##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938632_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2012273 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.986613148414754 31.0 31.0 34.0 28.0 34.0 2 31.164113914960843 31.0 31.0 34.0 27.0 34.0 3 31.219826037520754 31.0 31.0 34.0 27.0 34.0 4 34.888544943951445 37.0 35.0 37.0 32.0 37.0 5 34.86976220423372 37.0 35.0 37.0 32.0 37.0 6 34.82995398735659 37.0 35.0 37.0 32.0 37.0 7 34.73964367657867 37.0 35.0 37.0 31.0 37.0 8 34.694364035098616 37.0 35.0 37.0 31.0 37.0 9 36.160896160709804 39.0 35.0 39.0 31.0 39.0 10-11 35.983393654837094 38.0 35.0 39.0 30.0 39.0 12-13 35.78580515665618 38.0 35.0 39.0 30.0 39.0 14-15 36.932712161819 39.0 36.0 41.0 31.0 41.0 16-17 36.846875647588575 39.0 36.0 41.0 30.5 41.0 18-19 36.81818918208414 39.0 36.0 41.0 30.0 41.0 20-21 36.73097437574325 39.0 36.0 41.0 30.0 41.0 22-23 36.56280261177285 39.0 36.0 41.0 30.0 41.0 24-25 36.416028292383785 39.0 36.0 41.0 29.5 41.0 26-27 36.1865614158715 39.0 35.0 40.5 28.5 41.0 28-29 35.926307961196116 39.0 35.0 40.0 27.0 41.0 30-31 35.64826020127488 38.0 34.5 40.0 27.0 41.0 32-33 35.34085956527767 38.0 34.0 40.0 26.5 41.0 34-35 35.01738034550978 38.0 34.0 40.0 25.0 41.0 36-37 34.79694827689882 38.0 34.0 40.0 24.5 41.0 38-39 34.539298097226364 38.0 33.0 40.0 24.0 41.0 40-41 34.23294702060804 38.0 33.0 40.0 23.5 41.0 42-43 33.79979953018303 38.0 33.0 40.0 22.0 41.0 44-45 33.31643966797746 37.5 32.0 40.0 18.5 41.0 46-47 32.82665249695245 37.0 31.0 40.0 16.5 41.0 48-49 32.32782082749209 37.0 30.5 39.5 13.0 41.0 50-51 31.58587304008949 35.5 30.0 38.5 9.0 40.0 52-53 31.568236516615787 36.0 30.0 39.0 8.5 40.0 54-55 31.511004222588088 36.0 30.0 39.0 7.5 40.0 56-57 31.048670582967617 36.0 29.0 39.0 2.0 40.0 58-59 30.55812456858488 35.0 28.0 39.0 2.0 40.0 60-61 29.977600206333832 35.0 28.0 38.5 2.0 40.0 62-63 29.20813155073889 34.0 26.5 38.0 2.0 40.0 64-65 28.506572169879533 34.0 26.0 38.0 2.0 40.0 66-67 27.77602119593117 33.5 24.0 37.0 2.0 39.5 68-69 26.900432992938832 32.5 22.0 36.0 2.0 39.0 70-71 26.041929201455268 32.0 19.0 36.0 2.0 39.0 72-73 25.121672606052954 31.0 14.5 35.0 2.0 38.0 74-75 24.153318163092184 30.5 7.5 35.0 2.0 37.0 76-77 23.179070384584996 30.0 2.0 34.0 2.0 36.5 78-79 22.190224934688285 29.0 2.0 34.0 2.0 36.0 80-81 21.218028816169575 28.5 2.0 34.0 2.0 35.5 82-83 20.201995703366293 26.5 2.0 33.5 2.0 35.0 84-85 19.21552990076396 25.5 2.0 33.0 2.0 35.0 86-87 18.20998641834383 24.0 2.0 32.0 2.0 34.5 88-89 17.119774006807226 19.5 2.0 31.5 2.0 34.0 90-91 15.984212629200908 12.0 2.0 31.0 2.0 34.0 92-93 14.831952970595939 2.0 2.0 30.5 2.0 34.0 94-95 13.600554447632106 2.0 2.0 29.5 2.0 34.0 96-97 12.234645100341753 2.0 2.0 29.0 2.0 34.0 98-99 10.60929829103705 2.0 2.0 26.0 2.0 33.5 100-101 7.519846213709571 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.577970770026976 1101 2 0.4967709580074029 1101 3 0.4404932862503337 1101 4 0.4017848701712694 1101 5 0.37868760334226437 1101 6 0.5097456979236128 1101 7 0.41225986465158115 1101 8 0.5323804231007756 1101 9 0.6980580279496493 1101 10-11 0.6969298096802987 1101 12-13 0.618301290502508 1101 14-15 0.67497166814438 1101 16-17 0.6797326925223786 1101 18-19 0.5977879736439675 1101 20-21 0.8647604318939415 1101 22-23 0.7857099902855609 1101 24-25 0.8128922189837624 1101 26-27 0.8471885434126847 1101 28-29 0.8551257076348335 1101 30-31 0.881732539282666 1101 32-33 1.049064611294476 1101 34-35 1.0667554598177134 1101 36-37 1.207943849557708 1101 38-39 1.1869121420736448 1101 40-41 1.2042369719963801 1101 42-43 1.3102192869033544 1101 44-45 1.3772244246490075 1101 46-47 1.5287322571480857 1101 48-49 1.245348147529775 1101 50-51 1.609738989162139 1101 52-53 1.6535879072947068 1101 54-55 1.6856143340821212 1101 56-57 1.7205758299025682 1101 58-59 1.6557085287976427 1101 60-61 1.6917122106044111 1101 62-63 1.5939885950364001 1101 64-65 1.8777950983454907 1101 66-67 2.132144020992687 1101 68-69 2.1956690870613578 1101 70-71 2.3676617693519155 1101 72-73 2.551530000316152 1101 74-75 2.444113104491656 1101 76-77 2.6798782584307155 1101 78-79 2.8716720172670698 1101 80-81 3.1371117033841998 1101 82-83 3.1966424350833194 1101 84-85 3.205501136222427 1101 86-87 3.1865482046265754 1101 88-89 3.5756916973519175 1101 90-91 3.6460191507318243 1101 92-93 3.396895092801307 1101 94-95 3.5300578114103 1101 96-97 3.3043282890366186 1101 98-99 2.8877753398848487 1101 100-101 1.9901959665776756 1102 1 0.7014587652658903 1102 2 0.6366374244030268 1102 3 0.6417091546470068 1102 4 0.4716964464932829 1102 5 0.44759562206359504 1102 6 0.5415871784023238 1102 7 0.48804008725000614 1102 8 0.6116612288442269 1102 9 0.3283714873675976 1102 10-11 0.6284864961244665 1102 12-13 0.6773754969921342 1102 14-15 0.7418191024286074 1102 16-17 0.6658892993866985 1102 18-19 0.5433132226246258 1102 20-21 0.52363698574419 1102 22-23 0.5357517134191596 1102 24-25 0.6076518423449926 1102 26-27 0.5429014853169463 1102 28-29 0.5435330634355253 1102 30-31 0.5403367303897895 1102 32-33 0.7862651529855569 1102 34-35 0.7179522652694743 1102 36-37 0.8410500671121639 1102 38-39 0.9109254817303025 1102 40-41 1.0750549313950017 1102 42-43 1.1363505466581216 1102 44-45 1.0824033789620486 1102 46-47 1.2634367521366627 1102 48-49 1.2859498954476507 1102 50-51 1.3871707871331012 1102 52-53 1.2833250735769326 1102 54-55 1.3247400619122125 1102 56-57 1.5735044757580816 1102 58-59 1.7656522894394975 1102 60-61 1.725187133410497 1102 62-63 1.3385634864706049 1102 64-65 1.70653105624746 1102 66-67 1.7822615309560703 1102 68-69 2.118014382331449 1102 70-71 1.8134235309931626 1102 72-73 1.9801110075541999 1102 74-75 1.934755131751423 1102 76-77 1.94304600598349 1102 78-79 2.2618537930917313 1102 80-81 2.4814337599700664 1102 82-83 2.575618551831745 1102 84-85 2.8771679717393113 1102 86-87 2.925980383367012 1102 88-89 3.074794711157015 1102 90-91 2.7418252539086083 1102 92-93 3.113234801820992 1102 94-95 2.871536332439005 1102 96-97 2.7016743306736153 1102 98-99 2.3800947349997426 1102 100-101 1.8818313225408518 1103 1 0.45197563542650343 1103 2 0.39912394493012116 1103 3 0.31884979220327736 1103 4 0.29279870569549615 1103 5 0.2531739051282713 1103 6 0.3620448543770749 1103 7 0.31399264679978955 1103 8 0.2881815978930504 1103 9 0.30482681454039096 1103 10-11 0.3820458671999134 1103 12-13 0.4475117931551509 1103 14-15 0.5291325325426328 1103 16-17 0.500964863457817 1103 18-19 0.6318003242735273 1103 20-21 0.6880215133152561 1103 22-23 0.8066700925513004 1103 24-25 0.6970594992934736 1103 26-27 0.6516277951813123 1103 28-29 0.6968817474620934 1103 30-31 0.6185836877065185 1103 32-33 0.7652956521545988 1103 34-35 0.766316310120402 1103 36-37 0.9894673611999139 1103 38-39 0.9195890596170386 1103 40-41 1.1290525752986298 1103 42-43 1.081007204575542 1103 44-45 1.1540467014712803 1103 46-47 1.3690805633717744 1103 48-49 1.199289315565899 1103 50-51 1.156684626463857 1103 52-53 1.1813104774505092 1103 54-55 1.289176866131296 1103 56-57 1.4679987721326349 1103 58-59 1.7126852249434812 1103 60-61 1.7032666838799493 1103 62-63 1.7051846035617864 1103 64-65 1.7663062423462499 1103 66-67 2.2372853776177237 1103 68-69 2.314942825326195 1103 70-71 2.1467778560347703 1103 72-73 2.4389458218566027 1103 74-75 2.3425710984986097 1103 76-77 2.5301454665614287 1103 78-79 3.0204455817676354 1103 80-81 2.904741485850778 1103 82-83 3.169939970547027 1103 84-85 3.2444678342395683 1103 86-87 3.349151825242064 1103 88-89 3.7007858095380186 1103 90-91 3.478395686965925 1103 92-93 3.8003873900621503 1103 94-95 3.4049152153478257 1103 96-97 3.1696980423885677 1103 98-99 2.7446758470820907 1103 100-101 2.0189744888280066 1104 1 0.553796769865663 1104 2 0.49235529567220127 1104 3 0.5257125269630087 1104 4 0.5878377166143309 1104 5 0.4813423779319379 1104 6 0.5944512756246993 1104 7 0.5861333790263643 1104 8 0.5829344857789849 1104 9 0.8655532061680731 1104 10-11 0.7533414702551795 1104 12-13 0.76986407130142 1104 14-15 0.8910423159116334 1104 16-17 1.0322898286883557 1104 18-19 0.8395668891216417 1104 20-21 0.8670429069289227 1104 22-23 1.023395167216286 1104 24-25 0.9639699915258504 1104 26-27 1.1811469749150802 1104 28-29 0.929830068393251 1104 30-31 0.9089474810745983 1104 32-33 0.9807898822411047 1104 34-35 0.9400717276526507 1104 36-37 1.1702352535557878 1104 38-39 1.11427410752097 1104 40-41 1.0009788910017647 1104 42-43 1.1762919157400873 1104 44-45 1.1821838905526718 1104 46-47 1.2817919481861608 1104 48-49 1.3251240461216582 1104 50-51 1.196899324189289 1104 52-53 1.3443790473860417 1104 54-55 1.6195760909511172 1104 56-57 1.6785350594929547 1104 58-59 1.6513091395330655 1104 60-61 1.8650734770172157 1104 62-63 2.2215807890566843 1104 64-65 2.1750517416496855 1104 66-67 2.166926294383103 1104 68-69 2.6403576275650273 1104 70-71 2.646470057014522 1104 72-73 2.7417225744187625 1104 74-75 2.7168525981019975 1104 76-77 2.885576238730394 1104 78-79 2.9368591080524062 1104 80-81 3.0733941416278974 1104 82-83 3.284002999631518 1104 84-85 3.427770762606876 1104 86-87 3.539942342642025 1104 88-89 3.5351388516163844 1104 90-91 3.4353324130013654 1104 92-93 3.3572234692158265 1104 94-95 3.3895681476402384 1104 96-97 3.1246781883558405 1104 98-99 2.648952109298266 1104 100-101 2.049028069525364 1105 1 0.5723175634643489 1105 2 0.46108629548937685 1105 3 0.39842738609340245 1105 4 0.36295275548351924 1105 5 0.5092936398965264 1105 6 0.4462412900516881 1105 7 0.47300624851634865 1105 8 0.4039480362310499 1105 9 0.3990802420645849 1105 10-11 0.5215704009055884 1105 12-13 0.6188184780235488 1105 14-15 0.6405960458831927 1105 16-17 0.616169684763058 1105 18-19 0.6875056776794892 1105 20-21 0.615695593265265 1105 22-23 0.5285813429937605 1105 24-25 0.724997957780964 1105 26-27 0.4647820398231204 1105 28-29 0.5742397376778925 1105 30-31 0.7570217674397597 1105 32-33 0.8622180000389363 1105 34-35 0.8635677187749167 1105 36-37 0.767852080973654 1105 38-39 0.8254975106216875 1105 40-41 0.9493719049093698 1105 42-43 1.1855693196960786 1105 44-45 1.1113085791930288 1105 46-47 1.0858329157412 1105 48-49 1.0887932685611688 1105 50-51 0.9972690955795329 1105 52-53 1.1474074177849047 1105 54-55 1.3928562590176377 1105 56-57 1.6211865915804289 1105 58-59 1.5306962866429537 1105 60-61 1.6625440876435142 1105 62-63 1.4789448319442613 1105 64-65 1.673939898783285 1105 66-67 1.9525400077010744 1105 68-69 2.1832229351361043 1105 70-71 2.052357822759742 1105 72-73 2.0751426218732085 1105 74-75 2.2875943137705335 1105 76-77 2.5834634575612547 1105 78-79 2.634105985057321 1105 80-81 2.7544924996092988 1105 82-83 3.056910017254289 1105 84-85 3.195733173210211 1105 86-87 3.046813233931161 1105 88-89 3.031860722170883 1105 90-91 2.9618189335989342 1105 92-93 2.9746716482976066 1105 94-95 3.0002774646753476 1105 96-97 2.948879126121506 1105 98-99 2.5717043729843834 1105 100-101 2.0157891482893104 1106 1 0.4384569885745222 1106 2 0.26396430977048624 1106 3 0.1996525063393264 1106 4 0.40298674921687194 1106 5 0.26230541425977805 1106 6 -0.001905342906454166 1106 7 0.3515980844052322 1106 8 -0.05642618004806366 1106 9 0.28897249868607844 1106 10-11 0.3644087662294808 1106 12-13 0.47600613831615846 1106 14-15 0.6199916900684457 1106 16-17 0.04398235647530413 1106 18-19 0.49159216739870715 1106 20-21 0.5950456703394096 1106 22-23 0.44972561894015683 1106 24-25 0.631471183705159 1106 26-27 0.749841976728824 1106 28-29 0.6201844207647653 1106 30-31 0.37305167550783125 1106 32-33 0.41035959721853743 1106 34-35 0.38690053070462227 1106 36-37 0.6368878586204119 1106 38-39 0.800682040710015 1106 40-41 1.0534582163709416 1106 42-43 0.3907761810111836 1106 44-45 0.7867685841931618 1106 46-47 1.0352023961602654 1106 48-49 0.8739259568692077 1106 50-51 0.5228768093227103 1106 52-53 0.8640815935549568 1106 54-55 0.7124234227111863 1106 56-57 1.3250019291357908 1106 58-59 1.4655411111327048 1106 60-61 1.5756928396394407 1106 62-63 1.6376676693781853 1106 64-65 1.6218448812182196 1106 66-67 1.8379612282935724 1106 68-69 1.9891036994870674 1106 70-71 1.8319069244971722 1106 72-73 1.9949648945423455 1106 74-75 2.2599473325415076 1106 76-77 2.3876610240769836 1106 78-79 2.7179118125671984 1106 80-81 2.628807043249669 1106 82-83 2.9236122908860125 1106 84-85 3.1239015803399823 1106 86-87 2.585425511515755 1106 88-89 2.493439068857942 1106 90-91 2.612577887814794 1106 92-93 2.8364989095070055 1106 94-95 2.855433099199038 1106 96-97 2.801691341048535 1106 98-99 2.5009710700439793 1106 100-101 1.8016467881669724 1107 1 0.26529618766921104 1107 2 0.18500257512013007 1107 3 0.13789852143164794 1107 4 0.17727862131313543 1107 5 0.12018793980697495 1107 6 0.12356735841454736 1107 7 0.15356679655358363 1107 8 0.3321271975272708 1107 9 0.38729721677127316 1107 10-11 0.33523776350558876 1107 12-13 0.41910427253205995 1107 14-15 0.5198813693992506 1107 16-17 0.5264149272312224 1107 18-19 0.5544564672889578 1107 20-21 0.5548397573790069 1107 22-23 0.531074317554328 1107 24-25 0.5871111857523417 1107 26-27 0.43431775451797705 1107 28-29 0.5550710577472131 1107 30-31 0.6653246793576884 1107 32-33 0.7280319448310095 1107 34-35 0.6242207620368276 1107 36-37 0.7191634783211853 1107 38-39 0.7942760492740888 1107 40-41 0.7855168910443808 1107 42-43 0.8926328665006054 1107 44-45 0.7393463346750977 1107 46-47 0.8201510174362383 1107 48-49 0.8142731826455716 1107 50-51 1.1492323603289591 1107 52-53 1.0895053898370435 1107 54-55 1.0339370905322376 1107 56-57 1.2981667106838124 1107 58-59 1.3778390224326067 1107 60-61 1.4341028003448102 1107 62-63 1.429966263073986 1107 64-65 1.595468858335213 1107 66-67 1.7865725526534 1107 68-69 1.887784969425823 1107 70-71 2.0414095716245697 1107 72-73 2.132289959512118 1107 74-75 2.1628864685145786 1107 76-77 2.3910985933828144 1107 78-79 2.3506918365647884 1107 80-81 2.548739258591066 1107 82-83 2.805947335895709 1107 84-85 2.665721075253291 1107 86-87 2.5294903732452845 1107 88-89 2.6114395351618036 1107 90-91 2.4785866111089447 1107 92-93 2.470520041033124 1107 94-95 2.3856149008379273 1107 96-97 2.154205685578848 1107 98-99 1.7746260393077318 1107 100-101 0.9260489809324381 1108 1 0.3608903352502928 1108 2 0.27108681689299274 1108 3 0.18634538697160252 1108 4 0.1715543208147423 1108 5 0.2014403533947231 1108 6 0.3238620434669883 1108 7 0.16217471316367948 1108 8 0.38300700180936076 1108 9 0.4158237619009739 1108 10-11 0.4216929523015409 1108 12-13 0.3004961825031742 1108 14-15 0.5639102855022031 1108 16-17 0.5617297605863456 1108 18-19 0.4649053301058075 1108 20-21 0.5378713631651095 1108 22-23 0.4803131571034527 1108 24-25 0.4225639293433616 1108 26-27 0.4079812560802196 1108 28-29 0.5115455848532022 1108 30-31 0.6812757185803662 1108 32-33 0.663785464280771 1108 34-35 0.611188868629327 1108 36-37 0.7921055365653586 1108 38-39 0.827853522426949 1108 40-41 0.7446396499766124 1108 42-43 0.8874189882600518 1108 44-45 1.0335605128032697 1108 46-47 0.856696278260209 1108 48-49 1.0721357156850289 1108 50-51 1.0973021034449744 1108 52-53 1.258493859179353 1108 54-55 1.3036111351110158 1108 56-57 1.6375034007281748 1108 58-59 1.8257594561995312 1108 60-61 1.853861079928894 1108 62-63 1.6031284151419598 1108 64-65 1.955821790952708 1108 66-67 2.2033340709391425 1108 68-69 2.157636345292442 1108 70-71 2.373637360166999 1108 72-73 2.2118579902233186 1108 74-75 2.3393583816495322 1108 76-77 2.4280532917419784 1108 78-79 2.544101055423109 1108 80-81 2.6935943883400455 1108 82-83 2.9440831219629047 1108 84-85 2.990196620877448 1108 86-87 2.9786879729600315 1108 88-89 3.173395195783769 1108 90-91 3.091673427516394 1108 92-93 3.1169510611712763 1108 94-95 3.033279943712552 1108 96-97 2.6091167999891454 1108 98-99 2.3586249201220717 1108 100-101 1.634948727529518 1109 1 0.760341834243345 1109 2 0.5117282093584201 1109 3 0.5525365612977744 1109 4 0.48924448819277444 1109 5 0.46751309800824004 1109 6 0.5953454101025244 1109 7 0.5860737700611764 1109 8 0.5633827451972024 1109 9 0.5035479605791338 1109 10-11 0.6383557764068186 1109 12-13 0.6461754685355672 1109 14-15 0.9169722157685811 1109 16-17 0.887916915002144 1109 18-19 0.8020430455355623 1109 20-21 0.763979006118241 1109 22-23 0.8894240179554345 1109 24-25 0.930141903781454 1109 26-27 0.9197020535989111 1109 28-29 0.9634197202768959 1109 30-31 0.7469005092100289 1109 32-33 0.9001389523412442 1109 34-35 1.1050395388114396 1109 36-37 1.035042120508578 1109 38-39 1.2716715801008434 1109 40-41 1.2427826582883625 1109 42-43 1.4529669276618762 1109 44-45 1.5303002472527183 1109 46-47 1.6794135504751395 1109 48-49 1.9440439316724465 1109 50-51 1.8972748606699739 1109 52-53 1.829506610571542 1109 54-55 2.2568853422625246 1109 56-57 2.2296795516245744 1109 58-59 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0.7025003110705512 2316 92-93 0.34966783934259915 2316 94-95 0.10711351754612686 2316 96-97 0.09577996847049519 2316 98-99 -0.055811653405410055 2316 100-101 0.29432386417738243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6959.0 3 2827.0 4 3227.0 5 3886.0 6 4615.0 7 5893.0 8 7503.0 9 9842.0 10 12263.0 11 14965.0 12 18064.0 13 20882.0 14 23850.0 15 27238.0 16 30568.0 17 34567.0 18 35551.0 19 36436.0 20 39645.0 21 43572.0 22 48188.0 23 53168.0 24 58310.0 25 64202.0 26 70448.0 27 77003.0 28 84116.0 29 92622.0 30 101334.0 31 111717.0 32 124014.0 33 138281.0 34 153936.0 35 164227.0 36 156877.0 37 106392.0 38 24784.0 39 301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75729980996172 18.06910328772714 14.32002566439808 23.853571237913062 2 15.843863912817191 19.305297410474125 39.86104158661338 24.989797090095298 3 19.52334293289109 23.048632622289965 29.078606508112397 28.34941793670655 4 13.230266860496334 15.915526989322796 35.9573587989985 34.89684735118237 5 14.52112014201925 36.49884758539927 33.765623484891805 15.214408787689676 6 33.65996497498426 34.93909406334934 16.94859715961244 14.452343802053965 7 29.814842618214072 31.63420131863237 19.893332166695334 18.657623896458226 8 26.913458842339505 34.77311731519173 19.129517419395896 19.18390642307287 9 26.41121384748694 16.796709740379505 19.540515025257108 37.251561386876446 10-11 25.62871167394864 25.96364010580298 26.89546897064815 21.51217924960023 12-13 26.41534430001002 23.060543402533106 27.206216620820978 23.317895676635896 14-15 22.98993560407129 24.795005009455952 25.291004528269813 26.924054858202943 16-17 23.074634655532357 27.90083507306889 26.027636942041955 22.996893329356798 18-19 23.06158782266481 26.43673561400295 28.002397966500546 22.499278596831694 20-21 23.440410505023188 25.779751249148052 28.28373942555346 22.496098820275297 22-23 23.211076194072014 25.641090011475974 28.05432608526524 23.093507709186774 24-25 23.043123763311492 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27.11720221910244 23.325502405817005 50-51 23.2865965229324 26.53558391689113 27.086425698859077 23.091393861317393 52-53 23.458851770950595 26.390953527612314 27.000032305279927 23.15016239615716 54-55 23.177920592738968 26.39384160593773 27.219428820779264 23.208808980544042 56-57 23.129459968012977 26.631879868751817 27.136709233196125 23.101950930039074 58-59 23.23484450151925 26.20665509046434 27.099470522926943 23.45902988508946 60-61 22.98622415927165 26.418020490435534 27.360215733785857 23.235539616506955 62-63 23.03505720518993 26.492318756627387 27.33269665705191 23.13992738113077 64-65 22.821054096399166 26.41033446357728 27.258775335749547 23.50983610427401 66-67 22.810771858984314 26.484609411399283 27.14764404410623 23.556974685510173 68-69 23.08579812034494 26.13290082199188 27.32929796342125 23.45200309424193 70-71 22.95812712088699 26.239244128614438 27.03495184476008 23.767676905738494 72-73 23.088017816729923 26.229603117033122 26.973449603300924 23.70892946293603 74-75 22.980885192118492 26.49587368754835 26.877876608484037 23.645364511849124 76-77 23.20272027162958 26.21183687912245 26.885121004774987 23.70032184447298 78-79 23.217905399108922 26.32590870019758 26.834068606681647 23.622117294011844 80-81 23.30499018642561 26.42990165550949 26.66665506963438 23.598453088430517 82-83 23.354657407402804 26.4252843113232 26.55423771861069 23.665820562663303 84-85 23.416515827883757 26.166726803380776 26.620808694675464 23.795948674060003 86-87 23.50196909726165 26.17374919647066 26.481171384888256 23.843110321379434 88-89 23.424624818364297 26.250574387570513 26.348714204377842 23.97608658968734 90-91 23.37457210538442 26.336809512364795 26.25379677637189 24.034821605878893 92-93 23.521743225347603 25.852660520501452 26.512960350450204 24.112635903700745 94-95 23.36727415518668 25.726488504275945 26.49650692029913 24.40973042023824 96-97 23.61096441250626 25.6020945661318 26.33347789186442 24.45346312949752 98-99 23.519018382954105 25.599631615281503 26.006857760855095 24.874492240909294 100-101 24.58580806890888 24.597202654192003 26.138681059338175 24.678308217560947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 222.0 1 189.0 2 197.0 3 267.0 4 234.5 5 197.0 6 330.5 7 723.0 8 1137.5 9 1314.5 10 1208.5 11 1031.5 12 1009.5 13 1089.5 14 1242.5 15 1390.5 16 1524.5 17 1681.0 18 1876.5 19 2137.0 20 2449.0 21 2903.0 22 3571.5 23 4390.5 24 5548.0 25 7008.0 26 8767.5 27 11132.5 28 13313.0 29 15753.5 30 18942.5 31 22764.5 32 27204.5 33 30215.5 34 32294.0 35 34995.0 36 38514.5 37 42076.0 38 45452.0 39 48542.5 40 51820.0 41 56270.5 42 60313.5 43 63671.5 44 66843.0 45 70739.0 46 73653.5 47 75456.5 48 76806.5 49 76411.5 50 75546.5 51 74728.0 52 74081.0 53 74225.5 54 74840.5 55 72498.0 56 67775.5 57 62915.5 58 58034.0 59 51909.5 60 43742.0 61 36719.5 62 30898.0 63 24796.5 64 19331.5 65 15116.0 66 11653.0 67 8992.5 68 6965.0 69 5251.5 70 4092.5 71 3078.0 72 2246.0 73 1742.0 74 1307.5 75 994.5 76 722.5 77 488.5 78 331.5 79 201.5 80 113.0 81 65.5 82 45.5 83 36.5 84 24.0 85 11.0 86 8.5 87 5.5 88 4.5 89 5.0 90 3.5 91 1.0 92 2.5 93 3.5 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39457866800379476 2 0.02941946743806631 3 0.035879823463317355 4 0.04398011601805521 5 0.0635102692328526 6 0.056602657790468795 7 0.03950756184672755 8 0.04159475379334713 9 0.03776823522454458 10-11 0.03968149450894585 12-13 0.03148181186151183 14-15 0.028698889266019073 16-17 0.023505756922644193 18-19 0.02825163384888631 20-21 0.034885922536355654 22-23 0.03376778399352374 24-25 0.04238987453491649 26-27 0.037320979807411815 28-29 0.03289812068243225 30-31 0.034885922536355654 32-33 0.03046306341137609 34-35 0.031407269291989705 36-37 0.03319629096052076 38-39 0.015902414831387192 40-41 0.01376552783841954 42-43 0.014610343626336982 44-45 0.012622541772413584 46-47 0.02186582039315739 48-49 0.018809575042750163 50-51 0.012995254620024222 52-53 0.010833520103882525 54-55 0.009442058806136145 56-57 0.01028687459405359 58-59 0.004919809588460413 60-61 0.008125140077911895 62-63 0.012995254620024222 64-65 0.008473005402348488 66-67 0.004248926462761265 68-69 0.0073797143826906195 70-71 0.014436410964118684 72-73 0.011504403229581671 74-75 0.012249828924802947 76-77 0.006013100608118283 78-79 0.010411112209923802 80-81 0.013119492235894432 82-83 0.015206684182514002 84-85 0.00949175385248423 86-87 0.0028077701186668014 88-89 0.017269028605959528 90-91 0.026512307226703336 92-93 0.011554098275929757 94-95 0.021766430300461218 96-97 0.019902866062408032 98-99 0.012224981401628905 100-101 0.12654843552539838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2012273.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.66300347771493 #Duplication Level Percentage of deduplicated Percentage of total 1 81.04591645347367 49.9753462812514 2 11.065704164786643 13.646891087932067 3 3.363566641440521 6.2222286452601825 4 1.4629978026956838 3.6085135438205294 5 0.8070154123991884 2.4881497090670353 6 0.49311136268753025 1.8244036603381155 7 0.33024479820948477 1.4254720298342107 8 0.23474356762912293 1.1579994741668653 9 0.16921426894673253 0.9390834049087221 >10 0.9313992632341963 10.311921528713215 >50 0.06212336139386177 2.622986218233134 >100 0.02999305973149044 3.27957173270886 >500 0.0027545851968894157 1.164312898878685 >1k 0.0012152581750982665 1.3331197848870509 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4452 0.2212423463416743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3748 0.1862570337126225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2509 0.12468487128734522 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.9695046348084975E-5 0.0 10-11 0.0 0.0 0.0 9.939009269616995E-5 0.0 12-13 0.0 0.0 0.0 1.2423761587021244E-4 7.454256952212746E-5 14-15 0.0 0.0 0.0 1.4908513904425492E-4 9.939009269616995E-5 16-17 0.0 0.0 0.0 1.4908513904425492E-4 9.939009269616995E-5 18-19 0.0 0.0 0.0 1.4908513904425492E-4 9.939009269616995E-5 20-21 0.0 0.0 0.0 1.987801853923399E-4 9.939009269616995E-5 22-23 0.0 0.0 0.0 2.7332275491446736E-4 1.2423761587021244E-4 24-25 0.0 0.0 0.0 4.224078939587223E-4 1.4908513904425492E-4 26-27 0.0 0.0 0.0 7.951207415693596E-4 1.4908513904425492E-4 28-29 0.0 0.0 0.0 0.0024350572710561636 1.739326622182974E-4 30-31 0.0 0.0 0.0 0.008025749985215724 1.987801853923399E-4 32-33 0.0 0.0 0.0 0.021070699651588032 1.987801853923399E-4 34-35 0.0 0.0 0.0 0.04129658351525861 1.987801853923399E-4 36-37 0.0 0.0 0.0 0.07953692168011 1.987801853923399E-4 38-39 0.0 0.0 0.0 0.13571717157662005 2.9817027808850985E-4 40-41 0.0 0.0 0.0 0.1942579361746642 2.9817027808850985E-4 42-43 0.0 0.0 0.0 0.25605372630850787 2.9817027808850985E-4 44-45 0.0 0.0 0.0 0.3356651905581399 3.2301780126255233E-4 46-47 0.0 0.0 0.0 0.43073181422202655 3.478653244365948E-4 48-49 0.0 0.0 0.0 0.5285565129582318 4.4725541713276477E-4 50-51 0.0 0.0 0.0 0.6359723556396175 4.969504634808497E-4 52-53 0.0 0.0 0.0 0.7420464320696049 5.217979866548922E-4 54-55 0.0 0.0 0.0 0.862656309556407 5.466455098289347E-4 56-57 0.0 0.0 0.0 1.0074925221379007 5.466455098289347E-4 58-59 0.0 0.0 0.0 1.1639325280416724 5.466455098289347E-4 60-61 0.0 0.0 0.0 1.3340635192143413 5.714930330029773E-4 62-63 0.0 0.0 0.0 1.502976981751482 5.963405561770197E-4 64-65 0.0 0.0 0.0 1.6829724396242458 5.963405561770197E-4 66-67 0.0 0.0 0.0 1.8884863037967512 5.963405561770197E-4 68-69 0.0 0.0 0.0 2.1155181230379774 5.963405561770197E-4 70-71 0.0 4.9695046348084975E-5 0.0 2.358551747203287 5.963405561770197E-4 72-73 0.0 4.9695046348084975E-5 0.0 2.604293751394567 6.211880793510621E-4 74-75 0.0 4.9695046348084975E-5 0.0 2.855054955267004 6.708831256991472E-4 76-77 0.0 4.9695046348084975E-5 0.0 3.1239051560101436 6.957306488731896E-4 78-79 0.0 4.9695046348084975E-5 0.0 3.4289085029715154 6.957306488731896E-4 80-81 0.0 4.9695046348084975E-5 0.0 3.7443229621428107 6.957306488731896E-4 82-83 0.0 4.9695046348084975E-5 0.0 4.076012548993104 6.957306488731896E-4 84-85 0.0 4.9695046348084975E-5 0.0 4.404695585539338 6.957306488731896E-4 86-87 0.0 4.9695046348084975E-5 0.0 4.741901322534268 6.957306488731896E-4 88-89 0.0 4.9695046348084975E-5 0.0 5.100152911657613 6.957306488731896E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2860 0.0 51.700573 1 GTATCAA 4795 0.0 44.663982 1 ATCAACG 6300 0.0 33.72665 3 TATCAAC 6360 0.0 33.408478 2 TCAACGC 6490 0.0 32.592793 4 CAACGCA 6635 0.0 31.73644 5 AACGCAG 6940 0.0 30.40337 6 ACGCAGA 7935 0.0 26.162638 7 TAAGGTG 1040 0.0 26.051754 5 CGCAGAG 8050 0.0 25.67086 8 GTGGTAT 1180 0.0 23.848974 1 GTAAGGT 990 0.0 23.52703 4 GCAGAGT 8860 0.0 22.677734 9 GTACATG 6130 0.0 22.176043 1 TACATGG 5885 0.0 22.131474 2 ACATGGG 6090 0.0 20.84012 3 GAGTACT 5255 0.0 20.608915 12-13 AGAGTAC 8230 0.0 20.228815 10-11 CAGAGTA 8455 0.0 20.055655 10-11 TGGTATC 1345 0.0 19.437778 2 >>END_MODULE