FastQCFastQC Report
Thu 26 May 2016
SRR938638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.3168207345103407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59750.24362984410544217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.16505667095210544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65800.035.2327841
GTATCAA100900.033.8276521
ATCAACG126900.026.5142463
GTACATG99500.026.4573271
TATCAAC127650.026.4329222
CAACGCA128450.026.266695
TACATGG97350.026.2147662
TCAACGC128400.026.20454
AACGCAG132700.025.4923826
ACATGGG102400.024.1329863
ACGCAGA148000.022.6309177
CGCAGAG149800.022.2321248
GAGTACT76550.021.34559412-13
AGTACTT77800.020.94158412-13
AGAGTAC126700.020.64133510-11
GTACTTT81250.020.14232414-15
GCAGAGT157950.020.0919789
CCGATCG2401.4123361E-719.7949629
CATGGGG50750.019.6648644
CATGGGA55750.019.1798534
Produced by FastQC (version 0.11.5)