##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938637_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2447436 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804102742625343 31.0 31.0 34.0 30.0 34.0 2 32.04403996672436 33.0 31.0 34.0 30.0 34.0 3 32.110857648575895 33.0 31.0 34.0 30.0 34.0 4 35.775537337850714 37.0 35.0 37.0 35.0 37.0 5 35.647978537538876 37.0 35.0 37.0 33.0 37.0 6 35.6854761472823 37.0 35.0 37.0 33.0 37.0 7 35.61810441621354 37.0 35.0 37.0 33.0 37.0 8 35.609345045181975 37.0 35.0 37.0 33.0 37.0 9 37.18144744132226 39.0 37.0 39.0 33.0 39.0 10-11 37.115977292153914 39.0 37.0 39.0 33.0 39.0 12-13 37.005124342372994 39.0 37.0 39.0 33.0 39.0 14-15 38.055252925919206 40.0 37.0 41.0 33.0 41.0 16-17 37.98551218499687 40.0 37.0 41.0 33.0 41.0 18-19 37.993190833182155 40.0 37.0 41.0 33.0 41.0 20-21 37.98916539594907 40.0 37.0 41.0 33.0 41.0 22-23 38.019784582722494 40.0 37.0 41.0 33.0 41.0 24-25 37.944420609977136 40.0 37.0 41.0 33.0 41.0 26-27 37.77120627464824 40.0 37.0 41.0 32.0 41.0 28-29 37.642980449744144 40.0 37.0 41.0 32.0 41.0 30-31 37.41382348711059 39.0 36.0 41.0 32.0 41.0 32-33 37.20517002283206 39.0 36.0 41.0 31.0 41.0 34-35 37.02851596527958 39.0 36.0 41.0 31.0 41.0 36-37 37.02859135029476 39.0 36.0 41.0 31.0 41.0 38-39 36.88742157098285 39.0 35.5 41.0 30.5 41.0 40-41 36.72104214369651 39.0 35.0 41.0 30.0 41.0 42-43 36.50212773694593 39.0 35.0 40.5 30.0 41.0 44-45 36.22806255199319 39.0 35.0 40.0 30.0 41.0 46-47 35.875877040298505 38.0 35.0 40.0 28.5 41.0 48-49 35.491357281661294 38.0 34.5 40.0 27.5 41.0 50-51 35.12946283375745 38.0 34.0 40.0 26.5 41.0 52-53 34.63552652653634 38.0 33.0 40.0 25.5 41.0 54-55 34.319960358513974 38.0 33.0 40.0 24.0 41.0 56-57 33.9591094516874 37.0 33.0 40.0 23.5 41.0 58-59 33.520663665975334 37.0 32.0 39.5 23.0 41.0 60-61 33.116373012409724 36.0 31.5 39.0 22.0 40.5 62-63 32.65040801884094 36.0 31.0 39.0 20.5 40.0 64-65 32.16924201490866 35.0 31.0 39.0 19.0 40.0 66-67 31.675171894178234 35.0 30.0 38.0 17.5 40.0 68-69 31.10911766436385 34.5 29.5 38.0 13.5 40.0 70-71 30.543638526196396 34.0 29.0 37.0 9.5 39.0 72-73 29.96314959819174 34.0 28.5 36.5 7.5 39.0 74-75 29.3535775807825 34.0 28.0 36.0 6.5 39.0 76-77 28.06277467521112 31.5 26.5 34.5 2.0 36.5 78-79 28.69193658179417 33.0 27.5 35.0 2.0 37.0 80-81 28.388017909354936 33.0 27.0 35.0 2.0 37.0 82-83 27.894223996051377 33.0 26.0 35.0 2.0 36.0 84-85 27.30830632547695 33.0 26.0 35.0 2.0 36.0 86-87 26.662994660534537 32.0 25.0 35.0 2.0 35.5 88-89 25.92134503210707 32.0 23.5 34.0 2.0 35.0 90-91 25.371087333846525 31.0 20.5 34.0 2.0 35.0 92-93 24.595392075625266 31.0 18.5 34.0 2.0 35.0 94-95 23.814845413730943 31.0 9.0 34.0 2.0 35.0 96-97 22.833134145285108 30.5 2.0 34.0 2.0 35.0 98-99 21.659832575805865 30.0 2.0 34.0 2.0 35.0 100-101 18.497329450085722 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38008258216298074 1101 2 0.4089936156248726 1101 3 0.3421815447349843 1101 4 0.2420137543745824 1101 5 0.21460428842064516 1101 6 0.27765319220170426 1101 7 0.2530711956012439 1101 8 0.26362744396338655 1101 9 0.34036724963925735 1101 10-11 0.30701009477012775 1101 12-13 0.4454128907643877 1101 14-15 0.5454589171262256 1101 16-17 0.4727215899924815 1101 18-19 0.5662461846483282 1101 20-21 0.6501245472841148 1101 22-23 0.5941536219301895 1101 24-25 0.5581738950920965 1101 26-27 0.45137322339767394 1101 28-29 0.4790580096971624 1101 30-31 0.5397375675637264 1101 32-33 0.44461066389744985 1101 34-35 0.37814250056010934 1101 36-37 0.5130313203261068 1101 38-39 0.5399425233818391 1101 40-41 0.760152925727354 1101 42-43 0.6400247433398718 1101 44-45 0.6384454899863101 1101 46-47 0.6334445060191456 1101 48-49 0.7294334879874356 1101 50-51 0.8051250035499464 1101 52-53 0.7197681059442758 1101 54-55 0.7819440351980589 1101 56-57 0.7979215388833225 1101 58-59 0.9363781623828729 1101 60-61 1.0175944246314046 1101 62-63 1.0478333771074162 1101 64-65 0.9960299247124524 1101 66-67 0.9979410993196502 1101 68-69 1.2064954342999634 1101 70-71 1.4248730042004567 1101 72-73 1.453101949692499 1101 74-75 1.5509608990015202 1101 76-77 1.4437819153276372 1101 78-79 1.6441688825817202 1101 80-81 1.7825557393118885 1101 82-83 1.967452971400963 1101 84-85 2.314031973067898 1101 86-87 2.3553765973176226 1101 88-89 2.4158271079617855 1101 90-91 2.6943090130385805 1101 92-93 2.78825610785308 1101 94-95 2.899458829432337 1101 96-97 3.230441831407031 1101 98-99 3.6035902711329904 1101 100-101 4.04312591716392 1102 1 0.3312483052907176 1102 2 0.2801529410379331 1102 3 0.3295237406389617 1102 4 0.1808895420002088 1102 5 0.1495625916872001 1102 6 0.1542952103087245 1102 7 0.260938152892777 1102 8 0.24033939057966336 1102 9 0.36711750815156563 1102 10-11 0.355627518871664 1102 12-13 0.34853047480878985 1102 14-15 0.6224476020982905 1102 16-17 0.41861180223293815 1102 18-19 0.4540546326073738 1102 20-21 0.4158244275129377 1102 22-23 0.43905665191761756 1102 24-25 0.42449959828277173 1102 26-27 0.42001084493565344 1102 28-29 0.35090825109930535 1102 30-31 0.37815156665614325 1102 32-33 0.298723985278464 1102 34-35 0.5000732713359497 1102 36-37 0.5495985372458279 1102 38-39 0.7004193391053377 1102 40-41 0.6298080442710585 1102 42-43 0.5550439364838979 1102 44-45 0.5354938001713236 1102 46-47 0.6038905978337965 1102 48-49 0.8763783932491904 1102 50-51 0.7115208584259705 1102 52-53 0.9201884209201339 1102 54-55 0.9096980281903271 1102 56-57 0.9254986648215819 1102 58-59 1.0381650589705131 1102 60-61 1.139323593002885 1102 62-63 1.3093630260514217 1102 64-65 1.2627192189552474 1102 66-67 1.3452698674620045 1102 68-69 1.428148722799694 1102 70-71 1.531150389766914 1102 72-73 1.45020530998055 1102 74-75 1.720659722863509 1102 76-77 1.6975208588120125 1102 78-79 1.946032774921573 1102 80-81 2.198361628185072 1102 82-83 2.0873654863723594 1102 84-85 2.3590328285762574 1102 86-87 2.4536045791362113 1102 88-89 2.4793978882598005 1102 90-91 2.71468387448342 1102 92-93 2.861737766497807 1102 94-95 3.1580875611225956 1102 96-97 3.5428022533586336 1102 98-99 3.8298236910090573 1102 100-101 4.20859090455447 1103 1 0.23860555921523385 1103 2 0.27561387880590615 1103 3 0.269905673570598 1103 4 0.22839677362958355 1103 5 0.08181179992382681 1103 6 0.09427125732288744 1103 7 0.14447604891738308 1103 8 0.14150616967481966 1103 9 0.16170564827939415 1103 10-11 0.3597499763679721 1103 12-13 0.37288112879961943 1103 14-15 0.5053591618435576 1103 16-17 0.5388544334026975 1103 18-19 0.41991126626961517 1103 20-21 0.4368286664482639 1103 22-23 0.3980057218100299 1103 24-25 0.41362893011451973 1103 26-27 0.4915979017482428 1103 28-29 0.4356494416598835 1103 30-31 0.5154957150388881 1103 32-33 0.642742313028478 1103 34-35 0.6407595472469083 1103 36-37 0.599034441571078 1103 38-39 0.5541114003197976 1103 40-41 0.46009449396978397 1103 42-43 0.5845667775709913 1103 44-45 0.7520789561801493 1103 46-47 0.6956776249195329 1103 48-49 0.8583786390674177 1103 50-51 0.8468430803941231 1103 52-53 0.9413109241500663 1103 54-55 0.8950532641186655 1103 56-57 1.0320672995585625 1103 58-59 1.1491659250510224 1103 60-61 0.9532002465592413 1103 62-63 1.077996619685301 1103 64-65 1.1081677943528447 1103 66-67 1.1765356598542205 1103 68-69 1.230763114027983 1103 70-71 1.2360834757575603 1103 72-73 1.1339894529895922 1103 74-75 1.3143371921933955 1103 76-77 1.1258594848595997 1103 78-79 1.4012345459561821 1103 80-81 1.7438808763242868 1103 82-83 2.0136671796598087 1103 84-85 2.2064558102942406 1103 86-87 2.373508209814325 1103 88-89 2.515846157813783 1103 90-91 2.621351193807019 1103 92-93 2.7977828655682693 1103 94-95 2.8543048187058027 1103 96-97 3.40468313441691 1103 98-99 3.801789693775941 1103 100-101 4.183133353933062 1104 1 0.19218299227130942 1104 2 0.22602772636297885 1104 3 0.16789259108696797 1104 4 0.16185750575858293 1104 5 0.057196208489379785 1104 6 0.14625643232322716 1104 7 0.13199357617823182 1104 8 0.20727253639276455 1104 9 0.14033004758783107 1104 10-11 0.18430532847873593 1104 12-13 0.2075239300302698 1104 14-15 0.43681829780064874 1104 16-17 0.38768478653445726 1104 18-19 0.36914883042279456 1104 20-21 0.41642923208529936 1104 22-23 0.36347344442325635 1104 24-25 0.3674606786204393 1104 26-27 0.37889786975674156 1104 28-29 0.38988380666254585 1104 30-31 0.46441625698859923 1104 32-33 0.34185948361808016 1104 34-35 0.4049058335096163 1104 36-37 0.42907293347791153 1104 38-39 0.5372980775595408 1104 40-41 0.5471242358595987 1104 42-43 0.6289761769474964 1104 44-45 0.5418738361779774 1104 46-47 0.40700049067773136 1104 48-49 0.6800955959265309 1104 50-51 0.7062375220402544 1104 52-53 0.8148769219446947 1104 54-55 0.934609739335464 1104 56-57 0.9181736270698977 1104 58-59 1.1453292416852392 1104 60-61 1.2230117519297394 1104 62-63 1.1908531207675637 1104 64-65 1.1473178243023412 1104 66-67 1.1948732587215822 1104 68-69 1.288252699772027 1104 70-71 1.4227108564020199 1104 72-73 1.533708165978144 1104 74-75 1.5679976670290152 1104 76-77 1.423440807946573 1104 78-79 1.7524844268388797 1104 80-81 1.9721174637155485 1104 82-83 2.138373810750103 1104 84-85 2.249482427907264 1104 86-87 2.471368607734199 1104 88-89 2.4561833618923608 1104 90-91 2.4867866696636405 1104 92-93 2.8820632949351115 1104 94-95 3.036806146012932 1104 96-97 3.3238278381211153 1104 98-99 3.6753277840872762 1104 100-101 3.960570579112421 1105 1 0.3040242185408779 1105 2 0.26077041137834556 1105 3 0.21561459130560223 1105 4 0.17549351973468674 1105 5 0.15739904824597062 1105 6 0.1398214321410407 1105 7 0.1934690026073156 1105 8 0.1938203332938926 1105 9 0.2964899387588389 1105 10-11 0.33611084523426626 1105 12-13 0.37788462851160887 1105 14-15 0.44302535630027506 1105 16-17 0.47401071479963264 1105 18-19 0.5085406350488313 1105 20-21 0.5062062703009573 1105 22-23 0.343227526522746 1105 24-25 0.38617335468818936 1105 26-27 0.5405716823749103 1105 28-29 0.5561379110298716 1105 30-31 0.5615433010748632 1105 32-33 0.5721486011234305 1105 34-35 0.6310855228125547 1105 36-37 0.7693453298616149 1105 38-39 0.7763862298553619 1105 40-41 0.7271107132091643 1105 42-43 0.721489325093394 1105 44-45 0.7526785958270281 1105 46-47 0.7807677634344543 1105 48-49 0.7912975328235348 1105 50-51 0.6956801378228832 1105 52-53 0.7581890805888705 1105 54-55 0.8411034456348219 1105 56-57 0.9177404164121015 1105 58-59 1.1881405764194213 1105 60-61 1.0759144657718238 1105 62-63 1.2664746970875456 1105 64-65 1.1671021906608985 1105 66-67 1.3371007712421807 1105 68-69 1.4197758603474249 1105 70-71 1.4832627748774385 1105 72-73 1.5585408535441836 1105 74-75 1.700653653231054 1105 76-77 1.5225209438208722 1105 78-79 1.7692148604948912 1105 80-81 1.9566429360973565 1105 82-83 2.009394851832237 1105 84-85 2.1646743564234576 1105 86-87 2.3581775456393217 1105 88-89 2.5443307992691295 1105 90-91 2.618633726057954 1105 92-93 2.7738573943630307 1105 94-95 3.049868054650023 1105 96-97 3.3086391485631133 1105 98-99 3.644757282089248 1105 100-101 4.3649342655326855 1106 1 0.1324186491917736 1106 2 0.11642252179166235 1106 3 0.08717391168922006 1106 4 0.07394296231294817 1106 5 0.041951893774431426 1106 6 0.13330519631999493 1106 7 0.13839622134058516 1106 8 -0.04025298348717854 1106 9 -0.014709613356444606 1106 10-11 0.13573963643236198 1106 12-13 0.13459437974084665 1106 14-15 0.03458005178735135 1106 16-17 0.09577693844988744 1106 18-19 0.17091831870590823 1106 20-21 0.3115821039994202 1106 22-23 0.20187364891211956 1106 24-25 0.13050055394820248 1106 26-27 0.1428464012298818 1106 28-29 0.356221889414563 1106 30-31 0.25604210833839147 1106 32-33 0.276113841374638 1106 34-35 0.271847900496212 1106 36-37 0.3318228139178956 1106 38-39 0.2426123142718879 1106 40-41 0.2019074611766385 1106 42-43 0.3261689901535121 1106 44-45 -0.10454719649077049 1106 46-47 -0.11966551634745315 1106 48-49 0.2952347802798414 1106 50-51 0.12983743298865136 1106 52-53 0.5027073611010167 1106 54-55 0.600322811780579 1106 56-57 0.37966169580453624 1106 58-59 0.8957996485191302 1106 60-61 0.6994588404371314 1106 62-63 0.9034836060987885 1106 64-65 0.5422080764503008 1106 66-67 0.6913021246329656 1106 68-69 0.5715369722699251 1106 70-71 0.8183316614944545 1106 72-73 1.0259487207366327 1106 74-75 1.4291357135007594 1106 76-77 1.353435943090627 1106 78-79 1.6314270215386593 1106 80-81 1.5716227326019059 1106 82-83 1.9501925247790304 1106 84-85 1.8991493089574156 1106 86-87 2.1730222464815405 1106 88-89 2.4538556520026837 1106 90-91 2.2856801210728044 1106 92-93 2.222843568356506 1106 94-95 2.709686223190989 1106 96-97 3.123550788697475 1106 98-99 3.4977403403629417 1106 100-101 3.911895221669191 1107 1 0.15264305715792403 1107 2 0.09737031394767115 1107 3 0.09985576252486084 1107 4 0.14496819436363495 1107 5 0.058450040850097196 1107 6 0.02710066891855689 1107 7 0.10279523142644109 1107 8 0.2399645571078466 1107 9 0.27721964322689985 1107 10-11 0.25328521583910657 1107 12-13 0.25530876039574935 1107 14-15 0.3660228801315597 1107 16-17 0.3919046272472144 1107 18-19 0.3897633977756172 1107 20-21 0.22834742978813694 1107 22-23 0.31487022788530084 1107 24-25 0.25269838170694214 1107 26-27 0.25222661307404337 1107 28-29 0.33305740796438243 1107 30-31 0.32038684403954676 1107 32-33 0.3480770866980265 1107 34-35 0.38416169821573476 1107 36-37 0.431756513721993 1107 38-39 0.5909692087010967 1107 40-41 0.6289272436360491 1107 42-43 0.7407465677120868 1107 44-45 0.8442760937078759 1107 46-47 0.8921488937390407 1107 48-49 0.9642755161329006 1107 50-51 0.9756574350773448 1107 52-53 1.191129194910637 1107 54-55 1.0796052664385272 1107 56-57 0.9564680992325307 1107 58-59 1.0493114227376878 1107 60-61 1.0126401580229825 1107 62-63 1.2176430493670267 1107 64-65 1.3583943436068537 1107 66-67 1.367830747645936 1107 68-69 1.4497592567309958 1107 70-71 1.3457060471495552 1107 72-73 1.4310749601072388 1107 74-75 1.3934116172562945 1107 76-77 1.453183480172843 1107 78-79 1.6772665655705303 1107 80-81 2.002640780812751 1107 82-83 2.032480482827939 1107 84-85 2.197806690431971 1107 86-87 2.4589850392546566 1107 88-89 2.654939399118863 1107 90-91 2.6347118850377775 1107 92-93 2.7922442873734283 1107 94-95 3.069648579237281 1107 96-97 3.3699454188563145 1107 98-99 3.608762708371753 1107 100-101 3.663575907899954 1108 1 0.2383898260729147 1108 2 0.2166762331454848 1108 3 0.14211703884671323 1108 4 0.14814080057903567 1108 5 0.11958002272393031 1108 6 -0.01893576344458836 1108 7 0.204784654366442 1108 8 0.15162259420131363 1108 9 0.12340085011478408 1108 10-11 0.24966216486878778 1108 12-13 0.36492609083057914 1108 14-15 0.3440678189388038 1108 16-17 0.33793923826313943 1108 18-19 0.4473231628007497 1108 20-21 0.3890739456231742 1108 22-23 0.34624955670831525 1108 24-25 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62-63 0.19678963426452611 2316 64-65 0.12673358445905336 2316 66-67 0.4378242744943286 2316 68-69 0.4581526682120014 2316 70-71 0.6241112013829664 2316 72-73 0.687845475498051 2316 74-75 0.8355121495293432 2316 76-77 0.8665505924424473 2316 78-79 0.8370016109096987 2316 80-81 1.061207622879781 2316 82-83 1.338648084324678 2316 84-85 1.434626453820389 2316 86-87 1.5645033738873195 2316 88-89 1.800978903776386 2316 90-91 1.9907390180604985 2316 92-93 1.8964815451859351 2316 94-95 2.36783045853306 2316 96-97 2.692766476875054 2316 98-99 2.907044883567547 2316 100-101 3.209005613485406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 32.0 5 91.0 6 173.0 7 444.0 8 1057.0 9 1791.0 10 2977.0 11 4626.0 12 6629.0 13 9427.0 14 12167.0 15 16064.0 16 20455.0 17 25270.0 18 24790.0 19 22041.0 20 21867.0 21 23647.0 22 25908.0 23 29039.0 24 33960.0 25 39566.0 26 46881.0 27 55829.0 28 67108.0 29 79372.0 30 94004.0 31 111543.0 32 131858.0 33 160158.0 34 200965.0 35 254394.0 36 323777.0 37 372236.0 38 214401.0 39 12878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.540157414039456 19.286713292427855 14.98326204089104 23.189867252641648 2 15.895598742462811 20.546736816890665 39.30747977921565 24.25018466143088 3 19.45671129038321 24.217014759984767 29.74767797050618 26.57859597912584 4 13.340655281690717 16.86560956037257 36.35102204919761 33.442713108739106 5 13.881314359358404 37.34620932913913 33.95953231194092 14.812943999561549 6 32.55300649332607 35.98995029900679 17.035297347918394 14.421745859748732 7 29.11594011038491 32.46822388818339 20.13478595558781 18.281050045843894 8 26.524983697224357 35.27736782494006 19.330965140661494 18.866683337174088 9 26.36015814100961 16.850941148205713 19.894943115979334 36.893957594805336 10-11 25.172568353166337 26.648357709864527 27.041810286356828 21.137263650612315 12-13 26.083317398289473 23.82274756112111 27.470258670706816 22.623676369882602 14-15 22.65211429430637 25.304461485407586 25.951179111527328 26.092245108758718 16-17 22.377030492319307 28.59752001686663 26.52588259713431 22.499566893679752 18-19 22.328775910789904 27.049389646961146 28.88886573540636 21.73296870684259 20-21 22.950120043997067 26.195700316576204 28.988418900432944 21.86576073899379 22-23 22.63178689861553 26.0960858629194 28.864002981078972 22.4081242573861 24-25 22.599796437617446 26.25004749925125 28.952458663393543 22.197697399737763 26-27 22.189410951999267 26.6401406855006 28.881554765206452 22.28889359729368 28-29 22.181744486883414 26.877025589228893 28.85595374097627 22.08527618291142 30-31 22.238416040296865 26.513257139308237 28.75870911435478 22.489617706040114 32-33 22.07763712425041 26.53663064989339 28.659311043098572 22.72642118275763 34-35 22.777687193588765 26.598793908982916 28.482100496142543 22.14141840128578 36-37 22.72662174605273 26.24294927441039 28.60343689481847 22.426992084718417 38-39 22.310442759945243 26.56894755123307 28.59373148356251 22.52687820525918 40-41 22.58272384441596 26.355357747758827 28.631364185955853 22.430554221869357 42-43 22.468954095803813 26.38436548634037 28.32586967656443 22.820810741291382 44-45 22.436447607748804 26.6236967390103 28.364892034123972 22.574963619116925 46-47 22.584347524795355 26.917334645183487 27.8407916209385 22.657526209082658 48-49 22.54622797082334 26.718124600602426 27.9536829563674 22.78196447220683 50-51 22.59830287697002 27.00644674671779 27.804077409991518 22.59117296632067 52-53 22.907994325490023 26.924871579890137 27.560495963939402 22.606638130680434 54-55 22.44469772543546 26.951108619614285 27.808414154326027 22.795779500624224 56-57 22.554869667684876 27.041830715900232 27.708385428669025 22.69491418774587 58-59 22.65922377541231 26.62907222088749 27.675861595563685 23.035842408136514 60-61 22.395069779148464 26.88554470882998 27.906817583789728 22.81256792823183 62-63 22.27937727482966 27.00877571466629 27.848327801012978 22.863519209491077 64-65 22.096308136351674 26.9063624135626 27.794557242763446 23.202772207322276 66-67 22.186724697560546 26.90473116311301 27.685590447456764 23.222953691869684 68-69 22.47218717414825 26.709383549777886 27.735438614842007 23.082990661231857 70-71 22.28003102021871 26.855962729975367 27.491791409458717 23.372214840347205 72-73 22.43630118137945 26.787447513938567 27.494309860259854 23.28194144442213 74-75 22.269387228103206 27.151496504913712 27.219772038982835 23.359344228000243 76-77 22.30757004473253 26.872183787441223 27.454241908675037 23.36600425915121 78-79 22.353025778815052 27.00475109461494 27.500085804082314 23.142137322487695 80-81 22.306618970473167 27.197351512910455 27.29727245054516 23.198757066071213 82-83 22.34061896175607 27.081078408659565 27.336224540036223 23.24207808954814 84-85 22.313073825360753 26.915240303080513 27.36472896146484 23.406956910093893 86-87 22.352512150624847 27.05228599271473 27.145323311834375 23.44987854482605 88-89 22.209247189558212 27.098364798582026 27.041223353996457 23.6511646578633 90-91 22.20539723794684 27.020593331299896 27.21884303808278 23.555166392670483 92-93 22.31291380175433 26.735720111071704 27.261230973904034 23.690135113269932 94-95 21.988889597113058 26.82045618353248 27.318303726839027 23.87235049251543 96-97 22.065418666718966 26.692873684950293 27.289457211547102 23.952250436783636 98-99 21.98627461555685 26.84319426534545 26.930203690719594 24.240327428378105 100-101 21.994673977774532 26.668212513802814 27.196034187056355 24.141079321366295 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 679.0 1 447.5 2 281.0 3 402.0 4 382.5 5 353.5 6 648.0 7 1434.0 8 2208.0 9 2493.0 10 2279.5 11 1904.5 12 1909.0 13 2085.0 14 2289.0 15 2483.0 16 2675.5 17 2885.0 18 3113.5 19 3413.0 20 3799.0 21 4382.5 22 5217.0 23 6292.0 24 7478.5 25 9163.0 26 11713.0 27 14968.5 28 18235.5 29 22150.0 30 26852.5 31 31457.5 32 36915.0 33 40292.0 34 42396.5 35 46240.0 36 50627.5 37 55344.0 38 59931.0 39 63474.5 40 67527.0 41 73834.5 42 79484.5 43 84781.5 44 89435.0 45 93381.0 46 95360.5 47 97783.5 48 101795.5 49 101473.0 50 98726.5 51 94471.0 52 89174.0 53 86017.0 54 84692.0 55 79446.5 56 70685.0 57 63890.5 58 58114.5 59 50870.0 60 42128.5 61 34229.0 62 28012.5 63 21506.0 64 16054.0 65 12562.0 66 9493.0 67 7300.5 68 5663.0 69 4149.5 70 3131.0 71 2542.5 72 2123.5 73 1804.0 74 1516.5 75 1229.5 76 850.0 77 520.0 78 310.0 79 184.0 80 103.0 81 50.5 82 33.0 83 18.0 84 7.5 85 4.5 86 4.5 87 4.5 88 2.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0429543407876648E-4 2 0.043596645632408774 3 3.2687269452602645E-4 4 0.0 5 0.10104452169535792 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011849135176568459 26-27 0.011256678417740034 28-29 0.0 30-31 0.0 32-33 4.698794983811629E-4 34-35 0.01458669399322393 36-37 0.003330015575483894 38-39 0.011685698829305445 40-41 0.006823467498230801 42-43 0.005413829003087313 44-45 0.0026762701864318414 46-47 8.171817363150661E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.0429543407876653E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6343634726301322E-4 68-69 1.6343634726301322E-4 70-71 0.0 72-73 2.0429543407876653E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.0859086815753306E-5 82-83 6.128863022362996E-4 84-85 0.0040654791381674535 86-87 0.0012666316912883523 88-89 2.0429543407876653E-5 90-91 7.150340192756829E-4 92-93 3.2687269452602645E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.558931060914361E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2447436.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.180056930264506 #Duplication Level Percentage of deduplicated Percentage of total 1 73.22831750727549 38.94286093944396 2 14.763109657319093 15.702060240879343 3 5.242692436711226 8.364200467565105 4 2.3521125277284205 5.003419125239431 5 1.3028017628422506 3.4641535958399925 6 0.7362053828162085 2.3490866502319885 7 0.4943580406278659 1.840299212116675 8 0.33969669826465015 1.4452071802189583 9 0.26511709851658555 1.2689048153068707 >10 1.1441284646723953 10.741167187987227 >50 0.07683846961983977 2.8121470421842116 >100 0.04931316484059065 4.8994579377651855 >500 0.003462253542623075 1.22138909152765 >1k 0.0016926572875022668 1.3569853345104246 >5k 1.538779352274788E-4 0.5886611791829182 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7898 0.3227050676708196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6489 0.2651346143474232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4467 0.18251754080597 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.08590868157533E-5 0.0 0.0 0.0 6 0.0 4.08590868157533E-5 0.0 0.0 0.0 7 0.0 4.08590868157533E-5 0.0 0.0 0.0 8 0.0 4.08590868157533E-5 0.0 0.0 0.0 9 0.0 4.08590868157533E-5 0.0 0.0 0.0 10-11 0.0 4.08590868157533E-5 0.0 0.0 4.08590868157533E-5 12-13 0.0 4.08590868157533E-5 0.0 0.0 8.17181736315066E-5 14-15 0.0 4.08590868157533E-5 0.0 4.08590868157533E-5 1.0214771703938326E-4 16-17 0.0 4.08590868157533E-5 0.0 1.4300680385513655E-4 1.225772604472599E-4 18-19 0.0 4.08590868157533E-5 0.0 1.634363472630132E-4 2.042954340787665E-4 20-21 0.0 4.08590868157533E-5 0.0 1.634363472630132E-4 2.042954340787665E-4 22-23 0.0 4.08590868157533E-5 0.0 3.6773178134177974E-4 2.042954340787665E-4 24-25 0.0 4.08590868157533E-5 0.0 7.150340192756827E-4 2.042954340787665E-4 26-27 0.0 4.08590868157533E-5 0.0 0.0015935043858143786 2.451545208945198E-4 28-29 0.0 4.08590868157533E-5 0.0 0.004228915485430466 2.451545208945198E-4 30-31 0.0 8.17181736315066E-5 0.0 0.0120738601540551 2.451545208945198E-4 32-33 0.0 1.225772604472599E-4 0.0 0.030930328719525252 2.451545208945198E-4 34-35 0.0 1.225772604472599E-4 0.0 0.05987899172848647 2.451545208945198E-4 36-37 0.0 1.225772604472599E-4 0.0 0.1109528502481781 2.451545208945198E-4 38-39 0.0 1.4300680385513655E-4 0.0 0.1889119878926354 2.451545208945198E-4 40-41 0.0 1.634363472630132E-4 0.0 0.26956782526693246 2.451545208945198E-4 42-43 0.0 1.634363472630132E-4 0.0 0.3498150717730719 2.451545208945198E-4 44-45 0.0 1.634363472630132E-4 0.0 0.45457777036866337 2.451545208945198E-4 46-47 0.0 1.634363472630132E-4 0.0 0.578360373876988 2.451545208945198E-4 48-49 0.0 1.634363472630132E-4 0.0 0.7126233331535533 2.451545208945198E-4 50-51 0.0 2.042954340787665E-4 0.0 0.8594504616259628 2.451545208945198E-4 52-53 0.0 2.042954340787665E-4 0.0 0.999699277121036 2.451545208945198E-4 54-55 0.0 2.2472497748664315E-4 0.0 1.1557401296703977 2.451545208945198E-4 56-57 0.0 2.451545208945198E-4 0.0 1.3370523274153032 2.6558406430239644E-4 58-59 0.0 3.268726945260264E-4 0.0 1.533339380478182 2.860136077102731E-4 60-61 0.0 3.268726945260264E-4 0.0 1.7507710109682133 2.860136077102731E-4 62-63 0.0 3.268726945260264E-4 0.0 1.9723089796832278 2.860136077102731E-4 64-65 0.0 3.6773178134177974E-4 0.0 2.2030402429317864 2.860136077102731E-4 66-67 0.0 3.6773178134177974E-4 0.0 2.47279193408939 2.860136077102731E-4 68-69 0.0 4.08590868157533E-4 0.0 2.7666913455551034 2.860136077102731E-4 70-71 0.0 4.08590868157533E-4 0.0 3.07527959873108 2.860136077102731E-4 72-73 0.0 4.08590868157533E-4 0.0 3.3930611464406013 3.268726945260264E-4 74-75 0.0 4.08590868157533E-4 0.0 3.7168694094554464 3.8816132474965635E-4 76-77 0.0 4.08590868157533E-4 0.0 4.062986733871693 4.08590868157533E-4 78-79 0.0 4.08590868157533E-4 0.0 4.454232919675938 4.08590868157533E-4 80-81 0.0 4.08590868157533E-4 0.0 4.874591204836408 4.08590868157533E-4 82-83 0.0 4.08590868157533E-4 0.0 5.305675000286014 4.08590868157533E-4 84-85 0.0 4.08590868157533E-4 0.0 5.749057380867161 4.08590868157533E-4 86-87 0.0 4.08590868157533E-4 0.0 6.204758776123257 4.08590868157533E-4 88-89 0.0 4.08590868157533E-4 0.0 6.693065722658325 4.08590868157533E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6550 0.0 34.70907 1 GTATCAA 10045 0.0 32.95457 1 ATCAACG 12475 0.0 25.989378 3 TATCAAC 12630 0.0 25.795477 2 GTACATG 9930 0.0 25.38534 1 TCAACGC 12775 0.0 25.379059 4 CAACGCA 12885 0.0 25.162395 5 TACATGG 9740 0.0 24.855082 2 AACGCAG 13260 0.0 24.49995 6 ACATGGG 10065 0.0 23.096552 3 ACGCAGA 14930 0.0 21.695875 7 CGCAGAG 15005 0.0 21.555779 8 AGTACTT 8255 0.0 20.36755 12-13 AGAGTAC 13195 0.0 20.229239 10-11 GAGTACT 8060 0.0 20.035328 12-13 GCAGAGT 16130 0.0 19.55178 9 GTACTTT 8275 0.0 19.25649 14-15 CATGGGA 5370 0.0 19.033394 4 CATGGGG 4990 0.0 18.767988 4 TAAGGTG 1135 0.0 18.4293 5 >>END_MODULE