FastQCFastQC Report
Thu 26 May 2016
SRR938631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066983
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.24654290819034314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40090.19395418346449875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27140.13130248289415056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.054.465981
GTATCAA49200.043.8941841
ATCAACG63700.033.303563
TCAACGC64500.033.0379834
TATCAAC65050.032.8317642
CAACGCA65850.032.2884335
AACGCAG68300.031.1734056
ACGCAGA79600.026.6260437
CGCAGAG80450.026.225958
TGGTATC11850.025.288172
GTACATG54250.024.814351
TACATGG53300.024.4522342
TAAGGTG11450.023.2636435
ACATGGG54650.023.238943
GCAGAGT90450.022.53849
GTGGTAT15050.021.8086531
AGAGTAC81400.021.45343210-11
CATGGGG39350.020.7911684
GAGTACT60350.020.19297412-13
GTAAGGT10850.020.1661644
Produced by FastQC (version 0.11.5)