FastQCFastQC Report
Thu 26 May 2016
SRR938638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76920.31364029470444543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.2626309332022013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45910.18719742498545358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66000.034.37111
GTATCAA102150.032.7757951
ATCAACG124050.026.7879713
TATCAAC126300.026.3580762
TCAACGC126400.026.2899364
CAACGCA127950.025.9714555
GTACATG97600.025.484191
AACGCAG131050.025.3338386
TACATGG95950.025.2780882
ACATGGG100650.023.4748083
ACGCAGA147900.022.3512527
CGCAGAG148450.022.0764738
GAGTACT76500.021.48203712-13
GTACTTT79550.020.38972314-15
AGTACTT78550.020.31673412-13
AGAGTAC125250.020.23100710-11
GCAGAGT161600.019.9567229
CATGGGA54250.019.54184
TAGCGCG2451.7425191E-719.404094
CATGGGG49150.019.1514244
Produced by FastQC (version 0.11.5)