FastQCFastQC Report
Thu 26 May 2016
SRR938632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012273
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46010.228646908247539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39250.19505305691623354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26810.1332324192592158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.053.723331
GTATCAA48100.041.0919231
GTGGTAT12000.033.258781
ATCAACG63550.030.7928283
TCAACGC64500.030.3392934
TATCAAC64850.030.2510472
CAACGCA65700.029.785155
AACGCAG68800.028.4430856
TGGTATC12550.028.3868942
ACGCAGA78900.024.7418847
CGCAGAG79800.024.462848
GGACCGA6000.022.1653586
TAAGGTG9700.021.0554565
GCAGAGT90950.020.993819
GTACATG61800.020.6041431
TACATGG60550.020.5535772
GTAAGGT9150.020.2447034
AGGACCG7550.020.131265
GAGTACT54250.020.0932812-13
CAGAGTA85400.019.88319410-11
Produced by FastQC (version 0.11.5)