FastQCFastQC Report
Thu 26 May 2016
SRR938632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012273
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44520.2212423463416743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37480.1862570337126225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.12468487128734522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.051.7005731
GTATCAA47950.044.6639821
ATCAACG63000.033.726653
TATCAAC63600.033.4084782
TCAACGC64900.032.5927934
CAACGCA66350.031.736445
AACGCAG69400.030.403376
ACGCAGA79350.026.1626387
TAAGGTG10400.026.0517545
CGCAGAG80500.025.670868
GTGGTAT11800.023.8489741
GTAAGGT9900.023.527034
GCAGAGT88600.022.6777349
GTACATG61300.022.1760431
TACATGG58850.022.1314742
ACATGGG60900.020.840123
GAGTACT52550.020.60891512-13
AGAGTAC82300.020.22881510-11
CAGAGTA84550.020.05565510-11
TGGTATC13450.019.4377782
Produced by FastQC (version 0.11.5)