##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938633_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1999333 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.017761923601523 31.0 31.0 34.0 28.0 34.0 2 31.164332804990465 31.0 31.0 34.0 27.0 34.0 3 31.22035948988988 31.0 31.0 34.0 27.0 34.0 4 34.88783859417116 37.0 35.0 37.0 32.0 37.0 5 34.88304849667364 37.0 35.0 37.0 32.0 37.0 6 34.87802832244554 37.0 35.0 37.0 32.0 37.0 7 34.78662133821629 37.0 35.0 37.0 32.0 37.0 8 34.741966945976486 37.0 35.0 37.0 31.0 37.0 9 36.215124744102155 39.0 35.0 39.0 32.0 39.0 10-11 36.067309197617405 39.0 35.0 39.0 30.0 39.0 12-13 35.8880469136457 38.0 35.0 39.0 30.0 39.0 14-15 36.85845329417361 39.0 36.0 41.0 30.5 41.0 16-17 36.6711680845562 39.0 36.0 41.0 30.0 41.0 18-19 36.513956404460885 39.0 36.0 40.0 30.0 41.0 20-21 36.48083885975973 39.0 36.0 40.5 29.5 41.0 22-23 36.57380911533997 39.0 36.0 41.0 30.0 41.0 24-25 36.47900974975154 39.0 36.0 41.0 29.5 41.0 26-27 36.277790143012695 39.0 35.5 41.0 29.0 41.0 28-29 36.07450759828403 39.0 35.0 40.0 28.0 41.0 30-31 35.79891368771486 38.5 35.0 40.0 27.0 41.0 32-33 35.49649258027552 38.0 34.0 40.0 27.0 41.0 34-35 35.21703888246731 38.0 34.0 40.0 25.5 41.0 36-37 34.917556755177856 38.0 34.0 40.0 25.0 41.0 38-39 34.58584487926724 38.0 33.0 40.0 24.5 41.0 40-41 34.462358946708726 38.0 33.0 40.0 24.0 41.0 42-43 34.22835015477662 38.0 33.0 40.0 23.5 41.0 44-45 33.92117221093235 38.0 33.0 40.0 22.5 41.0 46-47 33.53158853477635 38.0 32.5 40.0 20.0 41.0 48-49 33.13291507717824 37.0 31.5 40.0 18.0 41.0 50-51 32.34630524279848 36.5 30.5 39.5 15.0 40.5 52-53 32.34905040831117 36.5 31.0 39.0 14.5 40.0 54-55 32.38853907778244 37.0 31.0 39.5 12.5 41.0 56-57 32.02586912735397 36.0 30.5 39.0 8.5 41.0 58-59 31.569736257041722 36.0 30.0 39.0 7.5 41.0 60-61 31.045167563382385 35.0 29.0 39.0 4.0 40.0 62-63 30.415674127321463 35.0 28.0 38.5 2.0 40.0 64-65 29.89146980518003 34.5 28.0 38.0 2.0 40.0 66-67 29.30458082770604 34.0 26.5 38.0 2.0 40.0 68-69 28.662546459244158 34.0 26.0 37.0 2.0 39.5 70-71 28.00299875008315 33.5 25.5 36.5 2.0 39.0 72-73 27.343009893799582 33.0 24.5 36.0 2.0 39.0 74-75 26.618126395152785 32.5 22.5 35.5 2.0 38.0 76-77 25.85573763850244 32.0 20.5 35.0 2.0 37.0 78-79 25.04318440199807 31.0 17.0 35.0 2.0 37.0 80-81 24.265485039260593 31.0 9.0 34.5 2.0 36.0 82-83 23.521770010298432 30.5 4.5 34.0 2.0 36.0 84-85 22.77093085544029 30.0 2.0 34.0 2.0 35.0 86-87 22.0305444365696 29.0 2.0 34.0 2.0 35.0 88-89 21.239156258612248 29.0 2.0 34.0 2.0 35.0 90-91 20.419967309097583 27.5 2.0 34.0 2.0 35.0 92-93 19.542086035692904 26.0 2.0 33.5 2.0 35.0 94-95 18.633376731139833 25.0 2.0 33.0 2.0 35.0 96-97 17.568655146491352 22.0 2.0 33.0 2.0 35.0 98-99 16.326295069405646 9.5 2.0 32.0 2.0 34.0 100-101 13.647406660121149 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6037747209166966 1101 2 0.5385421873853602 1101 3 0.6405709532844206 1101 4 0.5356587867624327 1101 5 0.39408808098133363 1101 6 0.35479390857153703 1101 7 0.45281104999823185 1101 8 0.3272123152740605 1101 9 0.4538919238165704 1101 10-11 0.6649687209668471 1101 12-13 0.7522504777161885 1101 14-15 0.7988206876596138 1101 16-17 0.9435264701663684 1101 18-19 1.0028929330226433 1101 20-21 1.0978985868817688 1101 22-23 0.9686543262977239 1101 24-25 0.9680437675098972 1101 26-27 1.039449371541508 1101 28-29 1.0573088539900724 1101 30-31 0.8621081314137484 1101 32-33 0.9763691332037183 1101 34-35 1.1203440211111584 1101 36-37 1.3155444666787446 1101 38-39 1.382851530809269 1101 40-41 1.6244766782757551 1101 42-43 1.6962176985512016 1101 44-45 1.8616929229757062 1101 46-47 1.8385837479505582 1101 48-49 1.8490990327360564 1101 50-51 1.987414328163652 1101 52-53 2.0992073049194317 1101 54-55 2.1361082130658104 1101 56-57 2.2296262406525393 1101 58-59 2.5011471575259705 1101 60-61 2.544511875605405 1101 62-63 2.4937546111535873 1101 64-65 2.6679687025758447 1101 66-67 2.750293927149688 1101 68-69 2.813078323646817 1101 70-71 3.0562979385204727 1101 72-73 3.3699821762610966 1101 74-75 3.5301726148471744 1101 76-77 3.7185575620448645 1101 78-79 4.18669656463344 1101 80-81 4.532652350633306 1101 82-83 4.4836939308842325 1101 84-85 4.6828409000928986 1101 86-87 5.088859799851559 1101 88-89 5.296215448287352 1101 90-91 5.493597971173347 1101 92-93 5.5754055147187 1101 94-95 5.698450469578095 1101 96-97 5.609800486258983 1101 98-99 5.729756987275984 1101 100-101 5.613869673015211 1102 1 0.5045359131482456 1102 2 0.48301519536445525 1102 3 0.4797361813876151 1102 4 0.34763568457560723 1102 5 0.36938581687977745 1102 6 0.39481038194097096 1102 7 0.6268053019763897 1102 8 0.4921947720704196 1102 9 0.6114935632595859 1102 10-11 0.6700774389730881 1102 12-13 0.7043134660227963 1102 14-15 0.8885129129770775 1102 16-17 1.0189587403130105 1102 18-19 0.9387524772940523 1102 20-21 1.0065553892615782 1102 22-23 0.75866937149042 1102 24-25 0.8491488335384005 1102 26-27 0.9532803283887574 1102 28-29 0.9901559840299754 1102 30-31 0.9436777963167486 1102 32-33 0.8618880443410077 1102 34-35 1.0614173192957637 1102 36-37 1.2723657503551564 1102 38-39 1.2919875513999415 1102 40-41 1.5261143815182905 1102 42-43 1.7099657172805678 1102 44-45 1.5959219568219325 1102 46-47 1.7226731840128977 1102 48-49 1.8043950236622024 1102 50-51 1.8949951941269205 1102 52-53 1.9375536684179337 1102 54-55 2.009070464537089 1102 56-57 2.026737766194568 1102 58-59 2.240617989483056 1102 60-61 2.333746097628204 1102 62-63 2.6530326704120135 1102 64-65 2.5759425768090445 1102 66-67 2.55397909052272 1102 68-69 2.703154416903125 1102 70-71 2.7984224526807147 1102 72-73 2.939026211883462 1102 74-75 3.105600470629369 1102 76-77 3.1949914774510972 1102 78-79 3.5588082719434198 1102 80-81 4.0578672003475695 1102 82-83 4.379703449292652 1102 84-85 4.4402496318096425 1102 86-87 4.544117100171679 1102 88-89 4.812806837056794 1102 90-91 5.030365212642199 1102 92-93 4.9526818985959835 1102 94-95 5.3024740589030195 1102 96-97 5.328756598128329 1102 98-99 5.5151856376437784 1102 100-101 5.105512631948118 1103 1 0.5136921010859012 1103 2 0.5199997667860394 1103 3 0.459404623281106 1103 4 0.4575378869864011 1103 5 0.42759625549296487 1103 6 0.513222494239649 1103 7 0.5181799353882681 1103 8 0.5206711803015551 1103 9 0.580536844675251 1103 10-11 0.5466671519732316 1103 12-13 0.7608863757936462 1103 14-15 0.9009668656660352 1103 16-17 0.8626467871384804 1103 18-19 0.9853124440369285 1103 20-21 1.0034026003971235 1103 22-23 0.9621823293653264 1103 24-25 1.122685144757959 1103 26-27 1.0612410766040696 1103 28-29 1.1481779539902703 1103 30-31 1.1112507078342944 1103 32-33 1.2245932594555242 1103 34-35 1.4433527997860196 1103 36-37 1.4123845098855696 1103 38-39 1.5122469709025168 1103 40-41 1.4722462327206287 1103 42-43 1.5902077746106755 1103 44-45 1.6025061327912198 1103 46-47 1.8524528031979273 1103 48-49 1.7228731611237933 1103 50-51 1.9671006108103342 1103 52-53 2.0324596000277424 1103 54-55 2.3489873916072312 1103 56-57 2.4199464319098674 1103 58-59 2.706073554373745 1103 60-61 2.795214825630726 1103 62-63 2.966157878924026 1103 64-65 3.051170040482102 1103 66-67 2.9967826097686974 1103 68-69 3.2863120615864396 1103 70-71 3.410531607296626 1103 72-73 3.2027609304643008 1103 74-75 3.5161966359298233 1103 76-77 3.6035815607511026 1103 78-79 3.6295110905890624 1103 80-81 3.891299513202039 1103 82-83 4.220746122857609 1103 84-85 4.452579568980937 1103 86-87 4.398418953941665 1103 88-89 4.699617350079297 1103 90-91 4.982503082963888 1103 92-93 4.9825460579367125 1103 94-95 5.1705695822921705 1103 96-97 5.047381982183424 1103 98-99 5.145092765741328 1103 100-101 5.296634518241609 1104 1 0.640866577574652 1104 2 0.6601981905093268 1104 3 0.5301860144120525 1104 4 0.5670839467155773 1104 5 0.4919929201270463 1104 6 0.48949848338154567 1104 7 0.5769219208041605 1104 8 0.36735379495325304 1104 9 0.6026153025075089 1104 10-11 0.6846091643299204 1104 12-13 0.7768783801522829 1104 14-15 0.8460425344049725 1104 16-17 0.9889250013009132 1104 18-19 0.9341889289374592 1104 20-21 0.9811819817933056 1104 22-23 0.9472720458014905 1104 24-25 1.040504793012282 1104 26-27 1.1381828881075435 1104 28-29 1.045586466558028 1104 30-31 1.1751341744884556 1104 32-33 1.019502412468384 1104 34-35 1.1170830046202056 1104 36-37 1.4281424661380626 1104 38-39 1.5261582803812246 1104 40-41 1.608385617628734 1104 42-43 1.6403561293583309 1104 44-45 1.6797090534614227 1104 46-47 1.7347606426067728 1104 48-49 1.9293053948158985 1104 50-51 2.108705367783969 1104 52-53 1.9446050851002639 1104 54-55 2.2901299456662088 1104 56-57 2.531686482159248 1104 58-59 2.545271320212578 1104 60-61 2.6649104067399456 1104 62-63 2.784309115373947 1104 64-65 2.8920994736551187 1104 66-67 3.0428977840479483 1104 68-69 3.165011278499481 1104 70-71 3.3380234799580997 1104 72-73 3.4212370199125317 1104 74-75 3.4135145841481886 1104 76-77 3.7542980774994916 1104 78-79 4.046531054435491 1104 80-81 4.3700498454517245 1104 82-83 4.660085448111108 1104 84-85 4.831882307680537 1104 86-87 4.753139184670282 1104 88-89 5.213783634884329 1104 90-91 5.466594426671829 1104 92-93 5.60346076989277 1104 94-95 5.824344479971295 1104 96-97 5.9632098161078915 1104 98-99 6.083918772743527 1104 100-101 5.789944663252813 1105 1 0.5773506749682689 1105 2 0.4660831176041498 1105 3 0.4830295789764598 1105 4 0.36205322676598684 1105 5 0.2977518166594564 1105 6 0.43995096810909473 1105 7 0.4323333580776989 1105 8 0.31461350543304434 1105 9 0.613285890125006 1105 10-11 0.5886964054858979 1105 12-13 0.568073745028272 1105 14-15 0.8313039603007581 1105 16-17 0.8465962365754649 1105 18-19 0.8715587411169139 1105 20-21 0.8725103789296114 1105 22-23 0.7800522449998013 1105 24-25 0.776588043200455 1105 26-27 0.8472745928459489 1105 28-29 0.9391974172135633 1105 30-31 1.022677453733671 1105 32-33 1.0169100338165649 1105 34-35 1.1094494034719347 1105 36-37 1.2605218181309183 1105 38-39 1.31959768943792 1105 40-41 1.4081353069283935 1105 42-43 1.4830944934116133 1105 44-45 1.315851818081839 1105 46-47 1.5529300486877489 1105 48-49 1.5615202069078293 1105 50-51 1.5433725245694845 1105 52-53 1.7934226733654128 1105 54-55 1.8986422599024166 1105 56-57 2.1312593785866305 1105 58-59 2.217392309552494 1105 60-61 2.546562457362217 1105 62-63 2.6089883500883637 1105 64-65 2.832732077061447 1105 66-67 2.80783678175489 1105 68-69 2.845185970862378 1105 70-71 2.99027890189792 1105 72-73 3.249254314106377 1105 74-75 3.275523371279064 1105 76-77 3.420994501382282 1105 78-79 3.6584006828203712 1105 80-81 3.9883855418581042 1105 82-83 4.300905346936574 1105 84-85 4.697108375001861 1105 86-87 4.916987667927469 1105 88-89 5.22052451954886 1105 90-91 5.21628722717595 1105 92-93 4.968648327875581 1105 94-95 5.24063042931401 1105 96-97 5.7503739534111915 1105 98-99 6.1335643117867455 1105 100-101 5.811901987904042 1106 1 0.27245287835989274 1106 2 0.29691579590051376 1106 3 0.25013532602996946 1106 4 0.31098149228683525 1106 5 0.35143923419236245 1106 6 0.10087022878090579 1106 7 0.28485169617547257 1106 8 0.1940970425621913 1106 9 0.2102018703246742 1106 10-11 0.12300036848893825 1106 12-13 0.06253912324014976 1106 14-15 0.5852262511794706 1106 16-17 0.26157854763989263 1106 18-19 0.6599961342695124 1106 20-21 0.5373329549697061 1106 22-23 0.5119812730659277 1106 24-25 0.4828815679103329 1106 26-27 0.4535285215990683 1106 28-29 0.48436641675180425 1106 30-31 0.6534489169158917 1106 32-33 0.8541205985347986 1106 34-35 0.787780780506516 1106 36-37 0.7894497781636787 1106 38-39 0.7871823478258548 1106 40-41 0.8903439153478772 1106 42-43 0.515718976872364 1106 44-45 0.6303377109941692 1106 46-47 0.9769562172678263 1106 48-49 1.1048998686855143 1106 50-51 0.5078735156433609 1106 52-53 0.9309355538009001 1106 54-55 0.8147343152145581 1106 56-57 0.8582007260476168 1106 58-59 0.12940962367768094 1106 60-61 1.4865432116123252 1106 62-63 1.7127039077745074 1106 64-65 1.985010372014898 1106 66-67 1.870799640277479 1106 68-69 1.3214983065890955 1106 70-71 1.771122683511532 1106 72-73 2.4646336421970645 1106 74-75 2.791318985644992 1106 76-77 2.66176388489556 1106 78-79 2.8602584029157327 1106 80-81 3.248063580906532 1106 82-83 3.4845745409297386 1106 84-85 3.6756688681066443 1106 86-87 3.972970358502103 1106 88-89 3.8515957135490773 1106 90-91 4.292439064158106 1106 92-93 4.616022833954204 1106 94-95 4.621300435200531 1106 96-97 4.939262058997272 1106 98-99 4.942498663570387 1106 100-101 4.295433242273402 1107 1 0.5222042303262278 1107 2 0.19307702809373595 1107 3 0.35122199133870424 1107 4 0.39928411621173154 1107 5 0.318453457593904 1107 6 0.33555291351905936 1107 7 0.48831643678327197 1107 8 0.3828025497032499 1107 9 0.5084533516613945 1107 10-11 0.5695151212997658 1107 12-13 0.4726809581341911 1107 14-15 0.7035000236747777 1107 16-17 0.8831245420209939 1107 18-19 0.8225425608027379 1107 20-21 0.959966256923849 1107 22-23 0.8127149912073222 1107 24-25 0.9703412428878977 1107 26-27 0.9548878281004392 1107 28-29 1.1296374247580943 1107 30-31 1.156358374165869 1107 32-33 1.1312796617097476 1107 34-35 1.1431020319309653 1107 36-37 1.212496193686654 1107 38-39 1.2854125656268565 1107 40-41 1.36926617751282 1107 42-43 1.5091743037206697 1107 44-45 1.5967810073147248 1107 46-47 1.4851279981933132 1107 48-49 1.7620550599242932 1107 50-51 1.6889997306697708 1107 52-53 2.074599754172681 1107 54-55 2.0896009536215985 1107 56-57 2.148772446783763 1107 58-59 2.276500304055773 1107 60-61 2.283969498021829 1107 62-63 2.395911059112162 1107 64-65 2.6250073579603246 1107 66-67 2.85068059659066 1107 68-69 3.202227304087934 1107 70-71 3.034756578183856 1107 72-73 3.3213884045867985 1107 74-75 3.342085963901596 1107 76-77 3.7603456047692347 1107 78-79 3.9151031859863785 1107 80-81 4.348828311238105 1107 82-83 4.411394259177566 1107 84-85 4.572631439696174 1107 86-87 4.89580540025846 1107 88-89 4.881011074125425 1107 90-91 5.416686009273871 1107 92-93 5.657357472919095 1107 94-95 5.670684251424863 1107 96-97 5.454450692020302 1107 98-99 5.5347519010411865 1107 100-101 5.397860748985066 1108 1 0.41625493617468834 1108 2 0.5445914928926605 1108 3 0.45218836076903557 1108 4 0.4312387788871419 1108 5 0.40371215158101137 1108 6 0.5408805059047452 1108 7 0.5357091190074144 1108 8 0.43554970206673715 1108 9 0.5003812419861049 1108 10-11 0.6067899981539782 1108 12-13 0.5882364141425072 1108 14-15 0.6423808023918127 1108 16-17 0.9225918003495863 1108 18-19 0.9652401562099797 1108 20-21 0.903990260690243 1108 22-23 0.8098923878948625 1108 24-25 0.9364238893452992 1108 26-27 0.9255129027608646 1108 28-29 1.0232955045973213 1108 30-31 0.838210093288069 1108 32-33 0.9813896321266569 1108 34-35 1.1442087629637072 1108 36-37 1.1500677798605992 1108 38-39 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0.3636138126204358 2311 38-39 0.4378095029658269 2311 40-41 0.2757407970044099 2311 42-43 0.5428237312979007 2311 44-45 0.58406618276031 2311 46-47 0.650489447976824 2311 48-49 0.7689440550633506 2311 50-51 0.7269380126045917 2311 52-53 0.7990113938783736 2311 54-55 0.7433197094321571 2311 56-57 1.1703242968647807 2311 58-59 1.2087439091239034 2311 60-61 1.1173874994562034 2311 62-63 1.2764662826359476 2311 64-65 1.4308408241063617 2311 66-67 1.1952834201888258 2311 68-69 1.1334880951204198 2311 70-71 1.4278131298347212 2311 72-73 1.5074460585831346 2311 74-75 1.3579952661036288 2311 76-77 1.8571936271880212 2311 78-79 1.8061037969248304 2311 80-81 1.8598653792647752 2311 82-83 1.9490778727457148 2311 84-85 2.497599896678139 2311 86-87 2.7877718916123513 2311 88-89 2.583102542237093 2311 90-91 2.6484946527248745 2311 92-93 3.070993685631926 2311 94-95 3.037069023119816 2311 96-97 2.8648525832732545 2311 98-99 3.009697447831101 2311 100-101 2.978390074030882 2312 1 -0.36149488409849795 2312 2 -0.3589925228180917 2312 3 -0.25731085895357 2312 4 -0.3007753888461977 2312 5 -0.1861126805278417 2312 6 -0.19891364113809118 2312 7 -0.26731029148862717 2312 8 -0.21180349181027935 2312 9 -0.36995331290509625 2312 10-11 -0.3821063915288292 2312 12-13 -0.3676761597208298 2312 14-15 -0.2951886773648056 2312 16-17 -0.0024568573979522057 2312 18-19 0.058883087247487254 2312 20-21 -0.0840333580434276 2312 22-23 -0.23190286381035463 2312 24-25 -0.4007747394705987 2312 26-27 -0.1684171528229399 2312 28-29 -0.19362380244621846 2312 30-31 -0.11009389927738056 2312 32-33 0.09836636407946031 2312 34-35 -0.057299627210063875 2312 36-37 -0.02460783515579834 2312 38-39 0.16046200732940719 2312 40-41 0.07502329186754508 2312 42-43 0.05039205722971474 2312 44-45 0.14817325065540388 2312 46-47 0.11739417831861942 2312 48-49 0.38264293489365997 2312 50-51 0.47523787341190626 2312 52-53 0.6135956938275484 2312 54-55 0.7354741028392766 2312 56-57 0.7620495128343769 2312 58-59 0.8660912942899444 2312 60-61 0.9322512894740989 2312 62-63 1.1832386694590404 2312 64-65 1.3124049742577064 2312 66-67 1.289024168294258 2312 68-69 1.3747692201507995 2312 70-71 1.4274855851494515 2312 72-73 1.5162582810262997 2312 74-75 1.480757688498187 2312 76-77 1.6970982912475883 2312 78-79 1.9372023144122963 2312 80-81 2.042589468436468 2312 82-83 2.341227294985611 2312 84-85 2.3724927404067344 2312 86-87 2.898085406558991 2312 88-89 2.822150508477346 2312 90-91 3.0924403233905693 2312 92-93 3.2511300060844164 2312 94-95 3.312214844025231 2312 96-97 3.4598245237960015 2312 98-99 3.439501804783003 2312 100-101 3.136383710936828 2313 1 -0.3050840817824607 2313 2 -0.3543628647363306 2313 3 -0.3632188145116615 2313 4 -0.2338897464270886 2313 5 -0.25676956388758754 2313 6 -0.20539248470029037 2313 7 -0.16230728450452858 2313 8 -0.16449682771893492 2313 9 -0.3215687437817749 2313 10-11 -0.30092989253628133 2313 12-13 -0.186601612853579 2313 14-15 -0.25171877134952325 2313 16-17 -0.18721254542664667 2313 18-19 0.027641071119511196 2313 20-21 0.023096157371206516 2313 22-23 -0.1700967199053096 2313 24-25 0.03326015032453 2313 26-27 0.02136371046682939 2313 28-29 0.05066292775487824 2313 30-31 0.22375563299392098 2313 32-33 0.19246345674284981 2313 34-35 0.22712232948058642 2313 36-37 0.2196378508097112 2313 38-39 0.3140362578879987 2313 40-41 0.5799588063252159 2313 42-43 0.63382884252227 2313 44-45 0.7846795741015455 2313 46-47 0.6835371834590305 2313 48-49 0.6688297937146643 2313 50-51 0.6657085871426318 2313 52-53 0.7348675822574648 2313 54-55 0.8391101392391533 2313 56-57 1.058367274233479 2313 58-59 1.2470177965748093 2313 60-61 1.3236875304366897 2313 62-63 1.3977701945048047 2313 64-65 1.2729387468594702 2313 66-67 1.5173214251345968 2313 68-69 1.9139255306471519 2313 70-71 1.9728048961330629 2313 72-73 1.8990480160166214 2313 74-75 2.0701504971969307 2313 76-77 2.278246586046162 2313 78-79 2.514440322691378 2313 80-81 2.5130436547290103 2313 82-83 2.632343193371497 2313 84-85 2.777079269117948 2313 86-87 2.873887305568225 2313 88-89 2.9713388422569693 2313 90-91 3.194950496654311 2313 92-93 3.1589764786945196 2313 94-95 3.225624566460848 2313 96-97 3.3022665680184247 2313 98-99 3.060978585159031 2313 100-101 2.9404189958101608 2314 1 -0.3917592253959583 2314 2 -0.3310318264238923 2314 3 -0.28777202242024913 2314 4 -0.1954288210685462 2314 5 -0.2802881781229587 2314 6 -0.17204545985313757 2314 7 -0.40982605246917814 2314 8 -0.274432914334902 2314 9 -0.3137054038690934 2314 10-11 -0.2453443117311238 2314 12-13 -0.3314175406043134 2314 14-15 -0.33612193162169035 2314 16-17 -0.2986703116921987 2314 18-19 -0.4201897847485867 2314 20-21 -0.0970871647927396 2314 22-23 -0.2551875129846124 2314 24-25 -0.26342480008140257 2314 26-27 -0.20586181016554406 2314 28-29 -0.2678155498186712 2314 30-31 -0.38404352931252816 2314 32-33 -0.20583294334982583 2314 34-35 -0.21328956016886735 2314 36-37 -0.12456091066512442 2314 38-39 -0.021295119327618295 2314 40-41 -0.019587452102875602 2314 42-43 0.05358270535251819 2314 44-45 0.10952984637050633 2314 46-47 -0.018969586415884976 2314 48-49 0.1542413438058432 2314 50-51 0.18257764582813252 2314 52-53 0.2532108261068231 2314 54-55 0.3262964545383369 2314 56-57 0.3755499712171293 2314 58-59 0.6668851002571756 2314 60-61 0.7847070044860267 2314 62-63 0.7840115114755761 2314 64-65 0.8684364006318752 2314 66-67 0.9961162836763684 2314 68-69 1.1651626255219156 2314 70-71 1.304294133227497 2314 72-73 1.4358494731256073 2314 74-75 1.400879822519748 2314 76-77 1.7371890771024603 2314 78-79 1.8726092322987782 2314 80-81 2.019066938163654 2314 82-83 2.0175240185264975 2314 84-85 2.333039128307167 2314 86-87 2.7637375483578523 2314 88-89 2.657751641616894 2314 90-91 2.6639214210760613 2314 92-93 2.748896096188126 2314 94-95 2.860495039717737 2314 96-97 2.967002993275532 2314 98-99 3.17793785061259 2314 100-101 3.1322967181449517 2315 1 -0.5851180364024735 2315 2 -0.5972597920757217 2315 3 -0.6569752377318103 2315 4 -0.46370292224150944 2315 5 -0.5493471438675073 2315 6 -0.4980328856455998 2315 7 -0.49259242238313306 2315 8 -0.5667461759793682 2315 9 -0.6760231861741701 2315 10-11 -0.502539696541568 2315 12-13 -0.4826084127200687 2315 14-15 -0.5312948706773 2315 16-17 -0.41327636034937854 2315 18-19 -0.391510917387599 2315 20-21 -0.38843079673527825 2315 22-23 -0.5226161200701611 2315 24-25 -0.38079070872422704 2315 26-27 -0.5726534532940093 2315 28-29 -0.547508467959311 2315 30-31 -0.7192038246800578 2315 32-33 -0.5280247180487692 2315 34-35 -0.604448626129134 2315 36-37 -0.43956333031386663 2315 38-39 -0.2611370754393718 2315 40-41 -0.3019270477571112 2315 42-43 -0.1721928711755396 2315 44-45 -0.1972788482561043 2315 46-47 -0.12240851910627981 2315 48-49 -0.30547609609735815 2315 50-51 -0.08774192703914707 2315 52-53 0.11365307638236288 2315 54-55 0.014410701788598601 2315 56-57 0.3696848400261423 2315 58-59 0.5176515215070623 2315 60-61 0.5447017469776476 2315 62-63 0.647120243431381 2315 64-65 0.7345883359857659 2315 66-67 0.8263548563956071 2315 68-69 0.7298463529802923 2315 70-71 0.9129214256704294 2315 72-73 1.2876837704461117 2315 74-75 1.3129317311214166 2315 76-77 1.5264360120480944 2315 78-79 1.786328231309902 2315 80-81 1.9883201396344283 2315 82-83 1.90925309044572 2315 84-85 1.7962616844342776 2315 86-87 2.4303993469612024 2315 88-89 2.352471850784301 2315 90-91 2.451253105021035 2315 92-93 2.6355746630568433 2315 94-95 2.6189658571164607 2315 96-97 2.7519280837385303 2315 98-99 2.9972378850652 2315 100-101 2.8431403955596384 2316 1 -0.8035416688107837 2316 2 -0.682522371761813 2316 3 -0.6576898931595494 2316 4 -0.5311594396813746 2316 5 -0.4743310339586557 2316 6 -0.31280134296451223 2316 7 -0.4280468069751535 2316 8 -0.551509182897405 2316 9 -0.4112791894216983 2316 10-11 -0.47493261177742596 2316 12-13 -0.4742419054334732 2316 14-15 -0.9972910166004283 2316 16-17 -0.6526756381712531 2316 18-19 -0.613009389666729 2316 20-21 -0.5472730785986073 2316 22-23 -0.5784636857943326 2316 24-25 -0.6602083248740698 2316 26-27 -0.3281378008971103 2316 28-29 -0.4759044178194003 2316 30-31 -0.19083654619958423 2316 32-33 -0.32425305956558503 2316 34-35 -0.3304834216397481 2316 36-37 -0.19506778573271077 2316 38-39 -0.24902081536062326 2316 40-41 -0.30838142004787983 2316 42-43 -0.10682259875958522 2316 44-45 -0.24460140979311973 2316 46-47 -0.032783726216784714 2316 48-49 0.37050455024826334 2316 50-51 0.38008403011654224 2316 52-53 0.5944851236040307 2316 54-55 0.5102149103307738 2316 56-57 0.8441996029478993 2316 58-59 0.9350657652213599 2316 60-61 0.5538432331392151 2316 62-63 0.9717988968795659 2316 64-65 1.0149388365047862 2316 66-67 1.199730026602353 2316 68-69 1.4055420597487256 2316 70-71 1.2526281874793952 2316 72-73 1.4010764746681872 2316 74-75 1.799599776159134 2316 76-77 1.621094500905528 2316 78-79 1.7985245678078101 2316 80-81 2.205381408385559 2316 82-83 2.6508954542543 2316 84-85 2.7635863655716335 2316 86-87 2.783352309812372 2316 88-89 2.707705867909535 2316 90-91 2.8110492306944117 2316 92-93 2.8840623187504733 2316 94-95 3.268278926818194 2316 96-97 3.2367568550421737 2316 98-99 3.3401154128541997 2316 100-101 2.9219098066987055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6615.0 3 2586.0 4 2889.0 5 3304.0 6 4001.0 7 5052.0 8 6327.0 9 8302.0 10 10136.0 11 12295.0 12 14864.0 13 17517.0 14 19613.0 15 22385.0 16 25243.0 17 28227.0 18 28747.0 19 28744.0 20 30728.0 21 33619.0 22 36488.0 23 40621.0 24 45174.0 25 50212.0 26 55944.0 27 62064.0 28 68481.0 29 76720.0 30 84935.0 31 96212.0 32 109773.0 33 126286.0 34 148018.0 35 174699.0 36 200310.0 37 199570.0 38 104890.0 39 7742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74191981851229 18.0931850300732 14.350301986200794 23.81459316521372 2 15.84681791277173 19.276048074666736 39.87734665705347 24.99978735550807 3 19.575617681629197 23.019633411576095 29.028897447611463 28.37585145918325 4 13.258428315657897 15.924655306483752 35.989135536583 34.82778084127535 5 14.47837145033446 36.537019817370705 33.741578443216866 15.243030289077975 6 33.6403682617469 34.94532926555844 16.93426499791807 14.48003747477659 7 29.779748378207188 31.64234602849632 19.895092544506117 18.682813048790372 8 26.92917514513249 34.753996490703166 19.174349611820936 19.142478752343408 9 26.37547610517446 16.756050013309927 19.59416724876758 37.27430663274803 10-11 25.603317024064637 25.96611027694069 26.89998644090863 21.530586258086046 12-13 26.430418051574357 23.054153622308093 27.209026787863078 23.306401538254473 14-15 22.948652330685174 24.8129953415815 25.283264965500884 26.955087362232437 16-17 23.06512029876768 27.864038691715727 26.038489102008498 23.03235190750809 18-19 23.05349152284589 26.435998086836797 27.99709723511895 22.51341315519836 20-21 23.426877758116138 25.774899445018058 28.274257099017852 22.52396569784795 22-23 23.187913804981996 25.665527746604376 28.084903163442615 23.061655284971007 24-25 23.037671315997617 25.87909037157365 28.18398849590228 22.899249816526456 26-27 22.69137136866651 26.164341061996943 28.022097622854226 23.12218994648232 28-29 22.812956031581745 26.458932045805994 27.95340844243066 22.7747034801816 30-31 22.943334351568247 26.07101675634389 27.91777465385642 23.067874238231443 32-33 22.63046899039604 26.059625097878325 27.930408199514172 23.37949771221146 34-35 23.3170866919218 26.202381134049425 27.67706216560567 22.803470008423105 36-37 23.39192756053404 25.89736925120185 27.61461947941389 23.096083708850223 38-39 22.866485703365598 26.18625846201872 27.66619875013257 23.281057084483116 40-41 23.256527119132883 25.965276243985276 27.804143028197075 22.974053608684766 42-43 23.159696227405718 25.940834831843745 27.642420605629166 23.257048335121375 44-45 23.019423538995266 26.259006030823656 27.652700790062156 23.068869640118923 46-47 23.29924137258286 26.48535146235252 27.08064762219204 23.13475954287258 48-49 23.23608765892288 26.334869651410784 27.131825795686787 23.29721689397955 50-51 23.223986520537448 26.560363587343772 27.11403796388486 23.10161192823392 52-53 23.493778990798223 26.391164413761576 27.040513276713302 23.074543318726903 54-55 23.123110805877754 26.42048140280182 27.19979351730368 23.256614274016744 56-57 23.060410909227326 26.6742862859358 27.17960709775038 23.085695707086494 58-59 23.207829973096597 26.24896239200936 27.130019440378213 23.413188194515826 60-61 22.955537954066116 26.46456744311911 27.349846776172164 23.230047826642615 62-63 22.942120501778543 26.563206124701356 27.379112701597624 23.115560671922474 64-65 22.76916427961707 26.451719238448433 27.325413155704997 23.4537033262295 66-67 22.822262486667093 26.508659981768464 27.248925552041797 23.420151979522654 68-69 23.05180318750369 26.169978521215175 27.39267860554967 23.385539685731462 70-71 22.931476848718415 26.30015702482233 27.144634418300317 23.62373170815894 72-73 23.025723398678164 26.370635646381558 27.04821223829886 23.555428716641423 74-75 22.95322525983535 26.675270527649282 26.902147338618203 23.469356873897162 76-77 23.105156519375058 26.420799653048988 26.963485857219354 23.510557970356597 78-79 23.20176330399912 26.530542319020867 26.889210517299034 23.378483859680976 80-81 23.262694654029538 26.65032002993215 26.70819352657179 23.378791789466526 82-83 23.27881858363697 26.60025151418744 26.735212786605118 23.385717115570472 84-85 23.38933633714445 26.40631080441648 26.711051910778004 23.49330094766106 86-87 23.487882502817236 26.470077762255674 26.62068054853226 23.42135918639483 88-89 23.40814039581374 26.524973485682267 26.52332259420087 23.543563524303124 90-91 23.40306094369925 26.6062949973233 26.471559440244558 23.519084618732897 92-93 23.552203513024836 26.09083880740143 26.739347984151447 23.617609695422292 94-95 23.364854649577584 26.073224774733987 26.807818860660003 23.754101715028426 96-97 23.588544620057096 25.958377408809724 26.70992629338021 23.743151677752966 98-99 23.527349018132632 25.961857844333903 26.41764980130542 24.093143336228046 100-101 24.144634428323435 25.42663756573621 26.619640524397564 23.80908748154279 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 208.0 2 199.0 3 273.0 4 233.5 5 219.5 6 397.5 7 855.0 8 1362.5 9 1583.0 10 1397.5 11 1150.5 12 1184.5 13 1274.5 14 1338.5 15 1505.5 16 1666.5 17 1769.0 18 1927.0 19 2163.0 20 2461.0 21 2902.5 22 3547.5 23 4479.0 24 5686.5 25 7077.5 26 8896.5 27 11191.0 28 13392.0 29 15926.0 30 18974.5 31 22535.0 32 27192.0 33 30329.5 34 32044.0 35 34825.5 36 38407.5 37 42222.0 38 45689.5 39 48721.5 40 52113.0 41 56825.5 42 60845.0 43 63900.0 44 66987.0 45 70963.0 46 74019.0 47 75329.0 48 76305.0 49 75940.5 50 75374.0 51 74242.5 52 73284.0 53 73688.5 54 74505.5 55 71912.0 56 66334.5 57 61342.0 58 56832.0 59 50595.0 60 42366.0 61 35689.5 62 29896.5 63 23478.0 64 18049.0 65 14348.5 66 11204.5 67 8815.0 68 6853.0 69 5002.5 70 3900.5 71 2956.0 72 2191.5 73 1692.0 74 1274.0 75 954.5 76 693.0 77 468.0 78 306.5 79 192.5 80 104.0 81 60.5 82 39.0 83 24.0 84 15.0 85 15.5 86 13.5 87 8.5 88 5.5 89 5.0 90 4.5 91 3.0 92 2.5 93 1.5 94 1.5 95 2.0 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3016005837946955 2 0.03461154294957368 3 0.04261421183964852 4 0.044564862381604266 5 0.059369799828242725 6 0.05946983318936865 7 0.03096032526847704 8 0.0321107089214253 9 0.04126376146444839 10-11 0.033711242699440265 12-13 0.0352117431163293 14-15 0.0293097748098991 16-17 0.02278259799643181 18-19 0.027459157629069295 20-21 0.03998833611009272 22-23 0.032810942449306844 24-25 0.054443156792790395 26-27 0.04641547956243407 28-29 0.038687902415455555 30-31 0.038837952457144456 32-33 0.03391130942169213 34-35 0.033186067553529104 36-37 0.03218573394226974 38-39 0.020056688905750065 40-41 0.019231413676461098 42-43 0.020181730607157486 44-45 0.009328110924993485 46-47 0.016405471224653422 48-49 0.014479829022979163 50-51 0.007177393660785873 52-53 0.004976659716015291 54-55 0.006702235195437678 56-57 0.005401801500800517 58-59 0.007177393660785873 60-61 0.006677226855156195 62-63 0.004226409507570775 64-65 0.008877960799926775 66-67 0.002525842368429871 68-69 0.00755251876500813 70-71 0.014304770641008777 72-73 0.014854954127201421 74-75 0.011528844869764066 76-77 0.010178394494563937 78-79 0.006577193494030259 80-81 0.007252418681630324 82-83 0.012054020015675227 84-85 0.004751584653481937 86-87 0.0017005671391409035 88-89 0.02065688907250568 90-91 0.030160058379469553 92-93 0.01432977898129026 94-95 0.023707906586846714 96-97 0.019731580482090776 98-99 0.01425475396044581 100-101 0.1073357964881288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1999333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.3650000406048 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90009152547508 49.64434119745711 2 11.161907977913948 13.69900967035833 3 3.3712672691220824 6.206334483196987 4 1.4767554011243487 3.624843809998361 5 0.8009113885523367 2.4573963695517493 6 0.47814044295604924 1.760465298084766 7 0.3336550818980102 1.4332320879953575 8 0.2472678255589177 1.2138872100370595 9 0.18188188080450418 1.004506346065831 >10 0.9507914528693542 10.42995348988676 >50 0.06340465098900623 2.6909940904940566 >100 0.030239216057005906 3.3590703181693984 >500 0.002457257786361 1.0887513496419483 >1k 0.0012286288931804999 1.3872142790622835 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4865 0.2433311509388381 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3886 0.19436482066769267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.1313438031583533 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.001668056296775E-5 0.0 5 0.0 0.0 0.0 5.001668056296775E-5 0.0 6 0.0 0.0 0.0 5.001668056296775E-5 0.0 7 0.0 0.0 0.0 5.001668056296775E-5 0.0 8 0.0 0.0 0.0 5.001668056296775E-5 0.0 9 0.0 0.0 0.0 5.001668056296775E-5 0.0 10-11 0.0 0.0 0.0 5.001668056296775E-5 0.0 12-13 0.0 0.0 0.0 5.001668056296775E-5 0.0 14-15 0.0 0.0 0.0 1.000333611259355E-4 5.001668056296775E-5 16-17 0.0 0.0 0.0 1.5005004168890324E-4 1.000333611259355E-4 18-19 0.0 0.0 0.0 2.00066722251871E-4 1.000333611259355E-4 20-21 0.0 0.0 0.0 2.00066722251871E-4 1.5005004168890324E-4 22-23 0.0 0.0 0.0 3.251084236592903E-4 1.5005004168890324E-4 24-25 0.0 0.0 0.0 4.751584653481936E-4 1.5005004168890324E-4 26-27 0.0 0.0 0.0 9.503169306963872E-4 1.5005004168890324E-4 28-29 0.0 0.0 0.0 0.0024758256878669037 1.750583819703871E-4 30-31 0.0 0.0 0.0 0.008402802334578581 2.00066722251871E-4 32-33 0.0 0.0 0.0 0.02110703919757239 2.00066722251871E-4 34-35 0.0 0.0 0.0 0.041213744783885425 2.00066722251871E-4 36-37 0.0 0.0 0.0 0.07690064636556292 2.00066722251871E-4 38-39 0.0 0.0 0.0 0.1322691117487682 2.00066722251871E-4 40-41 0.0 0.0 0.0 0.1918389782992628 2.00066722251871E-4 42-43 0.0 0.0 0.0 0.25340951207227613 2.00066722251871E-4 44-45 0.0 0.0 0.0 0.33148555043106875 2.00066722251871E-4 46-47 0.0 0.0 0.0 0.426492235160426 2.00066722251871E-4 48-49 0.0 0.0 0.0 0.531102122557873 2.00066722251871E-4 50-51 0.0 0.0 0.0 0.6408137113727428 2.00066722251871E-4 52-53 0.0 0.0 0.0 0.7470491408884863 2.7509174309632263E-4 54-55 0.0 0.0 0.0 0.8653135820796236 3.001000833778065E-4 56-57 0.0 0.0 0.0 1.0090365136773114 3.001000833778065E-4 58-59 0.0 0.0 0.0 1.165763782221371 3.001000833778065E-4 60-61 0.0 5.001668056296775E-5 0.0 1.336270646260528 3.001000833778065E-4 62-63 0.0 5.001668056296775E-5 0.0 1.508903219223611 3.001000833778065E-4 64-65 0.0 5.001668056296775E-5 0.0 1.6869125853472133 3.001000833778065E-4 66-67 0.0 5.001668056296775E-5 0.0 1.8929813092666405 3.001000833778065E-4 68-69 0.0 5.001668056296775E-5 0.0 2.1288099581210336 3.001000833778065E-4 70-71 0.0 5.001668056296775E-5 0.0 2.3720910923793084 3.001000833778065E-4 72-73 0.0 5.001668056296775E-5 0.0 2.628926747070148 3.751251042222581E-4 74-75 0.0 5.001668056296775E-5 0.0 2.8826363592258017 4.00133444503742E-4 76-77 0.0 5.001668056296775E-5 0.0 3.159878819586332 4.00133444503742E-4 78-79 0.0 5.001668056296775E-5 0.0 3.4664060464164796 4.00133444503742E-4 80-81 0.0 5.001668056296775E-5 0.0 3.8013927644869563 4.251417847852259E-4 82-83 0.0 5.001668056296775E-5 0.0 4.1431317344334335 4.501501250667098E-4 84-85 0.0 5.001668056296775E-5 0.0 4.486196146414829 4.751584653481936E-4 86-87 0.0 5.001668056296775E-5 0.0 4.848466963732404 5.001668056296775E-4 88-89 0.0 5.001668056296775E-5 0.0 5.236046221414842 5.001668056296775E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 49.52412 1 GTATCAA 4955 0.0 44.253746 1 ATCAACG 6490 0.0 33.75278 3 TATCAAC 6540 0.0 33.422073 2 TCAACGC 6665 0.0 32.795254 4 GTGGTAT 1240 0.0 32.676323 1 CAACGCA 6835 0.0 31.840527 5 TGGTATC 1270 0.0 31.054726 2 AACGCAG 7040 0.0 30.909487 6 ACGCAGA 8200 0.0 26.474346 7 CGCAGAG 8240 0.0 26.23053 8 TAAGGTG 1000 0.0 24.709085 5 GTACATG 6235 0.0 23.777153 1 GCAGAGT 9015 0.0 23.395336 9 TACATGG 6205 0.0 23.203535 2 GGACCGA 605 0.0 22.774118 6 GTAAGGT 915 0.0 22.330612 4 ACATGGG 6480 0.0 21.045546 3 GAGTACT 5310 0.0 20.84259 12-13 AGGACCG 730 0.0 20.829575 5 >>END_MODULE