##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938638_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2452491 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795459392103783 31.0 31.0 34.0 30.0 34.0 2 32.02877319427472 31.0 31.0 34.0 30.0 34.0 3 32.09555712946551 33.0 31.0 34.0 30.0 34.0 4 35.75780339255068 37.0 35.0 37.0 35.0 37.0 5 35.624131546252364 37.0 35.0 37.0 33.0 37.0 6 35.650690257375054 37.0 35.0 37.0 33.0 37.0 7 35.59254447824681 37.0 35.0 37.0 33.0 37.0 8 35.58573099758572 37.0 35.0 37.0 33.0 37.0 9 37.169337624480576 39.0 37.0 39.0 33.0 39.0 10-11 37.0796219027919 39.0 37.0 39.0 33.0 39.0 12-13 36.971794595780366 39.0 37.0 39.0 33.0 39.0 14-15 38.02885046265205 40.0 37.0 41.0 33.0 41.0 16-17 37.957934198331415 40.0 37.0 41.0 33.0 41.0 18-19 38.0713892528046 40.0 37.0 41.0 33.0 41.0 20-21 38.12439250541592 40.0 37.0 41.0 33.0 41.0 22-23 38.027725484007895 40.0 37.0 41.0 33.0 41.0 24-25 37.93404297915874 40.0 37.0 41.0 33.0 41.0 26-27 37.74557419374831 40.0 37.0 41.0 32.0 41.0 28-29 37.62642696751996 40.0 37.0 41.0 32.0 41.0 30-31 37.40527692048615 39.0 36.0 41.0 31.5 41.0 32-33 37.20134528526303 39.0 36.0 41.0 31.0 41.0 34-35 37.045924939174085 39.0 36.0 41.0 31.0 41.0 36-37 37.0368515113817 39.0 36.0 41.0 31.0 41.0 38-39 36.918794401284245 39.0 36.0 41.0 31.0 41.0 40-41 36.75290266100875 39.0 35.0 41.0 30.0 41.0 42-43 36.5481284131114 39.0 35.0 41.0 30.0 41.0 44-45 36.28758474546899 39.0 35.0 40.0 30.0 41.0 46-47 35.93182074878155 38.5 35.0 40.0 29.0 41.0 48-49 35.59200808484108 38.0 35.0 40.0 27.5 41.0 50-51 35.23448526416611 38.0 34.0 40.0 27.0 41.0 52-53 34.760878633193755 38.0 33.5 40.0 26.0 41.0 54-55 34.45871259058647 38.0 33.0 40.0 24.5 41.0 56-57 34.13176052429958 37.5 33.0 40.0 24.0 41.0 58-59 33.70511573742778 37.0 32.5 40.0 23.0 41.0 60-61 33.34025323640331 36.5 32.0 39.0 22.5 41.0 62-63 32.900019612712136 36.0 31.5 39.0 21.5 40.0 64-65 32.41130324229528 35.5 31.0 39.0 20.0 40.0 66-67 31.930367124690775 35.0 30.5 38.0 18.5 40.0 68-69 31.399192698362604 35.0 30.0 38.0 17.5 40.0 70-71 30.82665400199226 34.0 29.0 37.0 13.5 39.0 72-73 30.38785544982632 34.0 29.0 36.5 11.5 39.0 74-75 29.876720036893104 34.0 29.0 36.0 8.0 39.0 76-77 28.599116979430303 32.5 27.0 34.5 7.0 37.0 78-79 29.30147307370343 33.5 29.0 35.0 7.0 37.0 80-81 29.02687675510328 34.0 28.5 35.0 2.0 37.0 82-83 28.559807355052477 33.0 27.5 35.0 2.0 36.5 84-85 28.04610842608597 33.0 26.5 35.0 2.0 36.0 86-87 27.47466616595127 32.5 26.0 35.0 2.0 35.5 88-89 26.8447598788334 32.0 25.0 34.5 2.0 35.0 90-91 26.31314345292195 32.0 24.5 34.0 2.0 35.0 92-93 25.58249388071149 31.5 22.5 34.0 2.0 35.0 94-95 24.828105587339564 31.0 19.0 34.0 2.0 35.0 96-97 23.840153949596555 31.0 8.0 34.0 2.0 35.0 98-99 22.611736189857577 30.5 2.0 34.0 2.0 35.0 100-101 18.943798978263324 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2715183486425623 1101 2 0.37468208064303354 1101 3 0.31739040693777554 1101 4 0.24330771910651094 1101 5 0.10595157271135491 1101 6 0.1954298986566343 1101 7 0.31396197108477253 1101 8 0.2608495626958458 1101 9 0.2734035100237264 1101 10-11 0.40948171955796653 1101 12-13 0.3749972922182536 1101 14-15 0.451983699951505 1101 16-17 0.5092976272958438 1101 18-19 0.4514261423395851 1101 20-21 0.45488083008729063 1101 22-23 0.42231778595152036 1101 24-25 0.43824862855827007 1101 26-27 0.42178131244856587 1101 28-29 0.44087843833302287 1101 30-31 0.25816540785196196 1101 32-33 0.33542739437346825 1101 34-35 0.19223995872255273 1101 36-37 0.32295059883943367 1101 38-39 0.4278628968242657 1101 40-41 0.4755824545335443 1101 42-43 0.5061552397739817 1101 44-45 0.37535425044295323 1101 46-47 0.5565700206443722 1101 48-49 0.6421310987802755 1101 50-51 0.570321942667583 1101 52-53 0.7208431477670914 1101 54-55 0.5167312918442306 1101 56-57 0.5593425774056442 1101 58-59 0.737703440546035 1101 60-61 0.650505605323481 1101 62-63 0.5068247334838603 1101 64-65 0.7256860497702959 1101 66-67 0.7102275307856978 1101 68-69 0.5571396447595127 1101 70-71 0.5174100884316957 1101 72-73 0.4326498731552455 1101 74-75 0.519314062980655 1101 76-77 0.4783903121740707 1101 78-79 0.44981627864887486 1101 80-81 0.4781663460476082 1101 82-83 0.4731286493105138 1101 84-85 0.6825746215211659 1101 86-87 0.814527135512666 1101 88-89 0.7028899304586922 1101 90-91 0.8460949676942526 1101 92-93 0.9744747842415151 1101 94-95 1.012166508371795 1101 96-97 0.9210500930894803 1101 98-99 0.8541429704048618 1101 100-101 0.8150328127515216 1102 1 0.26558450810088274 1102 2 0.31647421359448913 1102 3 0.2824296290726238 1102 4 0.24878804752277262 1102 5 0.11265681612561451 1102 6 0.22162769838438123 1102 7 0.2638895856506167 1102 8 0.2642878257912358 1102 9 0.2116502155769311 1102 10-11 0.3242273835461802 1102 12-13 0.36667386783069134 1102 14-15 0.45951005606622886 1102 16-17 0.4160093906222997 1102 18-19 0.41392503017224413 1102 20-21 0.42135407269178415 1102 22-23 0.374342038584075 1102 24-25 0.46990955638140264 1102 26-27 0.2966383516663811 1102 28-29 0.33276640678961655 1102 30-31 0.36541211016321284 1102 32-33 0.2949818754442859 1102 34-35 0.3682898356907316 1102 36-37 0.4028920168879253 1102 38-39 0.49433493270468176 1102 40-41 0.584959385066945 1102 42-43 0.4310261293025306 1102 44-45 0.5266053171330114 1102 46-47 0.416104545740815 1102 48-49 0.5938902323307644 1102 50-51 0.500839040146758 1102 52-53 0.5118901376028688 1102 54-55 0.5462533462577568 1102 56-57 0.6157144227677307 1102 58-59 0.6191133517491494 1102 60-61 0.6165399245563989 1102 62-63 0.4957297160295795 1102 64-65 0.46018153644212134 1102 66-67 0.690869605256065 1102 68-69 0.3305509925046124 1102 70-71 0.38674330313384786 1102 72-73 0.14284121636882574 1102 74-75 0.14597983751200871 1102 76-77 0.23952612410666418 1102 78-79 0.3417959221141409 1102 80-81 0.37042578740280163 1102 82-83 0.3588138758738637 1102 84-85 0.1236623518028459 1102 86-87 0.038422901928345965 1102 88-89 0.3189961488339641 1102 90-91 0.12819445867281942 1102 92-93 0.09441316206574513 1102 94-95 0.2936722730908592 1102 96-97 0.09013915328916511 1102 98-99 -0.08464423947255639 1102 100-101 -0.3094274483835733 1103 1 0.2612073147218048 1103 2 0.24497275105192529 1103 3 0.25790969432185307 1103 4 0.24210730613020814 1103 5 0.1592738386602477 1103 6 0.1238015131336283 1103 7 0.21220913989686352 1103 8 0.21767428136020328 1103 9 0.2451631349927439 1103 10-11 0.3137640305847498 1103 12-13 0.3969302388469558 1103 14-15 0.4517973613359061 1103 16-17 0.42244822990956976 1103 18-19 0.43201447308439356 1103 20-21 0.36503574969421493 1103 22-23 0.46039345191147163 1103 24-25 0.5100790443697676 1103 26-27 0.4611190881895979 1103 28-29 0.4447936890342419 1103 30-31 0.34736944774447664 1103 32-33 0.46392623622102036 1103 34-35 0.3413613141977905 1103 36-37 0.4584660257153459 1103 38-39 0.3003960499411136 1103 40-41 0.4243256050291748 1103 42-43 0.4903530119130579 1103 44-45 0.5779174317156617 1103 46-47 0.5072732928931103 1103 48-49 0.5798339983517806 1103 50-51 0.5323463111223248 1103 52-53 0.4475318303263265 1103 54-55 0.6525108024423503 1103 56-57 0.6407672029898421 1103 58-59 0.698155342893898 1103 60-61 0.6526014243842297 1103 62-63 0.5007150558229156 1103 64-65 0.5053753189841288 1103 66-67 0.4525047313199302 1103 68-69 0.45225418505590653 1103 70-71 0.41014172337919064 1103 72-73 0.31667387784848344 1103 74-75 0.6671383590397291 1103 76-77 0.6074240974156986 1103 78-79 0.653954154959262 1103 80-81 0.5292478232759592 1103 82-83 0.5589105671539087 1103 84-85 0.3999677710360636 1103 86-87 0.4308705477739281 1103 88-89 0.4655170018040913 1103 90-91 0.6217677927481482 1103 92-93 0.589837021160811 1103 94-95 0.5427090122991736 1103 96-97 0.5295672834954495 1103 98-99 0.44745796112359315 1103 100-101 0.16716474049136565 1104 1 0.3100209314548046 1104 2 0.3110935064080138 1104 3 0.2994712151957586 1104 4 0.1548010143188847 1104 5 0.07874881537292566 1104 6 0.19821424918240638 1104 7 0.11335911025845036 1104 8 0.23929338543337053 1104 9 0.29629362868399767 1104 10-11 0.3965085474786392 1104 12-13 0.330530035612135 1104 14-15 0.44873444322259814 1104 16-17 0.46827344796360393 1104 18-19 0.3242331198792101 1104 20-21 0.43818165535938647 1104 22-23 0.4097939091898155 1104 24-25 0.35385074392096527 1104 26-27 0.4315619707368157 1104 28-29 0.44036994443266764 1104 30-31 0.4183559834375927 1104 32-33 0.4298454503797089 1104 34-35 0.5630269929174148 1104 36-37 0.5942480563699348 1104 38-39 0.6221387885303287 1104 40-41 0.6493785307637836 1104 42-43 0.6593012717149023 1104 44-45 0.5724961428681681 1104 46-47 0.47157848384916434 1104 48-49 0.53790326755324 1104 50-51 0.7282485890459469 1104 52-53 0.7557230404203281 1104 54-55 0.8067490952698577 1104 56-57 0.8197984503435549 1104 58-59 0.9125731598132063 1104 60-61 0.7933787975741708 1104 62-63 0.7509157502085557 1104 64-65 0.6837646830284996 1104 66-67 0.5530907250535257 1104 68-69 0.41285542140106557 1104 70-71 0.5405693633148339 1104 72-73 0.4669219936923277 1104 74-75 0.44889634024050906 1104 76-77 0.5364748214397466 1104 78-79 0.5085184703119623 1104 80-81 0.678941277421103 1104 82-83 0.6157341762656046 1104 84-85 0.5767464991248055 1104 86-87 0.5445304681736225 1104 88-89 0.28735288968621475 1104 90-91 0.5246698166282364 1104 92-93 0.5322732985763423 1104 94-95 0.5734584407012413 1104 96-97 0.4668295410955885 1104 98-99 0.4991558186898537 1104 100-101 0.47406879671966706 1105 1 0.25699372057045267 1105 2 0.2555833023263787 1105 3 0.1856957049916801 1105 4 0.14459693268622686 1105 5 0.14298917818472034 1105 6 0.24864508818467357 1105 7 0.15288291978225743 1105 8 0.1910507550478826 1105 9 0.25150904818513453 1105 10-11 0.3009133093834109 1105 12-13 0.2747590605554464 1105 14-15 0.35705076975321504 1105 16-17 0.24491743889330309 1105 18-19 0.2514383352987082 1105 20-21 0.32478596374941304 1105 22-23 0.3119651110038717 1105 24-25 0.2643099275944323 1105 26-27 0.39519008865064364 1105 28-29 0.2325694909179319 1105 30-31 0.3573979335509705 1105 32-33 0.3266765161393508 1105 34-35 0.42155873894915885 1105 36-37 0.43940111759442146 1105 38-39 0.4970197409112842 1105 40-41 0.5066347665914464 1105 42-43 0.4350609089031181 1105 44-45 0.4722523786958277 1105 46-47 0.06957735006231758 1105 48-49 0.13102118138544228 1105 50-51 -1.8678510371472612E-4 1105 52-53 0.02424344858358296 1105 54-55 0.29107449209524816 1105 56-57 0.1637723732460472 1105 58-59 0.15049266094698766 1105 60-61 0.15715997671249227 1105 62-63 0.199118698054356 1105 64-65 0.12517624765435187 1105 66-67 0.018158752264408662 1105 68-69 0.07142118103825723 1105 70-71 -0.08390342580081267 1105 72-73 -0.039557598144405404 1105 74-75 0.06866391393665339 1105 76-77 0.02189999150777666 1105 78-79 0.07855815285164525 1105 80-81 0.13904331823743377 1105 82-83 0.18122170461027665 1105 84-85 0.14949026329714243 1105 86-87 0.10702479923938313 1105 88-89 -0.04425708763804792 1105 90-91 0.0626119934989866 1105 92-93 0.3110828223858668 1105 94-95 0.43246070827493455 1105 96-97 0.3350948472180342 1105 98-99 0.14549935610481768 1105 100-101 -0.2135332440966593 1106 1 0.12752955376109654 1106 2 0.10171080646426844 1106 3 0.0749471746566499 1106 4 0.09637479924635528 1106 5 -0.008021672286780301 1106 6 -0.03335048538101404 1106 7 0.06245904747664355 1106 8 0.048486435406054795 1106 9 -0.0032978819114504176 1106 10-11 0.044415630407705464 1106 12-13 -0.03380574104770773 1106 14-15 0.02503561650853925 1106 16-17 0.057124377868120746 1106 18-19 0.28286863481230284 1106 20-21 0.043614401370298594 1106 22-23 0.23116247099728326 1106 24-25 0.191345342239714 1106 26-27 0.075156351357613 1106 28-29 0.18786250438113683 1106 30-31 0.09109979290704473 1106 32-33 0.017219214108131098 1106 34-35 -0.0888621686601283 1106 36-37 -0.362594344993326 1106 38-39 -0.15657475521717146 1106 40-41 0.022386023812941858 1106 42-43 0.055608315507249984 1106 44-45 0.12198214741781044 1106 46-47 -0.15815881098886564 1106 48-49 0.022643456877069923 1106 50-51 0.04033506486679528 1106 52-53 0.09771028837584339 1106 54-55 -0.3093629987886999 1106 56-57 0.15730006065607682 1106 58-59 0.11947076024370062 1106 60-61 -0.186550348674821 1106 62-63 -0.10424670790293789 1106 64-65 -0.5643009035736988 1106 66-67 -0.20505101506241985 1106 68-69 -0.9282692697858366 1106 70-71 -0.6537722023166097 1106 72-73 -0.4143885153294953 1106 74-75 -0.3122608018012869 1106 76-77 -0.02169888032222289 1106 78-79 -0.2235630318854014 1106 80-81 -0.9719791690635233 1106 82-83 -0.30863078985668224 1106 84-85 -0.24669590060775803 1106 86-87 -0.08262412998206159 1106 88-89 0.08068187449627828 1106 90-91 0.05520492959471923 1106 92-93 -0.12617770307881315 1106 94-95 -0.13211862192564894 1106 96-97 -0.4175091708174641 1106 98-99 -0.4875964051721233 1106 100-101 -0.7401406009826843 1107 1 0.1563507361039349 1107 2 0.18339078895739647 1107 3 0.13426331385340262 1107 4 0.0910446026633096 1107 5 0.01882739225822405 1107 6 0.13121872369948306 1107 7 0.06883657397120402 1107 8 0.0310537259332051 1107 9 0.11949563675995734 1107 10-11 0.1250570577918566 1107 12-13 0.16857325289904423 1107 14-15 0.2648907239167002 1107 16-17 0.11890097170036285 1107 18-19 0.18433134098583537 1107 20-21 0.15116212027263032 1107 22-23 0.1768800163599593 1107 24-25 0.218884029962652 1107 26-27 0.16900385658205863 1107 28-29 0.142937880698085 1107 30-31 0.07142157330989818 1107 32-33 0.12080583453337823 1107 34-35 0.20015457122146074 1107 36-37 0.040155510260582616 1107 38-39 0.03611128662046781 1107 40-41 0.0284028677713124 1107 42-43 0.025991220421666128 1107 44-45 -0.010610918138056036 1107 46-47 0.05224966141110343 1107 48-49 0.12567146574161114 1107 50-51 -0.12342335245793379 1107 52-53 0.1704714852528042 1107 54-55 0.027069950884829552 1107 56-57 0.08635852892044227 1107 58-59 -0.08508262977535708 1107 60-61 -0.06542617896189995 1107 62-63 0.04531278174844289 1107 64-65 0.022518366319999927 1107 66-67 0.07292702320915012 1107 68-69 0.289213601036554 1107 70-71 -0.12964126639159446 1107 72-73 0.035910316294096134 1107 74-75 0.04326390544840919 1107 76-77 0.04967246413318804 1107 78-79 0.1949585008696424 1107 80-81 0.19211818454758856 1107 82-83 0.12431651829332324 1107 84-85 0.1789826663189551 1107 86-87 0.17671752481446035 1107 88-89 0.10443572281947056 1107 90-91 0.10410995632047815 1107 92-93 -0.06981226292098341 1107 94-95 -0.17415587775341734 1107 96-97 -0.06836199006233201 1107 98-99 -0.31903145622010953 1107 100-101 -0.5330042143029239 1108 1 0.210449414882671 1108 2 0.22780708011660522 1108 3 0.19873120927571364 1108 4 0.21402068807627472 1108 5 0.1897386632917062 1108 6 0.15117195284403806 1108 7 0.12775458542225238 1108 8 0.13656216358359075 1108 9 0.21342096570269575 1108 10-11 0.26197643122171854 1108 12-13 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-0.7953385485310847 2316 58-59 -0.8576172738103907 2316 60-61 -0.7362041001136461 2316 62-63 -0.8181470865022007 2316 64-65 -0.5948502435835188 2316 66-67 -0.66385188147677 2316 68-69 -0.7107147700701226 2316 70-71 -0.6922193000582197 2316 72-73 -0.6383529219055113 2316 74-75 -0.31750050555295317 2316 76-77 -0.5285788527479589 2316 78-79 -0.36031219061881714 2316 80-81 -0.5164835268377104 2316 82-83 -0.27438506423466436 2316 84-85 -0.22234491061077222 2316 86-87 -0.1803621435445102 2316 88-89 0.051840770094536026 2316 90-91 0.019799313088537218 2316 92-93 -0.08419577790837707 2316 94-95 -0.2611196283050141 2316 96-97 0.049820530217086656 2316 98-99 0.10522080236811249 2316 100-101 0.301883119946023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 40.0 5 79.0 6 195.0 7 425.0 8 1025.0 9 1879.0 10 3016.0 11 4742.0 12 6485.0 13 9049.0 14 11997.0 15 15572.0 16 19630.0 17 24046.0 18 23170.0 19 19882.0 20 19779.0 21 21142.0 22 22860.0 23 26176.0 24 29895.0 25 35805.0 26 43045.0 27 51689.0 28 62123.0 29 74004.0 30 88540.0 31 105317.0 32 126897.0 33 156301.0 34 199685.0 35 260423.0 36 347877.0 37 410790.0 38 218749.0 39 10143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.453964966075155 19.313755871607516 14.983404553757829 23.2488746085595 2 15.867781137035553 20.57391498036323 39.35736959101592 24.2009342915853 3 19.432547132113445 24.221967422204898 29.797616115684285 26.547869329997376 4 13.389162284387588 16.887156772440758 36.28881818526551 33.434862757906146 5 13.909189115949427 37.35524737573057 33.993619559392904 14.74194394892709 6 32.5773672563936 35.994668278089506 17.012947244250846 14.415017221266051 7 29.031299197428247 32.45010888928848 20.178871196673096 18.339720716610174 8 26.493349007193096 35.28567485059069 19.373975276565744 18.847000865650475 9 26.36604986521867 16.875332060341915 19.90331462990078 36.855303444538634 10-11 25.19640235173136 26.66876249494901 26.9727187581932 21.162116395126425 12-13 26.06474396847939 23.818721455043057 27.49102850938087 22.62550606709668 14-15 22.662713135338723 25.320113305206824 25.924845391889306 26.092328167565142 16-17 22.371682505664648 28.577699163829756 26.516386808351182 22.534231522154414 18-19 22.29951098699241 27.088356287546013 28.855233311763428 21.756899413698154 20-21 22.94844303200297 26.14876058668513 29.006854663279093 21.89594171803281 22-23 22.594027867992175 26.11695618862618 28.844448358831897 22.44456758454975 24-25 22.572599337528942 26.2932752480189 28.932517208569514 22.201608205882646 26-27 22.209132240217887 26.669370298463825 28.834049612255157 22.287447849063128 28-29 22.1385929652749 26.916062077292025 28.85621190862678 22.089133048806296 30-31 22.229092787700342 26.494368378925753 28.781186149103092 22.49535268427081 32-33 22.070070797405577 26.539934295375602 28.65441708042965 22.73557782678917 34-35 22.733261751732005 26.6304150577458 28.489042346647704 22.14728084387449 36-37 22.75621272026352 26.253395275859603 28.60300873989827 22.38738326397861 38-39 22.303492612878287 26.622813956750512 28.54441977902247 22.52927365134873 40-41 22.603582917509225 26.439359000864066 28.526512635384947 22.430545446241762 42-43 22.479651193609076 26.417831271065428 28.282720578940975 22.819796956384522 44-45 22.40761430219899 26.60369665115862 28.37881092277644 22.609878123865947 46-47 22.576882035448858 26.88132188864302 27.856819861928138 22.68497621397999 48-49 22.58269245432501 26.710780997769206 27.92222275229552 22.78430379561026 50-51 22.60273330258908 27.00268828713337 27.791743170515204 22.60283523976235 52-53 22.893743544828503 26.946296642882682 27.487950006748243 22.672009805540572 54-55 22.447442212835846 26.972045972849646 27.7544749399692 22.82603687434531 56-57 22.58660684177842 27.05247440255642 27.64774671955983 22.71317203610533 58-59 22.620286883825464 26.659037688619446 27.664158604455636 23.05651682309945 60-61 22.434108830572672 26.841403291592098 27.981448249962998 22.743039627872232 62-63 22.297329531484518 26.990374276602854 27.87898100339614 22.833315188516494 64-65 22.103281928455598 26.92476751188893 27.765769578767056 23.206180980888412 66-67 22.161685095899493 26.941603623583305 27.643869627168637 23.252841653348565 68-69 22.42069818704928 26.760146725785393 27.715185450073516 23.103969637091808 70-71 22.286431341208324 26.86469669601099 27.50376148322725 23.345110479553433 72-73 22.443058914385414 26.785113584514686 27.51749139956069 23.25433610153921 74-75 22.244811499817942 27.18456459167434 27.267602613016724 23.303021295490993 76-77 22.266605667462184 26.832065846520948 27.473291441232604 23.42803704478426 78-79 22.348746641679824 26.952759459667742 27.5246270016893 23.173866896963126 80-81 22.30462034792877 27.18144586487994 27.3283190401785 23.18561474701279 82-83 22.33417696950718 27.09484974183265 27.29163022394891 23.27934306471126 84-85 22.30806870890463 26.972118864366173 27.279270095315766 23.440542331413425 86-87 22.32972733498434 27.041032375288054 27.134021341589598 23.495218948138007 88-89 22.180364519473887 27.085544522319978 27.047419988428086 23.68667096977805 90-91 22.150499313636164 27.03362173594901 27.171118772035662 23.644760178379162 92-93 22.292774690979847 26.74459356171402 27.253937306865588 23.708694440440546 94-95 21.986973639223997 26.79568120299035 27.2902808718573 23.927064285928353 96-97 22.053643021247176 26.72825847847321 27.25203216893195 23.966066331347665 98-99 21.989220742364488 26.803495062972786 26.91776677398625 24.289517420676475 100-101 21.946608967496513 26.6997160641397 27.17782189166482 24.17585307669897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 700.0 1 460.5 2 299.5 3 402.5 4 365.0 5 362.5 6 656.0 7 1403.0 8 2169.5 9 2404.0 10 2108.0 11 1789.5 12 1839.0 13 2039.5 14 2235.5 15 2458.0 16 2688.5 17 2862.0 18 3047.5 19 3396.5 20 3856.0 21 4387.0 22 5204.5 23 6222.5 24 7526.0 25 9300.0 26 11687.5 27 14854.0 28 18115.0 29 22020.5 30 26656.5 31 31368.5 32 36871.5 33 40172.0 34 42405.5 35 46388.0 36 50681.0 37 55320.5 38 60019.0 39 63535.5 40 67471.0 41 74152.5 42 80014.5 43 85214.0 44 90076.0 45 93952.0 46 95269.0 47 97714.5 48 102827.0 49 102631.5 50 99414.0 51 95221.0 52 89774.5 53 86395.0 54 85059.0 55 79699.5 56 70775.0 57 64032.0 58 58596.5 59 51155.0 60 41988.0 61 34081.0 62 27746.0 63 21221.5 64 15856.5 65 12241.5 66 9221.5 67 7251.0 68 5584.0 69 4028.0 70 3086.0 71 2456.0 72 1995.0 73 1750.0 74 1473.0 75 1188.5 76 793.5 77 443.5 78 292.5 79 185.0 80 107.0 81 59.5 82 29.0 83 18.0 84 12.0 85 7.0 86 3.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.485235623698517E-4 2 0.04982689029235989 3 9.378219940460535E-4 4 0.0 5 0.08929696378090685 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.6503665049127604E-4 26-27 0.009541319417685937 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011335413667165343 36-37 1.0193717326587539E-4 38-39 0.004689109970230268 40-41 0.005382282748438221 42-43 5.300733009825521E-4 44-45 4.077486930635016E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.116230395952523E-5 68-69 0.001019371732658754 70-71 4.077486930635016E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014271204257222554 82-83 4.892984316762018E-4 84-85 0.004729884839536618 86-87 0.002018356030664333 88-89 8.154973861270031E-5 90-91 0.0030377277633230866 92-93 8.766596900865283E-4 94-95 1.0193717326587539E-4 96-97 2.038743465317508E-5 98-99 1.2232460791905046E-4 100-101 2.038743465317508E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2452491.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.32273885030329 #Duplication Level Percentage of deduplicated Percentage of total 1 73.51205856467271 39.19864301192244 2 14.559402713077931 15.526944573717035 3 5.185037397903222 8.294411852923487 4 2.3943732625586827 5.106981607582613 5 1.2346404058702827 3.291720396812678 6 0.77091439877207 2.4664360296996994 7 0.4866372725597065 1.8164182539667566 8 0.3347060774112833 1.4277955805929003 9 0.23753836745064755 1.1399576701048435 >10 1.1553366179016296 10.891000651649547 >50 0.07537467974790855 2.7919093652777582 >100 0.04861985111568186 4.853255132612968 >500 0.003445965616024415 1.221844692869623 >1k 0.0017612713148541285 1.3955889420461454 >5k 1.5315402737861987E-4 0.5770922382216096 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7692 0.31364029470444543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6441 0.2626309332022013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4591 0.18719742498545358 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.077486930635016E-5 0.0 0.0 0.0 6 0.0 4.077486930635016E-5 0.0 0.0 0.0 7 0.0 4.077486930635016E-5 0.0 0.0 0.0 8 0.0 4.077486930635016E-5 0.0 0.0 0.0 9 0.0 4.077486930635016E-5 0.0 0.0 0.0 10-11 0.0 4.077486930635016E-5 0.0 0.0 4.077486930635016E-5 12-13 0.0 8.154973861270031E-5 0.0 0.0 8.154973861270031E-5 14-15 0.0 1.0193717326587539E-4 0.0 0.0 8.154973861270031E-5 16-17 0.0 1.2232460791905046E-4 0.0 6.116230395952524E-5 8.154973861270031E-5 18-19 0.0 1.2232460791905046E-4 0.0 1.2232460791905046E-4 8.154973861270031E-5 20-21 0.0 1.2232460791905046E-4 0.0 2.0387434653175077E-4 8.154973861270031E-5 22-23 0.0 1.2232460791905046E-4 0.0 4.4852356236985174E-4 8.154973861270031E-5 24-25 0.0 1.2232460791905046E-4 0.0 8.358848207801783E-4 8.154973861270031E-5 26-27 0.0 1.2232460791905046E-4 0.0 0.001855256553438932 8.154973861270031E-5 28-29 0.0 1.2232460791905046E-4 0.0 0.004872596882108844 8.154973861270031E-5 30-31 0.0 1.2232460791905046E-4 0.0 0.013476094305748726 8.154973861270031E-5 32-33 0.0 1.2232460791905046E-4 0.0 0.031824785493606296 8.154973861270031E-5 34-35 0.0 1.2232460791905046E-4 0.0 0.061590440087241904 8.154973861270031E-5 36-37 0.0 1.2232460791905046E-4 0.0 0.11176391676870578 8.154973861270031E-5 38-39 0.0 1.2232460791905046E-4 0.0 0.18556643021319957 8.154973861270031E-5 40-41 0.0 1.2232460791905046E-4 0.0 0.2680947656892523 8.154973861270031E-5 42-43 0.0 1.2232460791905046E-4 0.0 0.35048038912273277 8.154973861270031E-5 44-45 0.0 1.2232460791905046E-4 0.0 0.4566377613618154 8.154973861270031E-5 46-47 0.0 1.2232460791905046E-4 0.0 0.5797574792323397 8.154973861270031E-5 48-49 0.0 1.2232460791905046E-4 0.0 0.7144368725512142 1.0193717326587539E-4 50-51 0.0 1.2232460791905046E-4 0.0 0.8529694910195389 1.2232460791905046E-4 52-53 0.0 1.2232460791905046E-4 0.0 0.9924195440472565 1.2232460791905046E-4 54-55 0.0 2.2426178118492587E-4 0.0 1.1517065709925134 1.2232460791905046E-4 56-57 0.0 3.2619895445080125E-4 0.0 1.3403515038383422 1.2232460791905046E-4 58-59 0.0 3.8736125841032647E-4 0.0 1.5436142273304978 1.2232460791905046E-4 60-61 0.0 4.0774869306350154E-4 0.0 1.7627995372867833 1.2232460791905046E-4 62-63 0.0 4.0774869306350154E-4 0.0 1.9814139990727795 1.2232460791905046E-4 64-65 0.0 4.0774869306350154E-4 0.0 2.2102425656200166 1.2232460791905046E-4 66-67 0.0 4.0774869306350154E-4 0.0 2.4761558758013793 1.2232460791905046E-4 68-69 0.0 4.4852356236985174E-4 0.0 2.764801175621032 1.2232460791905046E-4 70-71 0.0 4.4852356236985174E-4 0.0 3.079481229492789 1.2232460791905046E-4 72-73 0.0 4.4852356236985174E-4 0.0 3.402295869791163 1.4271204257222556E-4 74-75 0.0 4.4852356236985174E-4 0.0 3.7289841226736407 1.6309947722540063E-4 76-77 0.0 4.4852356236985174E-4 0.0 4.077405380896403 1.6309947722540063E-4 78-79 0.0 4.4852356236985174E-4 0.0 4.473410911599675 1.6309947722540063E-4 80-81 0.0 4.4852356236985174E-4 0.0 4.896062819394649 1.6309947722540063E-4 82-83 0.0 4.4852356236985174E-4 0.0 5.327481324090486 1.6309947722540063E-4 84-85 0.0 4.4852356236985174E-4 0.0 5.760000750257595 1.6309947722540063E-4 86-87 0.0 4.4852356236985174E-4 0.0 6.219370427862936 1.6309947722540063E-4 88-89 0.0 4.4852356236985174E-4 0.0 6.712012398822258 1.6309947722540063E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6600 0.0 34.3711 1 GTATCAA 10215 0.0 32.775795 1 ATCAACG 12405 0.0 26.787971 3 TATCAAC 12630 0.0 26.358076 2 TCAACGC 12640 0.0 26.289936 4 CAACGCA 12795 0.0 25.971455 5 GTACATG 9760 0.0 25.48419 1 AACGCAG 13105 0.0 25.333838 6 TACATGG 9595 0.0 25.278088 2 ACATGGG 10065 0.0 23.474808 3 ACGCAGA 14790 0.0 22.351252 7 CGCAGAG 14845 0.0 22.076473 8 GAGTACT 7650 0.0 21.482037 12-13 GTACTTT 7955 0.0 20.389723 14-15 AGTACTT 7855 0.0 20.316734 12-13 AGAGTAC 12525 0.0 20.231007 10-11 GCAGAGT 16160 0.0 19.956722 9 CATGGGA 5425 0.0 19.5418 4 TAGCGCG 245 1.7425191E-7 19.40409 4 CATGGGG 4915 0.0 19.151424 4 >>END_MODULE