-13 8.466138831131009E-5 0.0 0.0 0.0 0.0 14-15 2.5398416493393024E-4 0.0 0.0 0.0 0.0 16-17 3.809762474008954E-4 0.0 0.0 0.0 0.0 18-19 5.079683298678605E-4 0.0 0.0 0.0 0.0 20-21 6.772911064904807E-4 0.0 0.0 0.0 0.0 22-23 6.772911064904807E-4 0.0 0.0 1.2699208246696515E-4 0.0 24-25 0.0011005980480470311 0.0 0.0 4.6563763571220545E-4 0.0 26-27 0.0011005980480470311 0.0 0.0 0.0016932277662262017 0.0 28-29 0.0012699208246696513 0.0 0.0 0.00495269121621164 0.0 30-31 0.0014392436012922714 0.0 0.0 0.016297317249927192 0.0 32-33 0.0014392436012922714 0.0 0.0 0.036235074197240716 0.0 34-35 0.0015662356837592365 0.0 0.0 0.06654385121268973 0.0 36-37 0.0017778891545375119 0.0 0.0 0.11649407031636269 0.0 38-39 0.001989542625315787 0.0 0.0 0.19599111394068286 0.0 40-41 0.002031873319471442 0.0 0.0 0.2846739181967801 0.0 42-43 0.002412849566872337 0.0 0.0 0.3752192729957263 0.0 44-45 0.0028784872025845428 0.0 0.0 0.48633734515432075 0.0 46-47 0.003174802061674128 0.0 0.0 0.6147686712225782 0.0 48-49 0.0033864555324524034 0.0 0.0 0.7600476135647862 0.0 50-51 0.0033864555324524034 0.0 0.0 0.9145123165387715 0.0 52-53 0.0034287862266080583 0.0 0.0 1.0668181541108184 0.0 54-55 0.0034711169207637133 0.0 0.0 1.2269128394075057 0.0 56-57 0.0036404396973863337 0.0 0.0 1.4218033553001415 0.0 58-59 0.0037251010856976436 0.0 0.0 1.6318482597005017 0.0 60-61 0.0037251010856976436 0.0 0.0 1.8643284320033593 0.0 62-63 0.0038097624740089536 0.0 0.0 2.0965122894471273 0.0 64-65 0.003894423862320264 0.0 0.0 2.3339874836603522 0.0 66-67 0.003979085250631574 0.0 0.0 2.6045229500091436 0.0 68-69 0.004063746638942884 0.0 0.0 2.9159075362181417 0.0 70-71 0.00419073872140985 0.0 0.0 3.253367830027024 0.0 72-73 0.0044447228863437795 0.0 0.0 3.594637886309915 0.0 74-75 0.0046140456629663994 0.0 0.0 3.924097678923378 0.0 76-77 0.004656376357122054 0.0 0.0 4.291612765582775 0.0 78-79 0.004698707051277709 0.0 0.0 4.697987429477063 0.0 80-81 0.0059686278759473604 0.0 0.0 5.136194775376405 0.0 82-83 0.006137950652569981 0.0 0.0 5.58633937702764 0.0 84-85 0.006307273429192601 0.0 0.0 6.032674216204867 0.0 86-87 0.006518926899970877 0.0 0.0 6.481294912866499 0.0 88-89 0.006603588288282187 0.0 0.0 6.961663630144873 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 48.3377 1 GTATCAA 5855 0.0 42.4426 1 TATCAAC 7565 0.0 32.78325 2 ATCAACG 7695 0.0 32.15813 3 TCAACGC 7780 0.0 31.745737 4 CAACGCA 7930 0.0 31.205145 5 AACGCAG 8180 0.0 30.309507 6 TAGGACC 690 0.0 28.222563 4 GTGTAGC 580 0.0 27.034176 1 ACGCAGA 9405 0.0 26.260696 7 CGCAGAG 9520 0.0 25.89358 8 TACATGG 7425 0.0 24.763092 2 GTACATG 7490 0.0 24.61371 1 GCACCGT 155 1.411594E-6 24.514326 6 GCAGAGT 10195 0.0 23.853075 9 GTAGGAC 1355 0.0 23.835886 3 ACATGGG 7385 0.0 23.281971 3 GTCCTAC 1085 0.0 22.771978 1 GTACTAG 230 3.614332E-9 22.724379 1 TGGTATC 1600 0.0 22.567526 2 >>END_MODULE