FastQCFastQC Report
Thu 26 May 2016
SRR938639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479060
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77350.31201342444313573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65640.2647777786741749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.1790598049260607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65500.037.8780981
GTATCAA100950.032.7218171
ATCAACG125100.026.1269383
TATCAAC127800.025.8099752
TCAACGC127150.025.6683374
CAACGCA129050.025.3272345
GTACATG99400.024.529571
AACGCAG133850.024.442636
TACATGG98400.024.0060252
ACATGGG101750.022.597913
ACGCAGA150100.021.6382687
GAGTACT77850.021.59194412-13
GGACCGA7950.021.5021786
CGCAGAG151300.021.4666488
GTACTTT81100.020.7266714-15
AGTACTT79450.020.43992412-13
CATGGGA54850.020.1807174
AGAGTAC131550.019.83462110-11
GCAGAGT162200.019.6727719
GTAAGGT9650.018.7074094
Produced by FastQC (version 0.11.5)