##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2012273 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78361385358746 31.0 31.0 34.0 30.0 34.0 2 32.04528659878655 31.0 31.0 34.0 30.0 34.0 3 32.10454148120061 33.0 31.0 34.0 30.0 34.0 4 35.75837672125005 37.0 35.0 37.0 35.0 37.0 5 35.64457655596433 37.0 35.0 37.0 33.0 37.0 6 35.67763071909229 37.0 35.0 37.0 33.0 37.0 7 35.60269804345633 37.0 35.0 37.0 33.0 37.0 8 35.57195022742938 37.0 35.0 37.0 33.0 37.0 9 37.11229341148045 39.0 37.0 39.0 33.0 39.0 10-11 37.03974411026735 39.0 37.0 39.0 33.0 39.0 12-13 36.914681556627755 39.0 37.0 39.0 33.0 39.0 14-15 37.92606992192411 40.0 37.0 41.0 33.0 41.0 16-17 38.01066505389676 40.0 37.0 41.0 33.0 41.0 18-19 38.132740438300374 40.0 37.0 41.0 33.0 41.0 20-21 38.08329163090694 40.0 37.0 41.0 33.0 41.0 22-23 37.95122431200936 40.0 37.0 41.0 33.0 41.0 24-25 37.81981917960436 40.0 37.0 41.0 32.0 41.0 26-27 37.59113947262623 39.5 37.0 41.0 32.0 41.0 28-29 37.43901746929964 39.0 36.5 41.0 31.5 41.0 30-31 37.181682356221046 39.0 36.0 41.0 31.0 41.0 32-33 37.08854091865268 39.0 36.0 41.0 31.0 41.0 34-35 36.94947330705128 39.0 36.0 41.0 30.5 41.0 36-37 36.81716968820831 39.0 35.5 41.0 30.0 41.0 38-39 36.589074394975235 39.0 35.0 40.0 30.0 41.0 40-41 36.35521174313823 39.0 35.0 40.0 30.0 41.0 42-43 36.082173243888874 38.0 35.0 40.0 29.5 41.0 44-45 35.78910465925846 38.0 35.0 40.0 28.5 41.0 46-47 35.36645673822588 38.0 34.0 40.0 27.0 41.0 48-49 34.9461479630249 38.0 34.0 40.0 26.0 41.0 50-51 34.52654013645265 38.0 33.0 40.0 24.5 41.0 52-53 33.88689780164023 37.0 33.0 40.0 23.5 41.0 54-55 33.41820120828535 37.0 32.0 39.5 22.5 41.0 56-57 32.978205740473584 36.0 31.0 39.0 21.0 40.0 58-59 32.39399574511013 36.0 31.0 39.0 19.0 40.0 60-61 31.848135665488726 35.0 30.0 39.0 17.0 40.0 62-63 31.275846766318487 35.0 29.5 38.0 12.0 40.0 64-65 30.56870265615053 34.0 28.5 38.0 8.0 40.0 66-67 29.869323645449697 34.0 28.0 37.5 6.5 39.5 68-69 29.08808422117675 34.0 26.5 36.5 2.0 39.0 70-71 28.265747242049166 33.0 26.0 36.0 2.0 39.0 72-73 27.653016514160853 32.0 25.5 35.5 2.0 38.0 74-75 26.905855964871566 32.0 23.5 35.0 2.0 37.5 76-77 25.786532443659482 30.5 22.5 34.0 2.0 36.0 78-79 26.250565653865056 31.5 23.5 34.5 2.0 36.0 80-81 25.84432430390906 31.0 21.5 34.5 2.0 36.0 82-83 25.12485085274215 31.0 19.5 34.0 2.0 35.5 84-85 24.312018548179097 31.0 16.0 34.0 2.0 35.0 86-87 23.36640281910059 30.0 7.0 34.0 2.0 35.0 88-89 22.334783848911158 29.0 2.0 34.0 2.0 35.0 90-91 21.432627680240206 29.0 2.0 34.0 2.0 35.0 92-93 20.307175765912476 27.0 2.0 33.0 2.0 34.5 94-95 19.09227599833621 25.5 2.0 32.5 2.0 34.0 96-97 17.700054863331168 22.5 2.0 32.0 2.0 34.0 98-99 15.94591091765382 9.5 2.0 32.0 2.0 34.0 100-101 11.918967008949581 2.0 2.0 26.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3078154802449653 1101 2 0.20398421734483208 1101 3 0.33211353685946676 1101 4 0.2736030186661367 1101 5 0.19997066526853757 1101 6 0.229437662082546 1101 7 0.24196722036448648 1101 8 0.27215090166686906 1101 9 0.3190988500236216 1101 10-11 0.371510653057058 1101 12-13 0.336899276867328 1101 14-15 0.5043422778905295 1101 16-17 0.4355344837542816 1101 18-19 0.4605808378917118 1101 20-21 0.39495090955336565 1101 22-23 0.36877812687507827 1101 24-25 0.4029279632232132 1101 26-27 0.552653960990078 1101 28-29 0.46611141143761614 1101 30-31 0.5151728214118663 1101 32-33 0.525102550100641 1101 34-35 0.3371285821348735 1101 36-37 0.43044534809285295 1101 38-39 0.5519662418745739 1101 40-41 0.7264925492772889 1101 42-43 0.5540429643445108 1101 44-45 0.6389441011296739 1101 46-47 0.8816479265039803 1101 48-49 0.7880918679782454 1101 50-51 0.8222054052641354 1101 52-53 0.9595409313796921 1101 54-55 1.0531978676880271 1101 56-57 1.171439518558607 1101 58-59 1.1101078973546947 1101 60-61 1.1994697647156407 1101 62-63 1.2510952124149952 1101 64-65 1.3895753103843305 1101 66-67 1.4127823487589026 1101 68-69 1.518276069659354 1101 70-71 1.7737067055349485 1101 72-73 2.001200667395821 1101 74-75 2.229773814424803 1101 76-77 2.0398160392807334 1101 78-79 2.013517593194223 1101 80-81 2.2523530663681903 1101 82-83 2.5111910427632473 1101 84-85 2.7736989349401995 1101 86-87 2.4575930922732603 1101 88-89 2.280047859648711 1101 90-91 2.7957947186861745 1101 92-93 3.1358787672930966 1101 94-95 3.050385819348641 1101 96-97 2.938359072015732 1101 98-99 3.0337249713358787 1101 100-101 2.3983348811113903 1102 1 0.22028328246605966 1102 2 0.17689403517746882 1102 3 0.1992293940484018 1102 4 0.21226462863583606 1102 5 0.1756479137049567 1102 6 0.2709559730322155 1102 7 0.3116578231401874 1102 8 0.266138365639371 1102 9 0.31634096312781423 1102 10-11 0.3754213709347738 1102 12-13 0.4582518062680663 1102 14-15 0.4986204578907376 1102 16-17 0.3657422037820268 1102 18-19 0.36661997394632806 1102 20-21 0.3424902479835268 1102 22-23 0.3763559533665486 1102 24-25 0.22911220279279831 1102 26-27 0.2986411988501132 1102 28-29 0.32281422673359117 1102 30-31 0.3265022795103363 1102 32-33 0.4227408983833385 1102 34-35 0.28853140932376675 1102 36-37 0.36079500018798427 1102 38-39 0.5708389244053365 1102 40-41 0.48209825416332563 1102 42-43 0.5130402583362326 1102 44-45 0.46077543101845464 1102 46-47 0.3857285957968841 1102 48-49 0.5489707162337751 1102 50-51 0.7281208401807007 1102 52-53 0.726166927167732 1102 54-55 0.8993041082452606 1102 56-57 1.0114457390985194 1102 58-59 0.9886347256966701 1102 60-61 1.1294694808515011 1102 62-63 1.258774960813497 1102 64-65 1.2900925548858098 1102 66-67 1.5462695805011748 1102 68-69 1.5685482855762025 1102 70-71 1.6589190236722509 1102 72-73 1.5302151302734899 1102 74-75 1.3478412282414496 1102 76-77 1.4578381571910448 1102 78-79 1.802364644751293 1102 80-81 1.807320174835123 1102 82-83 1.8234707115818942 1102 84-85 1.7724941029853198 1102 86-87 1.685354906771959 1102 88-89 1.6049402726945203 1102 90-91 2.1026840278244947 1102 92-93 2.0683029523216128 1102 94-95 1.7233706654086305 1102 96-97 2.014857886882968 1102 98-99 2.176961467845576 1102 100-101 1.7287814874911884 1103 1 0.17500635604118386 1103 2 0.23530625964610152 1103 3 0.24428311134358438 1103 4 0.08762931904258409 1103 5 0.09826631727338508 1103 6 0.21724572911909945 1103 7 0.19037940379150342 1103 8 0.2583756481290038 1103 9 0.35716173081647185 1103 10-11 0.32593508018178596 1103 12-13 0.33749141202919475 1103 14-15 0.36592919526380285 1103 16-17 0.27639569835437783 1103 18-19 0.3451674612023723 1103 20-21 0.26733369158005615 1103 22-23 0.4102561438723811 1103 24-25 0.3307714392862735 1103 26-27 0.4800138134241152 1103 28-29 0.4635791921398251 1103 30-31 0.45322743928844034 1103 32-33 0.41003998281254894 1103 34-35 0.3937838751403149 1103 36-37 0.3542407242621124 1103 38-39 0.3621175990585783 1103 40-41 0.4947831220189016 1103 42-43 0.4875879741506495 1103 44-45 0.6790057570666974 1103 46-47 0.7070968580360031 1103 48-49 0.6062392978467415 1103 50-51 0.5732539450367113 1103 52-53 0.7133174486576976 1103 54-55 0.7835422125724278 1103 56-57 1.0716933602071066 1103 58-59 1.1924001032374036 1103 60-61 0.8931392772278848 1103 62-63 1.1509625197007054 1103 64-65 1.1705140991866223 1103 66-67 0.9053572678916879 1103 68-69 1.1539999375790693 1103 70-71 1.4028549932885745 1103 72-73 1.428081756739818 1103 74-75 1.4342037096558862 1103 76-77 1.5859118113408996 1103 78-79 1.7140686568016683 1103 80-81 1.8512961421658645 1103 82-83 2.167690433980738 1103 84-85 2.0452736523606134 1103 86-87 2.1207448601728025 1103 88-89 2.033169054758037 1103 90-91 2.1083731860124146 1103 92-93 2.283996402057614 1103 94-95 2.5448178289141836 1103 96-97 2.195187886262943 1103 98-99 2.500677073154449 1103 100-101 2.029134050931331 1104 1 0.22707770167476227 1104 2 0.1912013666141732 1104 3 0.2076089811072208 1104 4 0.20272389078952102 1104 5 0.17027160387095108 1104 6 0.17834658807403514 1104 7 0.1778980187779311 1104 8 0.1745591746129591 1104 9 0.20323175714401032 1104 10-11 0.33824071394663946 1104 12-13 0.3202041677033236 1104 14-15 0.37381527096103184 1104 16-17 0.3257418451254068 1104 18-19 0.4792350832528314 1104 20-21 0.40435709813136356 1104 22-23 0.4600470584701597 1104 24-25 0.39440335055766695 1104 26-27 0.4412969850077175 1104 28-29 0.4025781598941549 1104 30-31 0.4587767263827942 1104 32-33 0.6865080622849291 1104 34-35 0.4497258491080416 1104 36-37 0.6079615539486056 1104 38-39 0.5061260632387103 1104 40-41 0.6135050823717663 1104 42-43 0.7271403240034289 1104 44-45 0.7373781364691183 1104 46-47 0.8074812846006765 1104 48-49 0.7004850847963908 1104 50-51 0.7971066701769871 1104 52-53 0.7679170345877537 1104 54-55 0.8299964311296506 1104 56-57 0.9006036204478391 1104 58-59 0.9943692202279806 1104 60-61 1.0415265251859473 1104 62-63 1.017638911269799 1104 64-65 1.1235279401832976 1104 66-67 1.0995600543691495 1104 68-69 1.433864468516564 1104 70-71 1.4708659226728962 1104 72-73 1.747091363303074 1104 74-75 1.6312672577923983 1104 76-77 1.5850222756874182 1104 78-79 1.7481423715620785 1104 80-81 1.9089389195511295 1104 82-83 2.1364696306569293 1104 84-85 2.390352441645412 1104 86-87 2.282105713808331 1104 88-89 2.2320900592584394 1104 90-91 2.504308293964691 1104 92-93 2.635308502296752 1104 94-95 2.6168245215217354 1104 96-97 2.717897525281593 1104 98-99 2.830543303020683 1104 100-101 2.2724672788139166 1105 1 0.1254505419616727 1105 2 0.11748451742267463 1105 3 0.13357526173381018 1105 4 0.06673891795509235 1105 5 0.16150732373353804 1105 6 0.08723813097447675 1105 7 0.13681790320983112 1105 8 0.12409625547326186 1105 9 0.23628897033788832 1105 10-11 0.21595545335560473 1105 12-13 0.22327780846786993 1105 14-15 0.27743190995749956 1105 16-17 0.30952395480501593 1105 18-19 0.30034843886105733 1105 20-21 0.28561570380857404 1105 22-23 0.26961725405470105 1105 24-25 0.3424868789698081 1105 26-27 0.3753744485782775 1105 28-29 0.25930405398387535 1105 30-31 0.3460245365435384 1105 32-33 0.41184393684551424 1105 34-35 0.3936384842394176 1105 36-37 0.41364829957771576 1105 38-39 0.3814151569295703 1105 40-41 0.4634117779774485 1105 42-43 0.49489003325937375 1105 44-45 0.2647527215560359 1105 46-47 0.20477014242913327 1105 48-49 0.32129665288533005 1105 50-51 0.4647651226107712 1105 52-53 0.41412845547527155 1105 54-55 0.5283749553873349 1105 56-57 0.711031110994881 1105 58-59 0.6141921354470838 1105 60-61 0.5400575320297598 1105 62-63 0.6216892551388398 1105 64-65 0.6748670635583593 1105 66-67 1.0123746559977604 1105 68-69 1.184199968248361 1105 70-71 1.263365856098833 1105 72-73 1.579536184590303 1105 74-75 1.6221001765762857 1105 76-77 1.3991898861393857 1105 78-79 1.4685489679220538 1105 80-81 1.7994439358791219 1105 82-83 1.8550315099656416 1105 84-85 1.7520711669948241 1105 86-87 2.0195722785215686 1105 88-89 1.6781122531503208 1105 90-91 1.9235098661420587 1105 92-93 2.154277971541436 1105 94-95 2.000804048485577 1105 96-97 2.2474405228421865 1105 98-99 2.4262990775657585 1105 100-101 2.073016968019319 1106 1 0.032799347167507875 1106 2 0.09284351718336126 1106 3 -0.00504523798476697 1106 4 0.06944843419503144 1106 5 0.08654597221971017 1106 6 -0.0750801632067919 1106 7 -0.004450391038290036 1106 8 0.019402642489332322 1106 9 0.030015237003311768 1106 10-11 0.0629165838299528 1106 12-13 0.1423167501878737 1106 14-15 0.16880526480653657 1106 16-17 0.14503905033105724 1106 18-19 0.13730073666898335 1106 20-21 -0.012963738717367335 1106 22-23 0.2579319082148146 1106 24-25 0.156802015316849 1106 26-27 -0.08826600485570424 1106 28-29 0.17465996988726573 1106 30-31 0.22289640895741059 1106 32-33 0.20972906583497064 1106 34-35 0.12062831865142698 1106 36-37 0.20970539639345276 1106 38-39 0.3523451226194325 1106 40-41 0.24019298409543666 1106 42-43 0.2761623293916742 1106 44-45 0.2553382667325437 1106 46-47 -0.19895546468298875 1106 48-49 0.04552928713672344 1106 50-51 0.24037829549439493 1106 52-53 0.2233132253201333 1106 54-55 0.02049139952161738 1106 56-57 0.2767025652163113 1106 58-59 0.48559294643024487 1106 60-61 0.7101669109221866 1106 62-63 0.6111404132119347 1106 64-65 0.7115556068947981 1106 66-67 0.950376646244397 1106 68-69 0.8789539125330457 1106 70-71 0.7610362514698359 1106 72-73 1.236902815560878 1106 74-75 1.1088828176683272 1106 76-77 1.1240483161440729 1106 78-79 1.3514421608418417 1106 80-81 1.415726189929245 1106 82-83 1.7320620316856683 1106 84-85 1.7841628912498528 1106 86-87 1.7172053066332502 1106 88-89 1.895652983908633 1106 90-91 2.0922065531791176 1106 92-93 2.2391269405214835 1106 94-95 2.053425037521393 1106 96-97 1.938480246221971 1106 98-99 1.846687652498364 1106 100-101 1.6718687436660264 1107 1 0.06897770270714076 1107 2 0.12735568942008513 1107 3 0.08949218289995997 1107 4 0.15926310684403688 1107 5 0.010788654945429244 1107 6 0.02475435578940477 1107 7 0.047650535314133435 1107 8 0.095066130328469 1107 9 0.07427145391421419 1107 10-11 0.0771161050154987 1107 12-13 0.16197259519301355 1107 14-15 0.27035146016510225 1107 16-17 0.0840740856734783 1107 18-19 0.18229479413808747 1107 20-21 0.176123897975053 1107 22-23 0.16131460151766674 1107 24-25 0.28338218830420914 1107 26-27 0.24708466133328955 1107 28-29 0.1305231722574547 1107 30-31 0.1923591927914572 1107 32-33 0.36944240269401973 1107 34-35 0.3721438061948561 1107 36-37 0.37758473742648135 1107 38-39 0.36421312450739407 1107 40-41 0.4767042919640261 1107 42-43 0.39955372324214977 1107 44-45 0.366605760235565 1107 46-47 0.5217640649187771 1107 48-49 0.6028377471398016 1107 50-51 0.655043267544599 1107 52-53 0.8057779255493074 1107 54-55 0.7141254988443393 1107 56-57 0.7141695116652969 1107 58-59 0.7833435989713209 1107 60-61 0.8135257203688191 1107 62-63 1.1573158685690288 1107 64-65 1.3041965358630705 1107 66-67 1.1102136673588632 1107 68-69 1.2946822848482427 1107 70-71 1.2227623718785914 1107 72-73 1.4804285845357477 1107 74-75 1.3856824946676056 1107 76-77 1.409884052079608 1107 78-79 1.531608892904778 1107 80-81 1.7665444963363761 1107 82-83 2.0241047269219763 1107 84-85 2.288120787423317 1107 86-87 2.5229144012644973 1107 88-89 2.5847335123105175 1107 90-91 2.6581757543778615 1107 92-93 2.526960106099164 1107 94-95 2.235667390422428 1107 96-97 2.421541189861756 1107 98-99 2.218127109766165 1107 100-101 1.8920906929298908 1108 1 0.03912915652761839 1108 2 0.06619003598442674 1108 3 0.08196707630027333 1108 4 0.05621195126158085 1108 5 0.015572052760944644 1108 6 0.08860800048137207 1108 7 0.12866396934879987 1108 8 0.1094366264627098 1108 9 0.1627607182336419 1108 10-11 0.1286477101671366 1108 12-13 0.16103148102381226 1108 14-15 0.2040449970159628 1108 16-17 0.13127868051007852 1108 18-19 0.050620021200387555 1108 20-21 0.07795712493076934 1108 22-23 0.1258457003710305 1108 24-25 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-0.1282487929085434 2316 54-55 0.06815669718690742 2316 56-57 0.04508233359608482 2316 58-59 -0.07346836263106127 2316 60-61 0.1486389327275397 2316 62-63 -0.0036830349448493394 2316 64-65 -0.034852306179782744 2316 66-67 0.16851653105094755 2316 68-69 0.45684824042737304 2316 70-71 0.21704320747679162 2316 72-73 0.6111674398255005 2316 74-75 0.835347710799887 2316 76-77 0.7895063149354016 2316 78-79 0.4486720914051041 2316 80-81 0.11572680659653045 2316 82-83 0.7876792539695607 2316 84-85 0.9568605639475258 2316 86-87 1.1817500211779652 2316 88-89 0.9437653653543485 2316 90-91 1.309393687032916 2316 92-93 1.2854849035461164 2316 94-95 1.504769988866343 2316 96-97 1.4020752931503502 2316 98-99 1.3733523458297228 2316 100-101 0.8413566631539453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 43.0 5 81.0 6 169.0 7 389.0 8 1022.0 9 1815.0 10 2983.0 11 4448.0 12 6685.0 13 9288.0 14 12713.0 15 16614.0 16 20751.0 17 25343.0 18 26309.0 19 26508.0 20 28002.0 21 30579.0 22 33091.0 23 37087.0 24 41367.0 25 46756.0 26 52935.0 27 61498.0 28 71725.0 29 83252.0 30 95865.0 31 110831.0 32 128181.0 33 151203.0 34 179872.0 35 213075.0 36 236927.0 37 195881.0 38 57922.0 39 1049.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37350648653458 18.944090888530223 15.019363420408663 23.66303920452653 2 16.343106328350167 20.04491956177831 38.77793742771644 24.834036682155087 3 19.623899852402555 23.337441544952615 29.554225908569098 27.48443269407573 4 13.743463237840988 16.698678559022557 35.585430008751295 33.97242819438516 5 14.404130658410649 36.56060071461583 33.94882396497488 15.086444661998637 6 33.56398460845024 35.51993193766452 16.601524743412053 14.314558710473182 7 29.670427422124135 31.706731641283266 19.886665477298557 18.736175459294042 8 26.906601095676923 35.12835242949263 18.93312633679741 19.03192013803303 9 26.09324621421444 16.674402563480736 19.57128885673053 37.6610623655743 10-11 25.591482865396493 26.132140122140484 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36-37 23.29823424510456 25.913210325428356 27.784468721790052 23.004086707677033 38-39 22.78112059824351 26.159759068420424 27.85901854526053 23.200101788075536 40-41 23.201932775803126 25.945358129867447 27.915161241503288 22.93754785282614 42-43 23.08286193156899 25.969145289481894 27.7345257426985 23.21346703625062 44-45 22.976838468606676 26.238570681010742 27.76898997594246 23.01560087444012 46-47 23.27728223484809 26.462539091404686 27.171514174780633 23.08866449896659 48-49 23.11985004022814 26.404990774114644 27.24508553262902 23.230073653028192 50-51 23.20535036747002 26.56237498589903 27.182345536614566 23.049929110016386 52-53 23.449825147979425 26.424371842190396 27.011816985071114 23.11398602475907 54-55 23.107724448919207 26.45110777712567 27.19375054975145 23.247417224203673 56-57 23.07358395207807 26.636346062388156 27.186445377938284 23.103624607595492 58-59 23.172675874496154 26.264428335519085 27.098336060763128 23.46455972922163 60-61 22.905440762759326 26.50366028863877 27.349072417112392 23.241826531489515 62-63 22.916721538280342 26.59470161354846 27.300917917201094 23.187658930970102 64-65 22.69865174754003 26.460296237361607 27.25807625859632 23.58297575650204 66-67 22.656111007854875 26.557656240216193 27.199796844732326 23.586435907196606 68-69 22.99760491569994 26.198380764980833 27.261446348645656 23.542567970673574 70-71 22.80773245558564 26.37059661183891 26.995462590410884 23.826208342164566 72-73 22.924431536952333 26.40759083780093 26.91239437291653 23.755583252330208 74-75 22.86300119317806 26.655105942384555 26.69366929835067 23.788223566086707 76-77 22.852117978027835 26.40635738788922 27.064270106491517 23.677254527591433 78-79 22.877611536804398 26.51111454559098 27.030328389835773 23.580945527768847 80-81 22.905709922480543 26.64549322183068 26.85438676439383 23.59441009129495 82-83 22.892376364052453 26.61390976313313 26.851479824683683 23.642234048130735 84-85 22.833184652123244 26.4567983758545 26.889373724924027 23.820643247098232 86-87 22.9483335921727 26.443460164621836 26.739878863177513 23.868327380027953 88-89 22.74509969571723 26.701794438428582 26.49068988154192 24.062415984312267 90-91 22.756252090324896 26.603468659662553 26.676968582882715 23.963310667129832 92-93 22.78058570477615 26.25700114721784 26.781834052715368 24.180579095290643 94-95 22.539113728604416 26.159273617446537 26.82856650166255 24.473046152286493 96-97 22.649690834616088 26.08329637263343 26.79224607999165 24.474766712758832 98-99 22.496301446175544 26.136090878325156 26.511561801008117 24.856045874491183 100-101 22.77943897274376 25.75083003151163 26.698613955462307 24.77111704028231 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 276.0 1 207.0 2 188.5 3 256.0 4 226.0 5 203.5 6 357.5 7 759.0 8 1214.0 9 1435.5 10 1296.0 11 1083.0 12 1075.5 13 1157.0 14 1286.5 15 1451.0 16 1629.5 17 1784.0 18 1980.0 19 2243.0 20 2519.0 21 2961.5 22 3654.5 23 4539.5 24 5642.5 25 7108.0 26 9100.5 27 11503.0 28 13625.0 29 16138.0 30 19426.5 31 23113.5 32 27870.0 33 31050.0 34 32526.5 35 35120.0 36 38872.0 37 42596.0 38 46001.0 39 49192.5 40 52679.0 41 57326.0 42 61414.5 43 64630.5 44 68084.5 45 72430.5 46 75098.5 47 76422.5 48 77673.5 49 77419.5 50 76696.0 51 75525.0 52 74276.0 53 74655.0 54 75630.0 55 72604.5 56 66984.0 57 61737.0 58 57015.5 59 50556.5 60 41566.0 61 34592.0 62 29332.5 63 22823.0 64 17067.0 65 13345.0 66 10241.0 67 8140.0 68 6270.5 69 4476.5 70 3474.0 71 2561.0 72 1858.5 73 1494.0 74 1150.0 75 912.0 76 651.0 77 387.5 78 235.5 79 133.5 80 77.5 81 48.0 82 24.0 83 11.5 84 5.0 85 2.5 86 2.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024350572710561638 2 0.011529250752755715 3 0.002136886992967654 4 0.0 5 1.4908513904425492E-4 6 0.0 7 0.0 8 4.9695046348084975E-5 9 4.4725541713276477E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016647840526608467 26-27 0.008522700448696574 28-29 1.739326622182974E-4 30-31 0.0 32-33 2.9817027808850985E-4 34-35 0.009740229084224655 36-37 8.696633110914871E-4 38-39 0.012398914063847202 40-41 0.012150438832106777 42-43 0.004994352157982541 44-45 6.957306488731896E-4 46-47 9.939009269616995E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.454256952212746E-5 66-67 9.442058806136146E-4 68-69 0.0013169187282242518 70-71 7.454256952212746E-5 72-73 2.4847523174042487E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.987801853923399E-4 82-83 0.0012175286355280818 84-85 0.006857916396035726 86-87 0.004248926462761265 88-89 0.0 90-91 0.0012920712050502093 92-93 6.708831256991471E-4 94-95 0.0 96-97 2.4847523174042487E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2012273.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.594988851058694 #Duplication Level Percentage of deduplicated Percentage of total 1 78.89124878199665 46.22631842827191 2 12.230794543743492 14.33326539860479 3 3.7334641814773035 6.562868762684689 4 1.6594471528671093 3.8894114968467743 5 0.8986856563531395 2.632923800730929 6 0.5577709544251747 1.9609549713592487 7 0.36274824680658185 1.4878660634160927 8 0.26834603649169514 1.2578986413165332 9 0.20917437981329562 1.1030913407778458 >10 1.0777795361213731 11.380351097912035 >50 0.07134900682232141 2.8587772761492714 >100 0.03519741192011037 3.7515577234217083 >500 0.002719394833570779 1.1727140226072776 >1k 0.0012747163282363 1.3820009759009104 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4601 0.228646908247539 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3925 0.19505305691623354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2681 0.1332324192592158 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.9695046348084975E-5 9 0.0 0.0 0.0 0.0 4.9695046348084975E-5 10-11 0.0 0.0 0.0 4.9695046348084975E-5 4.9695046348084975E-5 12-13 0.0 0.0 0.0 4.9695046348084975E-5 1.2423761587021244E-4 14-15 0.0 0.0 0.0 4.9695046348084975E-5 1.4908513904425492E-4 16-17 0.0 0.0 0.0 4.9695046348084975E-5 1.4908513904425492E-4 18-19 0.0 0.0 0.0 4.9695046348084975E-5 1.4908513904425492E-4 20-21 0.0 0.0 0.0 1.2423761587021244E-4 1.4908513904425492E-4 22-23 0.0 0.0 0.0 2.2362770856638239E-4 1.987801853923399E-4 24-25 0.0 0.0 0.0 3.727128476106373E-4 2.4847523174042487E-4 26-27 0.0 0.0 0.0 7.205781720472321E-4 2.9817027808850985E-4 28-29 0.0 0.0 0.0 0.0023605147015340363 2.9817027808850985E-4 30-31 0.0 0.0 0.0 0.007976054938867638 2.9817027808850985E-4 32-33 0.0 0.0 0.0 0.021021004605239944 2.9817027808850985E-4 34-35 0.0 0.0 0.0 0.04154505874699904 2.9817027808850985E-4 36-37 0.0 0.0 0.0 0.08013326223628703 2.9817027808850985E-4 38-39 0.0 0.0 0.0 0.13676076754992986 2.9817027808850985E-4 40-41 0.0 0.0 0.0 0.19535122719432205 2.9817027808850985E-4 42-43 0.0 0.0 0.0 0.2572215598976878 2.9817027808850985E-4 44-45 0.0 0.0 0.0 0.3365348538692315 2.9817027808850985E-4 46-47 0.0 0.0 0.0 0.43237175075151335 2.9817027808850985E-4 48-49 0.0 0.0 0.0 0.5309170276597659 2.9817027808850985E-4 50-51 0.0 0.0 0.0 0.6381589376789332 3.478653244365948E-4 52-53 0.0 0.0 0.0 0.7441336240162244 3.478653244365948E-4 54-55 0.0 0.0 0.0 0.8646441114103305 3.478653244365948E-4 56-57 0.0 0.0 0.0 1.0087845933429511 3.727128476106373E-4 58-59 0.0 0.0 0.0 1.1652742942930705 3.975603707846798E-4 60-61 0.0 0.0 0.0 1.3356040656511317 3.975603707846798E-4 62-63 0.0 0.0 0.0 1.5036230173540073 4.4725541713276477E-4 64-65 0.0 0.0 0.0 1.6825251842071132 4.7210294030680726E-4 66-67 0.0 0.0 0.0 1.8889335592138838 4.969504634808497E-4 68-69 0.0 0.0 0.0 2.11830104563347 4.969504634808497E-4 70-71 0.0 0.0 0.0 2.364937560659016 5.466455098289347E-4 72-73 0.0 0.0 0.0 2.6147297111276653 5.466455098289347E-4 74-75 0.0 0.0 0.0 2.871404625515524 5.466455098289347E-4 76-77 0.0 0.0 0.0 3.149125392031797 5.466455098289347E-4 78-79 0.0 0.0 0.0 3.4658319224081424 5.466455098289347E-4 80-81 0.0 0.0 0.0 3.7951112995105536 5.466455098289347E-4 82-83 0.0 0.0 0.0 4.143498421933803 5.466455098289347E-4 84-85 0.0 0.0 0.0 4.492457037390055 5.466455098289347E-4 86-87 0.0 0.0 0.0 4.853789719386982 5.714930330029773E-4 88-89 0.0 0.0 0.0 5.244243698543885 5.963405561770197E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3060 0.0 53.72333 1 GTATCAA 4810 0.0 41.091923 1 GTGGTAT 1200 0.0 33.25878 1 ATCAACG 6355 0.0 30.792828 3 TCAACGC 6450 0.0 30.339293 4 TATCAAC 6485 0.0 30.251047 2 CAACGCA 6570 0.0 29.78515 5 AACGCAG 6880 0.0 28.443085 6 TGGTATC 1255 0.0 28.386894 2 ACGCAGA 7890 0.0 24.741884 7 CGCAGAG 7980 0.0 24.46284 8 GGACCGA 600 0.0 22.165358 6 TAAGGTG 970 0.0 21.055456 5 GCAGAGT 9095 0.0 20.99381 9 GTACATG 6180 0.0 20.604143 1 TACATGG 6055 0.0 20.553577 2 GTAAGGT 915 0.0 20.244703 4 AGGACCG 755 0.0 20.13126 5 GAGTACT 5425 0.0 20.09328 12-13 CAGAGTA 8540 0.0 19.883194 10-11 >>END_MODULE