##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938635_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1992549 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77478596511303 31.0 31.0 34.0 30.0 34.0 2 32.00488921476963 31.0 31.0 34.0 30.0 34.0 3 32.06560691857515 33.0 31.0 34.0 30.0 34.0 4 35.73930528182745 37.0 35.0 37.0 33.0 37.0 5 35.628438748557755 37.0 35.0 37.0 33.0 37.0 6 35.63313223413828 37.0 35.0 37.0 33.0 37.0 7 35.568062818028565 37.0 35.0 37.0 33.0 37.0 8 35.546989810539166 37.0 35.0 37.0 33.0 37.0 9 37.13080180211377 39.0 37.0 39.0 33.0 39.0 10-11 37.02905951120901 39.0 37.0 39.0 33.0 39.0 12-13 36.914208885201816 39.0 37.0 39.0 33.0 39.0 14-15 37.96098715765585 40.0 37.0 41.0 33.0 41.0 16-17 37.86023856878802 40.0 37.0 41.0 33.0 41.0 18-19 38.09186574583612 40.0 37.0 41.0 33.0 41.0 20-21 38.054434294965894 40.0 37.0 41.0 33.0 41.0 22-23 37.94330954972751 40.0 37.0 41.0 33.0 41.0 24-25 37.81012963796624 40.0 37.0 41.0 32.0 41.0 26-27 37.6140546606382 40.0 37.0 41.0 32.0 41.0 28-29 37.49303379741226 39.0 36.5 41.0 32.0 41.0 30-31 37.245616795371156 39.0 36.0 41.0 31.0 41.0 32-33 37.05469752563174 39.0 36.0 40.5 31.0 41.0 34-35 36.96666807190187 39.0 36.0 41.0 30.5 41.0 36-37 36.921954240523064 39.0 36.0 41.0 30.0 41.0 38-39 36.75900768312348 39.0 35.0 41.0 30.0 41.0 40-41 36.589729286456695 39.0 35.0 40.5 30.0 41.0 42-43 36.380556262355405 39.0 35.0 40.0 30.0 41.0 44-45 36.088126816454704 38.5 35.0 40.0 29.5 41.0 46-47 35.72434554934408 38.0 35.0 40.0 28.5 41.0 48-49 35.376335287112134 38.0 34.0 40.0 27.0 41.0 50-51 34.987043229551695 38.0 34.0 40.0 26.0 41.0 52-53 34.47856614818507 38.0 33.0 40.0 24.5 41.0 54-55 34.1143979395237 37.0 33.0 40.0 24.0 41.0 56-57 33.7563106352717 37.0 32.5 40.0 23.0 41.0 58-59 33.2480064480221 36.5 32.0 39.0 22.5 40.5 60-61 32.82224050700886 36.0 31.0 39.0 21.5 40.0 62-63 32.31891486733827 35.5 31.0 39.0 19.5 40.0 64-65 31.756785404022686 35.0 30.0 38.0 18.0 40.0 66-67 31.224731989025113 34.5 29.5 38.0 15.5 40.0 68-69 30.701388773877078 34.0 29.0 37.0 12.0 39.5 70-71 30.08766007761917 34.0 28.5 36.5 8.0 39.0 72-73 29.55900557527067 34.0 28.0 36.0 7.0 39.0 74-75 28.9381638795332 33.0 27.0 35.5 4.5 38.0 76-77 27.752286894826675 31.5 26.0 34.5 2.0 36.0 78-79 28.425600825876806 33.0 27.0 35.0 2.0 37.0 80-81 28.02269555228002 33.0 26.0 35.0 2.0 36.5 82-83 27.550916941063935 33.0 26.0 35.0 2.0 36.0 84-85 26.963217466672088 32.0 25.0 35.0 2.0 36.0 86-87 26.30718065151723 32.0 24.5 34.0 2.0 35.0 88-89 25.51870869925909 31.0 21.5 34.0 2.0 35.0 90-91 24.859378615030295 31.0 19.0 34.0 2.0 35.0 92-93 23.981069223391746 31.0 14.0 34.0 2.0 35.0 94-95 23.115839058412114 30.0 4.5 34.0 2.0 35.0 96-97 21.989621334280862 29.5 2.0 34.0 2.0 35.0 98-99 20.56712507446492 29.0 2.0 34.0 2.0 35.0 100-101 16.694303628166736 20.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22127377950124227 1101 2 0.3156718534488583 1101 3 0.35285079747728787 1101 4 0.2124433698647934 1101 5 0.14431372782885177 1101 6 0.14677939259951955 1101 7 0.2744277793318801 1101 8 0.21623685472982856 1101 9 0.2927312315752104 1101 10-11 0.25036804851865924 1101 12-13 0.3430109464627762 1101 14-15 0.41780394747767247 1101 16-17 0.41877684638392054 1101 18-19 0.39369360387110675 1101 20-21 0.388292609411117 1101 22-23 0.2946673568625684 1101 24-25 0.3938041240130872 1101 26-27 0.38716134108356215 1101 28-29 0.20702860591632088 1101 30-31 0.35646467353991085 1101 32-33 0.32960844047490667 1101 34-35 0.32579202093191384 1101 36-37 0.38357120067666983 1101 38-39 0.4082436672108045 1101 40-41 0.40364644256120386 1101 42-43 0.4961139976628246 1101 44-45 0.5669922763784854 1101 46-47 0.3646467348544178 1101 48-49 0.5518322317826758 1101 50-51 0.6967971217662594 1101 52-53 0.5680121603421213 1101 54-55 0.7258467284307741 1101 56-57 0.49351592064547134 1101 58-59 0.685989751982305 1101 60-61 0.6771938464373406 1101 62-63 0.7802591382129833 1101 64-65 0.5395563078159995 1101 66-67 0.2682433304405727 1101 68-69 0.6199203072427331 1101 70-71 0.5342780117434387 1101 72-73 0.39958863371303366 1101 74-75 0.4159324454469875 1101 76-77 0.36929678513346786 1101 78-79 0.6802455480110261 1101 80-81 0.9334670186161951 1101 82-83 0.8566325004431796 1101 84-85 0.9572952701580419 1101 86-87 0.748520720477778 1101 88-89 1.044870233284236 1101 90-91 0.999084697586607 1101 92-93 1.4293757046870859 1101 94-95 1.286433617683116 1101 96-97 1.367750531113181 1101 98-99 1.3343811847090485 1101 100-101 1.4599590600698527 1102 1 0.21484210667554748 1102 2 0.19855972956683487 1102 3 0.17608539621083708 1102 4 0.15491961587938619 1102 5 0.028657712159777304 1102 6 0.09314958079320235 1102 7 0.14646799154893841 1102 8 0.1322397197869094 1102 9 0.15437863939580154 1102 10-11 0.243806053627587 1102 12-13 0.164207931814218 1102 14-15 0.2953211092963457 1102 16-17 0.34295006824201835 1102 18-19 0.2677358064154234 1102 20-21 0.19633391187112892 1102 22-23 0.2495599489151985 1102 24-25 0.363859333409458 1102 26-27 0.25679097540846385 1102 28-29 0.30833917937144406 1102 30-31 0.3812046949644454 1102 32-33 0.30339468946865367 1102 34-35 0.26112895037670825 1102 36-37 0.3092934549441466 1102 38-39 0.3172085068753603 1102 40-41 0.24640006666825087 1102 42-43 0.11914145023408196 1102 44-45 0.2883176822087492 1102 46-47 0.4368227203516497 1102 48-49 0.5274206777427608 1102 50-51 0.5398183624880986 1102 52-53 0.5574120830109379 1102 54-55 0.4988643561381494 1102 56-57 0.7445086122144673 1102 58-59 0.5351050705297382 1102 60-61 0.44940913139008387 1102 62-63 0.5651713166071488 1102 64-65 0.5234589972932611 1102 66-67 0.2828500506474647 1102 68-69 0.28646544139966323 1102 70-71 0.5586071636992216 1102 72-73 0.524086393010208 1102 74-75 0.7103233989658726 1102 76-77 0.6488828731262188 1102 78-79 0.8155573765132722 1102 80-81 0.7782311268037923 1102 82-83 0.8831761133308618 1102 84-85 0.7534899419125125 1102 86-87 0.5806575332919337 1102 88-89 0.7903798901746342 1102 90-91 0.9223304522946201 1102 92-93 1.0850127044905875 1102 94-95 1.0520356916801887 1102 96-97 0.8773333864087824 1102 98-99 1.0657564754933269 1102 100-101 1.5187628353530336 1103 1 0.32752370924714214 1103 2 0.3490980679080913 1103 3 0.23530076094515806 1103 4 0.20586184076086056 1103 5 0.14870482772705884 1103 6 0.17765497726976065 1103 7 0.18719953780764342 1103 8 0.15675355022669635 1103 9 0.2606846203208093 1103 10-11 0.2504033941376136 1103 12-13 0.29754249211545414 1103 14-15 0.4675615864402758 1103 16-17 0.3780728066169772 1103 18-19 0.5165809537615118 1103 20-21 0.45377767495038057 1103 22-23 0.49792040276815186 1103 24-25 0.3954503805623304 1103 26-27 0.3690831186059924 1103 28-29 0.3881403068149467 1103 30-31 0.29112186474971935 1103 32-33 0.4335878825636712 1103 34-35 0.48218364539408043 1103 36-37 0.46622137272115793 1103 38-39 0.5380939791390134 1103 40-41 0.6202878593834669 1103 42-43 0.5649497779204893 1103 44-45 0.42386350217204694 1103 46-47 0.5811117982704488 1103 48-49 0.6018166822082591 1103 50-51 0.8227265679141524 1103 52-53 0.5541593005318433 1103 54-55 0.4922332945976464 1103 56-57 0.503743419037427 1103 58-59 0.5343669134668119 1103 60-61 0.727086747962943 1103 62-63 0.8130980908336127 1103 64-65 0.6304965887075156 1103 66-67 0.6003916071855251 1103 68-69 0.6835771424984252 1103 70-71 0.6268563060363093 1103 72-73 0.38356283016223713 1103 74-75 0.6232413699035284 1103 76-77 0.6492074843647337 1103 78-79 0.8081623421758728 1103 80-81 0.5983475866440209 1103 82-83 0.772818420896801 1103 84-85 0.891143758602027 1103 86-87 0.8925628604489262 1103 88-89 0.8038670671660704 1103 90-91 0.9531935945659917 1103 92-93 1.4457224914206144 1103 94-95 1.1297349984103882 1103 96-97 1.4154054179884668 1103 98-99 1.6331194834981808 1103 100-101 1.7153839880984272 1104 1 0.2371428749010569 1104 2 0.3066814243040632 1104 3 0.23446858074373012 1104 4 0.11675334207843946 1104 5 0.17463790195667173 1104 6 0.1212715197992722 1104 7 0.1850668654695724 1104 8 0.25376078186783246 1104 9 0.3333795798308401 1104 10-11 0.297289937990719 1104 12-13 0.31252931633001424 1104 14-15 0.48214170604513384 1104 16-17 0.48062926997144473 1104 18-19 0.3869013840501694 1104 20-21 0.4107069348201904 1104 22-23 0.34256176871807753 1104 24-25 0.3861659640871835 1104 26-27 0.35716751582884854 1104 28-29 0.525194706564676 1104 30-31 0.4678601659758428 1104 32-33 0.6945050134912663 1104 34-35 0.6685212583508715 1104 36-37 0.672308465264507 1104 38-39 0.5210531763185529 1104 40-41 0.6732234725087167 1104 42-43 0.8592168071719328 1104 44-45 0.794664397403487 1104 46-47 0.7514019062036041 1104 48-49 0.8055741274294874 1104 50-51 0.8922185481324192 1104 52-53 0.8625104622871689 1104 54-55 0.8772483956119927 1104 56-57 0.8743680354957277 1104 58-59 0.9901083070919015 1104 60-61 1.0800218797809649 1104 62-63 1.1341554024920768 1104 64-65 1.058802988890406 1104 66-67 1.1094566678904023 1104 68-69 1.1071663708426094 1104 70-71 1.1577790923238638 1104 72-73 1.1367760372326394 1104 74-75 1.31461104995455 1104 76-77 1.2149621139386575 1104 78-79 1.3714280117343982 1104 80-81 1.249221881228113 1104 82-83 1.4148047758368172 1104 84-85 1.5312100586730182 1104 86-87 1.45863804063832 1104 88-89 1.5135334009285693 1104 90-91 1.7307395293247971 1104 92-93 2.0179462511520434 1104 94-95 2.101268443246564 1104 96-97 1.9140425965654835 1104 98-99 1.9903398139155932 1104 100-101 2.238270267232558 1105 1 0.2198997636829212 1105 2 0.18286246481686774 1105 3 0.19714543007921748 1105 4 0.11830757511019385 1105 5 0.026206927378936484 1105 6 0.18902823657659695 1105 7 0.1972934069169341 1105 8 0.2018156398494213 1105 9 0.18356981685310103 1105 10-11 0.2686213678071283 1105 12-13 0.2677965540517562 1105 14-15 0.3708122256135127 1105 16-17 0.26624097545380465 1105 18-19 0.2630842592780809 1105 20-21 0.26746705728742626 1105 22-23 0.2426717369545699 1105 24-25 0.3027334414685896 1105 26-27 0.31900853776516414 1105 28-29 0.34200780643050877 1105 30-31 0.3889193699388258 1105 32-33 0.45575651853100396 1105 34-35 0.5150879330444127 1105 36-37 0.4737202785363763 1105 38-39 0.4090519608197951 1105 40-41 0.4818360838816531 1105 42-43 0.5196265219883998 1105 44-45 0.5818873267554991 1105 46-47 0.6639284235703258 1105 48-49 0.7093528378541052 1105 50-51 0.8117058599307896 1105 52-53 0.7945670632481026 1105 54-55 0.6252967174210298 1105 56-57 0.6853637124482717 1105 58-59 0.753260347639106 1105 60-61 0.7337035796445122 1105 62-63 0.6706592381933376 1105 64-65 0.8263186699710587 1105 66-67 0.7010505766362591 1105 68-69 0.7274293084508265 1105 70-71 0.8644264944819469 1105 72-73 1.056222985298735 1105 74-75 0.9880700612724489 1105 76-77 0.8476846018123361 1105 78-79 0.8883725230694957 1105 80-81 0.9129104612732917 1105 82-83 1.1071166315364742 1105 84-85 1.1802750419885797 1105 86-87 1.1690476407850952 1105 88-89 1.2552600971800167 1105 90-91 1.349187475017228 1105 92-93 1.5376946658435244 1105 94-95 1.6745798746683533 1105 96-97 1.8431281057562572 1105 98-99 1.8130660858891723 1105 100-101 1.8761776599877287 1106 1 0.06105097510391744 1106 2 0.16187414025442948 1106 3 0.11975234731447415 1106 4 0.08321510553553679 1106 5 0.05595978712909755 1106 6 -3.5842783482564755E-4 1106 7 0.16110309701177528 1106 8 0.20522419650198032 1106 9 -0.019295798760467164 1106 10-11 0.06284166700897487 1106 12-13 0.1215195036206751 1106 14-15 0.0656601284633993 1106 16-17 0.09863606338561226 1106 18-19 0.10837163927497073 1106 20-21 0.24994503455128125 1106 22-23 0.30676967549733547 1106 24-25 0.20029532201519373 1106 26-27 0.18872599108478028 1106 28-29 0.17139912228387288 1106 30-31 0.24580091230787815 1106 32-33 0.19213604618457225 1106 34-35 0.11548144139563732 1106 36-37 0.21274437600659724 1106 38-39 -0.11904754548169905 1106 40-41 0.09901627337676899 1106 42-43 0.25165913391627726 1106 44-45 0.093956750094911 1106 46-47 -0.5363534365335809 1106 48-49 -0.30672100867467833 1106 50-51 0.19378032574245907 1106 52-53 0.2830386030618044 1106 54-55 0.44747295569125356 1106 56-57 0.39261943899622764 1106 58-59 0.11936952330174222 1106 60-61 0.23604726073109816 1106 62-63 0.10334632573593439 1106 64-65 0.2270539206260338 1106 66-67 0.15046978295385216 1106 68-69 0.04086783803136029 1106 70-71 -0.011892323442516783 1106 72-73 0.30286251173374623 1106 74-75 0.36345711307668793 1106 76-77 0.24470358678858872 1106 78-79 0.4744746692802764 1106 80-81 -0.058830437496236954 1106 82-83 0.5138926184560582 1106 84-85 0.6239158660985957 1106 86-87 0.8902316623772464 1106 88-89 0.7958768929321565 1106 90-91 0.7688364298295873 1106 92-93 1.0937211249549392 1106 94-95 1.200258630537217 1106 96-97 1.3130476444496146 1106 98-99 1.4225372946911747 1106 100-101 1.186519547791125 1107 1 0.08770099911294693 1107 2 0.1383389839164515 1107 3 0.10815233686401626 1107 4 0.035357179537399475 1107 5 0.10070126887818276 1107 6 0.10687207417463895 1107 7 0.08350302710179847 1107 8 0.09285810428027474 1107 9 0.13500794385551274 1107 10-11 0.1643295962773763 1107 12-13 0.12105272392086874 1107 14-15 0.25370827084910985 1107 16-17 0.1019016068680827 1107 18-19 0.20813895888516498 1107 20-21 0.19269746045436875 1107 22-23 0.22237466703336395 1107 24-25 0.2859251288888913 1107 26-27 0.3537080090865956 1107 28-29 0.17130351408962952 1107 30-31 0.07939310473327765 1107 32-33 0.18392939464704483 1107 34-35 0.2596801073403796 1107 36-37 0.36423730527950227 1107 38-39 0.23647923877665988 1107 40-41 0.30222343842826405 1107 42-43 0.26505081264752306 1107 44-45 0.3215067298696681 1107 46-47 0.3002925874718514 1107 48-49 0.4512695390645476 1107 50-51 0.39742183803210906 1107 52-53 0.34550386203951433 1107 54-55 0.19206146432954796 1107 56-57 0.341082793467713 1107 58-59 0.24564013255454142 1107 60-61 0.18614068423868702 1107 62-63 0.4167026891200436 1107 64-65 0.41997785569264323 1107 66-67 0.4110678780872732 1107 68-69 0.5068786182212541 1107 70-71 0.6816649455279951 1107 72-73 0.5572925832923694 1107 74-75 0.5670647289394068 1107 76-77 0.4361627682833493 1107 78-79 0.6118085767883024 1107 80-81 0.5737040287510311 1107 82-83 0.7944592000475694 1107 84-85 1.0767882335511807 1107 86-87 1.057676858273819 1107 88-89 0.9043494005650459 1107 90-91 1.0771035994396492 1107 92-93 1.504339062448576 1107 94-95 1.5014918974140343 1107 96-97 1.3728434190404037 1107 98-99 1.3599921932991919 1107 100-101 1.266669394772972 1108 1 0.1955121455502855 1108 2 0.1829769350852537 1108 3 0.13310885410298567 1108 4 0.15730241713441018 1108 5 0.04467207186358735 1108 6 0.19854970997248245 1108 7 0.2352777762743372 1108 8 0.16826767476285198 1108 9 0.14629816119057182 1108 10-11 0.12072578104479703 1108 12-13 0.20772078563601326 1108 14-15 0.2801374642635821 1108 16-17 0.28201845342849907 1108 18-19 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-0.9119702276060089 2316 80-81 -0.9315746924950545 2316 82-83 -0.9365173820998116 2316 84-85 -1.1435978963719506 2316 86-87 -1.196335525707184 2316 88-89 -1.3378721566730327 2316 90-91 -1.0844671283920455 2316 92-93 -1.6040132845660615 2316 94-95 -1.2648046443230854 2316 96-97 -1.3872934613153127 2316 98-99 -1.3504391769722197 2316 100-101 -1.7603918928425273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 26.0 5 64.0 6 144.0 7 372.0 8 908.0 9 1630.0 10 2575.0 11 4015.0 12 5665.0 13 8080.0 14 10662.0 15 13370.0 16 16646.0 17 20453.0 18 20459.0 19 18879.0 20 19834.0 21 21251.0 22 22721.0 23 25909.0 24 29821.0 25 34298.0 26 40815.0 27 48381.0 28 57265.0 29 67192.0 30 79739.0 31 94500.0 32 111751.0 33 136012.0 34 170202.0 35 218607.0 36 276689.0 37 286746.0 38 122687.0 39 4169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.2944795559836 19.02176067821006 15.0043236292991 23.67943613650724 2 16.33704754258135 20.07776892366263 38.77032910695788 24.814854426798146 3 19.670482382114567 23.276064980083298 29.517015641773426 27.53643699602871 4 13.784805292115776 16.683905891398403 35.568259550957094 33.963029265528725 5 14.393285901497768 36.56763555479682 33.97719455923038 15.061883984475028 6 33.554005447293896 35.5271062342758 16.5927663510408 14.32612196738951 7 29.693523220758937 31.685444122076795 19.87900924895699 18.742023408207274 8 26.90990284304175 35.12500821811659 18.947187747955006 19.01790119088665 9 26.069522004226748 16.710655547241245 19.479822077148416 37.740000371383594 10-11 25.600549848460442 26.137751192066045 26.85173614300075 21.40996281647277 12-13 26.225101616070674 23.127235515914542 27.346253467292396 23.30140940072239 14-15 22.789853599585257 24.897606031269497 25.289992868431344 27.022547500713912 16-17 22.921117623707122 28.003075457617356 26.06171793014877 23.01408898852676 18-19 22.918884303472588 26.54268477211853 28.048494666881467 22.489936257527418 20-21 23.253756871223743 25.889099841459355 28.333280637013193 22.523862650303705 22-23 23.07052423804885 25.715728948196503 28.169972231548634 23.04377458220601 24-25 22.97053093700774 25.909933766192378 28.274656113960393 22.844879182839488 26-27 22.741997207036864 26.25821985613605 28.039969736625835 22.959813200201253 28-29 22.71459024188884 26.463785681395297 28.046934891138104 22.77468918557776 30-31 22.90771494238567 26.103422481068538 27.966067031314424 23.02279554523137 32-33 22.562851357702748 26.1207499880178 27.976975099853778 23.33942355442567 34-35 23.238858695106597 26.206987244995272 27.761233659720645 22.792920400177483 36-37 23.329191037480708 25.897824428735905 27.744162236769483 23.028822297013907 38-39 22.782415664203135 26.172535397709325 27.821245677154423 23.22380326093312 40-41 23.16864280247425 25.961330472641496 27.885823264450888 22.98420346043337 42-43 23.07873755720149 26.003905610890342 27.74120484733388 23.176151984574293 44-45 23.014608470358063 26.20793862991917 27.772255324872898 23.00519757484987 46-47 23.27132231127064 26.425824408835112 27.18555478434909 23.117298495545153 48-49 23.110046478154363 26.371220983775057 27.247460413771506 23.271272124299074 50-51 23.159025534512885 26.547567336841922 27.17478231913712 23.118624809508074 52-53 23.437792495943636 26.4490358831828 26.997679856304664 23.1154917645689 54-55 23.133613932704833 26.424521102794174 27.183634998987465 23.25822996551353 56-57 23.102016562704357 26.623285048447993 27.16003470930953 23.11466367953812 58-59 23.17295639632802 26.28217967658556 27.12557923794349 23.41928468914293 60-61 22.886287865442707 26.531568357917422 27.304146598151412 23.27799717848846 62-63 22.93952234738167 26.571920812006944 27.354364637694978 23.13419220291641 64-65 22.68737426062234 26.455966622093186 27.254700293542466 23.601958823742002 66-67 22.66054442664354 26.549331261468534 27.246552914864825 23.5435713970231 68-69 22.984939248006473 26.2327338949241 27.317992488698412 23.464334368371016 70-71 22.80548463850539 26.325075393453705 27.071557157748348 23.797882810292556 72-73 22.913469860332132 26.349145328206564 26.949532219667425 23.787852591793875 74-75 22.776891498309823 26.655465745159123 26.837786092852863 23.72985666367819 76-77 22.86115767589122 26.436663293248152 27.02851371331031 23.67366531755032 78-79 22.861856926732866 26.56551414609951 26.99239096956965 23.580237957597973 80-81 22.898713185139744 26.643545983544776 26.875497455712477 23.582243375603003 82-83 22.921013629800402 26.604142358816997 26.869579056657955 23.605264954724642 84-85 22.891359737537446 26.514249516114784 26.853847590211466 23.740543156136308 86-87 22.98348217893294 26.549728383759142 26.755191024793547 23.711598412514373 88-89 22.831146214842168 26.658208016981593 26.56538388029504 23.945261887881202 90-91 22.769097930348984 26.630438465815175 26.723862043345832 23.87660156049001 92-93 22.775268343274575 26.28747543972555 26.809804833040335 24.127451383959542 94-95 22.541905029406752 26.21494401660262 26.942463369887516 24.30068758410311 96-97 22.673836259159682 26.063326379631928 26.90226409505233 24.36057326615606 98-99 22.52535399431018 26.152256281410818 26.628140328800377 24.694249395478625 100-101 22.647501052203076 25.924050137319714 26.91100030944935 24.517448501027857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 277.0 1 207.0 2 184.0 3 252.0 4 219.5 5 196.5 6 363.0 7 789.0 8 1244.0 9 1413.5 10 1252.5 11 1039.0 12 1019.0 13 1117.5 14 1279.5 15 1401.0 16 1527.5 17 1668.0 18 1835.0 19 2095.5 20 2401.0 21 2870.5 22 3520.5 23 4375.0 24 5488.5 25 6974.0 26 8826.0 27 11143.5 28 13467.0 29 16064.0 30 19060.0 31 22609.0 32 27597.0 33 30621.5 34 32106.0 35 34899.5 36 38671.5 37 42587.5 38 45737.5 39 48568.0 40 52070.0 41 57071.5 42 61119.0 43 64363.0 44 68002.0 45 72184.0 46 74808.5 47 76403.5 48 77414.5 49 76876.5 50 76393.0 51 74872.0 52 73692.5 53 73947.5 54 75186.0 55 72610.5 56 66509.0 57 61188.0 58 56438.0 59 49858.5 60 40810.0 61 33739.0 62 28544.5 63 22237.5 64 16657.0 65 12955.0 66 9748.0 67 7661.0 68 5904.0 69 4230.0 70 3244.0 71 2340.5 72 1752.0 73 1414.5 74 1109.0 75 847.5 76 593.0 77 381.0 78 225.0 79 141.5 80 86.5 81 42.0 82 22.5 83 13.5 84 5.5 85 2.5 86 3.0 87 2.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.018697156255631E-4 2 0.05058846733505675 3 0.0 4 0.0 5 0.04612182686598924 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006900708589851492 26-27 0.0020827593198460867 28-29 7.528045734383445E-5 30-31 0.0016310765757830796 32-33 5.269632014068412E-4 34-35 0.011693564374075618 36-37 0.024968018352371762 38-39 0.004140425153910895 40-41 0.0012044873175013511 42-43 0.0015557961184392455 44-45 0.008908187452353744 46-47 0.0 48-49 0.0 50-51 5.269632014068412E-4 52-53 0.0 54-55 0.0014303286895328547 56-57 0.0 58-59 0.0246919900087777 60-61 0.0 62-63 0.010112674769855095 64-65 0.01671226153033125 66-67 0.007076362990320439 68-69 0.017615627018457262 70-71 1.003739431251126E-4 72-73 2.509348578127815E-5 74-75 0.022634324174712894 76-77 0.05011169110521247 78-79 0.03209456831425476 80-81 0.026975497214874012 82-83 0.036987798041604 84-85 0.017113757302831697 86-87 0.010840385857512161 88-89 0.08469051451181375 90-91 0.027427179958937023 92-93 0.02564554246846627 94-95 0.0288324151626886 96-97 0.040350325136295266 98-99 0.05508020128990554 100-101 0.015081184954548169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1992549.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.98316593304859 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21084445608065 46.72106382249906 2 12.027484371673552 14.18838212903139 3 3.7267520574308843 6.594469049843281 4 1.6238038527725884 3.8310836836323663 5 0.8767888939791836 2.5857892410914163 6 0.5373756024108072 1.9017668595221149 7 0.36383055373140527 1.5021914545576698 8 0.2791235818634177 1.3170874035901468 9 0.1982470675304571 1.0523915711900316 >10 1.0441190751321157 11.10331763234558 >50 0.07235272604838507 2.9171863472508432 >100 0.03518987803056655 3.691663139088213 >500 0.00281041977972366 1.195585219766519 >1k 0.0012774635362380238 1.3980224465914006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4659 0.23382110050994984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4030 0.2022534953971019 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 0.13304566161233677 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.01869715625563E-5 0.0 9 0.0 0.0 0.0 5.01869715625563E-5 0.0 10-11 0.0 0.0 0.0 5.01869715625563E-5 0.0 12-13 0.0 0.0 0.0 5.01869715625563E-5 5.01869715625563E-5 14-15 0.0 0.0 0.0 5.01869715625563E-5 7.528045734383446E-5 16-17 0.0 0.0 0.0 5.01869715625563E-5 1.003739431251126E-4 18-19 0.0 0.0 0.0 5.01869715625563E-5 1.003739431251126E-4 20-21 0.0 0.0 0.0 1.003739431251126E-4 1.2546742890639075E-4 22-23 0.0 0.0 0.0 2.509348578127815E-4 1.5056091468766892E-4 24-25 0.0 0.0 0.0 3.513088009378941E-4 1.5056091468766892E-4 26-27 0.0 0.0 0.0 8.28085030782179E-4 1.5056091468766892E-4 28-29 0.0 0.0 0.0 0.002183133262971199 1.5056091468766892E-4 30-31 0.0 0.0 0.0 0.008305943793603068 1.5056091468766892E-4 32-33 0.0 0.0 0.0 0.01999950816767869 1.5056091468766892E-4 34-35 0.0 0.0 0.0 0.04055107302254549 1.5056091468766892E-4 36-37 0.0 0.0 0.0 0.0752302703722719 1.5056091468766892E-4 38-39 0.0 0.0 0.0 0.13046103257686512 3.0112182937533784E-4 40-41 0.0 0.0 0.0 0.19023371570786968 3.0112182937533784E-4 42-43 0.0 0.0 0.0 0.2515371014715322 3.0112182937533784E-4 44-45 0.0 0.0 0.0 0.32852391584849355 3.0112182937533784E-4 46-47 0.0 0.0 0.0 0.42372860090266284 3.0112182937533784E-4 48-49 0.0 0.0 0.0 0.5233748329401184 3.513088009378941E-4 50-51 0.0 0.0 0.0 0.6314524762000835 4.014957725004504E-4 52-53 0.0 0.0 0.0 0.7386518474577036 4.014957725004504E-4 54-55 0.0 0.0 0.0 0.8588747378358073 4.014957725004504E-4 56-57 0.0 0.0 0.0 1.0030619071350315 4.5168274406300675E-4 58-59 0.0 0.0 0.0 1.1621044200167725 4.5168274406300675E-4 60-61 0.0 0.0 0.0 1.336253211338843 4.5168274406300675E-4 62-63 0.0 0.0 0.0 1.5016945630948098 4.5168274406300675E-4 64-65 0.0 0.0 0.0 1.6771733091632879 4.5168274406300675E-4 66-67 0.0 0.0 0.0 1.8804054505058594 5.01869715625563E-4 68-69 0.0 0.0 0.0 2.1118928568381508 5.520566871881193E-4 70-71 0.0 0.0 0.0 2.3609958901888985 5.520566871881193E-4 72-73 0.0 0.0 0.0 2.6133861701769945 6.273371445319538E-4 74-75 0.0 0.0 0.0 2.874734824589006 6.524306303132319E-4 76-77 0.0 0.0 0.0 3.1630338827301108 6.524306303132319E-4 78-79 0.0 0.0 0.0 3.482122647924844 6.524306303132319E-4 80-81 0.0 0.0 0.0 3.8241468591236654 6.524306303132319E-4 82-83 0.0 0.0 0.0 4.1814028161917225 6.524306303132319E-4 84-85 0.0 0.0 0.0 4.5371782575986845 6.775241160945101E-4 86-87 0.0 0.0 0.0 4.902388849659406 7.026176018757882E-4 88-89 0.0 0.0 0.0 5.302404106498761 7.528045734383446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2900 0.0 51.294678 1 GTATCAA 4685 0.0 42.80838 1 ATCAACG 6010 0.0 33.03769 3 TCAACGC 6185 0.0 32.17971 4 TATCAAC 6215 0.0 31.88753 2 CAACGCA 6335 0.0 31.34278 5 AACGCAG 6640 0.0 29.960333 6 GTGGTAT 1270 0.0 26.56933 1 ACGCAGA 7670 0.0 25.689371 7 CGCAGAG 7805 0.0 25.305862 8 GTACATG 6025 0.0 24.768435 1 GTAAGGT 810 0.0 24.630438 4 TACATGG 5940 0.0 24.082747 2 TGGTATC 1365 0.0 22.631153 2 GCAGAGT 8870 0.0 21.99981 9 CCGATCG 130 2.9514063E-4 21.913342 9 GTGTAGC 1165 0.0 21.621004 1 TGCACCG 575 0.0 21.478964 5 ACATGGG 6240 0.0 21.390924 3 TAAGGTG 1035 0.0 21.111803 5 >>END_MODULE