FastQCFastQC Report
Thu 26 May 2016
SRR938634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995996
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.23306659933186238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38080.1907819454548005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.12625275802155916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.047.928641
GTATCAA48800.045.740451
ATCAACG62350.035.3615723
TATCAAC63700.034.8359382
TCAACGC63700.034.6121524
CAACGCA66100.033.427325
AACGCAG67000.033.1852346
ACGCAGA77650.028.2033487
CGCAGAG79600.027.4527558
GTGGTAT11800.026.2167431
GTACATG63300.025.8643861
TACATGG62800.025.271842
GCAGAGT88850.023.6857689
ACATGGG63700.023.4228783
TGGTATC12650.022.537762
AGAGTAC82300.020.57711810-11
GAGTACT53250.020.16031612-13
CAGAGTA83850.020.02678310-11
AGTACTT55450.020.00294112-13
GTGTAGC11050.019.3819581
Produced by FastQC (version 0.11.5)