##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938634_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1995996 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.768496530053167 31.0 31.0 34.0 30.0 34.0 2 32.00453257421358 31.0 31.0 34.0 30.0 34.0 3 32.068641420123086 33.0 31.0 34.0 30.0 34.0 4 35.7368596931056 37.0 35.0 37.0 33.0 37.0 5 35.60186643660609 37.0 35.0 37.0 33.0 37.0 6 35.62823171990325 37.0 35.0 37.0 33.0 37.0 7 35.56938791460504 37.0 35.0 37.0 33.0 37.0 8 35.55583778725008 37.0 35.0 37.0 33.0 37.0 9 37.13986801576757 39.0 37.0 39.0 33.0 39.0 10-11 37.04507599213626 39.0 37.0 39.0 33.0 39.0 12-13 36.93081048258614 39.0 37.0 39.0 33.0 39.0 14-15 37.98689877134022 40.0 37.0 41.0 33.0 41.0 16-17 37.901212477379715 40.0 37.0 41.0 33.0 41.0 18-19 37.99684443255397 40.0 37.0 41.0 33.0 41.0 20-21 38.04848406509832 40.0 37.0 41.0 33.0 41.0 22-23 37.94905400612025 40.0 37.0 41.0 33.0 41.0 24-25 37.847342880446654 40.0 37.0 41.0 32.0 41.0 26-27 37.65160075471093 40.0 37.0 41.0 32.0 41.0 28-29 37.52524804658927 39.5 36.5 41.0 32.0 41.0 30-31 37.29446326545745 39.0 36.0 41.0 31.0 41.0 32-33 37.090963609145504 39.0 36.0 41.0 31.0 41.0 34-35 36.93273583714597 39.0 36.0 40.5 30.0 41.0 36-37 36.942313511650326 39.0 36.0 41.0 30.5 41.0 38-39 36.82279673907162 39.0 35.0 41.0 30.0 41.0 40-41 36.64890786354281 39.0 35.0 40.5 30.0 41.0 42-43 36.44340093867923 39.0 35.0 40.0 30.0 41.0 44-45 36.183417952741394 39.0 35.0 40.0 30.0 41.0 46-47 35.83602647500295 38.0 35.0 40.0 28.5 41.0 48-49 35.48517356748209 38.0 34.0 40.0 27.5 41.0 50-51 35.11708515447927 38.0 34.0 40.0 26.5 41.0 52-53 34.60894636061395 38.0 33.0 40.0 25.5 41.0 54-55 34.273152601508215 37.5 33.0 40.0 24.0 41.0 56-57 33.92090189559498 37.0 33.0 40.0 24.0 41.0 58-59 33.45552045194479 36.5 32.0 39.0 23.0 41.0 60-61 33.06397207208832 36.0 31.5 39.0 22.0 40.0 62-63 32.58633985238448 35.5 31.0 39.0 21.0 40.0 64-65 32.06019551141385 35.0 31.0 38.5 19.5 40.0 66-67 31.54936507888793 35.0 30.0 38.0 18.0 40.0 68-69 30.98963725378207 34.0 29.0 37.5 14.5 39.5 70-71 30.390329940540965 34.0 29.0 37.0 11.5 39.0 72-73 29.928912933693255 34.0 28.5 36.0 8.0 39.0 74-75 29.394197934264398 34.0 28.0 36.0 7.0 38.5 76-77 28.154946202296998 31.5 26.5 34.5 7.0 36.5 78-79 28.84924869588917 33.0 28.0 35.0 2.0 37.0 80-81 28.556619602444094 33.0 27.0 35.0 2.0 37.0 82-83 28.064671221786014 33.0 26.5 35.0 2.0 36.0 84-85 27.523638323924494 33.0 26.0 35.0 2.0 36.0 86-87 26.930137886047866 32.0 25.0 34.5 2.0 35.0 88-89 26.26166861055834 32.0 24.5 34.0 2.0 35.0 90-91 25.68222631708681 31.5 22.5 34.0 2.0 35.0 92-93 24.904244547584263 31.0 19.5 34.0 2.0 35.0 94-95 24.09358410537897 31.0 14.0 34.0 2.0 35.0 96-97 23.05898759316151 30.5 2.0 34.0 2.0 35.0 98-99 21.76821822288221 30.0 2.0 34.0 2.0 35.0 100-101 18.11428980819601 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2506410024999006 1101 2 0.28606866428158284 1101 3 0.3976535667766612 1101 4 0.2415954174390933 1101 5 0.18670431138633603 1101 6 0.2342970796639534 1101 7 0.3352463278243576 1101 8 0.3222519978461378 1101 9 0.28285343960667575 1101 10-11 0.3474371467750643 1101 12-13 0.46605058130339216 1101 14-15 0.5696775161732646 1101 16-17 0.5896903574026808 1101 18-19 0.5718676211743627 1101 20-21 0.48122478833732885 1101 22-23 0.5803762198473734 1101 24-25 0.5403202237817837 1101 26-27 0.5300029630555869 1101 28-29 0.5611033704678405 1101 30-31 0.6043930536214361 1101 32-33 0.5253397600630265 1101 34-35 0.4891016349347126 1101 36-37 0.5404349060658831 1101 38-39 0.6793463785354845 1101 40-41 0.6076005144742638 1101 42-43 0.7728250483151058 1101 44-45 0.7601035433440018 1101 46-47 0.6233388557050219 1101 48-49 0.8277623898660238 1101 50-51 0.900727106567281 1101 52-53 0.7997770022131903 1101 54-55 0.7387617336086052 1101 56-57 0.7938208537112885 1101 58-59 0.7931075690372467 1101 60-61 0.9696281716336514 1101 62-63 0.9577513559455042 1101 64-65 0.9878067374938908 1101 66-67 0.816755558636455 1101 68-69 0.831457641017348 1101 70-71 0.7905343367637201 1101 72-73 0.8079890700715353 1101 74-75 0.8867487144954218 1101 76-77 1.0758101331148957 1101 78-79 0.8208695445892218 1101 80-81 0.9774020186136845 1101 82-83 0.904577842098174 1101 84-85 1.035840433201745 1101 86-87 1.124343882679586 1101 88-89 1.0502182770333164 1101 90-91 1.0187016735910568 1101 92-93 1.1702712244245355 1101 94-95 1.4848364625679586 1101 96-97 1.7841639858115705 1101 98-99 1.4094393478170133 1101 100-101 1.2466157024034175 1102 1 0.3152364603110378 1102 2 0.3492079145125153 1102 3 0.31785989503003975 1102 4 0.30510659507711324 1102 5 0.14187283210096524 1102 6 0.23502162677119998 1102 7 0.24927317366654478 1102 8 0.2599282441840245 1102 9 0.3235332538055644 1102 10-11 0.37520137528483133 1102 12-13 0.39670034661313025 1102 14-15 0.4474371209953105 1102 16-17 0.507927299549678 1102 18-19 0.435233076434848 1102 20-21 0.5541599052963093 1102 22-23 0.42147379185242073 1102 24-25 0.34154102976677336 1102 26-27 0.5366384630184982 1102 28-29 0.4784928059003235 1102 30-31 0.5499084830197489 1102 32-33 0.26291423446151185 1102 34-35 0.3918067971042092 1102 36-37 0.5502048598188409 1102 38-39 0.45077564678354776 1102 40-41 0.6421229485054241 1102 42-43 0.5121118114593415 1102 44-45 0.5765327252154862 1102 46-47 0.5331619564617753 1102 48-49 0.650709701166214 1102 50-51 0.5167582956273336 1102 52-53 0.4125243568032886 1102 54-55 0.4728229674930233 1102 56-57 0.4637058102638676 1102 58-59 0.6152000062084397 1102 60-61 0.4653588819641854 1102 62-63 0.4885735695639468 1102 64-65 0.4532761739019264 1102 66-67 0.37872643854963073 1102 68-69 0.39183919179204096 1102 70-71 0.4921459205749166 1102 72-73 0.3155018004556709 1102 74-75 0.5035519577837064 1102 76-77 0.4798538782816202 1102 78-79 0.5484781936289664 1102 80-81 0.5907176803142029 1102 82-83 0.6806935481048022 1102 84-85 0.5484864731402439 1102 86-87 0.2068744218828691 1102 88-89 0.30352878662467475 1102 90-91 0.3136756742393949 1102 92-93 0.6604961896960653 1102 94-95 0.6334887846245749 1102 96-97 0.4669134642494335 1102 98-99 0.09182960128655893 1102 100-101 -0.34248834886158974 1103 1 0.17676119025150783 1103 2 0.14799032227574926 1103 3 0.15261693442884905 1103 4 0.18581181180654482 1103 5 0.2139701105554579 1103 6 0.15464516738364154 1103 7 0.19612357420009374 1103 8 0.12001100376223661 1103 9 0.23521926327159548 1103 10-11 0.2288982838253304 1103 12-13 0.1596815509162326 1103 14-15 0.30914222119687906 1103 16-17 0.3470201673004638 1103 18-19 0.2038914192102439 1103 20-21 0.28063354892433523 1103 22-23 0.31788064269922245 1103 24-25 0.29505459286660596 1103 26-27 0.4349259992475041 1103 28-29 0.36417128320342584 1103 30-31 0.48890564916122514 1103 32-33 0.6250623884865618 1103 34-35 0.4596888659449121 1103 36-37 0.4319166789675961 1103 38-39 0.30408007955317373 1103 40-41 0.35093592623867664 1103 42-43 0.3195631486785899 1103 44-45 0.27343655501537967 1103 46-47 0.29598014571791254 1103 48-49 0.2441018273534965 1103 50-51 0.08098300102256673 1103 52-53 0.1302686996233149 1103 54-55 0.052575135035304754 1103 56-57 0.233515299599091 1103 58-59 0.4010346804386842 1103 60-61 0.4010931438841183 1103 62-63 0.13632730899275458 1103 64-65 -0.03565281104118867 1103 66-67 -0.06690389043085077 1103 68-69 -0.11542632067024172 1103 70-71 -0.15481133849132078 1103 72-73 -0.1384017590987483 1103 74-75 -0.029860300451961308 1103 76-77 -0.10796387709416067 1103 78-79 0.03777411738503034 1103 80-81 -0.07892358740980754 1103 82-83 0.09569514726381811 1103 84-85 -0.12449840055098704 1103 86-87 0.01406121605250732 1103 88-89 0.07945536911028483 1103 90-91 0.04679934005730857 1103 92-93 0.06140535498464672 1103 94-95 -0.05721712631342868 1103 96-97 0.1272519653043851 1103 98-99 -0.17045950187035785 1103 100-101 -0.31682321028020866 1104 1 0.20129705329004466 1104 2 0.23732272711617597 1104 3 0.18383794639852624 1104 4 0.1016561928915678 1104 5 0.11533525790991206 1104 6 0.16040795496461158 1104 7 0.18944105307907222 1104 8 0.18337538905176132 1104 9 0.29749310736362133 1104 10-11 0.2766377101743842 1104 12-13 0.23125521500325874 1104 14-15 0.34974714308522437 1104 16-17 0.30625100727997534 1104 18-19 0.37673957120278345 1104 20-21 0.3271076847032859 1104 22-23 0.4031846146342133 1104 24-25 0.28583623498515465 1104 26-27 0.47868297936812354 1104 28-29 0.5004718521243134 1104 30-31 0.49557897294684494 1104 32-33 0.29485176950588965 1104 34-35 0.3731590205915296 1104 36-37 0.460178776748414 1104 38-39 0.37938654790777093 1104 40-41 0.4289950256881525 1104 42-43 0.45532238871589925 1104 44-45 0.3748279804709682 1104 46-47 0.4422952989849378 1104 48-49 0.42392287348470603 1104 50-51 0.43376782279134574 1104 52-53 0.4728405373823108 1104 54-55 0.47629637693835747 1104 56-57 0.4359512795751712 1104 58-59 0.418655461161066 1104 60-61 0.20848123202087265 1104 62-63 0.05809608798078614 1104 64-65 0.0188847600739237 1104 66-67 0.1341879646716997 1104 68-69 -0.05788516259432441 1104 70-71 0.08803247761452582 1104 72-73 0.03098293916295347 1104 74-75 -0.1143798710677899 1104 76-77 0.016276357306505673 1104 78-79 0.041817003246098494 1104 80-81 0.1253715682222456 1104 82-83 0.047376288784530374 1104 84-85 0.03139512058317351 1104 86-87 0.30478110463335994 1104 88-89 0.38524969041262835 1104 90-91 0.09170467319793474 1104 92-93 0.13564099071760793 1104 94-95 0.31308730548833097 1104 96-97 0.07975561139091525 1104 98-99 -2.982857198361444E-4 1104 100-101 -0.1937221315641544 1105 1 0.21902518309353525 1105 2 0.23900948121398002 1105 3 0.2249169538387008 1105 4 0.1889552600366997 1105 5 0.17045181559207379 1105 6 0.18838610748296247 1105 7 0.15604247891668876 1105 8 0.16272781410911108 1105 9 0.03978199731410115 1105 10-11 0.19527155410033004 1105 12-13 0.29007452928608757 1105 14-15 0.38401298002717965 1105 16-17 0.2583914965908676 1105 18-19 0.2974059268866043 1105 20-21 0.32405159913429316 1105 22-23 0.35795511023034265 1105 24-25 0.4118559449280781 1105 26-27 0.2645115753648781 1105 28-29 0.3549463988340378 1105 30-31 0.3481259177745528 1105 32-33 0.4285749788488644 1105 34-35 0.6632891556723592 1105 36-37 0.6346347675238349 1105 38-39 0.523330859497598 1105 40-41 0.39552057920483463 1105 42-43 0.4441915211393237 1105 44-45 0.4297040416296909 1105 46-47 0.30575158294599447 1105 48-49 0.29869912039009705 1105 50-51 0.2723981681602865 1105 52-53 0.3041275427018064 1105 54-55 0.36113132653947133 1105 56-57 0.342221799057036 1105 58-59 0.23267296612320365 1105 60-61 0.17297064408531782 1105 62-63 0.27975683941650686 1105 64-65 0.32193028440318017 1105 66-67 0.11885508188881388 1105 68-69 0.24284239207824143 1105 70-71 0.22749552170571263 1105 72-73 0.32146199325055136 1105 74-75 0.5268784666914286 1105 76-77 0.32733671514285234 1105 78-79 0.5234429049324341 1105 80-81 0.4691833608416509 1105 82-83 0.36549792429203265 1105 84-85 0.5069627111124397 1105 86-87 0.5534385860657132 1105 88-89 0.45489931016116714 1105 90-91 0.5455005373134192 1105 92-93 0.41938860097367225 1105 94-95 0.44418121106756914 1105 96-97 0.3671538745704517 1105 98-99 0.19065642225134738 1105 100-101 -0.2865754506971889 1106 1 0.18687813308779155 1106 2 0.19543202652792147 1106 3 0.18650856192203236 1106 4 0.06482677177157825 1106 5 -0.008410645029371722 1106 6 0.003980061701568616 1106 7 0.06945705839155636 1106 8 0.0983641332544849 1106 9 -0.08473128702311072 1106 10-11 0.03167006366933833 1106 12-13 -0.029003136944410812 1106 14-15 0.20650442718729778 1106 16-17 -0.0850324599533181 1106 18-19 0.24701842229846704 1106 20-21 0.17942886739972863 1106 22-23 0.3307076723464206 1106 24-25 0.31625186646330405 1106 26-27 0.3026435118511088 1106 28-29 0.36666913645260735 1106 30-31 0.4351744711934913 1106 32-33 0.3352415007500156 1106 34-35 0.3269657140264073 1106 36-37 -0.02518939374681395 1106 38-39 0.1291880721357188 1106 40-41 0.4227838945965843 1106 42-43 0.501495665075474 1106 44-45 0.4944681625714651 1106 46-47 0.2493394981078012 1106 48-49 0.3118534021336714 1106 50-51 0.2737295438902976 1106 52-53 0.4009858275386975 1106 54-55 -0.02869114424358088 1106 56-57 0.28063341317382395 1106 58-59 0.2530790660749176 1106 60-61 -0.20838281072028764 1106 62-63 -0.11022020357511764 1106 64-65 -0.3632175678836944 1106 66-67 -0.08752508448878871 1106 68-69 -0.8402061968315984 1106 70-71 -0.8255284664067055 1106 72-73 -0.5924651028373233 1106 74-75 -0.24065193640113236 1106 76-77 -0.2211763095504118 1106 78-79 -0.33300422716936495 1106 80-81 -0.7970837296610789 1106 82-83 -0.3985756184562952 1106 84-85 -0.19258452307239793 1106 86-87 0.01893722853879609 1106 88-89 0.20027521949872806 1106 90-91 0.15331605374803914 1106 92-93 -0.057515112215025255 1106 94-95 0.06191771822131997 1106 96-97 -0.19682594418312505 1106 98-99 -0.33538689345795447 1106 100-101 -0.5434320824053174 1107 1 0.14593907492234948 1107 2 0.14611008095888778 1107 3 0.1818967904692741 1107 4 0.13894304803637425 1107 5 0.12464595925947464 1107 6 0.20345300210696848 1107 7 0.14668130222261055 1107 8 0.2255029233386665 1107 9 0.3060524524146473 1107 10-11 0.24789102817018716 1107 12-13 0.33496041492749384 1107 14-15 0.28944121417193003 1107 16-17 0.3119709695752064 1107 18-19 0.3172422860822408 1107 20-21 0.37602926474259135 1107 22-23 0.3446983054323809 1107 24-25 0.3296596845139561 1107 26-27 0.35467141688025094 1107 28-29 0.31905125921016264 1107 30-31 0.38800013417119317 1107 32-33 0.4110609350484893 1107 34-35 0.2981069254897264 1107 36-37 0.35047915950190855 1107 38-39 0.3795711916640059 1107 40-41 0.34856543795269346 1107 42-43 0.4709052718516986 1107 44-45 0.5582936912809657 1107 46-47 0.4077529941104743 1107 48-49 0.49023349038656505 1107 50-51 0.4995264484392621 1107 52-53 0.6766552910803227 1107 54-55 0.4806691701715877 1107 56-57 0.5669985908288595 1107 58-59 0.411915279347447 1107 60-61 0.22837106200711332 1107 62-63 0.3062365267892986 1107 64-65 0.4107291663267105 1107 66-67 0.5703958292632301 1107 68-69 0.4524227063337172 1107 70-71 0.3971588775634025 1107 72-73 0.44388186895952586 1107 74-75 0.42635199378850075 1107 76-77 0.3395052950228994 1107 78-79 0.5418402834230314 1107 80-81 0.5270268572730323 1107 82-83 0.44734410736348096 1107 84-85 0.4847511178534596 1107 86-87 0.5655556041666152 1107 88-89 0.4794617134828272 1107 90-91 0.4320890471779997 1107 92-93 0.40898872580745405 1107 94-95 0.06552168163664973 1107 96-97 0.1881354480188513 1107 98-99 0.0991532607001524 1107 100-101 -0.351153186863705 1108 1 0.03776458941280225 1108 2 0.08562656583304218 1108 3 0.045380323244870624 1108 4 0.08994599554599603 1108 5 0.007845154430498269 1108 6 0.02096067975178073 1108 7 0.0928389498628377 1108 8 0.02369073441565206 1108 9 0.07055437623828453 1108 10-11 0.06018673221903725 1108 12-13 0.1514602913791805 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-0.2747135471056126 2316 72-73 -0.3616101082643368 2316 74-75 -0.21085206931046585 2316 76-77 -0.28301229018334695 2316 78-79 -0.1566924345156302 2316 80-81 -0.30085395319894914 2316 82-83 -0.0248146532559268 2316 84-85 0.02445166157660239 2316 86-87 -0.061370714227756196 2316 88-89 0.05114620464995312 2316 90-91 0.15968107039303803 2316 92-93 0.16565324466266773 2316 94-95 0.3680758263291075 2316 96-97 0.5404746948374068 2316 98-99 0.4514039102963885 2316 100-101 0.27808086155076595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 28.0 5 69.0 6 125.0 7 335.0 8 889.0 9 1521.0 10 2555.0 11 3824.0 12 5436.0 13 7599.0 14 9942.0 15 12874.0 16 15832.0 17 19180.0 18 19283.0 19 17611.0 20 17898.0 21 19126.0 22 20989.0 23 23271.0 24 27211.0 25 31427.0 26 38159.0 27 45247.0 28 54600.0 29 64410.0 30 76515.0 31 90640.0 32 108644.0 33 133578.0 34 168239.0 35 217442.0 36 282970.0 37 308579.0 38 144000.0 39 5935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.316091870521944 18.97841815286002 15.015358347240431 23.690131629377607 2 16.30006886865935 20.097468700848378 38.79546008174018 24.807002348752093 3 19.652561474845175 23.303551790875552 29.493446574337707 27.550440159941562 4 13.782342249182863 16.614612454133173 35.58684486341656 34.0162004332674 5 14.390936491962275 36.65212008526373 33.90887852927594 15.048064893498065 6 33.57677069493125 35.53799707013441 16.58204725861174 14.303184976322598 7 29.70136212697821 31.71990324629909 19.875190130641545 18.703544496081154 8 26.852007719454345 35.18954947805506 18.95414620069379 19.004296601796796 9 26.117938112100425 16.67403141088459 19.535660392104994 37.67237008490999 10-11 25.562851829362383 26.16207647710717 26.871146034360788 21.403925659169655 12-13 26.1672618582402 23.11542708502422 27.364959649217734 23.35235140751785 14-15 22.70235010491003 24.919714267964466 25.298397391577936 27.079538235547567 16-17 22.923242331146955 28.027761578680515 26.08286790153888 22.966128188633643 18-19 22.915627085425022 26.57119553345798 28.064810751123748 22.448366629993245 20-21 23.26783220006453 25.85689049477053 28.34417002839685 22.53110727676809 22-23 23.05189990360702 25.74125900051904 28.181519401842486 23.02532169403145 24-25 22.98851899206009 25.923701096998386 28.219677233175283 22.868102677766245 26-27 22.656082267507184 26.29228784708922 28.11473719837431 22.936892687029285 28-29 22.721313068763667 26.495343678043444 28.040311704031474 22.743031549161422 30-31 22.88150878057872 26.104135479229416 27.991814612854938 23.02254112733693 32-33 22.557685486343658 26.128384024817684 28.007095204599608 23.306835284239046 34-35 23.2039366896843 26.226125664117216 27.78535901975585 22.78457862644263 36-37 23.352257056691762 25.897478225777565 27.756675804128818 22.993588913401858 38-39 22.789318696789824 26.218643806670368 27.805808606818665 23.186228889721143 40-41 23.15525695371173 25.97206415469355 27.91304851032958 22.959630381265143 42-43 23.04919244233744 26.000087675899774 27.73018368101003 23.22053620075276 44-45 22.977757214382496 26.264788673120755 27.75201647498704 23.005437637509704 46-47 23.25425501854713 26.455714340108898 27.22269984508987 23.0673307962541 48-49 23.107987190355093 26.36746266024581 27.25135721714873 23.273192932250364 50-51 23.14363355437586 26.542012108240698 27.213957342599887 23.100396994783555 52-53 23.44363415557947 26.42324934518907 27.073851851406516 23.059264647824946 54-55 23.115527285625824 26.456666245824138 27.241612708642705 23.186193759907333 56-57 23.06981076114381 26.628184625620495 27.157845005701414 23.144159607534284 58-59 23.13882392549885 26.289356291295174 27.18299535670412 23.388824426501856 60-61 22.90220020481003 26.516435904681174 27.32037038150377 23.26099350900503 62-63 22.896138068412963 26.575353858424567 27.33655277866288 23.19195529449959 64-65 22.636919112062348 26.50852005715442 27.26799051701506 23.586570313768163 66-67 22.664967263186348 26.607337845870816 27.209719265258496 23.51797562568434 68-69 22.92498237699846 26.256794312141814 27.385994085084402 23.432229225775323 70-71 22.79954248359464 26.344843721975113 27.081394032246592 23.774219762183655 72-73 22.871814372373493 26.42197178751861 27.007393802392386 23.698820037715507 74-75 22.725847145986265 26.68447231357177 26.9123284816202 23.67735205882176 76-77 22.819659959238393 26.442988863705136 27.04917745326143 23.68817372379504 78-79 22.85267605746705 26.557768652842988 27.042564213555536 23.54699107613442 80-81 22.897789044925528 26.682490217788846 26.849777301262044 23.56994343602359 82-83 22.894799270232983 26.680534544568218 26.86513062884664 23.559535556352156 84-85 22.83586393212188 26.548152022813092 26.863778554881918 23.75220549018311 86-87 22.872990014987916 26.546569626414403 26.774757677016087 23.8056826815816 88-89 22.773259144752153 26.670088519782997 26.636020289635066 23.920632045829784 90-91 22.707366258752206 26.65666295076218 26.735398374187408 23.900572416298207 92-93 22.759469292747717 26.294778800167133 26.9300813936827 24.01567051340245 94-95 22.522771218672588 26.253716503869875 26.98525622928509 24.238256048172456 96-97 22.649318936510895 26.12209643706701 26.953260427375607 24.27532419904649 98-99 22.486420922713428 26.225686606694858 26.69587851864247 24.592013951949244 100-101 22.61735494459909 25.945492876739234 26.899527854765243 24.53762432389644 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 192.5 2 179.5 3 265.0 4 225.0 5 213.0 6 395.5 7 831.5 8 1329.5 9 1506.5 10 1300.0 11 1077.0 12 1086.0 13 1173.0 14 1312.0 15 1422.5 16 1539.0 17 1718.5 18 1895.5 19 2118.5 20 2424.0 21 2856.5 22 3537.0 23 4455.5 24 5505.5 25 6952.0 26 8835.5 27 11192.0 28 13398.5 29 15849.5 30 19150.5 31 22839.5 32 27750.5 33 30872.0 34 32168.5 35 34828.5 36 38720.0 37 42688.0 38 45897.0 39 48716.5 40 52390.0 41 57339.5 42 61318.5 43 64822.5 44 68233.0 45 72247.0 46 74942.5 47 76365.5 48 77576.0 49 77100.0 50 76586.0 51 75437.5 52 73843.0 53 74260.5 54 75839.5 55 72604.5 56 66405.5 57 61280.5 58 56515.5 59 49733.0 60 40578.5 61 33517.5 62 28319.5 63 21965.5 64 16244.5 65 12610.5 66 9611.5 67 7656.5 68 5907.0 69 4209.0 70 3220.0 71 2335.5 72 1717.0 73 1409.0 74 1100.5 75 824.5 76 567.0 77 332.5 78 198.0 79 127.0 80 73.5 81 34.5 82 20.5 83 13.0 84 6.5 85 4.0 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.513039104286782E-4 2 0.044789668917172176 3 8.517051136375023E-4 4 0.0 5 0.08582181527417891 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.0040120320882407E-4 26-27 0.008291599782765096 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01039581241645775 36-37 2.505015040110301E-5 38-39 0.003932873612973172 40-41 0.005085180531423911 42-43 4.2585255681875116E-4 44-45 2.505015040110301E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.515045120330903E-5 68-69 0.0012525075200551503 70-71 1.0020060160441204E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013026078208573564 82-83 8.266549632363992E-4 84-85 0.006237487449874649 86-87 0.002730466393720228 88-89 7.515045120330903E-5 90-91 0.003557121356956627 92-93 9.018054144397083E-4 94-95 2.505015040110301E-5 96-97 0.0 98-99 1.2525075200551502E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1995996.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.78489369050137 #Duplication Level Percentage of deduplicated Percentage of total 1 79.09865228363408 46.498058655553635 2 12.110020397668224 14.237725233334592 3 3.6580564541458354 6.451153793124459 4 1.6643939227727038 3.9136487923723977 5 0.8949950530545524 2.6306094523668246 6 0.5677408381724834 2.0024750889435348 7 0.35983533098892717 1.4807017174789319 8 0.25305463160778224 1.1900631693561974 9 0.20661962509915927 1.0931501426242793 >10 1.0759696576328608 11.343680398280691 >50 0.07141261202534692 2.8622886993856445 >100 0.0350701254020178 3.6718790671076613 >500 0.0028997613278086793 1.2349482232904518 >1k 0.001279306468150883 1.3896175667806658 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4588 0.2298601800805212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3979 0.19934909689197775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2833 0.14193415217264965 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.010030080220602E-5 9 0.0 0.0 0.0 5.010030080220602E-5 5.010030080220602E-5 10-11 0.0 0.0 0.0 5.010030080220602E-5 5.010030080220602E-5 12-13 0.0 0.0 0.0 5.010030080220602E-5 1.0020060160441204E-4 14-15 0.0 0.0 0.0 5.010030080220602E-5 1.0020060160441204E-4 16-17 0.0 0.0 0.0 7.515045120330902E-5 1.5030090240661804E-4 18-19 0.0 0.0 0.0 1.0020060160441204E-4 1.5030090240661804E-4 20-21 0.0 0.0 0.0 2.0040120320882407E-4 1.7535105280772105E-4 22-23 0.0 0.0 0.0 3.256519552143391E-4 2.0040120320882407E-4 24-25 0.0 0.0 0.0 5.260531584231632E-4 2.0040120320882407E-4 26-27 0.0 0.0 0.0 9.769558656430174E-4 2.0040120320882407E-4 28-29 0.0 0.0 0.0 0.0026803660929180222 2.0040120320882407E-4 30-31 0.0 0.0 0.0 0.007965947827550756 2.0040120320882407E-4 32-33 0.0 0.0 0.0 0.020541123328904468 2.0040120320882407E-4 34-35 0.0 0.0 0.0 0.04135779831222107 2.0040120320882407E-4 36-37 0.0 0.0 0.0 0.08103723654756823 2.0040120320882407E-4 38-39 0.0 0.0 0.0 0.13765057645406104 2.0040120320882407E-4 40-41 0.0 0.0 0.0 0.1975955863639005 2.0040120320882407E-4 42-43 0.0 0.0 0.0 0.26024601251705914 2.0040120320882407E-4 44-45 0.0 0.0 0.0 0.34048164425179206 2.0040120320882407E-4 46-47 0.0 0.0 0.0 0.437250375251253 2.0040120320882407E-4 48-49 0.0 0.0 0.0 0.5399309417453741 2.0040120320882407E-4 50-51 0.0 0.0 0.0 0.6531075212575577 2.0040120320882407E-4 52-53 0.0 0.0 0.0 0.7611237697871138 2.0040120320882407E-4 54-55 0.0 0.0 0.0 0.8827673001348699 2.0040120320882407E-4 56-57 0.0 0.0 0.0 1.0200421243329145 2.0040120320882407E-4 58-59 0.0 0.0 0.0 1.1798119835911494 2.0040120320882407E-4 60-61 0.0 0.0 0.0 1.3528083222611669 2.0040120320882407E-4 62-63 0.0 0.0 0.0 1.5203687782941449 2.505015040110301E-4 64-65 0.0 0.0 0.0 1.699853105918048 2.7555165441213306E-4 66-67 0.0 0.0 0.0 1.9155850011723472 3.006018048132361E-4 68-69 0.0 0.0 0.0 2.144919128094445 3.256519552143391E-4 70-71 0.0 0.0 0.0 2.396272337219113 3.507021056154421E-4 72-73 0.0 0.0 0.0 2.656543399886573 4.759528576209572E-4 74-75 0.0 0.0 0.0 2.917565967066066 5.010030080220602E-4 76-77 0.0 0.0 0.0 3.2028370798338273 5.010030080220602E-4 78-79 0.0 0.0 0.0 3.525933919707254 5.010030080220602E-4 80-81 0.0 0.0 0.0 3.875408567953042 5.010030080220602E-4 82-83 0.0 0.0 0.0 4.233099665530392 6.012036096264722E-4 84-85 0.0 0.0 0.0 4.59121661566456 6.012036096264722E-4 86-87 0.0 0.0 0.0 4.968321579802765 6.262537600275752E-4 88-89 0.0 0.0 0.0 5.375987727430315 6.513039104286782E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2800 0.0 50.77839 1 GTATCAA 4760 0.0 43.755527 1 ATCAACG 6250 0.0 33.3075 3 TCAACGC 6310 0.0 32.840145 4 CAACGCA 6400 0.0 32.37833 5 TATCAAC 6460 0.0 32.093693 2 GTGGTAT 1250 0.0 30.81348 1 AACGCAG 6760 0.0 30.773056 6 ACGACCG 70 0.008369084 27.158758 5 ACGCAGA 7890 0.0 26.185175 7 CGCAGAG 7960 0.0 25.83557 8 TGGTATC 1405 0.0 25.383451 2 GTACATG 6110 0.0 25.215612 1 TACATGG 6010 0.0 25.081327 2 ACATGGG 6055 0.0 24.019016 3 GCAGAGT 8965 0.0 22.674448 9 AGAGTAC 8395 0.0 19.85773 10-11 TTACGTA 145 6.124013E-4 19.666687 4 CAGAGTA 8540 0.0 19.548376 10-11 CATGGGG 3990 0.0 19.41613 4 >>END_MODULE