FastQCFastQC Report
Thu 26 May 2016
SRR938635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992549
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45620.22895296426838188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.18910450884771215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.12250639758419994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.050.193811
GTATCAA50750.045.3541261
ATCAACG64650.035.23593
TCAACGC65050.035.019234
GTGGTAT11550.034.5863531
TATCAAC66300.034.4307172
CAACGCA67400.033.8687935
AACGCAG68800.033.2228366
ACGCAGA80450.028.4175327
CGCAGAG80050.028.3206068
TGGTATC13500.027.8299052
TACATGG61900.025.507382
GTACATG62900.025.4792541
TAGGCCG1151.271738E-424.8125725
GCAGAGT87600.024.3599769
ACATGGG63600.023.3300613
CAGAGTA84300.020.8036210-11
GAGTACT54850.020.7519112-13
AGAGTAC82000.020.37283710-11
CATGGGG41750.020.0481624
Produced by FastQC (version 0.11.5)