FastQCFastQC Report
Thu 26 May 2016
SRR938633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999333
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48650.2433311509388381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.19436482066769267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.1313438031583533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.049.524121
GTATCAA49550.044.2537461
ATCAACG64900.033.752783
TATCAAC65400.033.4220732
TCAACGC66650.032.7952544
GTGGTAT12400.032.6763231
CAACGCA68350.031.8405275
TGGTATC12700.031.0547262
AACGCAG70400.030.9094876
ACGCAGA82000.026.4743467
CGCAGAG82400.026.230538
TAAGGTG10000.024.7090855
GTACATG62350.023.7771531
GCAGAGT90150.023.3953369
TACATGG62050.023.2035352
GGACCGA6050.022.7741186
GTAAGGT9150.022.3306124
ACATGGG64800.021.0455463
GAGTACT53100.020.8425912-13
AGGACCG7300.020.8295755
Produced by FastQC (version 0.11.5)