##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2442496 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81786582250288 31.0 31.0 34.0 30.0 34.0 2 32.07928733557803 33.0 31.0 34.0 30.0 34.0 3 32.13577913740698 33.0 31.0 34.0 30.0 34.0 4 35.782450411382456 37.0 35.0 37.0 35.0 37.0 5 35.67706026949481 37.0 35.0 37.0 33.0 37.0 6 35.70952050689131 37.0 35.0 37.0 33.0 37.0 7 35.63116050138874 37.0 35.0 37.0 33.0 37.0 8 35.60737254022115 37.0 35.0 37.0 33.0 37.0 9 37.154525125117914 39.0 37.0 39.0 33.0 39.0 10-11 37.08623330396447 39.0 37.0 39.0 33.0 39.0 12-13 36.967041501807984 39.0 37.0 39.0 33.0 39.0 14-15 37.98326732162509 40.0 37.0 41.0 33.0 41.0 16-17 38.07812008699298 40.0 37.0 41.0 33.0 41.0 18-19 38.219398516926944 40.0 37.5 41.0 33.0 41.0 20-21 38.17507439111466 40.0 37.5 41.0 33.0 41.0 22-23 38.04959189288334 40.0 37.0 41.0 33.0 41.0 24-25 37.93260152729012 40.0 37.0 41.0 32.5 41.0 26-27 37.71781837104339 40.0 37.0 41.0 32.0 41.0 28-29 37.5788568333377 39.5 37.0 41.0 32.0 41.0 30-31 37.328635133895816 39.0 36.0 41.0 31.5 41.0 32-33 37.22431500399591 39.0 36.0 41.0 31.0 41.0 34-35 37.08289552981867 39.0 36.0 41.0 31.0 41.0 36-37 36.956108014097055 39.0 36.0 41.0 31.0 41.0 38-39 36.74771115285347 39.0 35.0 41.0 30.0 41.0 40-41 36.532676000288234 39.0 35.0 40.0 30.0 41.0 42-43 36.27666186556703 39.0 35.0 40.0 30.0 41.0 44-45 35.98381266540457 38.0 35.0 40.0 29.5 41.0 46-47 35.57009796536002 38.0 34.5 40.0 27.5 41.0 48-49 35.16434192727439 38.0 34.0 40.0 26.5 41.0 50-51 34.769215998716064 38.0 33.5 40.0 25.5 41.0 52-53 34.18127235418195 37.5 33.0 40.0 24.0 41.0 54-55 33.7707793584923 37.0 33.0 40.0 23.0 41.0 56-57 33.376960494510534 37.0 32.0 39.5 22.5 41.0 58-59 32.8501090687559 36.0 31.0 39.0 20.5 40.0 60-61 32.37149907307934 36.0 31.0 39.0 19.0 40.0 62-63 31.8774812732549 35.0 30.5 39.0 16.5 40.0 64-65 31.230787481330573 35.0 29.5 38.0 12.0 40.0 66-67 30.580032270267793 34.0 28.5 38.0 8.0 40.0 68-69 29.85730314399696 34.0 28.0 37.0 7.0 39.0 70-71 29.08654466578451 33.5 26.5 36.5 2.0 39.0 72-73 28.507255078411593 33.0 26.0 36.0 2.0 39.0 74-75 27.792954420396185 32.5 25.5 35.0 2.0 38.0 76-77 26.64500146571376 31.0 24.5 34.0 2.0 36.0 78-79 27.150104647049574 32.0 25.5 35.0 2.0 37.0 80-81 26.7618229466906 32.0 25.0 35.0 2.0 36.0 82-83 26.080430019127974 31.5 23.0 34.5 2.0 36.0 84-85 25.304859250537156 31.0 20.5 34.0 2.0 35.5 86-87 24.406074769416204 31.0 17.0 34.0 2.0 35.0 88-89 23.434927836115186 30.0 8.5 34.0 2.0 35.0 90-91 22.572932770411906 29.0 2.0 34.0 2.0 35.0 92-93 21.485942453948745 29.0 2.0 34.0 2.0 35.0 94-95 20.310981061995598 27.5 2.0 33.0 2.0 34.5 96-97 18.929826292448382 25.5 2.0 33.0 2.0 34.0 98-99 17.150077011385072 21.0 2.0 32.0 2.0 34.0 100-101 12.868988321782307 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3424578668792506 1101 2 0.31027670992874334 1101 3 0.3041672879826933 1101 4 0.2240807200222008 1101 5 0.15597067479212257 1101 6 0.19633173695754635 1101 7 0.30141338153413244 1101 8 0.2519864937937868 1101 9 0.292572525902024 1101 10-11 0.3867513386785433 1101 12-13 0.33590465060247254 1101 14-15 0.3634821167357458 1101 16-17 0.31446537268949015 1101 18-19 0.3346462692819827 1101 20-21 0.39461798683990423 1101 22-23 0.3780023730706432 1101 24-25 0.357521078011267 1101 26-27 0.3294509037029414 1101 28-29 0.4467112764943053 1101 30-31 0.5612077606253578 1101 32-33 0.5885806373569622 1101 34-35 0.3755145778200202 1101 36-37 0.3518840625474482 1101 38-39 0.5440256402042891 1101 40-41 0.6137842896885743 1101 42-43 0.7079297170868344 1101 44-45 0.7497485647367412 1101 46-47 0.755031478426055 1101 48-49 0.8139888432157747 1101 50-51 0.698332348066792 1101 52-53 0.8436134160686777 1101 54-55 0.9371842641394963 1101 56-57 0.9609527298697671 1101 58-59 1.0985822301126902 1101 60-61 1.0820376827681315 1101 62-63 1.1326708308554458 1101 64-65 1.0309356222557646 1101 66-67 1.3078805082169538 1101 68-69 1.3290762663120255 1101 70-71 1.6020472506302177 1101 72-73 1.5934131156959488 1101 74-75 1.6427358913114105 1101 76-77 1.5678761577752525 1101 78-79 1.4362446857505766 1101 80-81 1.5511264815191659 1101 82-83 1.7173420516061206 1101 84-85 1.9584723202726977 1101 86-87 2.110976927176175 1101 88-89 1.9526105757017866 1101 90-91 2.3563729163074356 1101 92-93 2.6447620529478932 1101 94-95 2.5541618274149656 1101 96-97 2.6342685391680902 1101 98-99 2.765534216693542 1101 100-101 2.461345456560492 1102 1 0.2567479257119203 1102 2 0.20066504053814072 1102 3 0.20354534056087914 1102 4 0.16837930437709758 1102 5 0.189157856315731 1102 6 0.1509130929750384 1102 7 0.20602635272603465 1102 8 0.1928548134928434 1102 9 0.24271864124671794 1102 10-11 0.31239478223104555 1102 12-13 0.24059784484363433 1102 14-15 0.404755234648313 1102 16-17 0.3390338638470851 1102 18-19 0.35621952350080477 1102 20-21 0.23116826444811522 1102 22-23 0.3460865445525201 1102 24-25 0.32972014494427526 1102 26-27 0.3923435864127569 1102 28-29 0.3367305810771626 1102 30-31 0.4578990703541521 1102 32-33 0.37018031413099806 1102 34-35 0.42046191138818756 1102 36-37 0.45503978204974516 1102 38-39 0.4825096944146523 1102 40-41 0.579549468071825 1102 42-43 0.6228309296946932 1102 44-45 0.579609333988266 1102 46-47 0.6162167958577172 1102 48-49 0.8179620720549465 1102 50-51 0.9579899269205754 1102 52-53 0.9654162540684368 1102 54-55 0.9462717123682793 1102 56-57 1.048204229858463 1102 58-59 1.3322477233349304 1102 60-61 1.1689248971831958 1102 62-63 1.3179262719837652 1102 64-65 1.41407197153816 1102 66-67 1.6585064667912306 1102 68-69 1.6312454975637145 1102 70-71 1.8600879703914153 1102 72-73 1.8016085200821053 1102 74-75 1.6406442576273577 1102 76-77 1.6761419031895954 1102 78-79 1.8530778152444363 1102 80-81 2.0626517988195587 1102 82-83 2.2174742373122704 1102 84-85 2.366074329349569 1102 86-87 2.2018920679639784 1102 88-89 1.8993692727919473 1102 90-91 2.078214261370835 1102 92-93 2.3129589742789847 1102 94-95 2.1654694849967377 1102 96-97 2.4845643069646606 1102 98-99 2.5329435300061185 1102 100-101 2.4497530436189088 1103 1 0.2568154982732409 1103 2 0.25811122004887466 1103 3 0.21933338796021928 1103 4 0.19341564222582974 1103 5 0.15962020045012082 1103 6 0.19534416368338725 1103 7 0.2679914472748308 1103 8 0.2391191418673415 1103 9 0.25804635057208714 1103 10-11 0.3017934568234111 1103 12-13 0.42468045933864573 1103 14-15 0.4890662584782035 1103 16-17 0.35492608561177974 1103 18-19 0.43681247272635915 1103 20-21 0.3758055722446514 1103 22-23 0.37173121142330956 1103 24-25 0.3518858807285241 1103 26-27 0.5151521413805753 1103 28-29 0.47202123107592797 1103 30-31 0.5134567375522536 1103 32-33 0.5287389773204936 1103 34-35 0.5553487097509588 1103 36-37 0.5413843176467594 1103 38-39 0.5193033059920822 1103 40-41 0.5439067016793118 1103 42-43 0.6076744393853701 1103 44-45 0.6309231275892841 1103 46-47 0.7282251790786063 1103 48-49 0.6961852304386937 1103 50-51 0.7478512271996678 1103 52-53 0.8692323440807215 1103 54-55 0.9699120785270949 1103 56-57 1.1797815564486172 1103 58-59 1.145685336615749 1103 60-61 1.0091113904136222 1103 62-63 1.0723740950562757 1103 64-65 1.3441952562685309 1103 66-67 1.245162469663061 1103 68-69 1.0127804153328341 1103 70-71 1.3313346137596263 1103 72-73 1.5487512917537174 1103 74-75 1.4670318707322103 1103 76-77 1.4662217584716934 1103 78-79 1.8653288613873436 1103 80-81 1.9408158312121557 1103 82-83 2.0694709713051402 1103 84-85 2.203318600639328 1103 86-87 2.186258874350994 1103 88-89 2.170924743881802 1103 90-91 2.384829333571414 1103 92-93 2.753697486063711 1103 94-95 2.2876023143513855 1103 96-97 2.3179275552339966 1103 98-99 2.4564353773062066 1103 100-101 2.265882121288641 1104 1 0.2347365123864904 1104 2 0.30062119064066195 1104 3 0.25760938199800165 1104 4 0.15143945079046972 1104 5 0.04997457609636058 1104 6 0.13372825639310548 1104 7 0.18712457649638736 1104 8 0.2114683744339132 1104 9 0.1394943356396965 1104 10-11 0.3078857569751605 1104 12-13 0.27485737859363724 1104 14-15 0.4591538116882603 1104 16-17 0.469465692696609 1104 18-19 0.4596713133177488 1104 20-21 0.41504035450304144 1104 22-23 0.48003768794374224 1104 24-25 0.4268605346113432 1104 26-27 0.4788230984230992 1104 28-29 0.49501687355803625 1104 30-31 0.47828052328859627 1104 32-33 0.5743937128480283 1104 34-35 0.4589607832311984 1104 36-37 0.5285655608486337 1104 38-39 0.6321757764160196 1104 40-41 0.5346738329688421 1104 42-43 0.48282366255205744 1104 44-45 0.6824764585055121 1104 46-47 0.6038955922706677 1104 48-49 0.7000104798713451 1104 50-51 0.8877765652597986 1104 52-53 0.9555397337327918 1104 54-55 0.9465202980546863 1104 56-57 1.0246524087495743 1104 58-59 1.052194588787799 1104 60-61 1.0178625150426654 1104 62-63 1.0761568771535224 1104 64-65 1.2699195509414771 1104 66-67 1.1376021089760613 1104 68-69 1.4563163161774817 1104 70-71 1.4955692281629354 1104 72-73 1.5439847110288554 1104 74-75 1.7036167248325746 1104 76-77 1.5965780140948382 1104 78-79 1.583472484908544 1104 80-81 1.896605824363668 1104 82-83 1.9633790068635584 1104 84-85 2.1179374353222116 1104 86-87 1.921886283071398 1104 88-89 1.8121084914814745 1104 90-91 2.1106189238707316 1104 92-93 2.4994826449888983 1104 94-95 2.3320954927209065 1104 96-97 2.3751871196164878 1104 98-99 2.5358931046826996 1104 100-101 2.1819171492868694 1105 1 0.0836293115791591 1105 2 0.11229828193814484 1105 3 0.08073563785225701 1105 4 0.08234729654179063 1105 5 0.020636412086439293 1105 6 0.05514988020224365 1105 7 0.01176105911837766 1105 8 -0.0014010820381358258 1105 9 0.1511295171038327 1105 10-11 0.16225236780473296 1105 12-13 0.099205711100943 1105 14-15 0.1512891064914399 1105 16-17 0.08872750332947277 1105 18-19 0.24234705607268836 1105 20-21 0.17967309757687389 1105 22-23 0.29889099776401906 1105 24-25 0.13056625714455805 1105 26-27 0.0414919864069887 1105 28-29 0.1337385019775752 1105 30-31 0.3061006644693478 1105 32-33 0.2992110737003415 1105 34-35 0.3048112775046974 1105 36-37 0.27979980383985037 1105 38-39 0.18529400889040915 1105 40-41 0.23163812348563084 1105 42-43 0.2487358721767805 1105 44-45 0.2912398398766669 1105 46-47 0.3686626453146218 1105 48-49 0.33361616308807385 1105 50-51 0.5107555123720857 1105 52-53 0.4614152746545841 1105 54-55 0.5068329185174392 1105 56-57 0.5981466162919347 1105 58-59 0.6872635943369332 1105 60-61 0.7505971204627002 1105 62-63 0.6760930999281385 1105 64-65 1.0865850810160218 1105 66-67 1.1908009434408662 1105 68-69 1.2003975527384796 1105 70-71 1.0935325031106515 1105 72-73 1.1553860266582454 1105 74-75 1.2039825954365249 1105 76-77 1.1504396738651117 1105 78-79 1.2441369046446482 1105 80-81 1.4948222712038124 1105 82-83 1.7155013124999066 1105 84-85 1.8360350554583995 1105 86-87 1.8121227806771927 1105 88-89 1.7008960742606014 1105 90-91 1.8300197829422729 1105 92-93 2.0480537085701833 1105 94-95 1.7509880389818093 1105 96-97 1.9887461993546687 1105 98-99 2.0775121465387443 1105 100-101 2.126674690175369 1106 1 -0.05175742683336182 1106 2 -0.04333574239393556 1106 3 -0.029630904476782405 1106 4 0.03976263495009391 1106 5 -0.017471470513243048 1106 6 -0.103881613199583 1106 7 -0.05812589745087848 1106 8 0.026278987022713807 1106 9 -0.017906901410526643 1106 10-11 0.017858995710533065 1106 12-13 -0.06885731472897305 1106 14-15 -0.05801935619439291 1106 16-17 -0.17964960574654754 1106 18-19 -0.05910034942329645 1106 20-21 -0.03487682440965756 1106 22-23 0.049460429752585355 1106 24-25 0.005290907449854387 1106 26-27 -0.3141770834960482 1106 28-29 -0.031677607589394086 1106 30-31 -0.01721673834943971 1106 32-33 -0.19178775629274014 1106 34-35 -0.3705587108420687 1106 36-37 -0.17118166727799178 1106 38-39 0.006588369051165444 1106 40-41 -0.09267124851178465 1106 42-43 0.024182742232646603 1106 44-45 0.10144535990532688 1106 46-47 -0.38636311591185546 1106 48-49 -0.12291827484419571 1106 50-51 0.18957947979609457 1106 52-53 0.08566985379943048 1106 54-55 0.05912083232250609 1106 56-57 0.12219482463590481 1106 58-59 0.5590234986133495 1106 60-61 0.3907338216051528 1106 62-63 0.28649179988575213 1106 64-65 0.46263584323768114 1106 66-67 0.626601984505065 1106 68-69 0.5968110405220948 1106 70-71 0.491730973437825 1106 72-73 0.6398477259079272 1106 74-75 0.4319025198192641 1106 76-77 0.37644052151522445 1106 78-79 0.7696832526073614 1106 80-81 0.8691298089675641 1106 82-83 1.0952715883920625 1106 84-85 0.9547770209295336 1106 86-87 1.0065449931688697 1106 88-89 1.1158873831151084 1106 90-91 1.0960400188325323 1106 92-93 1.0919593306134594 1106 94-95 0.9000181196334509 1106 96-97 0.8877258971197897 1106 98-99 1.1538008908223745 1106 100-101 1.3322836206298145 1107 1 0.020732662412250136 1107 2 0.08489477296529202 1107 3 -0.015615242555561792 1107 4 0.09109350768233782 1107 5 -0.0072931745771356304 1107 6 -0.08500333483920741 1107 7 -0.041512934379419164 1107 8 0.023052865594003435 1107 9 -0.01757882126523924 1107 10-11 0.05687905156226947 1107 12-13 0.07229037992215126 1107 14-15 0.14421568979541632 1107 16-17 0.1151811791070898 1107 18-19 0.07216197799287016 1107 20-21 -0.011945389254300665 1107 22-23 0.006533681456460272 1107 24-25 0.12672352408430498 1107 26-27 0.23465706027335642 1107 28-29 0.047049910132791695 1107 30-31 0.18053649851270137 1107 32-33 0.17782772596554963 1107 34-35 0.13900638767962192 1107 36-37 0.2502841479425868 1107 38-39 0.12644209998638445 1107 40-41 0.25494821757683894 1107 42-43 0.2369319757358781 1107 44-45 0.22960752523428596 1107 46-47 0.34497927868262934 1107 48-49 0.5355551760511972 1107 50-51 0.5107582248895355 1107 52-53 0.3496269299804169 1107 54-55 0.4302400152467172 1107 56-57 0.3674610968252523 1107 58-59 0.49168211950502183 1107 60-61 0.4825757572780134 1107 62-63 0.7089477141162348 1107 64-65 0.8389213120212453 1107 66-67 0.7309431878561696 1107 68-69 0.8633312428381821 1107 70-71 0.8303174948118546 1107 72-73 1.1555731150148816 1107 74-75 0.952812746936651 1107 76-77 1.1451703826670219 1107 78-79 1.3216663799141735 1107 80-81 1.417641731165702 1107 82-83 1.469668643482187 1107 84-85 1.6290461638201563 1107 86-87 1.8903392009920204 1107 88-89 1.9378912107168027 1107 90-91 2.1469832492502476 1107 92-93 2.0233856981022775 1107 94-95 1.7180879147786463 1107 96-97 1.6854303125317465 1107 98-99 1.6752989282000748 1107 100-101 1.5036332407989317 1108 1 0.025894430218755815 1108 2 0.08008885560693102 1108 3 0.08959930758263113 1108 4 0.0875322780261314 1108 5 0.01850619805895093 1108 6 -0.002596776060769912 1108 7 0.04957086761225327 1108 8 0.03954189389709484 1108 9 0.10965413671113566 1108 10-11 0.06692461935242733 1108 12-13 0.008382235522319093 1108 14-15 0.16426415487660506 1108 16-17 0.06109076354662335 1108 18-19 -0.044736471956419166 1108 20-21 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12 7436.0 13 10369.0 14 14152.0 15 18890.0 16 23526.0 17 29492.0 18 30100.0 19 27725.0 20 28664.0 21 31363.0 22 34841.0 23 38943.0 24 44231.0 25 50394.0 26 58392.0 27 68470.0 28 80659.0 29 94399.0 30 109378.0 31 127225.0 32 150098.0 33 179604.0 34 217451.0 35 265900.0 36 310219.0 37 280761.0 38 95335.0 39 1965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52719828394656 19.265754856295267 15.003591492135984 23.203455367622187 2 15.907751762452845 20.580437336443104 39.3171628941693 24.194648006934752 3 19.469025824027803 24.22788719869443 29.751348438906117 26.551738538371655 4 13.360390354784613 16.94315978408972 36.280714482234565 33.41573537889111 5 13.883864417049812 37.41259943745931 33.99534900859369 14.708187136897182 6 32.540442236138766 36.01778672308982 17.040805798658422 14.400965242112987 7 29.02453064406247 32.41847683680955 20.194751598364952 18.362240920763025 8 26.561036972440068 35.240317789801004 19.36331497096207 18.835330266796863 9 26.363161437290888 16.854112452732544 19.805329328667046 36.977396781309515 10-11 25.17265125510953 26.68012557645949 27.000105629650978 21.14711753878 12-13 26.12591791347867 23.788104463630646 27.47285973037418 22.613117892516506 14-15 22.622657314484854 25.357359848286343 25.909070066030814 26.110912771197984 16-17 22.384642595115817 28.599309886280267 26.515498899486428 22.500548619117495 18-19 22.331479764961745 27.03001355989938 28.892902997589353 21.745603677549525 20-21 22.961102085735245 26.18204901870873 28.981378065716385 21.87547082983964 22-23 22.626751487003457 26.137381596530762 28.848563109212872 22.38730380725291 24-25 22.575666696008728 26.2856429289383 28.959428116382902 22.179262258670068 26-27 22.20065327831683 26.649926964262132 28.867920472424018 22.281499284997018 28-29 22.161387255595365 26.8759584219638 28.865566164713368 22.09708815772747 30-31 22.270619890472695 26.46737190153024 28.722012236662824 22.539995971334243 32-33 22.061418520787 26.53515595573039 28.65340587139016 22.75001965209246 34-35 22.774676601168668 26.608190845949025 28.465341365774282 22.15179118710802 36-37 22.73708396589024 26.255574279785854 28.559985228056213 22.447356526267694 38-39 22.305438841415015 26.601897740191767 28.555713949661794 22.536949468731425 40-41 22.64219818349438 26.415389462809706 28.55176844477138 22.390643908924538 42-43 22.449235281870624 26.408667317264108 28.28497969493162 22.857117705933643 44-45 22.433894559240084 26.61486959670712 28.327431887378424 22.623803956674372 46-47 22.61751254711116 26.858504696735242 27.80231439620437 22.721668359949224 48-49 22.552442255790798 26.699163478671 27.91724121554344 22.83115304999476 50-51 22.609903967089405 26.984834366156583 27.743218412640186 22.662043254113826 52-53 22.903476607535897 26.926062519652028 27.480085944869508 22.690374927942564 54-55 22.446239420658213 26.973043149303006 27.75713859920344 22.82357883083534 56-57 22.6022888062048 27.02993167644901 27.628827232470393 22.7389522848758 58-59 22.650907104863222 26.649112219631064 27.631119969080807 23.068860706424903 60-61 22.42527725736296 26.87490583403207 27.90663730872026 22.79317959988471 62-63 22.335144049365894 26.984691070118437 27.819636142699927 22.86052873781574 64-65 22.12856569380197 26.905464884363095 27.75439207744378 23.211577344391156 66-67 22.19112911645312 26.94153700022723 27.60860353287738 23.258730350442274 68-69 22.528759726802043 26.70748723222891 27.668253306682804 23.095499734286246 70-71 22.35009692957406 26.776881835991478 27.47010741066 23.40291382377446 72-73 22.430763832750287 26.784269259483608 27.44683041885714 23.33813648890897 74-75 22.327876893145373 27.110627816790693 27.148294203961846 23.413201086102088 76-77 22.278132697044335 26.849910910805995 27.424528023792057 23.447428368357613 78-79 22.355819620584843 26.921272337805263 27.478714397075777 23.244193644534118 80-81 22.366380429184268 27.124631626526003 27.280496901937116 23.228491042352612 82-83 22.368729196264436 27.053556440816056 27.27896350825189 23.29875085466761 84-85 22.324800791694738 26.89739474863508 27.2897618712755 23.488042588394688 86-87 22.336085751807396 26.994254620715914 27.094872968057 23.57478665941969 88-89 22.19821444948119 27.081866254847498 26.929829158369145 23.79009013730217 90-91 22.205092438395727 26.98393364570908 27.12260505730994 23.68836885858525 92-93 22.303633068179447 26.696870927552762 27.151388281014267 23.848107723253527 94-95 21.98348410129806 26.735551147494675 27.165541792306634 24.11542295890063 96-97 22.050476234147364 26.663933124148414 27.14890832983964 24.136682311864586 98-99 21.971438233675716 26.753308910229535 26.797096085316003 24.478156770778746 100-101 22.03267109396926 26.56064258869668 27.03155440818622 24.375131909147836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 591.0 1 429.0 2 308.0 3 373.0 4 359.0 5 383.5 6 642.0 7 1291.0 8 2014.0 9 2320.0 10 2040.5 11 1737.5 12 1830.0 13 1993.5 14 2182.0 15 2369.5 16 2604.5 17 2862.5 18 3156.5 19 3473.5 20 3813.0 21 4375.5 22 5185.5 23 6259.0 24 7622.5 25 9400.5 26 11692.0 27 14921.0 28 18448.5 29 22189.5 30 26416.0 31 31331.5 32 36997.5 33 40164.0 34 42101.5 35 45769.5 36 50363.5 37 55027.0 38 59561.0 39 63329.0 40 67388.0 41 73582.5 42 79166.5 43 84323.0 44 88706.5 45 92587.0 46 94796.0 47 97326.5 48 100964.5 49 100869.5 50 98205.0 51 94070.0 52 89566.5 53 86220.5 54 84368.5 55 79299.5 56 71131.0 57 64225.5 58 58220.0 59 50894.0 60 42192.0 61 34804.5 62 28587.0 63 21894.5 64 16533.5 65 12866.0 66 9665.5 67 7473.5 68 5796.5 69 4312.0 70 3343.5 71 2659.0 72 2125.0 73 1829.5 74 1565.5 75 1244.5 76 834.5 77 485.0 78 295.0 79 200.0 80 122.0 81 60.0 82 28.0 83 11.0 84 8.0 85 7.5 86 4.5 87 2.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025138219264228066 2 0.012405342731369878 3 0.0017195524578136464 4 0.0 5 2.456503511162352E-4 6 0.0 7 0.0 8 4.0941725186039196E-5 9 4.913007022324704E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016786107326276073 26-27 0.008126932449428782 28-29 1.842377633371764E-4 30-31 0.0 32-33 3.2753380148831357E-4 34-35 0.009539421968347134 36-37 0.0011463683052090976 38-39 0.0112794452887538 40-41 0.011443212189497957 42-43 0.005138186510847919 44-45 6.345967403836076E-4 46-47 6.14125877790588E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.14125877790588E-5 66-67 4.5035897704643116E-4 68-69 7.369510533487056E-4 70-71 4.0941725186039196E-5 72-73 1.842377633371764E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.188345037207839E-5 82-83 0.0010644848548370192 84-85 0.0057523123886385075 86-87 0.004032759930824861 88-89 0.0 90-91 0.0012896643433602348 92-93 6.141258777905879E-4 94-95 4.0941725186039196E-5 96-97 0.0 98-99 0.0 100-101 2.0470862593019598E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2442496.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.13967090712685 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1496622692348 38.871489799546104 2 14.789860375088859 15.718566261891553 3 5.282085259800042 8.420648172274795 4 2.3709556992302763 5.039672223698949 5 1.2746163863274504 3.3866347651135977 6 0.774722813050477 2.4701109197847537 7 0.4994554802836547 1.8578629898524075 8 0.337579906832426 1.4351108123146927 9 0.22878645341380524 1.0941873158176487 >10 1.161233697960546 10.925708722732077 >50 0.07691634923138811 2.822270362805053 >100 0.04856565865356463 4.767212216474198 >500 0.0037836513019609687 1.3269680680303924 >1k 0.0016215648436936094 1.2985321587189003 >5k 1.54434747018439E-4 0.5650252109448035 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7563 0.30964226758201446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6207 0.2541252882297453 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4301 0.1760903600251546 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 6.14125877790588E-5 14-15 0.0 0.0 0.0 4.09417251860392E-5 1.02354312965098E-4 16-17 0.0 4.09417251860392E-5 0.0 6.14125877790588E-5 1.228251755581176E-4 18-19 0.0 4.09417251860392E-5 0.0 1.02354312965098E-4 1.228251755581176E-4 20-21 0.0 4.09417251860392E-5 0.0 1.637669007441568E-4 1.228251755581176E-4 22-23 0.0 4.09417251860392E-5 0.0 2.865920763022744E-4 1.228251755581176E-4 24-25 0.0 4.09417251860392E-5 0.0 5.936550151975685E-4 1.228251755581176E-4 26-27 0.0 4.09417251860392E-5 0.0 0.0015148438318834505 1.637669007441568E-4 28-29 0.0 4.09417251860392E-5 0.0 0.004401235457499214 1.637669007441568E-4 30-31 0.0 4.09417251860392E-5 0.0 0.01377689052510219 1.637669007441568E-4 32-33 0.0 4.09417251860392E-5 0.0 0.03330609343884289 2.04708625930196E-4 34-35 0.0 8.18834503720784E-5 0.0 0.06112599570275652 2.865920763022744E-4 36-37 0.0 1.02354312965098E-4 0.0 0.11156620113195682 2.865920763022744E-4 38-39 0.0 1.228251755581176E-4 0.0 0.1871855675505712 2.865920763022744E-4 40-41 0.0 1.228251755581176E-4 0.0 0.27003114846452153 2.865920763022744E-4 42-43 0.0 1.228251755581176E-4 0.0 0.3574212608741222 2.865920763022744E-4 44-45 0.0 1.228251755581176E-4 0.0 0.46380833377004504 2.865920763022744E-4 46-47 0.0 1.228251755581176E-4 0.0 0.5889467168011739 2.865920763022744E-4 48-49 0.0 1.432960381511372E-4 0.0 0.723890643014359 3.07062938895294E-4 50-51 0.0 1.637669007441568E-4 0.0 0.8671457394403103 3.275338014883136E-4 52-53 0.0 1.637669007441568E-4 0.0 1.004853232103553 3.275338014883136E-4 54-55 0.0 2.04708625930196E-4 0.0 1.1594696572686303 3.275338014883136E-4 56-57 0.0 2.04708625930196E-4 0.0 1.3454883856513993 3.275338014883136E-4 58-59 0.0 2.456503511162352E-4 0.0 1.5449974124829682 3.275338014883136E-4 60-61 0.0 2.456503511162352E-4 0.0 1.763073511686406 3.684755266743528E-4 62-63 0.0 2.456503511162352E-4 0.0 1.983442347500262 3.684755266743528E-4 64-65 0.0 2.456503511162352E-4 0.0 2.212286120427628 3.684755266743528E-4 66-67 0.0 2.456503511162352E-4 0.0 2.473248676763442 3.684755266743528E-4 68-69 0.0 2.456503511162352E-4 0.0 2.7650608230269365 3.684755266743528E-4 70-71 0.0 2.456503511162352E-4 0.0 3.0756447502882294 3.684755266743528E-4 72-73 0.0 2.456503511162352E-4 0.0 3.393700542395975 4.09417251860392E-4 74-75 0.0 2.456503511162352E-4 0.0 3.7150521433811967 4.503589770464312E-4 76-77 0.0 2.456503511162352E-4 0.0 4.062606448485484 4.503589770464312E-4 78-79 0.0 2.456503511162352E-4 0.0 4.455053355256263 5.1177156482549E-4 80-81 0.0 2.456503511162352E-4 0.0 4.879066332145477 5.731841526045488E-4 82-83 0.0 2.456503511162352E-4 0.0 5.306907360339586 6.14125877790588E-4 84-85 0.0 2.456503511162352E-4 0.0 5.742343078555707 6.14125877790588E-4 86-87 0.0 2.456503511162352E-4 0.0 6.190040843465045 6.550676029766272E-4 88-89 0.0 2.456503511162352E-4 0.0 6.664289317157531 6.960093281626663E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6465 0.0 39.246635 1 GTATCAA 9855 0.0 33.267654 1 ATCAACG 12315 0.0 26.498337 3 TATCAAC 12365 0.0 26.354391 2 TCAACGC 12500 0.0 26.105093 4 CAACGCA 12640 0.0 25.815956 5 AACGCAG 13060 0.0 25.203949 6 GTACATG 9620 0.0 24.893454 1 TACATGG 9550 0.0 24.522602 2 ACATGGG 10070 0.0 22.688822 3 ACGCAGA 14875 0.0 22.064781 7 GAGTACT 7855 0.0 21.799051 12-13 CGCAGAG 15045 0.0 21.689177 8 GTACTTT 8125 0.0 20.928503 14-15 AGTACTT 8035 0.0 20.60134 12-13 GCAGAGT 15960 0.0 20.148111 9 AGAGTAC 13195 0.0 19.978468 10-11 GGACCGA 810 0.0 19.937578 6 GTAAGGT 1085 0.0 19.262005 4 GTATAGA 955 0.0 18.906446 1 >>END_MODULE