##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938637_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2447436 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.984586726680494 31.0 31.0 34.0 28.0 34.0 2 31.12108263505154 31.0 31.0 34.0 27.0 34.0 3 31.182644203975098 31.0 31.0 34.0 27.0 34.0 4 34.84865222216229 37.0 35.0 37.0 32.0 37.0 5 34.85437412867997 37.0 35.0 37.0 32.0 37.0 6 34.85191441165367 37.0 35.0 37.0 32.0 37.0 7 34.76617570387949 37.0 35.0 37.0 32.0 37.0 8 34.723798293397664 37.0 35.0 37.0 31.0 37.0 9 36.18273041664828 39.0 35.0 39.0 31.0 39.0 10-11 36.03502379633216 39.0 35.0 39.0 30.0 39.0 12-13 35.85518375148523 38.0 35.0 39.0 30.0 39.0 14-15 36.80568684942119 39.0 36.0 41.0 30.5 41.0 16-17 36.62721354102824 39.0 36.0 40.5 30.0 41.0 18-19 36.49429464141248 39.0 36.0 40.0 30.0 41.0 20-21 36.474323128367814 39.0 36.0 40.5 29.5 41.0 22-23 36.5736035589899 39.0 36.0 41.0 30.0 41.0 24-25 36.48016863362311 39.0 36.0 41.0 29.5 41.0 26-27 36.279432230301424 39.0 35.0 41.0 29.0 41.0 28-29 36.077402432586595 39.0 35.0 40.0 28.0 41.0 30-31 35.80496609512976 38.0 35.0 40.0 27.0 41.0 32-33 35.49906105818498 38.0 34.0 40.0 27.0 41.0 34-35 35.21830928367483 38.0 34.0 40.0 26.0 41.0 36-37 34.919017085635744 38.0 34.0 40.0 25.0 41.0 38-39 34.58743987585375 38.0 33.0 40.0 24.5 41.0 40-41 34.46080530808568 38.0 33.0 40.0 24.0 41.0 42-43 34.21263027919831 38.0 33.0 40.0 23.5 41.0 44-45 33.88808144523493 38.0 33.0 40.0 22.5 41.0 46-47 33.47274841916193 38.0 32.0 40.0 20.0 41.0 48-49 33.0705054187321 37.0 31.5 40.0 17.5 41.0 50-51 32.280954231285314 36.5 30.5 39.5 15.0 40.5 52-53 32.29281358123359 36.5 31.0 39.0 14.5 40.0 54-55 32.34775945111537 37.0 31.0 39.5 12.0 41.0 56-57 31.994869733059414 36.0 30.5 39.0 8.5 41.0 58-59 31.53099958487168 36.0 30.0 39.0 7.5 41.0 60-61 31.017892807002923 35.0 29.0 39.0 4.0 40.0 62-63 30.396165211265995 35.0 28.0 38.5 2.0 40.0 64-65 29.87025523854352 34.5 28.0 38.0 2.0 40.0 66-67 29.27500106233626 34.0 26.5 38.0 2.0 40.0 68-69 28.61768029889239 34.0 26.0 37.0 2.0 39.5 70-71 27.949685711904216 33.5 25.5 36.5 2.0 39.0 72-73 27.270512691649547 33.0 23.5 36.0 2.0 39.0 74-75 26.536002167165964 32.0 22.0 35.5 2.0 38.5 76-77 25.759952660662016 32.0 19.5 35.0 2.0 37.5 78-79 24.927453261290594 31.0 15.5 35.0 2.0 37.0 80-81 24.10482623447559 31.0 7.5 34.5 2.0 36.0 82-83 23.322204543857325 30.0 3.5 34.0 2.0 36.0 84-85 22.554689887702885 29.5 2.0 34.0 2.0 35.0 86-87 21.789463544705562 29.0 2.0 34.0 2.0 35.0 88-89 20.987731242001836 29.0 2.0 34.0 2.0 35.0 90-91 20.135682812543415 26.5 2.0 34.0 2.0 35.0 92-93 19.249468423280526 25.5 2.0 33.5 2.0 35.0 94-95 18.327823281180795 24.5 2.0 33.0 2.0 35.0 96-97 17.23595080729384 20.5 2.0 32.5 2.0 34.5 98-99 15.991570157503608 2.0 2.0 32.0 2.0 34.0 100-101 13.325936408551645 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7341890889170699 1101 2 0.7199070086043413 1101 3 0.6235054267512297 1101 4 0.5435886166340467 1101 5 0.5245030612839372 1101 6 0.6150704421117226 1101 7 0.604973554003827 1101 8 0.5888112820537259 1101 9 0.6768200040656183 1101 10-11 0.7841526233279055 1101 12-13 0.8470121512942015 1101 14-15 1.0146760861726491 1101 16-17 1.115361589179983 1101 18-19 1.2324527677442632 1101 20-21 1.195179730860616 1101 22-23 1.0984103229750701 1101 24-25 1.208305273586923 1101 26-27 1.1760888722329526 1101 28-29 1.2616867385354027 1101 30-31 1.2663934729796225 1101 32-33 1.3350158481719134 1101 34-35 1.4838138892561474 1101 36-37 1.399117367755501 1101 38-39 1.4953290645605577 1101 40-41 1.67970435943964 1101 42-43 1.6679049696551118 1101 44-45 1.665532834259686 1101 46-47 1.9971267353442954 1101 48-49 1.9699998985956881 1101 50-51 1.9190243821189625 1101 52-53 2.2155526227734725 1101 54-55 2.3774994248425045 1101 56-57 2.532488171491785 1101 58-59 2.5979384850628975 1101 60-61 2.569620258884015 1101 62-63 2.7061464530491257 1101 64-65 2.7726151693174046 1101 66-67 2.8097511253428706 1101 68-69 2.9235073079029377 1101 70-71 3.1495457283574986 1101 72-73 3.3477294216755453 1101 74-75 3.544403086519562 1101 76-77 3.823062166831658 1101 78-79 4.2190387661089375 1101 80-81 4.588591215213754 1101 82-83 4.555203807217946 1101 84-85 4.979623215046242 1101 86-87 5.248095837712562 1101 88-89 5.616614831213532 1101 90-91 5.70219904568118 1101 92-93 5.593608148521795 1101 94-95 5.986717873044302 1101 96-97 5.9972150235085415 1101 98-99 6.3052803017916474 1101 100-101 6.186393400808001 1102 1 0.6434549883539198 1102 2 0.5992349509951538 1102 3 0.585257879828454 1102 4 0.4835925594116972 1102 5 0.45290556680754435 1102 6 0.5757375154548257 1102 7 0.6333425831134321 1102 8 0.595853975640388 1102 9 0.797349600935199 1102 10-11 0.8016163386147213 1102 12-13 0.8695473573229293 1102 14-15 1.07765916942477 1102 16-17 1.0940532937245848 1102 18-19 1.1362763321640443 1102 20-21 1.147847057136623 1102 22-23 0.8789677796603073 1102 24-25 0.8541990194488633 1102 26-27 1.0427761206948958 1102 28-29 1.0557361199656654 1102 30-31 1.177553207548847 1102 32-33 1.1993379879842934 1102 34-35 1.2408848518146343 1102 36-37 1.3521549803664428 1102 38-39 1.3193824259644842 1102 40-41 1.5592474807804848 1102 42-43 1.717024353986659 1102 44-45 1.7800870772091741 1102 46-47 1.7987841897977717 1102 48-49 1.7799913063160915 1102 50-51 1.9838523877429992 1102 52-53 1.9951387055122964 1102 54-55 2.176210582903245 1102 56-57 2.226517653053726 1102 58-59 2.4098479053257975 1102 60-61 2.435624216540077 1102 62-63 2.689950377972689 1102 64-65 2.9173934066726233 1102 66-67 2.957772669275087 1102 68-69 3.156872691803013 1102 70-71 3.263444391532701 1102 72-73 3.36205211942816 1102 74-75 3.4158411960692696 1102 76-77 3.703826805323583 1102 78-79 4.045188494949986 1102 80-81 4.212273360382365 1102 82-83 4.495409724360101 1102 84-85 4.779417149120025 1102 86-87 5.032492853104273 1102 88-89 5.417087034631102 1102 90-91 5.680164126061769 1102 92-93 5.4704447017902105 1102 94-95 5.771720625549452 1102 96-97 5.766226093042771 1102 98-99 5.866707661692857 1102 100-101 5.718800987307336 1103 1 0.5961271182831496 1103 2 0.5366177585823593 1103 3 0.5455453728254938 1103 4 0.549433257764143 1103 5 0.5518374008402205 1103 6 0.5780184799769614 1103 7 0.4874037289693902 1103 8 0.4206384559349132 1103 9 0.6671602637297553 1103 10-11 0.6431439702989792 1103 12-13 0.8026415657310579 1103 14-15 0.9405694143283299 1103 16-17 1.0350922902145356 1103 18-19 1.1212567667478126 1103 20-21 1.0619503779694313 1103 22-23 0.9166555018971394 1103 24-25 1.0553133477934225 1103 26-27 1.0132957975868422 1103 28-29 1.215113513835071 1103 30-31 1.2058019384407714 1103 32-33 1.37511267270812 1103 34-35 1.299891231655252 1103 36-37 1.463120474346752 1103 38-39 1.5496022760725268 1103 40-41 1.6507593915313166 1103 42-43 1.7293074051054234 1103 44-45 1.6360004348575572 1103 46-47 2.003248148843312 1103 48-49 2.1279409393037554 1103 50-51 2.1965376803810273 1103 52-53 2.218127283539573 1103 54-55 2.5582528464889904 1103 56-57 2.528327061031952 1103 58-59 2.798652671341145 1103 60-61 3.047760920060419 1103 62-63 3.1659237895867385 1103 64-65 3.1969548355053874 1103 66-67 3.00235795105138 1103 68-69 3.210358880248414 1103 70-71 3.431915273505645 1103 72-73 3.4857558570086233 1103 74-75 3.839284537825211 1103 76-77 3.9221952153941295 1103 78-79 4.099398918214678 1103 80-81 4.423439439181397 1103 82-83 4.8028007782914806 1103 84-85 4.869384835455964 1103 86-87 5.034985692933688 1103 88-89 5.294227225340318 1103 90-91 5.573985299014904 1103 92-93 5.536476909695267 1103 94-95 5.788243326577319 1103 96-97 5.861670380913072 1103 98-99 5.898543981050754 1103 100-101 5.789731337855567 1104 1 0.5857688474580414 1104 2 0.5191505668919838 1104 3 0.44329337505809363 1104 4 0.4993668924075436 1104 5 0.460521733170701 1104 6 0.5158685146911495 1104 7 0.4505903551588659 1104 8 0.5804098187730347 1104 9 0.527995854619661 1104 10-11 0.6540123184482383 1104 12-13 0.7015915466722831 1104 14-15 0.7712001745009402 1104 16-17 0.9245482814747277 1104 18-19 1.2015414060606133 1104 20-21 1.1900772923547223 1104 22-23 1.0933712165329155 1104 24-25 1.1322350502860346 1104 26-27 1.0056915188931796 1104 28-29 0.8866107843470701 1104 30-31 1.041902565338141 1104 32-33 1.1070062464995871 1104 34-35 1.2595182923846622 1104 36-37 1.3035284681390422 1104 38-39 1.4898665633426376 1104 40-41 1.5863404968037855 1104 42-43 1.7312410193287064 1104 44-45 1.7772439514727196 1104 46-47 1.7741642119822885 1104 48-49 1.868597292680569 1104 50-51 2.0202157335277633 1104 52-53 2.1104391122284696 1104 54-55 2.2494614787500424 1104 56-57 2.374963613709035 1104 58-59 2.3796589913651047 1104 60-61 2.694786560911524 1104 62-63 2.782127657155524 1104 64-65 2.937592980951024 1104 66-67 3.0992318024202063 1104 68-69 3.2514106179997526 1104 70-71 3.327922035965315 1104 72-73 3.428971615887967 1104 74-75 3.3826622962417012 1104 76-77 3.5916233332045486 1104 78-79 3.9254206894549952 1104 80-81 4.12773574518355 1104 82-83 4.4809232683196605 1104 84-85 4.737508278422599 1104 86-87 4.871514971967677 1104 88-89 5.043743098572275 1104 90-91 5.4092519345976235 1104 92-93 5.341689142702332 1104 94-95 5.610520985615938 1104 96-97 5.873934954064712 1104 98-99 5.913530299577243 1104 100-101 5.6862729370224265 1105 1 0.5661844867676571 1105 2 0.45607833407567 1105 3 0.48078998427373776 1105 4 0.3696247539663702 1105 5 0.3512643857964832 1105 6 0.37858446210666585 1105 7 0.338707832172382 1105 8 0.3379357405602903 1105 9 0.5042324281257891 1105 10-11 0.6367648660912835 1105 12-13 0.6460328871151333 1105 14-15 0.7719589004567453 1105 16-17 0.8276483693314702 1105 18-19 0.9496646322249092 1105 20-21 0.8298209288140512 1105 22-23 0.8524437408554633 1105 24-25 0.8450082867745223 1105 26-27 0.9477425081924693 1105 28-29 1.0848783465856755 1105 30-31 1.0759581863476413 1105 32-33 0.9592886998484715 1105 34-35 0.9942933669819141 1105 36-37 1.0044786974977669 1105 38-39 1.1133558929997562 1105 40-41 1.1376330435561215 1105 42-43 1.2424279760440768 1105 44-45 1.3099562145732904 1105 46-47 1.303828615426923 1105 48-49 1.2935793771930761 1105 50-51 1.3008467635569545 1105 52-53 1.5047855107093895 1105 54-55 1.7271956655488623 1105 56-57 1.7861942963599589 1105 58-59 1.8172936611735508 1105 60-61 2.1611001811355806 1105 62-63 2.306923501572168 1105 64-65 2.4300069360023784 1105 66-67 2.6354255407042864 1105 68-69 2.6710192708765526 1105 70-71 2.9387729507685734 1105 72-73 3.1424116728936298 1105 74-75 3.2107764533230743 1105 76-77 3.254954277806217 1105 78-79 3.7204525324626907 1105 80-81 4.077397881357445 1105 82-83 4.250181101537603 1105 84-85 4.514639209258693 1105 86-87 4.549952822433077 1105 88-89 4.905105971204293 1105 90-91 5.13607496197087 1105 92-93 5.224082570856165 1105 94-95 5.502201316637464 1105 96-97 5.931003777877532 1105 98-99 5.954986130309287 1105 100-101 5.486458772248525 1106 1 0.29994866572680934 1106 2 0.2773820680710912 1106 3 0.3116404784074227 1106 4 0.2915418952934701 1106 5 0.29165433760019255 1106 6 0.13329211996168766 1106 7 0.340898572642665 1106 8 0.2123405893992043 1106 9 0.3095267175956309 1106 10-11 0.19340280971623258 1106 12-13 0.12405976019823584 1106 14-15 0.5886340866696784 1106 16-17 0.3815977875681611 1106 18-19 0.6247096641123591 1106 20-21 0.5410143955391433 1106 22-23 0.4077270766229546 1106 24-25 0.5755532943204074 1106 26-27 0.6172851302654081 1106 28-29 0.4755762191332664 1106 30-31 0.7182333920338309 1106 32-33 0.8232680582049241 1106 34-35 0.6448276642410562 1106 36-37 0.6958617849813393 1106 38-39 0.8802964022252056 1106 40-41 0.7527029038355693 1106 42-43 0.4721697120841952 1106 44-45 0.9792030866827304 1106 46-47 1.3328121118487104 1106 48-49 1.3869621238947971 1106 50-51 0.704388460650577 1106 52-53 1.1181709349827926 1106 54-55 0.9348413497901404 1106 56-57 1.1440371472435586 1106 58-59 0.5648838466562509 1106 60-61 1.7995062957356538 1106 62-63 1.9998048121218055 1106 64-65 2.0577404932780716 1106 66-67 2.258954093355083 1106 68-69 1.9176138865233092 1106 70-71 2.349838624299558 1106 72-73 2.7340698200140245 1106 74-75 3.112620326552225 1106 76-77 3.30640260067268 1106 78-79 3.582887418787603 1106 80-81 4.121160356407348 1106 82-83 4.370227593918695 1106 84-85 4.512751521220135 1106 86-87 4.876165032159967 1106 88-89 4.7300889321117445 1106 90-91 5.001950527912161 1106 92-93 5.521902784088237 1106 94-95 5.3644000898228015 1106 96-97 5.423073297375122 1106 98-99 5.348624463399201 1106 100-101 4.589882412772354 1107 1 0.4055613966302971 1107 2 0.36750033243006897 1107 3 0.4011818202980706 1107 4 0.4170739583213816 1107 5 0.4398938221359501 1107 6 0.34236480333157004 1107 7 0.354019365586673 1107 8 0.32613435306669203 1107 9 0.2480537772531477 1107 10-11 0.42348189524823 1107 12-13 0.5049572338284847 1107 14-15 0.6937153874826905 1107 16-17 0.7773668381936645 1107 18-19 0.7947008346351936 1107 20-21 0.7329929421249162 1107 22-23 0.6998481923650601 1107 24-25 0.8279501492543133 1107 26-27 0.8409885579380543 1107 28-29 0.8151886873677157 1107 30-31 0.7976846827514876 1107 32-33 0.965906837648383 1107 34-35 0.9040842950518169 1107 36-37 0.863975663083913 1107 38-39 1.0836719366617515 1107 40-41 1.119531760718921 1107 42-43 1.2849820271477839 1107 44-45 1.2966892490881392 1107 46-47 1.039638816744798 1107 48-49 1.0897760845957123 1107 50-51 1.1755095827220572 1107 52-53 1.4158023248817457 1107 54-55 1.550700388294885 1107 56-57 1.7731907200171193 1107 58-59 1.9128293317553187 1107 60-61 1.9065034190350332 1107 62-63 2.111826113631036 1107 64-65 2.040937507712208 1107 66-67 2.1932877842980645 1107 68-69 2.5187563385862113 1107 70-71 2.5402894972785077 1107 72-73 2.8582573657041976 1107 74-75 2.9347477400217663 1107 76-77 3.2891221429323316 1107 78-79 3.446690597399378 1107 80-81 3.8822898490763507 1107 82-83 4.1099136411294985 1107 84-85 4.358276574466 1107 86-87 4.633471266277191 1107 88-89 4.955170264571624 1107 90-91 5.362073412714384 1107 92-93 5.371789725639633 1107 94-95 5.291375012841787 1107 96-97 5.5579323263858065 1107 98-99 5.589474957248637 1107 100-101 5.076545550812845 1108 1 0.36312656548724576 1108 2 0.4415644384330797 1108 3 0.3981117213101051 1108 4 0.37471017477209045 1108 5 0.2940924188255494 1108 6 0.22775479892722217 1108 7 0.220494143540364 1108 8 0.43056347812461127 1108 9 0.47799765868555255 1108 10-11 0.46054954730890074 1108 12-13 0.5387618144113517 1108 14-15 0.5177679783566234 1108 16-17 0.6189523043847913 1108 18-19 0.7477313757679909 1108 20-21 0.6954958434858654 1108 22-23 0.7549206895103566 1108 24-25 0.7485839660424318 1108 26-27 0.8244112125471048 1108 28-29 0.9256710072041159 1108 30-31 1.104769241731077 1108 32-33 1.1798221109844391 1108 34-35 1.2003003784809536 1108 36-37 1.2764259862149956 1108 38-39 1.2605281378372197 1108 40-41 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0.6370284627265548 2311 56-57 0.5593227244979531 2311 58-59 0.7563994130146625 2311 60-61 0.7857736291993653 2311 62-63 0.893482114015093 2311 64-65 1.0732796117276777 2311 66-67 0.939805097741413 2311 68-69 0.9853589763453421 2311 70-71 1.3194928397420576 2311 72-73 1.253492146539699 2311 74-75 1.4051288710534813 2311 76-77 1.5753211042907047 2311 78-79 1.834340449640937 2311 80-81 1.8838274048991401 2311 82-83 1.9765284069268247 2311 84-85 2.3764047960690355 2311 86-87 2.5622649679015908 2311 88-89 2.5400580601535445 2311 90-91 2.6371683079250126 2311 92-93 2.8968906774379626 2311 94-95 3.16194826032169 2311 96-97 2.9134103278931924 2311 98-99 2.9819477854640404 2311 100-101 2.887615908148547 2312 1 -0.3010337064617019 2312 2 -0.30344476817190724 2312 3 -0.21928900731414203 2312 4 -0.15808253194669675 2312 5 -0.23046078750481058 2312 6 -0.01693319336940391 2312 7 -0.22379789118135562 2312 8 -0.27037229834138543 2312 9 -0.2803024930799154 2312 10-11 -0.2642667893594677 2312 12-13 -0.17876727012353655 2312 14-15 0.08722527185418727 2312 16-17 0.027955550567654086 2312 18-19 0.05497776574898694 2312 20-21 0.009999772916224003 2312 22-23 -0.1004840477299922 2312 24-25 -0.08179569571183265 2312 26-27 0.13151163536148402 2312 28-29 -0.06815784138898806 2312 30-31 0.14181692020405734 2312 32-33 0.20612028844301022 2312 34-35 0.07320812232762108 2312 36-37 0.22734560821195515 2312 38-39 0.39428288645782317 2312 40-41 0.31000117562470564 2312 42-43 0.31017647754908495 2312 44-45 0.2610555079671073 2312 46-47 0.26053884286461937 2312 48-49 0.32698321479947623 2312 50-51 0.4450183979085409 2312 52-53 0.5676281209011691 2312 54-55 0.8094802571266158 2312 56-57 0.8253617652267806 2312 58-59 1.0506713667843428 2312 60-61 1.13213245452242 2312 62-63 1.2091747990518051 2312 64-65 1.2995786467397998 2312 66-67 1.5217206863324648 2312 68-69 1.6856315234322388 2312 70-71 1.7930023509759891 2312 72-73 2.0048774909777194 2312 74-75 2.026116306998791 2312 76-77 2.1335023662619044 2312 78-79 2.5228827336046074 2312 80-81 2.5666688731299665 2312 82-83 2.730289441366711 2312 84-85 2.860578066344967 2312 86-87 3.1344499974228945 2312 88-89 3.287590759181491 2312 90-91 3.3893843819224614 2312 92-93 3.375383996352806 2312 94-95 3.313862961642865 2312 96-97 3.422413126282546 2312 98-99 3.385657499246161 2312 100-101 3.0458827069230026 2313 1 -0.49050891393575924 2313 2 -0.49133420682786877 2313 3 -0.5116727570388555 2313 4 -0.5950461356466121 2313 5 -0.3954778412804103 2313 6 -0.4539228333679475 2313 7 -0.5535169739038537 2313 8 -0.34541826103928486 2313 9 -0.4921690612210057 2313 10-11 -0.4798309434466006 2313 12-13 -0.4811921845310039 2313 14-15 -0.5210937139442322 2313 16-17 -0.5130721179623592 2313 18-19 -0.4947348064603858 2313 20-21 -0.42836274240887207 2313 22-23 -0.5436803241652797 2313 24-25 -0.44254695368940844 2313 26-27 -0.27368344052712956 2313 28-29 -0.20779230901788281 2313 30-31 -0.20044612034941167 2313 32-33 -0.20495050396527148 2313 34-35 -0.08330585031816184 2313 36-37 0.042948845516427525 2313 38-39 0.06923041468527202 2313 40-41 0.12319396952524642 2313 42-43 0.21873606176234972 2313 44-45 0.16618398271614154 2313 46-47 0.2305428691408622 2313 48-49 0.09912424580526391 2313 50-51 0.26411790734728413 2313 52-53 0.24031984119996963 2313 54-55 0.3190014869950559 2313 56-57 0.3374877757336421 2313 58-59 0.3799981788639748 2313 60-61 0.5058432441803404 2313 62-63 0.7027846139020753 2313 64-65 0.6489274443878443 2313 66-67 1.0396209293331218 2313 68-69 1.2311186632988047 2313 70-71 1.2462717980412457 2313 72-73 1.3078129182240303 2313 74-75 1.4274995824600971 2313 76-77 1.5007867932612307 2313 78-79 1.636633668070381 2313 80-81 1.759857489714971 2313 82-83 1.9939470982753278 2313 84-85 2.111238144053967 2313 86-87 2.129308188061252 2313 88-89 2.1952991290459565 2313 90-91 2.3966076567048127 2313 92-93 2.542562925996254 2313 94-95 2.6835674697920133 2313 96-97 2.736821067324861 2313 98-99 2.6976837662753006 2313 100-101 2.4935578021101286 2314 1 -0.530414297509477 2314 2 -0.4597949118681477 2314 3 -0.5722863139618646 2314 4 -0.4203260554586521 2314 5 -0.5201084509807927 2314 6 -0.5101004977768966 2314 7 -0.4141842656289896 2314 8 -0.2749151084225261 2314 9 -0.2808770805421972 2314 10-11 -0.40791725227410325 2314 12-13 -0.41158191354073637 2314 14-15 -0.31498355351579477 2314 16-17 -0.36790100091442923 2314 18-19 -0.2950857308915147 2314 20-21 -0.31723314760194654 2314 22-23 -0.42164084337999697 2314 24-25 -0.368389426120423 2314 26-27 -0.4555135364504608 2314 28-29 -0.3421365343936671 2314 30-31 -0.4800351220431409 2314 32-33 -0.3843119203936496 2314 34-35 -0.316512630705013 2314 36-37 -0.33785222311184526 2314 38-39 -0.09253701105598111 2314 40-41 0.016936388454830364 2314 42-43 0.14303825322043906 2314 44-45 0.13226016944719987 2314 46-47 0.08653060932113021 2314 48-49 0.24021077400610835 2314 50-51 0.32277861845533096 2314 52-53 0.41780440484245673 2314 54-55 0.39369957150397283 2314 56-57 0.45145077281702584 2314 58-59 0.8104144543829008 2314 60-61 0.8276316416638494 2314 62-63 0.8175695317508413 2314 64-65 1.0938141928955503 2314 66-67 1.2595484748662216 2314 68-69 1.3894251972990475 2314 70-71 1.4063448417823068 2314 72-73 1.385556850012918 2314 74-75 1.6726986754446393 2314 76-77 1.8448455240580408 2314 78-79 1.9969487080389214 2314 80-81 2.123417038202607 2314 82-83 2.4158879631547627 2314 84-85 2.6706694332283014 2314 86-87 2.6626944345190395 2314 88-89 2.6667745402688894 2314 90-91 2.8041915276292677 2314 92-93 2.8267288667666115 2314 94-95 2.995156027488079 2314 96-97 2.994579576356216 2314 98-99 3.0353257536344955 2314 100-101 2.8214547409767263 2315 1 -0.6030082715435796 2315 2 -0.5675166051512015 2315 3 -0.6368601062586734 2315 4 -0.513626184699902 2315 5 -0.3248208163925739 2315 6 -0.3408789892465762 2315 7 -0.32276650115417027 2315 8 -0.512466286642379 2315 9 -0.4562344071730351 2315 10-11 -0.5364906613589113 2315 12-13 -0.5307612030736877 2315 14-15 -0.6194216453598855 2315 16-17 -0.4492069799925389 2315 18-19 -0.6303106380725438 2315 20-21 -0.4011397997853763 2315 22-23 -0.40172402779748495 2315 24-25 -0.4748672509382814 2315 26-27 -0.4940995155742627 2315 28-29 -0.63270325126053 2315 30-31 -0.4602782874770739 2315 32-33 -0.48399008798921983 2315 34-35 -0.5188459263453566 2315 36-37 -0.20022785768180285 2315 38-39 -0.27507585053469796 2315 40-41 -0.2729482852924008 2315 42-43 -0.035422890738779245 2315 44-45 -0.15146748389613407 2315 46-47 -0.03760516480423348 2315 48-49 0.01973680186807769 2315 50-51 0.11701126354904545 2315 52-53 0.24167865533746635 2315 54-55 0.3641579810085389 2315 56-57 0.5172101807858951 2315 58-59 0.5617142198297458 2315 60-61 0.8454592093984239 2315 62-63 0.8726231328871137 2315 64-65 0.980434054585146 2315 66-67 1.0756230618567741 2315 68-69 1.416775097840972 2315 70-71 1.3656476272366973 2315 72-73 1.5024596403788202 2315 74-75 1.6766069667017511 2315 76-77 1.7619375931686996 2315 78-79 1.7639453882008205 2315 80-81 1.9987273628248623 2315 82-83 2.083172423536869 2315 84-85 2.1567909194283885 2315 86-87 2.387158366045739 2315 88-89 2.654684729881726 2315 90-91 2.769456443628169 2315 92-93 2.613157422285809 2315 94-95 2.627294279070469 2315 96-97 2.6329398830171975 2315 98-99 2.7912938094018394 2315 100-101 2.6464622081852625 2316 1 -0.953694180092814 2316 2 -0.8128013359515656 2316 3 -0.8424548114511659 2316 4 -0.7568228680994835 2316 5 -0.7393300719255578 2316 6 -0.8139666870871878 2316 7 -0.6127279353693353 2316 8 -0.6774003997337275 2316 9 -0.768953154548889 2316 10-11 -0.8004826444586399 2316 12-13 -0.7777595598254479 2316 14-15 -1.2461970677500958 2316 16-17 -1.036964307424057 2316 18-19 -0.8058368598168713 2316 20-21 -0.6979969729868003 2316 22-23 -0.8438862414401882 2316 24-25 -1.0832061160950346 2316 26-27 -0.7973704493385441 2316 28-29 -0.7230620003368742 2316 30-31 -0.646728076621848 2316 32-33 -0.5588667917945926 2316 34-35 -0.6352951281329169 2316 36-37 -0.4266520374509568 2316 38-39 -0.5930586555394584 2316 40-41 -0.4920178814144691 2316 42-43 -0.39111155055334024 2316 44-45 -0.25971065939862825 2316 46-47 -0.03352731338398485 2316 48-49 -0.041631061460059016 2316 50-51 -8.837983991156761E-4 2316 52-53 0.14291236086899772 2316 54-55 -0.14593487400870941 2316 56-57 0.3164914418223148 2316 58-59 0.46832982998198247 2316 60-61 0.4921756346853101 2316 62-63 0.6800728653992287 2316 64-65 0.7543444130578187 2316 66-67 0.928956584466345 2316 68-69 1.1511892135221018 2316 70-71 0.9930306113328413 2316 72-73 1.1848414174633888 2316 74-75 1.5704502612980527 2316 76-77 1.6509837866357522 2316 78-79 1.8609820583690961 2316 80-81 2.2830419056844384 2316 82-83 2.3361914922166527 2316 84-85 2.2188674420092696 2316 86-87 2.7829690819951303 2316 88-89 2.758648309528045 2316 90-91 2.93047359010259 2316 92-93 3.098583151866844 2316 94-95 3.1226941153615435 2316 96-97 2.9519488356330896 2316 98-99 3.011069586159227 2316 100-101 3.0186878278659037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7829.0 3 2962.0 4 3190.0 5 3826.0 6 4503.0 7 5541.0 8 7339.0 9 9298.0 10 11626.0 11 14729.0 12 17910.0 13 21154.0 14 24235.0 15 28108.0 16 32282.0 17 36554.0 18 36299.0 19 35779.0 20 38058.0 21 41833.0 22 46234.0 23 51601.0 24 57576.0 25 63755.0 26 70765.0 27 78546.0 28 86699.0 29 96436.0 30 106824.0 31 119893.0 32 135290.0 33 154177.0 34 177919.0 35 207573.0 36 236301.0 37 237487.0 38 128503.0 39 8802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05941527409129 18.299325694593588 14.311406794860654 23.329852236454464 2 15.48555497105926 19.833471691372875 40.14206121188563 24.538912125682234 3 19.470008314332453 24.014727078153907 29.253247045223592 27.262017562290048 4 12.953402187269297 16.257769776702975 36.6485334405957 34.14029459543203 5 14.050330787930227 37.25180684372468 33.75091681118963 14.94694555715546 6 32.61061892633309 35.52749171099055 17.321106618533857 14.540782744142502 7 29.246826114998004 32.35797319312736 20.17091690556124 18.224283786313407 8 26.588247507966173 34.80247658379234 19.5380847512217 19.071191157019793 9 26.649327058046403 16.910369511065547 19.908870996091434 36.53143243479661 10-11 25.21450340354597 26.47092062398241 27.030994962682485 21.283581009789142 12-13 26.177112117000323 23.769906010055212 27.348660370969164 22.7043215019753 14-15 22.84302724428304 25.213575569223213 25.89505559146511 26.048341595028635 16-17 22.495012011224098 28.43265990376308 26.418276677464096 22.65405140754872 18-19 22.491284004439276 26.93642641423299 28.77403476262514 21.798254818702592 20-21 23.09509673582378 26.151014773250985 28.84314520949976 21.910743281425475 22-23 22.70650680963254 26.080760813432434 28.687555548016885 22.525176828918138 24-25 22.603456536562792 26.167325116877638 28.899355231163447 22.329863115396126 26-27 22.28180802932717 26.52579453647338 28.737023429362818 22.455374004836628 28-29 22.227249791079988 26.889298043069914 28.73067579313475 22.152776372715344 30-31 22.314287571157458 26.436954503781102 28.652906118141303 22.59585180692014 32-33 22.14489771686642 26.492842868035453 28.559882008431646 22.802377406666473 34-35 22.87487212059309 26.548929736728844 28.347129799590544 22.229068343087523 36-37 22.831775939672923 26.18350508090102 28.4330184486009 22.551700530825155 38-39 22.381010081773546 26.542880793382594 28.393579655900908 22.682529468942953 40-41 22.675809753288686 26.36620133889182 28.452615890237187 22.505373017582304 42-43 22.527190609817126 26.345002132256283 28.219916438439295 22.90789081948729 44-45 22.418080181104198 26.658317022241658 28.219707340195573 22.70389545645858 46-47 22.72052985112138 26.912678119203427 27.693789238941786 22.673002790733406 48-49 22.65119756410877 26.654922015968886 27.85419224571084 22.8396881742115 50-51 22.680930727081755 26.973531112940364 27.674230402495514 22.67130775748237 52-53 22.917423024172862 26.8978537868407 27.48598921142209 22.698733977564352 54-55 22.460375088721637 26.953152933013687 27.75374183628146 22.83273014198322 56-57 22.59054550714535 27.03286469960771 27.64588934338227 22.730700449864678 58-59 22.67898935436649 26.585092026858746 27.680812831558722 23.055105787216046 60-61 22.51621857239787 26.77808735156684 27.992827931024404 22.71286614501088 62-63 22.395650097106262 26.935536397175845 27.910685121146855 22.758128384571037 64-65 22.16965027989826 26.839034442431508 27.85222140065793 23.1390938770123 66-67 22.26919564511219 26.879787036261447 27.729905428900064 23.121111889726297 68-69 22.60096823261283 26.658535065486973 27.76065306155806 22.97984364034214 70-71 22.44509742103307 26.709314696734012 27.59442920749161 23.25115867474131 72-73 22.592807134224397 26.62788571265111 27.641911627236983 23.137395525887513 74-75 22.40874959417135 26.984351410730877 27.48248117659967 23.124417818498106 76-77 22.570725254895326 26.67585683282111 27.442371446983284 23.31104646530028 78-79 22.65982044187325 26.81603760574727 27.458872289318105 23.06526966306138 80-81 22.749994841159822 26.94557229516902 27.252119772537032 23.05231309113413 82-83 22.755178243968718 26.842647787465967 27.312768489715022 23.08940547885029 84-85 22.797909147835753 26.649375696550624 27.364380124850474 23.18833503076315 86-87 22.879151065917544 26.72468337297747 27.209013979537865 23.18715158156712 88-89 22.855630762943473 26.671972618834765 27.16127812827259 23.31111848994917 90-91 22.87579882846666 26.594938995581003 27.189022964734654 23.340239211217686 92-93 23.063632557629198 26.28799489679266 27.245193624995583 23.40317892058256 94-95 22.814599362288373 26.34087411121292 27.318754459771938 23.525772066726773 96-97 23.017961448538454 26.168499482828306 27.270436863982006 23.543102204651234 98-99 23.024444743666 26.225429750146144 26.893815328856864 23.856310177330997 100-101 23.555045726148666 25.707570659922084 27.19990559657301 23.537478017356243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 646.0 1 439.5 2 311.0 3 415.5 4 360.0 5 345.0 6 626.0 7 1353.0 8 2200.5 9 2523.5 10 2249.5 11 1953.0 12 1955.5 13 2043.5 14 2219.5 15 2434.5 16 2582.5 17 2814.0 18 3155.0 19 3408.5 20 3730.0 21 4320.0 22 5136.5 23 6187.0 24 7580.0 25 9496.0 26 11898.5 27 14888.0 28 18107.5 29 21927.5 30 26171.5 31 30984.5 32 36599.0 33 39716.5 34 42130.5 35 46023.0 36 50337.5 37 55073.0 38 59261.0 39 62900.0 40 66850.5 41 72601.5 42 78248.5 43 83549.5 44 88220.0 45 91763.0 46 93572.0 47 95677.0 48 99614.5 49 100035.0 50 97032.5 51 92922.5 52 88817.0 53 86145.5 54 84122.0 55 78891.0 56 71317.0 57 64932.5 58 59172.0 59 52297.0 60 43998.5 61 36531.0 62 30025.5 63 23428.0 64 18280.5 65 14490.5 66 11128.0 67 8588.0 68 6592.5 69 4945.5 70 3850.5 71 3130.5 72 2559.5 73 2068.0 74 1661.0 75 1359.5 76 1000.0 77 670.0 78 452.0 79 273.5 80 159.5 81 102.5 82 60.5 83 35.0 84 24.0 85 17.5 86 13.5 87 10.0 88 4.0 89 4.0 90 6.0 91 3.5 92 1.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3102021871051991 2 0.03616029183194167 3 0.043228913851066995 4 0.044863277323697125 5 0.059572548577368316 6 0.05826505779926421 7 0.03064431511181498 8 0.03432163292523278 9 0.041431114031173846 10-11 0.032544262648747505 12-13 0.03450549881590367 14-15 0.028703508488066694 16-17 0.02279937044319034 18-19 0.026211104192305747 20-21 0.038959139278820776 22-23 0.03233996721466874 24-25 0.05262650381869026 26-27 0.04510843184459165 28-29 0.037815084847979685 30-31 0.0377946553045718 32-33 0.03352488073232558 34-35 0.032482974018523875 36-37 0.03160450365198518 38-39 0.02024567751720576 40-41 0.01989837527927186 42-43 0.020327395690837267 44-45 0.009234153620360246 46-47 0.0167113665076431 48-49 0.014893137144342079 50-51 0.006803037954822925 52-53 0.005127815395377039 54-55 0.007150340192756828 56-57 0.005904138044876352 58-59 0.007701937864769497 60-61 0.006721319781191418 62-63 0.004780513157443137 64-65 0.008478260514268811 66-67 0.0029009951639184847 68-69 0.007906233298848264 70-71 0.014361969015737287 72-73 0.01464798262344756 74-75 0.011542692025450309 76-77 0.010419067138017091 78-79 0.006149292565770872 80-81 0.007375065170243471 82-83 0.01219643741450236 84-85 0.0044332109195092334 86-87 0.0015322157555907488 88-89 0.02036825477765302 90-91 0.029745415201868405 92-93 0.014198532668474272 94-95 0.022962806790453358 96-97 0.019326348063851313 98-99 0.014116814494842766 100-101 0.10662178704570824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2447436.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.92082825338913 #Duplication Level Percentage of deduplicated Percentage of total 1 75.54402632245623 42.24484521547582 2 13.590528789608545 15.199872526328798 3 4.782012616972614 8.022423187777964 4 2.165801172538275 4.844535816020066 5 1.137864957833992 3.181517544129226 6 0.6878277306161633 2.3078337835022924 7 0.4393924508445645 1.7199832845660235 8 0.29618011545257206 1.3250109894633797 9 0.21312686992300106 1.0726407980231893 >10 1.0271425511673309 10.113958700524138 >50 0.0688964237821804 2.6650560468290565 >100 0.0427073809590173 4.446609172475599 >500 0.0028723294421399517 1.093307501939234 >1k 0.0014729894575068582 1.1955708383101091 >5k 1.4729894575068583E-4 0.5668345946350901 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7858 0.32107070419818945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5905 0.24127290764702328 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4158 0.16989208297990221 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 4.08590868157533E-5 12-13 0.0 0.0 0.0 0.0 1.634363472630132E-4 14-15 0.0 0.0 0.0 0.0 1.634363472630132E-4 16-17 0.0 0.0 0.0 1.0214771703938326E-4 1.634363472630132E-4 18-19 0.0 4.08590868157533E-5 0.0 1.225772604472599E-4 1.634363472630132E-4 20-21 0.0 4.08590868157533E-5 0.0 1.225772604472599E-4 2.042954340787665E-4 22-23 0.0 4.08590868157533E-5 0.0 3.4730223793390307E-4 2.042954340787665E-4 24-25 0.0 4.08590868157533E-5 0.0 7.150340192756827E-4 2.042954340787665E-4 26-27 0.0 4.08590868157533E-5 0.0 0.0015935043858143786 2.042954340787665E-4 28-29 0.0 4.08590868157533E-5 0.0 0.004106338224983207 2.042954340787665E-4 30-31 0.0 4.08590868157533E-5 0.0 0.011951282893607841 2.042954340787665E-4 32-33 0.0 4.08590868157533E-5 0.0 0.030726033285446486 2.042954340787665E-4 34-35 0.0 8.17181736315066E-5 0.0 0.0593273940564738 2.042954340787665E-4 36-37 0.0 8.17181736315066E-5 0.0 0.10987008444756063 2.042954340787665E-4 38-39 0.0 8.17181736315066E-5 0.0 0.18729805396341315 2.451545208945198E-4 40-41 2.042954340787665E-5 8.17181736315066E-5 0.0 0.2673818641222896 2.451545208945198E-4 42-43 4.08590868157533E-5 8.17181736315066E-5 0.0 0.3464646266541801 2.6558406430239644E-4 44-45 4.08590868157533E-5 8.17181736315066E-5 0.0 0.450675727577759 2.860136077102731E-4 46-47 4.08590868157533E-5 8.17181736315066E-5 0.0 0.5734777130025055 2.860136077102731E-4 48-49 4.08590868157533E-5 8.17181736315066E-5 0.0 0.7069847791729794 3.268726945260264E-4 50-51 4.08590868157533E-5 8.17181736315066E-5 0.0 0.8528517191052187 3.268726945260264E-4 52-53 4.08590868157533E-5 8.17181736315066E-5 0.0 0.9928962391662132 3.6773178134177974E-4 54-55 4.08590868157533E-5 1.634363472630132E-4 0.0 1.1479156145451812 3.6773178134177974E-4 56-57 4.08590868157533E-5 2.042954340787665E-4 0.0 1.3277364556213116 3.6773178134177974E-4 58-59 4.08590868157533E-5 2.451545208945198E-4 0.0 1.5224095747549682 3.6773178134177974E-4 60-61 4.08590868157533E-5 2.860136077102731E-4 0.0 1.7394939030070653 3.6773178134177974E-4 62-63 4.08590868157533E-5 2.860136077102731E-4 0.0 1.9588050514906212 3.6773178134177974E-4 64-65 4.08590868157533E-5 3.268726945260264E-4 0.0 2.187064339986827 3.6773178134177974E-4 66-67 4.08590868157533E-5 3.268726945260264E-4 0.0 2.4531387133310125 3.6773178134177974E-4 68-69 4.08590868157533E-5 3.268726945260264E-4 0.0 2.7427274911376642 4.290204115654097E-4 70-71 4.08590868157533E-5 3.268726945260264E-4 0.0 3.0459427743973695 5.107385851969163E-4 72-73 4.08590868157533E-5 3.268726945260264E-4 0.0 3.3570030023256994 6.128863022362996E-4 74-75 4.08590868157533E-5 3.268726945260264E-4 0.0 3.671863125327894 6.946044758678061E-4 76-77 4.08590868157533E-5 3.268726945260264E-4 0.0 4.009073168818306 6.946044758678061E-4 78-79 4.08590868157533E-5 3.268726945260264E-4 0.0 4.3882046353816815 7.150340192756829E-4 80-81 4.08590868157533E-5 3.268726945260264E-4 0.0 4.793138615269204 7.558931060914361E-4 82-83 4.08590868157533E-5 3.268726945260264E-4 0.0 5.203752008224117 7.763226494993127E-4 84-85 4.08590868157533E-5 3.268726945260264E-4 0.0 5.625683368226993 7.763226494993127E-4 86-87 4.08590868157533E-5 3.268726945260264E-4 0.0 6.059034842994873 7.967521929071893E-4 88-89 4.08590868157533E-5 3.268726945260264E-4 0.0 6.518229690173716 8.17181736315066E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6385 0.0 36.644157 1 GTATCAA 9775 0.0 34.02693 1 ATCAACG 11860 0.0 27.930332 3 TCAACGC 11990 0.0 27.746416 4 TATCAAC 12100 0.0 27.337063 2 CAACGCA 12120 0.0 27.291954 5 GTACATG 9860 0.0 27.015875 1 TACATGG 9675 0.0 26.820673 2 AACGCAG 12515 0.0 26.465832 6 TCGCGTA 110 9.4698575E-5 25.91354 9 ACATGGG 10120 0.0 24.702065 3 GGACCGA 800 0.0 24.354403 6 ACGCAGA 14085 0.0 23.13993 7 CGCAGAG 14290 0.0 22.807974 8 GAGTACT 7480 0.0 22.06736 12-13 AGGACCG 1015 0.0 22.006947 5 GCAGAGT 14995 0.0 21.16402 9 GTACTTT 7810 0.0 21.07627 14-15 GTAAGGT 970 0.0 20.578114 4 CATGGGG 5230 0.0 20.26428 4 >>END_MODULE