FastQCFastQC Report
Thu 26 May 2016
SRR938637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447436
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.3227050676708196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64890.2651346143474232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44670.18251754080597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65500.034.709071
GTATCAA100450.032.954571
ATCAACG124750.025.9893783
TATCAAC126300.025.7954772
GTACATG99300.025.385341
TCAACGC127750.025.3790594
CAACGCA128850.025.1623955
TACATGG97400.024.8550822
AACGCAG132600.024.499956
ACATGGG100650.023.0965523
ACGCAGA149300.021.6958757
CGCAGAG150050.021.5557798
AGTACTT82550.020.3675512-13
AGAGTAC131950.020.22923910-11
GAGTACT80600.020.03532812-13
GCAGAGT161300.019.551789
GTACTTT82750.019.2564914-15
CATGGGA53700.019.0333944
CATGGGG49900.018.7679884
TAAGGTG11350.018.42935
Produced by FastQC (version 0.11.5)