FastQCFastQC Report
Thu 26 May 2016
SRR938633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999333
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47080.23547853209045216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40820.20416809005803435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.13949652209011706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.049.202071
GTATCAA47850.043.7301331
ATCAACG62950.033.0777663
TCAACGC64150.032.459014
TATCAAC65250.032.1416472
CAACGCA65700.031.6208765
AACGCAG68050.030.5704446
GTGGTAT12700.027.3356361
ACGCAGA80500.025.7244667
CGCAGAG82300.025.1618428
TACATGG58450.023.5951922
GTACATG61000.023.5443741
TGGTATC13800.023.0890352
GCAGAGT91700.022.0645989
ACATGGG62550.021.2808283
TAAGGTG10150.021.076785
AGAGTAC84650.020.25517710-11
AGTACTT58450.020.2334812-13
GAGTACT55550.020.17825912-13
GTTACGT1707.906499E-519.5752333
Produced by FastQC (version 0.11.5)