FastQCFastQC Report
Thu 26 May 2016
SRR938639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479060
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77800.3138286285930958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59080.23831613595475704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.17115358240623463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61550.037.244941
GTATCAA97450.034.4076961
ATCAACG120000.027.9407863
TCAACGC121250.027.574294
TATCAAC120950.027.5247212
CAACGCA122550.027.2429735
AACGCAG126850.026.3346256
GTACATG97450.026.257221
TACATGG93900.026.134542
ACATGGG99800.023.9223523
ACGCAGA141850.023.3526347
CGCAGAG144050.022.9959818
GAGTACT74800.022.39406612-13
GCAGAGT151350.021.132349
GTACTTT79950.020.91739514-15
GGACCGA8450.020.8068966
AGAGTAC121550.020.37010610-11
CATGGGG50250.019.6860224
GAGTACA74350.019.6383821
AGTACTT78050.019.4828512-13
Produced by FastQC (version 0.11.5)