FastQCFastQC Report
Thu 26 May 2016
SRR938636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442496
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75050.3072676475212242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.2324671156063306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.15729810816476258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.039.1066321
GTATCAA97250.035.8682251
ATCAACG119000.029.1184543
TCAACGC121350.028.5545644
TATCAAC121900.028.3867382
CAACGCA122250.028.2660055
AACGCAG126150.027.4596216
GTACATG98700.027.170731
TACATGG97050.026.7414462
ACATGGG102800.024.1359923
ACGCAGA143150.024.059437
CGCAGAG144700.023.8673698
GCAGAGT151050.022.5157539
GAGTACT75150.022.153612-13
CGACGAG3450.022.04820494-95
GTACTTT78200.021.38285314-15
AGAGTAC124800.020.72376310-11
AGTACTT76950.020.24652512-13
CATGGGG51550.019.8242864
GGACCGA8200.019.1229296
Produced by FastQC (version 0.11.5)