##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938639_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2479060 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01182706348374 31.0 31.0 34.0 28.0 34.0 2 31.036948682161785 31.0 31.0 34.0 27.0 34.0 3 31.084331964534943 31.0 31.0 34.0 27.0 34.0 4 34.74112486184279 37.0 35.0 37.0 32.0 37.0 5 34.71409969907949 37.0 35.0 37.0 32.0 37.0 6 34.73207667422329 37.0 35.0 37.0 31.0 37.0 7 34.636527554798995 37.0 35.0 37.0 30.0 37.0 8 34.606182585334764 37.0 35.0 37.0 30.0 37.0 9 36.075742821876034 39.0 35.0 39.0 30.0 39.0 10-11 35.90317418699023 38.0 35.0 39.0 30.0 39.0 12-13 35.75133397336087 38.0 35.0 39.0 30.0 39.0 14-15 36.67592918283543 39.0 36.0 41.0 30.0 41.0 16-17 36.5020142715384 39.0 36.0 40.5 30.0 41.0 18-19 36.66409425346704 39.0 36.0 41.0 30.0 41.0 20-21 36.64723383056481 39.0 36.0 41.0 30.0 41.0 22-23 36.5568479585004 39.0 36.0 41.0 30.0 41.0 24-25 36.412696546271576 39.0 36.0 41.0 29.0 41.0 26-27 36.1883219849459 39.0 35.0 40.5 28.0 41.0 28-29 35.97676296660831 39.0 35.0 40.0 27.0 41.0 30-31 35.723845126781924 38.0 34.5 40.0 27.0 41.0 32-33 35.44256169677216 38.0 34.0 40.0 26.5 41.0 34-35 35.18096818955572 38.0 34.0 40.0 25.5 41.0 36-37 34.90305034972933 38.0 34.0 40.0 25.0 41.0 38-39 34.75204593676635 38.0 33.5 40.0 24.5 41.0 40-41 34.64160750445734 38.0 33.0 40.0 24.0 41.0 42-43 34.34122046259469 38.0 33.0 40.0 24.0 41.0 44-45 33.98143489871161 38.0 33.0 40.0 23.0 41.0 46-47 33.584135519108045 38.0 32.5 40.0 20.0 41.0 48-49 33.216726702863184 37.5 32.0 40.0 18.5 41.0 50-51 32.5249015755972 36.5 30.5 39.5 15.5 40.5 52-53 32.553156438327434 37.0 31.0 39.0 15.0 40.0 54-55 32.65848950812001 37.0 31.0 40.0 14.5 41.0 56-57 32.32563330455899 37.0 31.0 40.0 11.0 41.0 58-59 31.94457072438747 36.0 30.5 39.0 8.0 41.0 60-61 31.450783764814084 36.0 30.0 39.0 7.0 40.0 62-63 30.872437133429607 35.0 29.0 39.0 2.0 40.0 64-65 30.344238542028027 35.0 28.0 38.0 2.0 40.0 66-67 29.785461626584272 34.0 28.0 38.0 2.0 40.0 68-69 29.170712891176493 34.0 26.5 37.5 2.0 39.5 70-71 28.514812469242376 34.0 26.0 37.0 2.0 39.0 72-73 27.856553492049407 33.5 25.5 36.0 2.0 39.0 74-75 27.161922059167587 33.0 24.5 35.5 2.0 38.5 76-77 26.383506046646716 32.0 21.5 35.0 2.0 37.5 78-79 25.56530842335401 31.5 19.5 35.0 2.0 37.0 80-81 24.832124676288593 31.0 17.0 34.5 2.0 36.0 82-83 24.06241720652183 30.5 9.5 34.0 2.0 36.0 84-85 23.326432397763668 30.0 4.5 34.0 2.0 35.0 86-87 22.51694251046768 29.5 2.0 34.0 2.0 35.0 88-89 21.661536832509096 29.0 2.0 34.0 2.0 35.0 90-91 20.71152937000315 28.0 2.0 34.0 2.0 35.0 92-93 19.729705815914095 26.0 2.0 33.0 2.0 35.0 94-95 18.68218518309359 25.0 2.0 33.0 2.0 34.5 96-97 17.395208466112155 22.0 2.0 32.0 2.0 34.0 98-99 15.858775301928958 4.5 2.0 32.0 2.0 34.0 100-101 12.439331238453285 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4775721389511034 1101 2 0.3620640775053836 1101 3 0.3573829698673663 1101 4 0.20145070003975718 1101 5 0.24500565555563725 1101 6 0.42522716793411774 1101 7 0.43445108185448333 1101 8 0.6305528518376136 1101 9 0.7104413802835694 1101 10-11 0.6557591669420546 1101 12-13 0.6503884178820485 1101 14-15 0.7899719465107893 1101 16-17 0.7676907932937524 1101 18-19 0.9262521829717301 1101 20-21 0.8131797663485756 1101 22-23 0.7540540433524612 1101 24-25 0.787032412444951 1101 26-27 0.7618053848810149 1101 28-29 0.8542143009075289 1101 30-31 0.6916281169523444 1101 32-33 0.7949125293084407 1101 34-35 0.7570699686366495 1101 36-37 0.7128068291509706 1101 38-39 0.8518716566064697 1101 40-41 0.8019684420091551 1101 42-43 0.9685678742295707 1101 44-45 0.8932475500461337 1101 46-47 0.7750810733698827 1101 48-49 0.47495168547175126 1101 50-51 0.7432992881770701 1101 52-53 0.7847642035389768 1101 54-55 0.7654306056392599 1101 56-57 0.7644799578302184 1101 58-59 0.7433960565228084 1101 60-61 0.9351455347269173 1101 62-63 0.8118065507368826 1101 64-65 0.7408195186877826 1101 66-67 0.8236989798842949 1101 68-69 0.6662823121284234 1101 70-71 0.8889350190270058 1101 72-73 0.8617784611187425 1101 74-75 0.6858410619261406 1101 76-77 0.7095723336669053 1101 78-79 1.1002241410953602 1101 80-81 1.033957557067069 1101 82-83 1.2004913792421625 1101 84-85 1.118431576212469 1101 86-87 1.2821000033783143 1101 88-89 1.3246417288137842 1101 90-91 1.1484543850791447 1101 92-93 0.9160959532053283 1101 94-95 0.9189113075402879 1101 96-97 1.1026739640510819 1101 98-99 1.2468660601422812 1101 100-101 1.0892425770211958 1102 1 0.5057035929487981 1102 2 0.5240563780404663 1102 3 0.4404665698773158 1102 4 0.45846206898933417 1102 5 0.43525990949378013 1102 6 0.4130110537089209 1102 7 0.35305351974199084 1102 8 0.5966628412065944 1102 9 0.6891389745883174 1102 10-11 0.7270352744836543 1102 12-13 0.6343904344944136 1102 14-15 0.7635017463383988 1102 16-17 0.9341671182862967 1102 18-19 0.8416345840814827 1102 20-21 0.7702469940905914 1102 22-23 0.8091813902238485 1102 24-25 0.734769022827443 1102 26-27 0.7046851282369886 1102 28-29 0.7697173779176794 1102 30-31 0.8556823621665046 1102 32-33 0.8660227187524825 1102 34-35 0.7126210420303138 1102 36-37 0.9147356428068392 1102 38-39 0.707551931621353 1102 40-41 0.905195184004981 1102 42-43 0.9612581050512929 1102 44-45 1.085764364461923 1102 46-47 1.2596273482066707 1102 48-49 1.199631826742774 1102 50-51 1.2020668835164372 1102 52-53 0.9046425074360442 1102 54-55 1.00096977546837 1102 56-57 1.070080545293834 1102 58-59 0.9786993721089452 1102 60-61 1.0730561887776808 1102 62-63 1.155506655893447 1102 64-65 1.0915493780692636 1102 66-67 0.937397397921476 1102 68-69 1.1456163614060095 1102 70-71 1.312090155782812 1102 72-73 1.0818884194851073 1102 74-75 1.1527412576391995 1102 76-77 1.1347104665664887 1102 78-79 1.1091895764172222 1102 80-81 1.2189864273567892 1102 82-83 1.438524097101368 1102 84-85 1.3389401525829179 1102 86-87 1.4542607130355272 1102 88-89 1.572369975698475 1102 90-91 1.5949003311079082 1102 92-93 1.7624648973750112 1102 94-95 1.6283579864565958 1102 96-97 1.6570962716626916 1102 98-99 1.3797206733503344 1102 100-101 1.32708486308238 1103 1 0.487598845189968 1103 2 0.3933178360257372 1103 3 0.4580078166268038 1103 4 0.3917819923069388 1103 5 0.4415584700605848 1103 6 0.5052081241695063 1103 7 0.30432802901339073 1103 8 0.4379461937292177 1103 9 0.5900884282323844 1103 10-11 0.6337708637250614 1103 12-13 0.6682605948206088 1103 14-15 0.8790615931768642 1103 16-17 0.846440471644712 1103 18-19 0.8735889110631021 1103 20-21 0.6925959943055844 1103 22-23 0.8419198176460228 1103 24-25 0.8124877443599985 1103 26-27 0.8313218174442838 1103 28-29 0.8637365401059256 1103 30-31 0.9678077614663607 1103 32-33 0.8958727654568364 1103 34-35 1.049904430998275 1103 36-37 1.1470540026544427 1103 38-39 1.0834978677645566 1103 40-41 1.0870220311485 1103 42-43 1.1974972299286222 1103 44-45 1.053166724683095 1103 46-47 0.8914846569952388 1103 48-49 0.9474954377714795 1103 50-51 1.2743315951242948 1103 52-53 1.2401045055482527 1103 54-55 1.076219062462627 1103 56-57 1.1178128129280793 1103 58-59 1.0602182210950524 1103 60-61 1.1581535144782897 1103 62-63 1.2291988683480497 1103 64-65 1.3866830317127388 1103 66-67 1.2999830510385912 1103 68-69 1.3471058193289025 1103 70-71 1.3625483770715512 1103 72-73 1.501284208331331 1103 74-75 1.6429718918038674 1103 76-77 1.723646642893243 1103 78-79 1.6854970047657716 1103 80-81 1.8515094768434253 1103 82-83 1.5699193560884304 1103 84-85 1.8117132065814197 1103 86-87 1.9844395562709813 1103 88-89 2.1174553129851894 1103 90-91 2.0659406545462744 1103 92-93 2.138032275082068 1103 94-95 1.9499571763888532 1103 96-97 2.2117054944010093 1103 98-99 2.2089195925357714 1103 100-101 1.8453570316168406 1104 1 0.5299586462532524 1104 2 0.3217055176289918 1104 3 0.311018408618704 1104 4 0.3060334071585942 1104 5 0.4025632801478878 1104 6 0.45603743548586806 1104 7 0.4002929455922626 1104 8 0.5627226349179395 1104 9 0.7296871248538963 1104 10-11 0.723263803758222 1104 12-13 0.8199865913416673 1104 14-15 0.8538882585368839 1104 16-17 0.9061214869079564 1104 18-19 0.9577164396255498 1104 20-21 0.9596946705107641 1104 22-23 0.9879496581536529 1104 24-25 0.8895063068870499 1104 26-27 1.0375961639175628 1104 28-29 0.8726298933655912 1104 30-31 0.9300620509372521 1104 32-33 0.9043169594609637 1104 34-35 0.9240010084047441 1104 36-37 1.1073319914009616 1104 38-39 1.0718115605886354 1104 40-41 1.211002235475128 1104 42-43 1.1733202121827446 1104 44-45 1.0301493544696996 1104 46-47 1.0076249906797372 1104 48-49 0.8433844056035085 1104 50-51 0.842938629774892 1104 52-53 1.0954221428072302 1104 54-55 1.0858065709354037 1104 56-57 1.2302251247916018 1104 58-59 1.1386577601759988 1104 60-61 1.2177120317627477 1104 62-63 1.3978214194092828 1104 64-65 1.3929222620987716 1104 66-67 1.3212895328149763 1104 68-69 1.499436139211955 1104 70-71 1.4679578878146238 1104 72-73 1.49882608222374 1104 74-75 1.3706507184365577 1104 76-77 1.4002560621711595 1104 78-79 1.5976537041802317 1104 80-81 1.6510578676283103 1104 82-83 1.706373937921164 1104 84-85 1.6327681591747059 1104 86-87 1.597452108230538 1104 88-89 1.540265253287842 1104 90-91 1.612391888100916 1104 92-93 1.3925994080577055 1104 94-95 1.381133728642407 1104 96-97 1.6340744419800437 1104 98-99 1.7792905980808484 1104 100-101 1.739371780373487 1105 1 0.4492494394936237 1105 2 0.2810969665162375 1105 3 0.32045295166669163 1105 4 0.3182228983396769 1105 5 0.2529111115881548 1105 6 0.4256098798482242 1105 7 0.4441335587606119 1105 8 0.401142618295907 1105 9 0.48227277449836947 1105 10-11 0.7145064546647362 1105 12-13 0.5884901466099279 1105 14-15 0.6074319837192661 1105 16-17 0.7015088726314787 1105 18-19 0.7087937936440838 1105 20-21 0.628612237537574 1105 22-23 0.6545740001981599 1105 24-25 0.8390592049693595 1105 26-27 0.6800146697814498 1105 28-29 0.635846718557211 1105 30-31 0.7821759734293323 1105 32-33 0.8853362902702102 1105 34-35 0.7804974531364834 1105 36-37 0.6698570014049707 1105 38-39 0.8371061399588413 1105 40-41 0.8772456559202269 1105 42-43 0.8005672648499669 1105 44-45 0.9475817120281107 1105 46-47 0.8636597122606773 1105 48-49 0.8776327203140113 1105 50-51 1.071299204774121 1105 52-53 0.9932081802991561 1105 54-55 0.9268622239658484 1105 56-57 1.0012027466095645 1105 58-59 1.0170591207202158 1105 60-61 1.131566034902466 1105 62-63 1.1442623034552142 1105 64-65 1.2329890580479201 1105 66-67 1.3835259658497279 1105 68-69 1.1077063703813472 1105 70-71 1.2583009481156644 1105 72-73 1.2396224007511556 1105 74-75 1.1477615627742956 1105 76-77 1.2990078705868306 1105 78-79 1.424888444230099 1105 80-81 1.4788081985299044 1105 82-83 1.490445258757191 1105 84-85 1.6556003689817054 1105 86-87 1.8489911853998713 1105 88-89 1.916377236542683 1105 90-91 2.042833295433084 1105 92-93 1.9738819210629082 1105 94-95 2.0128556481794533 1105 96-97 2.135763579173382 1105 98-99 1.9619752103873083 1105 100-101 1.6776624812692305 1106 1 0.2857844010498205 1106 2 0.14729140001999852 1106 3 0.1359186421960814 1106 4 0.14335382878741143 1106 5 0.24041804155304192 1106 6 0.10856717508681868 1106 7 0.3911278051008722 1106 8 0.19480186375572828 1106 9 0.2398759804199031 1106 10-11 0.1361304962140366 1106 12-13 0.14157515542365928 1106 14-15 0.48143083323620317 1106 16-17 0.425559226331913 1106 18-19 0.20006141566590685 1106 20-21 0.43770228259245414 1106 22-23 0.37332996961236375 1106 24-25 0.2418205976449741 1106 26-27 0.35589375643852605 1106 28-29 0.46588003527728716 1106 30-31 0.0606319959188113 1106 32-33 0.4581223103766021 1106 34-35 -0.06777691560671428 1106 36-37 0.19479694170926365 1106 38-39 0.2768864561830142 1106 40-41 0.3535812647087013 1106 42-43 -0.09912503226461666 1106 44-45 0.20476997560757582 1106 46-47 0.30811546035155146 1106 48-49 0.6155486815398064 1106 50-51 0.3772734100603685 1106 52-53 0.24915402795129893 1106 54-55 0.3940259347808208 1106 56-57 -0.3806501408913654 1106 58-59 0.053793612376569655 1106 60-61 0.6330298830411927 1106 62-63 0.5504648786955002 1106 64-65 0.7210566073945586 1106 66-67 0.04279359222708834 1106 68-69 0.2736273852704265 1106 70-71 0.25171110372643213 1106 72-73 0.5495445860343047 1106 74-75 0.9332008276597961 1106 76-77 0.8525599325897169 1106 78-79 1.0740670995026917 1106 80-81 1.0729041328882793 1106 82-83 0.7931958248209874 1106 84-85 0.8052121665041518 1106 86-87 0.7795856037214826 1106 88-89 0.7719419905701521 1106 90-91 1.0329420344376956 1106 92-93 1.160381431687611 1106 94-95 1.0555661651128325 1106 96-97 1.0178117656847938 1106 98-99 1.1331617541532637 1106 100-101 0.6636589744425532 1107 1 0.27983412836098864 1107 2 0.12028665221048485 1107 3 0.1901296275743718 1107 4 0.1563437440593134 1107 5 0.23533787934927375 1107 6 0.36034383889829513 1107 7 0.23455324545689393 1107 8 0.2057202594996852 1107 9 0.31719127545030545 1107 10-11 0.3315486070412348 1107 12-13 0.3590006012009255 1107 14-15 0.43321733059124057 1107 16-17 0.6170037121384695 1107 18-19 0.4860304247940874 1107 20-21 0.5233147994133844 1107 22-23 0.5601987705645755 1107 24-25 0.5769387266634709 1107 26-27 0.4850160893345432 1107 28-29 0.5157233009413886 1107 30-31 0.5825826336384239 1107 32-33 0.6911769162096206 1107 34-35 0.6358011539948976 1107 36-37 0.7501598672321492 1107 38-39 0.662858085581604 1107 40-41 0.5523329166837811 1107 42-43 0.7251430689850409 1107 44-45 0.6489202670900625 1107 46-47 0.5723695055069484 1107 48-49 0.6529821808838392 1107 50-51 0.7179599148846805 1107 52-53 0.6837915331165476 1107 54-55 0.7221063035944226 1107 56-57 0.9676365787348828 1107 58-59 0.7820938580193904 1107 60-61 0.6022919275508549 1107 62-63 0.6904123919685219 1107 64-65 0.663374591966889 1107 66-67 0.7574863519681685 1107 68-69 1.0786034444319164 1107 70-71 1.209495460290423 1107 72-73 1.2563940030090777 1107 74-75 1.3256002514412515 1107 76-77 1.602132557450119 1107 78-79 1.475318067798021 1107 80-81 1.6406936155444996 1107 82-83 1.286159043452738 1107 84-85 1.5730011672715598 1107 86-87 1.7025058318476454 1107 88-89 1.6116576400297724 1107 90-91 1.5397979569114213 1107 92-93 1.7491750170246974 1107 94-95 1.753741282302169 1107 96-97 1.5379104444661422 1107 98-99 1.805730429332499 1107 100-101 1.542157788393716 1108 1 0.43524057108269787 1108 2 0.24154408735602217 1108 3 0.242731422720194 1108 4 0.26441190342789156 1108 5 0.14880962520118146 1108 6 0.22710388869204934 1108 7 0.36169661434245626 1108 8 0.4645347301554068 1108 9 0.45507469570056003 1108 10-11 0.4790789724255333 1108 12-13 0.41380571648585374 1108 14-15 0.5035463990052875 1108 16-17 0.6236361356805418 1108 18-19 0.5712556689429604 1108 20-21 0.5384555405596387 1108 22-23 0.5915665160166057 1108 24-25 0.6434444522313285 1108 26-27 0.7474233210921071 1108 28-29 0.6034419519399989 1108 30-31 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2316 84-85 -2.6471886748174747 2316 86-87 -2.610884315583835 2316 88-89 -3.0375457338072245 2316 90-91 -3.0774456029595783 2316 92-93 -3.091493084774669 2316 94-95 -3.1353463187541255 2316 96-97 -3.1884875347557333 2316 98-99 -3.217229520263974 2316 100-101 -2.9523454099351305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9114.0 3 3262.0 4 3526.0 5 4104.0 6 5010.0 7 6178.0 8 7776.0 9 9524.0 10 12143.0 11 14886.0 12 18019.0 13 20930.0 14 24018.0 15 27086.0 16 30982.0 17 34899.0 18 34454.0 19 32909.0 20 35452.0 21 38870.0 22 42345.0 23 46726.0 24 52293.0 25 58479.0 26 64705.0 27 72468.0 28 81971.0 29 92698.0 30 106223.0 31 121930.0 32 141772.0 33 166430.0 34 198314.0 35 236138.0 36 268198.0 37 248189.0 38 102826.0 39 4213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0480282192413 18.34795286250704 14.308944860493222 23.295074057758434 2 15.48685288051054 19.78718610131657 40.19179175234087 24.534169265832027 3 19.450558237547583 24.04383192538848 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22.702621362630907 48-49 22.661064329719487 26.64904825692111 27.83647555425477 22.85341185910463 50-51 22.653522263123392 26.97901265695124 27.687418687912864 22.680046392012507 52-53 22.877057165967077 26.91163389253419 27.51381193181474 22.697497009683985 54-55 22.44281054485829 26.92001635468445 27.755158190423153 22.88201491003411 56-57 22.59120014975447 27.06275486903746 27.647390350841803 22.69865463036627 58-59 22.687598795133088 26.560912671355847 27.687389000465583 23.064099533045486 60-61 22.449127807280796 26.78441019566135 27.971939094585117 22.79452290247274 62-63 22.358035653412585 26.91603104555454 27.888373004939066 22.83756029609381 64-65 22.17280190410876 26.783669846904818 27.886358593702727 23.1571696552837 66-67 22.28218795775253 26.89457859637417 27.68918958715487 23.13404385871843 68-69 22.532086730236674 26.6080076585769 27.842602205633032 23.017303405553395 70-71 22.43055195487085 26.712059167751246 27.624293898724112 23.233094978653796 72-73 22.560425857366592 26.624639357388887 27.63739421805742 23.177540567187105 74-75 22.420537729598692 26.968228003676646 27.45415692562701 23.15707734109765 76-77 22.524546046911347 26.641745729784688 27.480517489373636 23.353190733930333 78-79 22.61139475832323 26.779943124436624 27.511773774108526 23.096888343131617 80-81 22.655357477103006 26.934283690369387 27.282599575300292 23.127759257227318 82-83 22.686349266027324 26.837493298457748 27.278796663206805 23.197360772308116 84-85 22.73099133297162 26.664212655838092 27.310881487412942 23.29391452377735 86-87 22.772443590974557 26.744312703477934 27.14993726727369 23.33330643827382 88-89 22.689226360210586 26.692718500373164 27.126633258498877 23.491421880917372 90-91 22.738740105626075 26.66106039280503 27.14250319589892 23.457696305669977 92-93 22.9637031571884 26.230804350593555 27.293213954485857 23.512278537732183 94-95 22.686669320791015 26.336850350770174 27.31602448762654 23.66045584081227 96-97 22.8731488140061 26.17023526173867 27.22727467165132 23.72934125260391 98-99 22.88301174230083 26.14505337547018 26.914894797417322 24.05704008481167 100-101 23.571638053374386 25.594971256856784 27.138266417379786 23.69512427238904 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 702.0 1 476.0 2 338.0 3 427.5 4 365.5 5 364.5 6 645.5 7 1311.0 8 2066.5 9 2348.0 10 2069.5 11 1846.0 12 1904.0 13 1999.5 14 2195.0 15 2391.0 16 2567.0 17 2748.0 18 2914.0 19 3276.0 20 3753.5 21 4391.0 22 5254.5 23 6206.5 24 7480.5 25 9323.0 26 11670.0 27 14779.0 28 18237.5 29 22161.0 30 26482.5 31 31230.0 32 36674.0 33 39999.5 34 42706.5 35 46755.5 36 51203.5 37 55638.0 38 60100.5 39 64045.0 40 68102.5 41 74111.0 42 79639.5 43 84729.0 44 89533.0 45 93276.5 46 95318.5 47 97292.0 48 101145.5 49 101885.5 50 98972.5 51 94972.0 52 90721.0 53 88008.0 54 85654.5 55 79952.0 56 72322.0 57 66042.5 58 60475.5 59 53235.0 60 44309.5 61 36629.0 62 29967.5 63 23419.5 64 18157.5 65 14242.0 66 10767.0 67 8185.5 68 6357.5 69 4777.0 70 3714.0 71 2897.0 72 2334.5 73 1951.0 74 1523.5 75 1225.5 76 894.5 77 579.5 78 398.5 79 250.0 80 144.5 81 94.5 82 57.0 83 31.5 84 19.5 85 11.0 86 8.0 87 7.5 88 6.0 89 4.0 90 2.5 91 2.0 92 1.5 93 1.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007422168079836713 2 0.10584657087767137 3 0.11593103837744953 4 0.1197227981573661 5 0.1614724936064476 6 0.09834372705783644 7 0.027187724379401867 8 0.02593725040942938 9 0.021903463409518122 10-11 0.051894669753858313 12-13 0.05502085467878954 14-15 0.02785329923438723 16-17 0.03283502617927763 18-19 0.03729236081417957 20-21 0.060365622453671955 22-23 0.0317862415593007 24-25 0.0515316289238663 26-27 0.05913531741869902 28-29 0.055283050833783764 30-31 0.06938113639847361 32-33 0.05221737271385122 34-35 0.06312876654861116 36-37 0.05324598839882859 38-39 0.03142320072930869 40-41 0.03709067146418401 42-43 0.033379587424265646 44-45 0.007119634054843367 46-47 0.012545077569724008 48-49 0.008612135244810533 50-51 0.007462505949835825 52-53 0.009076020749800328 54-55 0.010467677264769711 56-57 0.013553524319701823 58-59 0.017990690019604204 60-61 0.023960694779472866 62-63 0.010124805369777255 64-65 0.00847095269981364 66-67 0.009560075189789678 68-69 0.010911393834759949 70-71 0.030273571434333978 72-73 0.027086879704404092 74-75 0.028801239179366375 76-77 0.022387517849507474 78-79 0.005627132864876203 80-81 0.005324598839882858 82-83 0.006171694109864223 84-85 0.005042233749889071 86-87 0.011697982299742645 88-89 0.03725202294418046 90-91 0.03565867707921551 92-93 0.029507151904350843 94-95 0.04661040878397457 96-97 0.04251611497906464 98-99 0.025312013424443135 100-101 0.07504860713334893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2479060.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.12481152910115 #Duplication Level Percentage of deduplicated Percentage of total 1 75.90585620531145 42.60201873478159 2 13.314661172505632 14.94565697761439 3 4.709625582812662 7.929805446239816 4 2.1612080265432505 4.8518957265968226 5 1.1661164379943703 3.27240326517104 6 0.6707364456824091 2.2586973959774643 7 0.41793213674037627 1.6419453684555705 8 0.30202657808412314 1.356094781740061 9 0.2166134874217151 1.0941652040584575 >10 1.021378311020972 10.109222852608486 >50 0.06557380230334642 2.519807742575114 >100 0.04358222663088719 4.499050346352167 >500 0.0031023421353577264 1.1742866655172008 >1k 0.0014429498303957823 1.1906861861887952 >5k 1.4429498303957823E-4 0.5542633061231841 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7780 0.3138286285930958 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5908 0.23831613595475704 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4243 0.17115358240623463 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.0337869999112564E-5 0.0 0.0 0.0 6 0.0 4.0337869999112564E-5 0.0 0.0 0.0 7 0.0 4.0337869999112564E-5 0.0 0.0 0.0 8 0.0 4.0337869999112564E-5 0.0 0.0 0.0 9 0.0 4.0337869999112564E-5 0.0 0.0 0.0 10-11 0.0 4.0337869999112564E-5 0.0 4.0337869999112564E-5 4.0337869999112564E-5 12-13 0.0 4.0337869999112564E-5 0.0 4.0337869999112564E-5 1.4118254499689397E-4 14-15 0.0 4.0337869999112564E-5 0.0 4.0337869999112564E-5 1.6135147999645025E-4 16-17 0.0 8.067573999822513E-5 0.0 6.0506804998668846E-5 1.6135147999645025E-4 18-19 0.0 1.210136099973377E-4 0.0 8.067573999822513E-5 1.6135147999645025E-4 20-21 4.0337869999112564E-5 1.210136099973377E-4 0.0 1.2101360999733769E-4 1.8152041499600654E-4 22-23 4.0337869999112564E-5 1.210136099973377E-4 0.0 3.0253402499334423E-4 2.0168934999556285E-4 24-25 4.0337869999112564E-5 1.210136099973377E-4 0.0 8.874331399804765E-4 2.0168934999556285E-4 26-27 4.0337869999112564E-5 1.210136099973377E-4 0.0 0.0015328390599662776 2.2185828499511913E-4 28-29 4.0337869999112564E-5 1.210136099973377E-4 0.0 0.003953111259913032 2.420272199946754E-4 30-31 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.01254507756972401 2.420272199946754E-4 32-33 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.03005171314933886 2.420272199946754E-4 34-35 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.057602478358732744 2.420272199946754E-4 36-37 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.10465660371269755 2.420272199946754E-4 38-39 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.17692189781610773 2.420272199946754E-4 40-41 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.2560042919493679 2.420272199946754E-4 42-43 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.34041128492251094 3.0253402499334423E-4 44-45 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.4410139327002977 3.227029599929005E-4 46-47 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.5656176131275564 3.227029599929005E-4 48-49 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.6965745080796754 3.227029599929005E-4 50-51 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.8401571563415166 3.227029599929005E-4 52-53 4.0337869999112564E-5 1.6135147999645025E-4 0.0 0.979726186538446 3.428718949924568E-4 54-55 4.0337869999112564E-5 2.420272199946754E-4 0.0 1.1374875961049753 3.6304082999201313E-4 56-57 4.0337869999112564E-5 2.8236508999378795E-4 0.0 1.323546021475882 3.6304082999201313E-4 58-59 4.0337869999112564E-5 2.8236508999378795E-4 0.0 1.523137802231491 3.6304082999201313E-4 60-61 4.0337869999112564E-5 2.8236508999378795E-4 0.0 1.736283107306802 3.6304082999201313E-4 62-63 4.0337869999112564E-5 2.8236508999378795E-4 0.0 1.9533008479020275 3.6304082999201313E-4 64-65 4.0337869999112564E-5 3.227029599929005E-4 0.0 2.175320484377143 3.6304082999201313E-4 66-67 4.0337869999112564E-5 3.227029599929005E-4 0.0 2.435761942026413 3.6304082999201313E-4 68-69 4.0337869999112564E-5 3.227029599929005E-4 0.0 2.721575919905125 4.033786999911257E-4 70-71 4.0337869999112564E-5 3.227029599929005E-4 0.0 3.030725355578324 4.033786999911257E-4 72-73 4.0337869999112564E-5 3.227029599929005E-4 0.0 3.3420126983614757 4.2354763499068197E-4 74-75 4.0337869999112564E-5 3.227029599929005E-4 0.0 3.6582615991545184 4.840544399893508E-4 76-77 4.0337869999112564E-5 3.227029599929005E-4 0.0 3.998874573427025 4.840544399893508E-4 78-79 4.0337869999112564E-5 3.227029599929005E-4 0.0 4.375307576258743 4.840544399893508E-4 80-81 4.0337869999112564E-5 3.6304082999201313E-4 0.0 4.785745403499714 5.243923099884633E-4 82-83 4.0337869999112564E-5 3.6304082999201313E-4 0.0 5.199490936080611 5.647301799875759E-4 84-85 4.0337869999112564E-5 3.6304082999201313E-4 0.0 5.618520729631393 5.647301799875759E-4 86-87 4.0337869999112564E-5 3.6304082999201313E-4 0.0 6.050337627971892 5.848991149871322E-4 88-89 4.0337869999112564E-5 3.6304082999201313E-4 0.0 6.512327253071729 6.050680499866885E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6155 0.0 37.24494 1 GTATCAA 9745 0.0 34.407696 1 ATCAACG 12000 0.0 27.940786 3 TCAACGC 12125 0.0 27.57429 4 TATCAAC 12095 0.0 27.524721 2 CAACGCA 12255 0.0 27.242973 5 AACGCAG 12685 0.0 26.334625 6 GTACATG 9745 0.0 26.25722 1 TACATGG 9390 0.0 26.13454 2 ACATGGG 9980 0.0 23.922352 3 ACGCAGA 14185 0.0 23.352634 7 CGCAGAG 14405 0.0 22.995981 8 GAGTACT 7480 0.0 22.394066 12-13 GCAGAGT 15135 0.0 21.13234 9 GTACTTT 7995 0.0 20.917395 14-15 GGACCGA 845 0.0 20.806896 6 AGAGTAC 12155 0.0 20.370106 10-11 CATGGGG 5025 0.0 19.686022 4 GAGTACA 7435 0.0 19.638382 1 AGTACTT 7805 0.0 19.48285 12-13 >>END_MODULE