FastQCFastQC Report
Thu 26 May 2016
SRR938634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995996
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45880.2298601800805212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.19934909689197775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.14193415217264965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.050.778391
GTATCAA47600.043.7555271
ATCAACG62500.033.30753
TCAACGC63100.032.8401454
CAACGCA64000.032.378335
TATCAAC64600.032.0936932
GTGGTAT12500.030.813481
AACGCAG67600.030.7730566
ACGACCG700.00836908427.1587585
ACGCAGA78900.026.1851757
CGCAGAG79600.025.835578
TGGTATC14050.025.3834512
GTACATG61100.025.2156121
TACATGG60100.025.0813272
ACATGGG60550.024.0190163
GCAGAGT89650.022.6744489
AGAGTAC83950.019.8577310-11
TTACGTA1456.124013E-419.6666874
CAGAGTA85400.019.54837610-11
CATGGGG39900.019.416134
Produced by FastQC (version 0.11.5)