FastQCFastQC Report
Thu 26 May 2016
SRR938637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447436
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.32107070419818945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.24127290764702328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41580.16989208297990221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63850.036.6441571
GTATCAA97750.034.026931
ATCAACG118600.027.9303323
TCAACGC119900.027.7464164
TATCAAC121000.027.3370632
CAACGCA121200.027.2919545
GTACATG98600.027.0158751
TACATGG96750.026.8206732
AACGCAG125150.026.4658326
TCGCGTA1109.4698575E-525.913549
ACATGGG101200.024.7020653
GGACCGA8000.024.3544036
ACGCAGA140850.023.139937
CGCAGAG142900.022.8079748
GAGTACT74800.022.0673612-13
AGGACCG10150.022.0069475
GCAGAGT149950.021.164029
GTACTTT78100.021.0762714-15
GTAAGGT9700.020.5781144
CATGGGG52300.020.264284
Produced by FastQC (version 0.11.5)