FastQCFastQC Report
Thu 26 May 2016
SRR938635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992549
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46590.23382110050994984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.2022534953971019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.13304566161233677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.051.2946781
GTATCAA46850.042.808381
ATCAACG60100.033.037693
TCAACGC61850.032.179714
TATCAAC62150.031.887532
CAACGCA63350.031.342785
AACGCAG66400.029.9603336
GTGGTAT12700.026.569331
ACGCAGA76700.025.6893717
CGCAGAG78050.025.3058628
GTACATG60250.024.7684351
GTAAGGT8100.024.6304384
TACATGG59400.024.0827472
TGGTATC13650.022.6311532
GCAGAGT88700.021.999819
CCGATCG1302.9514063E-421.9133429
GTGTAGC11650.021.6210041
TGCACCG5750.021.4789645
ACATGGG62400.021.3909243
TAAGGTG10350.021.1118035
Produced by FastQC (version 0.11.5)