##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1999333 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77600329709958 31.0 31.0 34.0 30.0 34.0 2 32.02051934320096 31.0 31.0 34.0 30.0 34.0 3 32.08750468281172 33.0 31.0 34.0 30.0 34.0 4 35.758787055483005 37.0 35.0 37.0 35.0 37.0 5 35.62330387184126 37.0 35.0 37.0 33.0 37.0 6 35.66071684906917 37.0 35.0 37.0 33.0 37.0 7 35.59185738443771 37.0 35.0 37.0 33.0 37.0 8 35.57878002313772 37.0 35.0 37.0 33.0 37.0 9 37.15004003835279 39.0 37.0 39.0 33.0 39.0 10-11 37.08197283794145 39.0 37.0 39.0 33.0 39.0 12-13 36.9648622815709 39.0 37.0 39.0 33.0 39.0 14-15 38.02102901317589 40.0 37.0 41.0 33.0 41.0 16-17 37.92540712327561 40.0 37.0 41.0 33.0 41.0 18-19 37.908124859640694 40.0 37.0 41.0 33.0 41.0 20-21 37.89790520138466 40.0 37.0 41.0 32.5 41.0 22-23 37.926700054468164 40.0 37.0 41.0 32.5 41.0 24-25 37.846592838711715 40.0 37.0 41.0 32.0 41.0 26-27 37.66622968760082 40.0 37.0 41.0 32.0 41.0 28-29 37.529521845535484 39.5 36.5 41.0 32.0 41.0 30-31 37.28934124530531 39.0 36.0 41.0 31.0 41.0 32-33 37.07631620145318 39.0 36.0 40.5 31.0 41.0 34-35 36.9003252584737 39.0 36.0 40.5 30.0 41.0 36-37 36.91952491155801 39.0 36.0 41.0 30.0 41.0 38-39 36.77272720452271 39.0 35.0 41.0 30.0 41.0 40-41 36.59150051542189 39.0 35.0 40.0 30.0 41.0 42-43 36.36673905747567 39.0 35.0 40.0 30.0 41.0 44-45 36.09349768147677 38.0 35.0 40.0 29.5 41.0 46-47 35.7422077762934 38.0 35.0 40.0 28.5 41.0 48-49 35.35133391986227 38.0 34.0 40.0 27.0 41.0 50-51 34.96680342894355 38.0 34.0 40.0 26.0 41.0 52-53 34.42643471597778 38.0 33.0 40.0 24.0 41.0 54-55 34.07723975945978 37.0 33.0 40.0 24.0 41.0 56-57 33.68393759318732 37.0 32.0 40.0 23.0 41.0 58-59 33.197373073920154 36.5 31.5 39.0 22.0 41.0 60-61 32.762952694723694 36.0 31.0 39.0 21.0 40.0 62-63 32.25285832825247 35.0 31.0 39.0 19.5 40.0 64-65 31.722223361491057 35.0 30.0 38.0 18.0 40.0 66-67 31.20397777658849 35.0 29.5 38.0 14.0 40.0 68-69 30.615163657079634 34.0 29.0 37.0 10.0 39.5 70-71 30.021695235360994 34.0 28.5 36.5 7.5 39.0 72-73 29.410472392542914 34.0 28.0 36.0 6.5 39.0 74-75 28.768703612654818 33.0 26.5 35.5 2.0 38.0 76-77 27.516398468889374 31.5 26.0 34.5 2.0 36.0 78-79 28.12086055699576 33.0 26.0 35.0 2.0 37.0 80-81 27.794402183128074 33.0 26.0 35.0 2.0 36.5 82-83 27.280936442303506 32.5 25.5 35.0 2.0 36.0 84-85 26.668539457909212 32.0 25.0 35.0 2.0 36.0 86-87 25.99042430650622 32.0 23.5 34.5 2.0 35.0 88-89 25.214812389932042 31.0 20.0 34.0 2.0 35.0 90-91 24.637558125634897 31.0 18.5 34.0 2.0 35.0 92-93 23.827676530122794 31.0 9.5 34.0 2.0 35.0 94-95 23.01062654395241 30.0 2.0 34.0 2.0 35.0 96-97 22.004119373811164 29.5 2.0 34.0 2.0 35.0 98-99 20.79077822453788 29.0 2.0 34.0 2.0 35.0 100-101 17.701525958907297 23.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3874430990836366 1101 2 0.3528588662860699 1101 3 0.24843103752752427 1101 4 0.27354515474756624 1101 5 0.22871733713164133 1101 6 0.24098978594312115 1101 7 0.3294110563765287 1101 8 0.26221987771814526 1101 9 0.41487583173679354 1101 10-11 0.3367586268354472 1101 12-13 0.4016392354957645 1101 14-15 0.5069288918532351 1101 16-17 0.5169764187340817 1101 18-19 0.5856745796651026 1101 20-21 0.5164258414353853 1101 22-23 0.5942053862418888 1101 24-25 0.6188346967867062 1101 26-27 0.668672113228169 1101 28-29 0.5600040865746365 1101 30-31 0.6888923654458381 1101 32-33 0.5564417738207155 1101 34-35 0.41267410737220445 1101 36-37 0.7628168149937551 1101 38-39 1.0043747500858302 1101 40-41 1.0298027760486477 1101 42-43 0.9755696952921795 1101 44-45 1.064599091434566 1101 46-47 1.2332287515795883 1101 48-49 1.2942462489760445 1101 50-51 1.3965738349530454 1101 52-53 1.4417823891437607 1101 54-55 1.4263542949668562 1101 56-57 1.4007621367566188 1101 58-59 1.621870408814992 1101 60-61 1.5410151894985447 1101 62-63 1.6735634220348459 1101 64-65 1.717319139707751 1101 66-67 1.8125355706856716 1101 68-69 1.7609702740556088 1101 70-71 1.9697221193288783 1101 72-73 1.9556869365027403 1101 74-75 1.9851326587803904 1101 76-77 1.8259047489108546 1101 78-79 2.1861761417434806 1101 80-81 2.5942336993809434 1101 82-83 2.7855026884823175 1101 84-85 3.0887619884996944 1101 86-87 3.1349883971808516 1101 88-89 3.2806498252303022 1101 90-91 3.3764041594979517 1101 92-93 3.80852734153574 1101 94-95 3.8642905452994896 1101 96-97 4.330633835991112 1101 98-99 4.810592084309118 1101 100-101 5.011070713179812 1102 1 0.3157548591582575 1102 2 0.28792823932167977 1102 3 0.23924370644188286 1102 4 0.15990409347203638 1102 5 0.15486112492639137 1102 6 0.24703616689134833 1102 7 0.2279988232897736 1102 8 0.1652775781354805 1102 9 0.38600017555449995 1102 10-11 0.3773646775268915 1102 12-13 0.3531576989645657 1102 14-15 0.6050928018791382 1102 16-17 0.5820366202783802 1102 18-19 0.6126667304057847 1102 20-21 0.5631903200314241 1102 22-23 0.5314814221633881 1102 24-25 0.5344876176418225 1102 26-27 0.5089067860354817 1102 28-29 0.389959002930226 1102 30-31 0.3292251410112357 1102 32-33 0.4589147007832395 1102 34-35 0.6384228874496003 1102 36-37 0.6803981639404384 1102 38-39 0.7942876157250396 1102 40-41 0.679038675529938 1102 42-43 0.6611395793553854 1102 44-45 0.5895115571842879 1102 46-47 0.5784482805201634 1102 48-49 0.7699529627569675 1102 50-51 0.8682645157622986 1102 52-53 1.0095165270339592 1102 54-55 0.8302217715915035 1102 56-57 1.0506583732252892 1102 58-59 1.061397900438088 1102 60-61 1.2257144050919493 1102 62-63 1.4332529519933104 1102 64-65 1.314233914429746 1102 66-67 1.486100980141348 1102 68-69 1.4790566262948914 1102 70-71 1.6243358832133517 1102 72-73 1.7208057452267695 1102 74-75 1.7897270513950012 1102 76-77 1.769191980699265 1102 78-79 2.1670292254626844 1102 80-81 2.3207286368839313 1102 82-83 2.3950921422021203 1102 84-85 2.537117430008845 1102 86-87 2.656994393445782 1102 88-89 2.754109627508324 1102 90-91 3.0456529696968957 1102 92-93 3.3808233355779045 1102 94-95 3.4767501149145374 1102 96-97 3.7113718605993746 1102 98-99 3.9886589769912426 1102 100-101 4.275190187693866 1103 1 0.27778340368768895 1103 2 0.32661234734443667 1103 3 0.3873095010407397 1103 4 0.2723599675281605 1103 5 0.16848536489467136 1103 6 0.19328504682162162 1103 7 0.3307081256762885 1103 8 0.2146270206769998 1103 9 0.3312027837231071 1103 10-11 0.35617461763263947 1103 12-13 0.494365959446057 1103 14-15 0.6168079015544308 1103 16-17 0.6054703130238224 1103 18-19 0.5470878486764192 1103 20-21 0.6055040653175254 1103 22-23 0.47053652872066465 1103 24-25 0.4777112176201683 1103 26-27 0.4884057982780803 1103 28-29 0.5087571353613214 1103 30-31 0.621237144315991 1103 32-33 0.6896412046460583 1103 34-35 0.5841809454015277 1103 36-37 0.7542205841993379 1103 38-39 0.7079134277210102 1103 40-41 0.7370447505436033 1103 42-43 0.9239738754101268 1103 44-45 0.8115133073751153 1103 46-47 0.7398754371238141 1103 48-49 0.8622764022138085 1103 50-51 0.9510169266414294 1103 52-53 1.288114767410974 1103 54-55 1.1133037842760203 1103 56-57 1.1403128590063076 1103 58-59 1.41667091798017 1103 60-61 1.5357479591496173 1103 62-63 1.573640264681849 1103 64-65 1.4364887610784471 1103 66-67 1.670277124097165 1103 68-69 1.6948829277154829 1103 70-71 1.516774255361213 1103 72-73 1.5536459276518535 1103 74-75 1.915784458352796 1103 76-77 1.7743491048667472 1103 78-79 2.060239637875412 1103 80-81 2.4783532331784883 1103 82-83 2.6361879153690033 1103 84-85 2.6734192174043265 1103 86-87 2.9916825817537394 1103 88-89 3.27266042758308 1103 90-91 3.414416604853727 1103 92-93 3.4776934284498076 1103 94-95 3.577164256875623 1103 96-97 3.8143029935597283 1103 98-99 4.072329277370798 1103 100-101 4.50653414909884 1104 1 0.3154026076595322 1104 2 0.28527603609172303 1104 3 0.2330537583830079 1104 4 0.16582309773516357 1104 5 0.153813459206674 1104 6 0.18548304237879876 1104 7 0.2385629292550817 1104 8 0.2714032906525716 1104 9 0.22285246205730402 1104 10-11 0.3242631325577179 1104 12-13 0.30766075782912594 1104 14-15 0.40127834065321366 1104 16-17 0.5168074541490597 1104 18-19 0.5296718311745252 1104 20-21 0.5156576157888111 1104 22-23 0.5205954015613443 1104 24-25 0.5028859418679588 1104 26-27 0.4039590526412269 1104 28-29 0.5310123921226833 1104 30-31 0.5735627718619867 1104 32-33 0.616002165518168 1104 34-35 0.6674794601702558 1104 36-37 0.7040265263271479 1104 38-39 0.677100862248281 1104 40-41 0.6832864503149665 1104 42-43 0.6995803137776591 1104 44-45 0.7419980130650359 1104 46-47 0.7488684890461741 1104 48-49 0.9315333535989154 1104 50-51 0.849983915755665 1104 52-53 1.082679703733291 1104 54-55 1.0843515765144218 1104 56-57 1.1452065812010588 1104 58-59 1.2199783809395939 1104 60-61 1.3795394903606422 1104 62-63 1.5141156733940662 1104 64-65 1.3613407972127796 1104 66-67 1.7273770443572367 1104 68-69 1.8177037005072094 1104 70-71 1.6606562306502575 1104 72-73 2.0165633745790963 1104 74-75 1.9025070993198732 1104 76-77 1.849241010963663 1104 78-79 2.252215011107573 1104 80-81 2.512754095283743 1104 82-83 2.835765522281246 1104 84-85 2.772010839540517 1104 86-87 2.983300628714904 1104 88-89 2.931146956587124 1104 90-91 3.1247475556110125 1104 92-93 3.6808655861063464 1104 94-95 3.923096979470884 1104 96-97 4.191084483313208 1104 98-99 4.376894538425844 1104 100-101 4.563704307845036 1105 1 0.2704552079150879 1105 2 0.31979790764557947 1105 3 0.251552244265838 1105 4 0.18372293844653598 1105 5 0.04055749463526581 1105 6 0.1537758533854685 1105 7 0.21086071146726937 1105 8 0.17334801224755836 1105 9 0.3022622189233388 1105 10-11 0.30712122564528954 1105 12-13 0.2605813410705622 1105 14-15 0.43500902034304545 1105 16-17 0.46454503051132434 1105 18-19 0.3472530946309078 1105 20-21 0.3353551424390204 1105 22-23 0.3509022509723323 1105 24-25 0.3271643293769273 1105 26-27 0.47077450538851906 1105 28-29 0.5413941597013192 1105 30-31 0.5482726390047645 1105 32-33 0.6073218105320848 1105 34-35 0.5572184718805957 1105 36-37 0.7101075009634954 1105 38-39 0.8766510031574484 1105 40-41 0.8165060060572387 1105 42-43 0.8435334724186276 1105 44-45 0.7940381327940713 1105 46-47 0.822561359567274 1105 48-49 1.0577354576091338 1105 50-51 1.0337714842410222 1105 52-53 1.0977298533945827 1105 54-55 1.174622717266054 1105 56-57 1.2478444708893122 1105 58-59 1.478922174809604 1105 60-61 1.4628684476327791 1105 62-63 1.5712267728356792 1105 64-65 1.737888610057393 1105 66-67 1.7289716810566382 1105 68-69 1.7235938788754623 1105 70-71 1.8222449159064311 1105 72-73 1.895841849609294 1105 74-75 2.054503458436958 1105 76-77 2.0877030880173457 1105 78-79 2.189894648419621 1105 80-81 2.4722982847496873 1105 82-83 2.5971319036287177 1105 84-85 2.618421594148508 1105 86-87 2.778014346791533 1105 88-89 2.8769135549872367 1105 90-91 3.178973955512987 1105 92-93 3.4732144807132848 1105 94-95 3.3598874011481428 1105 96-97 3.759457813845863 1105 98-99 4.08479420409477 1105 100-101 4.382509118011502 1106 1 0.1489484936392813 1106 2 0.1031154378448349 1106 3 0.12252018329041192 1106 4 0.13050550259609395 1106 5 0.027795847020477993 1106 6 0.04178767812459938 1106 7 0.12565467737245228 1106 8 0.11063624143882578 1106 9 0.05937098560916354 1106 10-11 0.24205461161656672 1106 12-13 0.08218710398595874 1106 14-15 0.08129469635881748 1106 16-17 0.02919582824647904 1106 18-19 0.18705878070429804 1106 20-21 0.3320716154144847 1106 22-23 0.2755903887521569 1106 24-25 0.19805683634146476 1106 26-27 0.21215204519890563 1106 28-29 0.4133569324522526 1106 30-31 0.4574467989418878 1106 32-33 0.3115497657475288 1106 34-35 0.35986094389024714 1106 36-37 0.5114982942573292 1106 38-39 0.4114325850452971 1106 40-41 0.5106312350264375 1106 42-43 0.607534050496092 1106 44-45 0.23779988967960008 1106 46-47 0.00447693440774799 1106 48-49 0.46223987954929413 1106 50-51 0.15919902070279335 1106 52-53 0.43707601487793823 1106 54-55 0.55274918228708 1106 56-57 0.6504491093735254 1106 58-59 0.9780125252631962 1106 60-61 0.8674151679243565 1106 62-63 0.9761265668662062 1106 64-65 0.7669682566781653 1106 66-67 0.9205087209005107 1106 68-69 0.866553377029021 1106 70-71 1.0552540793742864 1106 72-73 1.4235674790028874 1106 74-75 1.6596886488441633 1106 76-77 1.690647053276301 1106 78-79 2.0373061655557407 1106 80-81 1.815986254511067 1106 82-83 2.1387659359423097 1106 84-85 2.244430732942323 1106 86-87 2.508554696481408 1106 88-89 2.7011791792345328 1106 90-91 2.678612187112911 1106 92-93 2.3460842006159517 1106 94-95 2.872485965119111 1106 96-97 3.415389276417109 1106 98-99 3.8621208777876603 1106 100-101 4.293919685784623 1107 1 0.18977929920385606 1107 2 0.211606034178061 1107 3 0.2201966545802776 1107 4 0.19310172755008637 1107 5 0.13580107505183037 1107 6 0.10692704030110178 1107 7 0.1501501787698345 1107 8 0.17556893214085534 1107 9 0.21823505862910508 1107 10-11 0.20111221263098145 1107 12-13 0.36499522569320675 1107 14-15 0.45525159033199714 1107 16-17 0.40381058436037875 1107 18-19 0.4362606526294641 1107 20-21 0.407482159926154 1107 22-23 0.3135319225159918 1107 24-25 0.29454098984630406 1107 26-27 0.27150942307234516 1107 28-29 0.357554602133888 1107 30-31 0.25639479296699363 1107 32-33 0.44342910447221584 1107 34-35 0.5865973283459809 1107 36-37 0.5406523908942518 1107 38-39 0.4939161797802001 1107 40-41 0.5267365856385169 1107 42-43 0.6009540528082482 1107 44-45 0.555368731236662 1107 46-47 0.6080698067421224 1107 48-49 0.5990215298749249 1107 50-51 0.45168434496368093 1107 52-53 0.7708935696126176 1107 54-55 0.7046378063184093 1107 56-57 0.7557871055456289 1107 58-59 0.8809447140815578 1107 60-61 0.9862110095383088 1107 62-63 1.143543077095643 1107 64-65 1.3851591082655723 1107 66-67 1.5043559803587705 1107 68-69 1.4637752169510634 1107 70-71 1.6036634889131918 1107 72-73 1.7958462281289478 1107 74-75 1.9703739081446123 1107 76-77 1.8600894485918111 1107 78-79 2.054564036504658 1107 80-81 2.111515338073321 1107 82-83 2.3447833330469834 1107 84-85 2.477286749215466 1107 86-87 2.6502673309210856 1107 88-89 2.8195566315372353 1107 90-91 2.9886134349406817 1107 92-93 3.2357710646596125 1107 94-95 3.467859001396036 1107 96-97 3.6523006182540207 1107 98-99 3.961595459719483 1107 100-101 4.202333370968688 1108 1 0.16813743736605247 1108 2 0.19139413910318837 1108 3 0.14946775800869005 1108 4 0.07621599840756943 1108 5 0.09546951248815105 1108 6 0.04690532729227215 1108 7 0.13277098812399402 1108 8 0.09489790179183188 1108 9 0.2545885379806663 1108 10-11 0.1920313088357659 1108 12-13 0.29103528935659284 1108 14-15 0.35107965351754444 1108 16-17 0.40373724147051604 1108 18-19 0.43621629555235586 1108 20-21 0.43033615575220097 1108 22-23 0.36771255507426304 1108 24-25 0.3604652683630718 1108 26-27 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1.5847006326580768 2316 80-81 1.7299354354664906 2316 82-83 1.733316832425242 2316 84-85 2.0570678938085827 2316 86-87 2.204273816353062 2316 88-89 2.4150236235923934 2316 90-91 2.615351136074704 2316 92-93 2.565134304695057 2316 94-95 3.0861446061197526 2316 96-97 3.4167718433379726 2316 98-99 3.429930580083443 2316 100-101 4.013945440126772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 26.0 5 54.0 6 145.0 7 378.0 8 880.0 9 1595.0 10 2590.0 11 4044.0 12 5724.0 13 7868.0 14 10424.0 15 13706.0 16 17000.0 17 20846.0 18 20767.0 19 19741.0 20 20629.0 21 21681.0 22 23884.0 23 26950.0 24 30711.0 25 36050.0 26 42018.0 27 49566.0 28 58772.0 29 69415.0 30 81018.0 31 95718.0 32 112225.0 33 134792.0 34 166792.0 35 208808.0 36 260190.0 37 281462.0 38 145124.0 39 7723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.365650127142466 18.964284070879675 14.968999645881725 23.701066156096136 2 16.32900903389286 20.060606242563075 38.76602847973004 24.84435624381403 3 19.640218753720003 23.271242408691727 29.540205049101548 27.54833378848672 4 13.76128939001157 16.673310549068116 35.57646475099446 33.988935309925864 5 14.390804896952123 36.614139718943946 33.92934746691073 15.065707917193205 6 33.615310706120496 35.46582785358917 16.61679170003196 14.302069740258377 7 29.655990272755968 31.678764868083505 19.916042000007003 18.749202859153527 8 26.858507312188618 35.19138632734017 18.93566504429227 19.014441316178946 9 26.08554953076851 16.65470434389869 19.557222333648273 37.70252379168453 10-11 25.538967245576398 26.184207433178962 26.873587341378354 21.403237979866287 12-13 26.22542117796285 23.128113225760792 27.322136932667046 23.324328663609315 14-15 22.752988121538532 24.921511324026564 25.28420728312892 27.04129327130598 16-17 22.86164936006158 28.024046019347455 26.101004685062467 23.0132999355285 18-19 22.913741732867912 26.544552608294868 28.063809280395013 22.47789637844221 20-21 23.233848488470905 25.8885088176907 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23.203336516064624 26.45092283368787 27.242237528453266 23.10350312179424 48-49 23.123611724510123 26.338758976118537 27.273295644097306 23.264333655274033 50-51 23.16297485211318 26.56113313790149 27.189292629091806 23.086599380893528 52-53 23.457147958844274 26.422411874360098 27.02323724962275 23.097202917172876 54-55 23.13178945178217 26.447470231322146 27.237533717494784 23.1832065994009 56-57 23.050612379228472 26.60292207451185 27.22107822958957 23.12538731667011 58-59 23.142643071464335 26.29636983934142 27.136199922674216 23.424787166520034 60-61 22.87898013987665 26.532648638320882 27.360224634915742 23.228146586886727 62-63 22.913141532701157 26.63025619043951 27.30370578587959 23.152896490979742 64-65 22.658956762080155 26.4746793055484 27.29132665744026 23.57503727493119 66-67 22.66444025192465 26.568451999871957 27.230724523439875 23.536383224763522 68-69 22.934840970594422 26.266829687823158 27.32333282399642 23.474996517586003 70-71 22.803805068990506 26.36534284183775 27.060249593239345 23.770602495932394 72-73 22.89531058608046 26.367693625824213 27.037992170388826 23.699003617706506 74-75 22.816734380916035 26.6813232212943 26.80403914705554 23.69790325073412 76-77 22.811507637797206 26.45732351739305 27.04859570666817 23.682573138141567 78-79 22.845994139045374 26.578263850994304 27.060949826767228 23.514792183193094 80-81 22.899937129032534 26.671444926883115 26.907398617438915 23.521219326645436 82-83 22.856837752186753 26.654769240618776 26.90410501212417 23.584287995070305 84-85 22.8506511207981 26.52363767322164 26.922213135458634 23.703498070521622 86-87 22.935856678123987 26.52330511017834 26.810830168898775 23.730008042798893 88-89 22.779827767517407 26.714415936318748 26.587973736234467 23.917782559929375 90-91 22.77142990760083 26.674417656651816 26.75106892887537 23.80308350687198 92-93 22.834711045555782 26.309976997282586 26.87519162679079 23.980120330370838 94-95 22.52238621580297 26.296494881042825 26.971244910177543 24.209873992976657 96-97 22.658106478510582 26.133215427345018 26.953589021938818 24.25508907220558 98-99 22.523786682858734 26.22959757078986 26.694652666664332 24.551963079687074 100-101 22.648968596228507 25.883803144478556 26.937589593230477 24.52963866606246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 332.0 1 224.5 2 191.0 3 270.0 4 228.0 5 209.5 6 397.0 7 892.5 8 1336.0 9 1507.0 10 1351.5 11 1093.0 12 1131.0 13 1240.0 14 1364.0 15 1490.0 16 1625.0 17 1817.5 18 1995.0 19 2179.5 20 2435.5 21 2869.5 22 3531.0 23 4408.0 24 5550.5 25 6998.5 26 8914.0 27 11317.5 28 13584.0 29 16133.5 30 19220.5 31 22877.5 32 27860.0 33 30822.0 34 32020.5 35 34954.0 36 38900.5 37 42723.5 38 46024.0 39 48801.0 40 52141.0 41 57091.5 42 61432.0 43 64938.0 44 68149.0 45 72027.0 46 74755.5 47 76453.5 48 77741.0 49 77074.5 50 76304.5 51 74955.5 52 73585.5 53 73989.5 54 75599.5 55 72845.5 56 66593.5 57 61097.5 58 56355.0 59 49896.5 60 40874.5 61 33975.0 62 28864.0 63 22204.5 64 16605.5 65 13049.0 66 9780.0 67 7617.0 68 5934.5 69 4411.0 70 3295.0 71 2396.5 72 1839.5 73 1412.0 74 1080.0 75 850.0 76 578.5 77 353.0 78 211.0 79 134.0 80 83.5 81 48.0 82 26.5 83 11.0 84 7.5 85 5.0 86 3.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.001668056296775E-5 2 0.04296432860358929 3 3.501167639407743E-4 4 0.0 5 0.1004835112510022 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012004003335112261 26-27 0.012629211842149357 28-29 0.0 30-31 0.0 32-33 5.751918264741291E-4 34-35 0.015730246037053357 36-37 0.003676226021378129 38-39 0.013129378647779036 40-41 0.007377460383037743 42-43 0.006552185153748775 44-45 0.0032260758963114197 46-47 1.000333611259355E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.5008340281483873E-4 68-69 7.502502084445163E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010503502918223228 84-85 0.004401467889541162 86-87 0.0014504837363260646 88-89 2.5008340281483873E-5 90-91 9.253085904149033E-4 92-93 2.00066722251871E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.503169306963872E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1999333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.64026857418222 #Duplication Level Percentage of deduplicated Percentage of total 1 78.90438212206239 46.2697415931764 2 12.23653309575984 14.351071743044526 3 3.7033769963035814 6.515010650840707 4 1.6791756982078299 3.938692557045883 5 0.8843689301228496 2.592981579053304 6 0.5654760380064628 1.989580004457807 7 0.38562750276335506 1.5829310232144036 8 0.26637292026043397 1.2496143667968858 9 0.19544723214870008 1.031497036675228 >10 1.0699858447946635 11.348060972659404 >50 0.06912236366914654 2.7596980306987082 >100 0.0360345425418948 3.7547396732199694 >500 0.0028164904341710256 1.2081185872508162 >1k 0.0012802229246231904 1.4082621818660015 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4708 0.23547853209045216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4082 0.20416809005803435 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2789 0.13949652209011706 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.001668056296775E-5 7 0.0 0.0 0.0 0.0 5.001668056296775E-5 8 0.0 0.0 0.0 0.0 5.001668056296775E-5 9 0.0 0.0 0.0 5.001668056296775E-5 5.001668056296775E-5 10-11 0.0 0.0 0.0 5.001668056296775E-5 5.001668056296775E-5 12-13 0.0 0.0 0.0 5.001668056296775E-5 1.000333611259355E-4 14-15 0.0 0.0 0.0 5.001668056296775E-5 1.750583819703871E-4 16-17 0.0 0.0 0.0 1.000333611259355E-4 2.5008340281483873E-4 18-19 0.0 0.0 0.0 2.00066722251871E-4 3.001000833778065E-4 20-21 0.0 0.0 0.0 2.00066722251871E-4 3.001000833778065E-4 22-23 0.0 0.0 0.0 3.251084236592903E-4 3.001000833778065E-4 24-25 0.0 0.0 0.0 4.751584653481936E-4 3.001000833778065E-4 26-27 0.0 0.0 0.0 9.503169306963872E-4 3.001000833778065E-4 28-29 0.0 0.0 0.0 0.0024758256878669037 3.001000833778065E-4 30-31 0.0 0.0 0.0 0.008427810674860066 3.001000833778065E-4 32-33 0.0 0.0 0.0 0.021282097579542778 3.001000833778065E-4 34-35 0.0 0.0 0.0 0.04148883652698175 3.001000833778065E-4 36-37 0.0 0.0 0.0 0.07735079649062962 3.001000833778065E-4 38-39 0.0 0.0 0.0 0.1330943869780572 3.001000833778065E-4 40-41 0.0 0.0 0.0 0.19343951207727778 3.001000833778065E-4 42-43 0.0 0.0 0.0 0.2560103794615504 3.001000833778065E-4 44-45 0.0 0.0 0.0 0.3347366346676617 3.001000833778065E-4 46-47 0.0 0.0 0.0 0.43064361964715236 3.001000833778065E-4 48-49 0.0 0.0 0.0 0.5357036571696661 3.001000833778065E-4 50-51 0.0 0.0 0.0 0.64599043781101 3.001000833778065E-4 52-53 0.0 0.0 0.0 0.7527760508129462 3.001000833778065E-4 54-55 0.0 0.0 0.0 0.8714656337888687 3.001000833778065E-4 56-57 0.0 0.0 0.0 1.0152635904074008 3.001000833778065E-4 58-59 0.0 0.0 0.0 1.1722409423542752 3.001000833778065E-4 60-61 0.0 0.0 0.0 1.3436481066435655 3.001000833778065E-4 62-63 0.0 0.0 0.0 1.5175561049610047 3.001000833778065E-4 64-65 0.0 0.0 0.0 1.6956154877651697 3.501167639407742E-4 66-67 0.0 0.0 0.0 1.9022343951707894 3.501167639407742E-4 68-69 0.0 0.0 0.0 2.1393134610392566 4.00133444503742E-4 70-71 0.0 0.0 0.0 2.3848453459228653 4.751584653481936E-4 72-73 0.0 0.0 0.0 2.6446069764266382 5.001668056296775E-4 74-75 0.0 0.0 0.0 2.9022679063467667 5.001668056296775E-4 76-77 0.0 0.0 0.0 3.1850372099095052 5.001668056296775E-4 78-79 0.0 0.0 0.0 3.498641797039313 5.001668056296775E-4 80-81 0.0 0.0 0.0 3.842481467569434 5.251751459111613E-4 82-83 0.0 0.0 0.0 4.19549919898286 5.501834861926453E-4 84-85 0.0 0.0 0.0 4.553218498369207 5.501834861926453E-4 86-87 0.0 0.0 0.0 4.929368944543005 5.501834861926453E-4 88-89 0.0 0.0 0.0 5.334854173866985 5.501834861926453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2890 0.0 49.20207 1 GTATCAA 4785 0.0 43.730133 1 ATCAACG 6295 0.0 33.077766 3 TCAACGC 6415 0.0 32.45901 4 TATCAAC 6525 0.0 32.141647 2 CAACGCA 6570 0.0 31.620876 5 AACGCAG 6805 0.0 30.570444 6 GTGGTAT 1270 0.0 27.335636 1 ACGCAGA 8050 0.0 25.724466 7 CGCAGAG 8230 0.0 25.161842 8 TACATGG 5845 0.0 23.595192 2 GTACATG 6100 0.0 23.544374 1 TGGTATC 1380 0.0 23.089035 2 GCAGAGT 9170 0.0 22.064598 9 ACATGGG 6255 0.0 21.280828 3 TAAGGTG 1015 0.0 21.07678 5 AGAGTAC 8465 0.0 20.255177 10-11 AGTACTT 5845 0.0 20.23348 12-13 GAGTACT 5555 0.0 20.178259 12-13 GTTACGT 170 7.906499E-5 19.575233 3 >>END_MODULE