FastQCFastQC Report
Thu 26 May 2016
SRR938636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442496
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75630.30964226758201446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.2541252882297453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43010.1760903600251546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64650.039.2466351
GTATCAA98550.033.2676541
ATCAACG123150.026.4983373
TATCAAC123650.026.3543912
TCAACGC125000.026.1050934
CAACGCA126400.025.8159565
AACGCAG130600.025.2039496
GTACATG96200.024.8934541
TACATGG95500.024.5226022
ACATGGG100700.022.6888223
ACGCAGA148750.022.0647817
GAGTACT78550.021.79905112-13
CGCAGAG150450.021.6891778
GTACTTT81250.020.92850314-15
AGTACTT80350.020.6013412-13
GCAGAGT159600.020.1481119
AGAGTAC131950.019.97846810-11
GGACCGA8100.019.9375786
GTAAGGT10850.019.2620054
GTATAGA9550.018.9064461
Produced by FastQC (version 0.11.5)