##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938631_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2066983 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.314482025251298 31.0 31.0 34.0 30.0 34.0 2 31.363872852365017 31.0 31.0 34.0 28.0 34.0 3 31.41308854499529 31.0 31.0 34.0 28.0 34.0 4 35.08472396725082 37.0 35.0 37.0 32.0 37.0 5 35.057073038336554 37.0 35.0 37.0 32.0 37.0 6 35.08234658920755 37.0 35.0 37.0 32.0 37.0 7 35.0000246736427 37.0 35.0 37.0 32.0 37.0 8 34.96748787967777 37.0 35.0 37.0 32.0 37.0 9 36.495712349835486 39.0 37.0 39.0 32.0 39.0 10-11 36.32916526163979 39.0 35.0 39.0 32.0 39.0 12-13 36.19280226300845 39.0 35.0 39.0 32.0 39.0 14-15 37.18265559029755 39.0 36.0 41.0 31.5 41.0 16-17 36.991566210268786 39.0 36.0 41.0 31.0 41.0 18-19 37.14507037551833 39.0 36.0 41.0 31.0 41.0 20-21 37.12517688824727 39.0 36.0 41.0 31.0 41.0 22-23 37.03126126339694 39.0 36.0 41.0 31.0 41.0 24-25 36.8973482123462 39.0 36.0 41.0 30.5 41.0 26-27 36.68367132192186 39.0 36.0 41.0 30.0 41.0 28-29 36.472020089183125 39.0 36.0 40.5 30.0 41.0 30-31 36.21610216436226 39.0 35.0 40.0 29.5 41.0 32-33 35.94160498659157 38.0 35.0 40.0 29.0 41.0 34-35 35.68806782639238 38.0 35.0 40.0 27.5 41.0 36-37 35.40408798717745 38.0 34.0 40.0 26.5 41.0 38-39 35.26158318670255 38.0 34.0 40.0 26.0 41.0 40-41 35.15101962618948 38.0 34.0 40.0 25.5 41.0 42-43 34.85132001569437 38.0 33.5 40.0 24.5 41.0 44-45 34.509515075837584 38.0 33.0 40.0 24.0 41.0 46-47 34.12621777731118 38.0 33.0 40.0 23.5 41.0 48-49 33.76232218649113 38.0 33.0 40.0 22.5 41.0 50-51 33.081915526155754 37.0 31.5 39.5 20.5 40.5 52-53 33.116659885446566 37.0 32.0 39.0 20.0 40.0 54-55 33.21014444724509 37.0 32.0 40.0 19.5 41.0 56-57 32.87037242202766 37.0 31.5 40.0 17.5 41.0 58-59 32.47437400307598 36.5 31.0 39.0 14.0 41.0 60-61 31.975871596428224 36.0 31.0 39.0 10.0 40.5 62-63 31.391676903003074 35.0 30.0 39.0 7.5 40.0 64-65 30.85360789130825 35.0 29.0 38.0 4.5 40.0 66-67 30.284245927518512 34.5 28.5 38.0 2.0 40.0 68-69 29.659543402146994 34.0 28.0 37.5 2.0 39.5 70-71 28.9903697804965 34.0 26.5 37.0 2.0 39.0 72-73 28.312953468896453 33.5 26.0 36.0 2.0 39.0 74-75 27.60023764104494 33.0 25.5 35.5 2.0 38.5 76-77 26.816810539806085 32.5 23.5 35.0 2.0 37.5 78-79 26.002088067487733 32.0 21.0 35.0 2.0 37.0 80-81 25.27135830338227 31.0 19.0 35.0 2.0 36.0 82-83 24.499253985156145 31.0 15.5 34.0 2.0 36.0 84-85 23.75386928678175 30.0 7.5 34.0 2.0 35.0 86-87 22.933921807774908 29.5 3.5 34.0 2.0 35.0 88-89 22.063218952453887 29.0 2.0 34.0 2.0 35.0 90-91 21.088934451807297 28.5 2.0 34.0 2.0 35.0 92-93 20.08342521443089 26.5 2.0 33.0 2.0 35.0 94-95 19.012688541705472 25.5 2.0 33.0 2.0 34.5 96-97 17.71064928932652 22.0 2.0 32.0 2.0 34.0 98-99 16.128311650361905 10.0 2.0 32.0 2.0 34.0 100-101 12.652936429569086 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.351215351135 1101 2 0.29834616011783766 1101 3 0.3178471070497224 1101 4 0.2360919176991345 1101 5 0.1839442081150935 1101 6 0.38305015929615394 1101 7 0.25131614232097377 1101 8 0.334744801833196 1101 9 0.445314071414721 1101 10-11 0.4617642937127613 1101 12-13 0.5324256217030623 1101 14-15 0.6404605024278922 1101 16-17 0.525022926074918 1101 18-19 0.5866389290315652 1101 20-21 0.8126293862861402 1101 22-23 0.674602494491964 1101 24-25 0.5921092904456771 1101 26-27 0.5291989798945806 1101 28-29 0.5978971881519186 1101 30-31 0.5217315942988066 1101 32-33 0.6986422267120176 1101 34-35 0.522315118711937 1101 36-37 0.7405637259997278 1101 38-39 0.5490283420104376 1101 40-41 0.5998417537478815 1101 42-43 0.6479144854490642 1101 44-45 0.6523237370428703 1101 46-47 0.42274716629498954 1101 48-49 0.3088492780848071 1101 50-51 0.4648478401925189 1101 52-53 0.5196089230724041 1101 54-55 0.4514023501558668 1101 56-57 0.5143604893620832 1101 58-59 0.5082063776783698 1101 60-61 0.23633680557259495 1101 62-63 0.08754070631792743 1101 64-65 -0.05568536769389709 1101 66-67 0.289831371779929 1101 68-69 0.4065298746524135 1101 70-71 0.4884194897655476 1101 72-73 0.4758908039199561 1101 74-75 0.42068549029690416 1101 76-77 0.46519301950372594 1101 78-79 0.6370126335271316 1101 80-81 0.5200639398997637 1101 82-83 0.5039825008163952 1101 84-85 0.7246745072486434 1101 86-87 0.8969519874350134 1101 88-89 0.8103382787529689 1101 90-91 0.47856985763530346 1101 92-93 0.2912116182890294 1101 94-95 0.1485609355978852 1101 96-97 -0.17490237284313181 1101 98-99 -0.010765581196018559 1101 100-101 0.3835077305172998 1102 1 0.3555069902712731 1102 2 0.20181297319965807 1102 3 0.2067592610586111 1102 4 0.2087042184333825 1102 5 0.2055481985484775 1102 6 0.20574047573281007 1102 7 0.28391522846706607 1102 8 0.3368851928733321 1102 9 0.5323550893722881 1102 10-11 0.49579806225894174 1102 12-13 0.4981312838128744 1102 14-15 0.5734367937196012 1102 16-17 0.6687083072041133 1102 18-19 0.5745550257027858 1102 20-21 0.4737589543924443 1102 22-23 0.5944231359268315 1102 24-25 0.6316770555362083 1102 26-27 0.7078495098740092 1102 28-29 0.4982301895695471 1102 30-31 0.7064535450946394 1102 32-33 0.7429017910028435 1102 34-35 0.4220098803375194 1102 36-37 0.7047897264774363 1102 38-39 0.7859207734015996 1102 40-41 0.8776590822296555 1102 42-43 0.895123446552887 1102 44-45 0.909293966995314 1102 46-47 1.0092135357885255 1102 48-49 1.0365892112673762 1102 50-51 0.8266545783916683 1102 52-53 0.8795024929034412 1102 54-55 0.8619355556861379 1102 56-57 1.068895481653577 1102 58-59 1.1129257848210656 1102 60-61 0.9785406638600982 1102 62-63 0.9776890137028964 1102 64-65 1.0426865392083968 1102 66-67 0.7005855959502156 1102 68-69 0.8023447897657441 1102 70-71 0.8930573138874429 1102 72-73 0.9580666199828158 1102 74-75 1.082905417027284 1102 76-77 0.9889684211372476 1102 78-79 1.0915223443882205 1102 80-81 1.1825258238781657 1102 82-83 1.042043461634833 1102 84-85 0.9976534662741123 1102 86-87 1.185410006301474 1102 88-89 1.08413927201849 1102 90-91 1.1380693163335103 1102 92-93 0.9707950795805225 1102 94-95 1.021040935349724 1102 96-97 1.0761259454609338 1102 98-99 1.2149254704184251 1102 100-101 1.2715792381901352 1103 1 0.27381276240500796 1103 2 0.19859447855343504 1103 3 0.30804597644523923 1103 4 0.18950201950904244 1103 5 0.2254714716493993 1103 6 0.32411415381680087 1103 7 0.290536058617306 1103 8 0.31354721446588485 1103 9 0.37255067727701174 1103 10-11 0.4224029055058125 1103 12-13 0.4954342403236325 1103 14-15 0.5834701964517919 1103 16-17 0.5620910600311433 1103 18-19 0.6077449119816478 1103 20-21 0.45669310566830745 1103 22-23 0.6155341233399199 1103 24-25 0.6745388477329115 1103 26-27 0.5654437581717744 1103 28-29 0.7048030120897479 1103 30-31 0.8654215026141756 1103 32-33 0.7134164472993518 1103 34-35 0.815423344398333 1103 36-37 0.9421758336536712 1103 38-39 0.6929588284591617 1103 40-41 0.6598945742641433 1103 42-43 0.7782906619695851 1103 44-45 0.8761543063800374 1103 46-47 0.5591799667038231 1103 48-49 0.7576406743377575 1103 50-51 0.9148926450512533 1103 52-53 0.8393393437394039 1103 54-55 0.934260724523277 1103 56-57 0.8255168449849606 1103 58-59 0.8935008441427996 1103 60-61 1.0813887469032686 1103 62-63 0.9194236183106916 1103 64-65 1.0801814366648692 1103 66-67 1.2518886053525442 1103 68-69 1.0714194517608888 1103 70-71 0.9058404384057148 1103 72-73 1.191133106818146 1103 74-75 1.4620019010602192 1103 76-77 1.3702117057207666 1103 78-79 1.307689441651977 1103 80-81 1.5795621471633794 1103 82-83 1.4469920864327044 1103 84-85 1.6477981289815915 1103 86-87 1.722885815873056 1103 88-89 1.622215870100998 1103 90-91 1.5562390206397794 1103 92-93 1.6826200562591787 1103 94-95 1.483702579876578 1103 96-97 1.4550606623353488 1103 98-99 1.5467137514381584 1103 100-101 1.3209603428476235 1104 1 0.24503249744384092 1104 2 0.15014554005724534 1104 3 0.1816473619344201 1104 4 0.11863888429429892 1104 5 0.2784932517393628 1104 6 0.21573189880588473 1104 7 0.16073054075208404 1104 8 0.3058220213360556 1104 9 0.4716622459753168 1104 10-11 0.5008821402139603 1104 12-13 0.5248895221602297 1104 14-15 0.637795418218694 1104 16-17 0.5335323915189818 1104 18-19 0.5308088976722019 1104 20-21 0.5909328252976636 1104 22-23 0.5866152185491131 1104 24-25 0.5214293381769934 1104 26-27 0.5783508990321096 1104 28-29 0.6203224909858065 1104 30-31 0.6561651348413378 1104 32-33 0.7486275548497687 1104 34-35 0.5782265053864464 1104 36-37 0.7804706546605544 1104 38-39 0.8536617096675556 1104 40-41 0.8058482231053645 1104 42-43 0.8887718330897698 1104 44-45 0.8426932425807152 1104 46-47 0.7217772134531728 1104 48-49 0.5555594859444142 1104 50-51 0.8390930227879778 1104 52-53 0.8180035830726595 1104 54-55 0.9458348775134695 1104 56-57 0.9563667071406101 1104 58-59 1.0257825602766175 1104 60-61 0.9921262880690698 1104 62-63 0.941453714517646 1104 64-65 0.946299680530057 1104 66-67 0.9653291686393537 1104 68-69 1.210250195256748 1104 70-71 1.3319920012856983 1104 72-73 1.2792421277254853 1104 74-75 1.128928801505456 1104 76-77 1.1031448017754393 1104 78-79 1.2535949074704398 1104 80-81 1.3308775209568147 1104 82-83 1.4544672733123747 1104 84-85 1.4585308973008004 1104 86-87 1.6381836983201197 1104 88-89 1.4142356705960104 1104 90-91 1.2750081262310573 1104 92-93 1.3171268280193829 1104 94-95 1.5127158052072076 1104 96-97 1.6867255448665048 1104 98-99 1.659671120826939 1104 100-101 1.3718570698112522 1105 1 0.3637069264630952 1105 2 0.21281695682027646 1105 3 0.27224898882014514 1105 4 0.1678464960340733 1105 5 0.16656520723282853 1105 6 0.39340822830928346 1105 7 0.4002539269558625 1105 8 0.2525705892889505 1105 9 0.4582073529527477 1105 10-11 0.48735372318579806 1105 12-13 0.6229538356131101 1105 14-15 0.6867958259427311 1105 16-17 0.6405566076528189 1105 18-19 0.6405657560137215 1105 20-21 0.5962399217009988 1105 22-23 0.6588035380354285 1105 24-25 0.6644402130458147 1105 26-27 0.6270538687335474 1105 28-29 0.5990829653716077 1105 30-31 0.7266702001997274 1105 32-33 0.6940270687001302 1105 34-35 0.8107729090599776 1105 36-37 0.8799007322172443 1105 38-39 0.7785513679591674 1105 40-41 0.8745864843294697 1105 42-43 0.831603576744989 1105 44-45 0.7140563108043096 1105 46-47 0.6805436143194186 1105 48-49 0.6481005869775416 1105 50-51 0.5793861830502536 1105 52-53 0.721629207748208 1105 54-55 0.8738849540452733 1105 56-57 0.6513646239604469 1105 58-59 0.6966579192631457 1105 60-61 0.6894413250650153 1105 62-63 0.7877250448342572 1105 64-65 0.9200716625367278 1105 66-67 0.7433056097655601 1105 68-69 0.6517640768118049 1105 70-71 0.8151293820041374 1105 72-73 0.7740341773296819 1105 74-75 0.8066730850140189 1105 76-77 0.8872426733668775 1105 78-79 0.9867283891885812 1105 80-81 1.007827522405016 1105 82-83 0.9024579513596684 1105 84-85 1.0822183267010423 1105 86-87 1.3025755768139007 1105 88-89 1.2412537374493873 1105 90-91 1.1952434921689452 1105 92-93 1.3585219571654115 1105 94-95 1.2095406821659012 1105 96-97 1.2994055394301824 1105 98-99 1.2979441533356884 1105 100-101 0.9593684604621178 1106 1 0.13054218920070326 1106 2 0.07500066686774076 1106 3 0.02417942363095804 1106 4 -0.004776918702326327 1106 5 0.1895980430222579 1106 6 0.0091081182477879 1106 7 0.33922118969265824 1106 8 0.1367578807443124 1106 9 -0.024076476765557686 1106 10-11 -0.10678858881917819 1106 12-13 -0.029021240090905565 1106 14-15 0.3182383207888577 1106 16-17 -0.06402244919984668 1106 18-19 -0.11323353471911446 1106 20-21 0.1875273046478938 1106 22-23 0.20010974435623297 1106 24-25 -0.004017934697699843 1106 26-27 0.11770421680884624 1106 28-29 0.36053516890245163 1106 30-31 -0.07100214821331718 1106 32-33 0.13061540972331187 1106 34-35 -0.008022974814501538 1106 36-37 -0.13722255753682333 1106 38-39 -0.033546760103433826 1106 40-41 0.3386070799590044 1106 42-43 -0.14566757937914332 1106 44-45 0.05596318244065657 1106 46-47 -0.040041189490743534 1106 48-49 0.1372853068174109 1106 50-51 0.13706517691507258 1106 52-53 0.01571558686291752 1106 54-55 0.04981710637492398 1106 56-57 -0.8512151006810598 1106 58-59 -0.45909602224188006 1106 60-61 0.14640817301858178 1106 62-63 -0.015529037060492357 1106 64-65 -0.07248433674723387 1106 66-67 -0.256540924493482 1106 68-69 0.15848261233101368 1106 70-71 -0.23497971652756178 1106 72-73 0.0683334217389806 1106 74-75 0.505079960603581 1106 76-77 0.4502417791299145 1106 78-79 0.5437267209328596 1106 80-81 0.7367530980079771 1106 82-83 0.7033514585019027 1106 84-85 0.5437527570542571 1106 86-87 0.4880916342567154 1106 88-89 0.46324051263545485 1106 90-91 0.7428921719885224 1106 92-93 0.914746675610516 1106 94-95 0.8673596197572415 1106 96-97 0.7725679899111242 1106 98-99 0.8853074152591347 1106 100-101 0.6298753859837944 1107 1 0.307447094341196 1107 2 0.06675612110426599 1107 3 0.24910546567662095 1107 4 0.0733031083872362 1107 5 0.014975417712001615 1107 6 0.14655163662510517 1107 7 0.2388521103816359 1107 8 0.15078157370682987 1107 9 0.3152583479347797 1107 10-11 0.47028458542700236 1107 12-13 0.48090909874078847 1107 14-15 0.47234074079799626 1107 16-17 0.657080313831699 1107 18-19 0.4861971361278634 1107 20-21 0.45913851735821964 1107 22-23 0.5243517828940156 1107 24-25 0.5821862601022119 1107 26-27 0.5392250309047029 1107 28-29 0.6878246834236705 1107 30-31 0.5938174207952258 1107 32-33 0.7378252245672527 1107 34-35 0.6959940097983193 1107 36-37 0.7395554453202351 1107 38-39 0.6547269165485119 1107 40-41 0.6517633304718373 1107 42-43 0.8391276618552794 1107 44-45 0.8879456398660537 1107 46-47 0.8999822216890649 1107 48-49 0.8508403759242285 1107 50-51 0.7593527681892525 1107 52-53 0.839436687624648 1107 54-55 0.8428211902228853 1107 56-57 0.7960791048818265 1107 58-59 0.6517631588511463 1107 60-61 0.6355297194292682 1107 62-63 0.8632529651897691 1107 64-65 1.0305588764722167 1107 66-67 0.945692303531569 1107 68-69 1.0387391706834492 1107 70-71 1.3754196664323501 1107 72-73 1.368049357551758 1107 74-75 1.2029090329741052 1107 76-77 1.5795819432923537 1107 78-79 1.6562341707633301 1107 80-81 1.5737960366493908 1107 82-83 1.3505673132621325 1107 84-85 1.4070257032325593 1107 86-87 1.5247269886204862 1107 88-89 1.604258548730062 1107 90-91 1.7214385614674619 1107 92-93 1.7791616622878763 1107 94-95 1.6744493367297295 1107 96-97 1.7072993697378038 1107 98-99 1.8026951494659933 1107 100-101 1.5219265919857676 1108 1 0.27627344584455216 1108 2 0.11010677167793403 1108 3 0.24358330641032921 1108 4 0.14350071327164216 1108 5 0.06611882643549905 1108 6 0.15472893168262658 1108 7 0.20988889512990738 1108 8 0.2856860149773226 1108 9 0.28613294704851455 1108 10-11 0.3397931629543578 1108 12-13 0.3093127735533656 1108 14-15 0.46264230144233665 1108 16-17 0.43135191721980704 1108 18-19 0.4405366482106885 1108 20-21 0.38184790686009507 1108 22-23 0.28778310774138305 1108 24-25 0.45406876445768063 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-1.7247986729966946 2316 80-81 -1.7375049472813373 2316 82-83 -1.9095581542954427 2316 84-85 -1.9327907555027721 2316 86-87 -2.1121856518682733 2316 88-89 -2.3268428228902813 2316 90-91 -2.2173492706069453 2316 92-93 -2.0981832131393716 2316 94-95 -2.6325096298329314 2316 96-97 -2.736976449805791 2316 98-99 -2.803361570516854 2316 100-101 -2.610625647772265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6521.0 3 2101.0 4 2293.0 5 2604.0 6 3079.0 7 3866.0 8 4940.0 9 6286.0 10 7960.0 11 10140.0 12 12465.0 13 14894.0 14 17205.0 15 20004.0 16 22844.0 17 25900.0 18 25610.0 19 25467.0 20 26932.0 21 29264.0 22 32875.0 23 36669.0 24 41418.0 25 46048.0 26 52417.0 27 60021.0 28 68295.0 29 78444.0 30 90487.0 31 105069.0 32 123027.0 33 146551.0 34 174927.0 35 207752.0 36 233893.0 37 210452.0 38 84738.0 39 3525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41325274623191 18.260216602026677 14.35472258993474 23.97180806180667 2 15.624795685062235 19.303408953873618 40.314563883560226 24.75723147750393 3 19.58075963885212 22.986827237110216 29.302216997741493 28.13019612629617 4 13.141338458535415 15.872659136119935 36.23652200531927 34.74948040002538 5 14.265349198257935 36.72013551989673 34.14182684387107 14.872688437974258 6 33.666159171426855 35.08758166896202 16.890584413697724 14.355674745913404 7 29.401679291273464 31.81589640676575 20.165819246085228 18.61660505587556 8 26.6057540943227 35.0728468360611 19.291890136702445 19.029508932913743 9 26.09636940443677 16.76017203568463 19.758747437756103 37.384711122122496 10-11 25.30639746285475 26.101506139566222 27.182730993676497 21.409365403902527 12-13 26.07811526986795 23.236000045500564 27.51963603421933 23.166248650412154 14-15 22.7385492327522 24.931833397896725 25.494998776884643 26.83461859246643 16-17 22.693056599737652 28.005532478022165 26.336134335844935 22.965276586395245 18-19 22.695423151249585 26.699107125140408 28.320863865743064 22.284605857866943 20-21 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26.519398764929452 28.11939741020797 22.649322361056985 46-47 23.01099565738064 26.693004632933746 27.47218425286383 22.823815456821784 48-49 22.819380830074092 26.707197618752133 27.519292360638868 22.954129190534907 50-51 22.93378942962965 26.92623674591098 27.474447398533492 22.66552642592588 52-53 23.102629998183172 26.646727519530906 27.49410498461744 22.756537497668482 54-55 22.730107626772572 26.788924951566983 27.648477916286847 22.832489505373594 56-57 22.780916851442583 27.00779143486513 27.452681035035447 22.75861067865684 58-59 22.69352289341552 26.695440043220554 27.52189032325652 23.08914674010741 60-61 22.503049244481783 26.838722852287844 27.683292801750596 22.974935101479776 62-63 22.561075772593433 26.79991571334117 27.830491884264063 22.808516629801336 64-65 22.463948503979942 26.76762948334878 27.635299101195304 23.133122911475976 66-67 22.416316182278493 26.87118746167049 27.55374597956002 23.158750376490993 68-69 22.815093583722145 26.538338529068945 27.70325422557734 22.94331366163157 70-71 22.64113689140602 26.63545737056478 27.419996839871242 23.303408898157954 72-73 22.669018565970532 26.659456111187733 27.423895974429147 23.247629348412588 74-75 22.642205200186893 26.960910159738656 27.25949613495699 23.13738850511746 76-77 22.73180386255087 26.74930196321334 27.312086561400623 23.206807612835167 78-79 22.827603624386033 26.873879958662496 27.264588115079285 23.033928301872187 80-81 22.953184827088375 26.832580674190353 27.041517368573327 23.17271713014794 82-83 22.905529188608995 26.889245680620004 27.13652702519962 23.06869810557138 84-85 22.87868118161645 26.8781625256032 27.11378330774324 23.129372985037115 86-87 22.94549488141305 26.846918989274133 26.95931863460801 23.248267494704805 88-89 23.016266721774738 26.878566557757246 26.77925587648694 23.32591084398108 90-91 22.91218852002229 26.888988423423783 26.893852226060833 23.30497083049309 92-93 23.098264586813126 26.455124394144296 27.004123847540317 23.44248717150226 94-95 22.897821407810717 26.39355894483168 27.137801969837263 23.570817677520342 96-97 23.15817290694289 26.12830868464759 27.05954041230133 23.653977996108193 98-99 22.956869777970912 26.283991277947198 26.80635145846621 23.95278748561568 100-101 23.740540634753334 25.625696115599304 27.011379197905377 23.622384051741978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 232.0 1 168.5 2 167.5 3 235.5 4 204.0 5 202.0 6 367.5 7 771.5 8 1248.5 9 1448.5 10 1300.0 11 1128.0 12 1131.5 13 1217.5 14 1369.0 15 1519.5 16 1615.5 17 1798.0 18 2058.5 19 2368.5 20 2730.5 21 3270.0 22 4030.5 23 5042.0 24 6374.0 25 8233.0 26 10834.5 27 13600.5 28 16071.0 29 18791.0 30 22013.5 31 26448.0 32 32143.5 33 35175.5 34 36160.5 35 38919.5 36 43363.5 37 47991.0 38 51534.0 39 54069.0 40 57201.5 41 61294.5 42 63827.5 43 65966.0 44 69165.5 45 73003.0 46 75570.0 47 77017.0 48 78038.5 49 77976.0 50 77438.0 51 75784.0 52 74408.5 53 74670.5 54 74695.5 55 70950.0 56 65253.0 57 59718.5 58 55040.5 59 48952.0 60 40329.5 61 33283.0 62 28008.0 63 22317.0 64 16624.0 65 12672.5 66 9649.5 67 7346.0 68 5685.5 69 4446.5 70 3491.5 71 2564.0 72 1918.5 73 1489.5 74 1131.0 75 852.0 76 674.0 77 449.5 78 283.5 79 191.0 80 113.0 81 72.5 82 60.0 83 41.0 84 20.0 85 14.5 86 12.0 87 9.0 88 10.5 89 8.5 90 3.5 91 2.5 92 2.5 93 1.5 94 0.5 95 0.0 96 0.5 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007305333425577278 2 0.10430661500360672 3 0.11340199701690823 4 0.11678857542611622 5 0.15612126466448925 6 0.09380822193506187 7 0.024963920845019045 8 0.02535095837749996 9 0.021045165828649775 10-11 0.05026649953095889 12-13 0.05198397858134295 14-15 0.026052463905121618 16-17 0.030938812752693178 18-19 0.03560745298824422 20-21 0.057499263419196 22-23 0.03168869797187495 24-25 0.048694159555255176 26-27 0.05667680866267406 28-29 0.052491965342724156 30-31 0.06678816419873797 32-33 0.04939566508287683 34-35 0.061780866122266125 36-37 0.05162113089464209 38-39 0.03151936905141455 40-41 0.03466404900282199 42-43 0.030890433061133062 44-45 0.006652207589515734 46-47 0.012481960422509522 48-49 0.008103598336319167 50-51 0.008127788182099223 52-53 0.009409850008442256 54-55 0.010208114919184145 56-57 0.01405430039821324 58-59 0.018069814797702737 60-61 0.023585099635555783 62-63 0.010958000138365918 64-65 0.008829293709720883 66-67 0.009700128157802941 68-69 0.010837050909465632 70-71 0.02958418138900997 72-73 0.027479664806144995 74-75 0.027890892184405967 76-77 0.022883594107934124 78-79 0.004596070698210871 80-81 0.004305792548850184 82-83 0.005152437151152186 84-85 0.004958918384911729 86-87 0.012119112735808664 88-89 0.03580097175448468 90-91 0.03379321455473993 92-93 0.028423068791567224 94-95 0.04470283500154573 96-97 0.04105016828875709 98-99 0.02404470670537687 100-101 0.07273886626063204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2066983.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.14093627820907 #Duplication Level Percentage of deduplicated Percentage of total 1 80.0784115073411 49.76147466737888 2 11.760738950242358 14.616466593833236 3 3.621797229756968 6.751856126007656 4 1.5209328843090986 3.780487737891377 5 0.8172225997831697 2.5391488749119153 6 0.48054453149218845 1.7916892266178752 7 0.3364517462606983 1.4635198567554755 8 0.2374839595143926 1.1805980476224525 9 0.16806934434714813 0.9399587775656857 >10 0.9010581084632112 9.944096394313764 >50 0.05128508949268576 2.136827304212744 >100 0.022492112071878918 2.6057543546486417 >500 0.002263248240574174 1.0073729846667643 >1k 0.0011706456416762875 1.2336099851226807 >5k 7.804304277841916E-5 0.24713906845088576 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.24654290819034314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4009 0.19395418346449875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2714 0.13130248289415056 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.675938312022886E-5 0.0 0.0 0.0 0.0 8 9.675938312022886E-5 0.0 0.0 0.0 0.0 9 9.675938312022886E-5 0.0 0.0 0.0 0.0 10-11 9.675938312022886E-5 0.0 0.0 0.0 0.0 12-13 9.675938312022886E-5 0.0 0.0 0.0 0.0 14-15 9.675938312022886E-5 0.0 0.0 0.0 0.0 16-17 9.675938312022886E-5 0.0 0.0 2.4189845780057215E-5 0.0 18-19 9.675938312022886E-5 0.0 0.0 7.256953734017164E-5 0.0 20-21 9.675938312022886E-5 0.0 0.0 1.2094922890028607E-4 0.0 22-23 9.675938312022886E-5 0.0 0.0 2.9027814936068657E-4 0.0 24-25 9.675938312022886E-5 0.0 0.0 4.1122737826097263E-4 0.0 26-27 9.675938312022886E-5 0.0 0.0 7.982649107418881E-4 0.0 28-29 9.675938312022886E-5 0.0 0.0 0.002177086120205149 0.0 30-31 1.4513907468034328E-4 0.0 0.0 0.008708344480820597 0.0 32-33 1.4513907468034328E-4 0.0 0.0 0.022351417500772866 0.0 34-35 1.693289204604005E-4 0.0 0.0 0.04206614181151949 0.0 36-37 1.9351876624045772E-4 0.0 0.0 0.07643991266498079 0.0 38-39 1.9351876624045772E-4 0.0 0.0 0.12794009433072262 0.0 40-41 1.9351876624045772E-4 0.0 0.0 0.18481042175963713 0.0 42-43 1.9351876624045772E-4 0.0 0.0 0.24088248427780973 0.0 44-45 1.9351876624045772E-4 0.0 0.0 0.3157258671213068 0.0 46-47 1.9351876624045772E-4 0.0 0.0 0.40522829650751846 0.0 48-49 1.9351876624045772E-4 0.0 0.0 0.506511180788618 0.0 50-51 1.9351876624045772E-4 0.0 0.0 0.6123417560763683 0.0 52-53 1.9351876624045772E-4 0.0 0.0 0.7160920046270336 0.0 54-55 1.9351876624045772E-4 0.0 0.0 0.8345012997204138 0.0 56-57 1.9351876624045772E-4 0.0 0.0 0.9699644360887341 0.0 58-59 1.9351876624045772E-4 0.0 0.0 1.1207155549900507 0.0 60-61 1.9351876624045772E-4 0.0 0.0 1.2796670316108067 0.0 62-63 2.4189845780057213E-4 0.0 0.0 1.4411342521926884 0.0 64-65 2.4189845780057213E-4 4.837969156011443E-5 0.0 1.6097374772796873 0.0 66-67 3.38657840920801E-4 4.837969156011443E-5 0.0 1.8132708396730888 0.0 68-69 3.38657840920801E-4 4.837969156011443E-5 0.0 2.0376558491288996 0.0 70-71 3.38657840920801E-4 4.837969156011443E-5 0.0 2.2747889073108007 0.0 72-73 3.628476867008582E-4 4.837969156011443E-5 0.0 2.516034239275311 0.0 74-75 3.8703753248091544E-4 4.837969156011443E-5 0.0 2.7633270326848356 0.0 76-77 3.8703753248091544E-4 4.837969156011443E-5 0.0 3.039042894885928 0.0 78-79 3.8703753248091544E-4 4.837969156011443E-5 0.0 3.351357993752247 0.0 80-81 4.354172240410298E-4 4.837969156011443E-5 0.0 3.674897181060512 0.0 82-83 4.354172240410298E-4 4.837969156011443E-5 0.0 4.01263580784167 0.0 84-85 4.354172240410298E-4 4.837969156011443E-5 0.0 4.349382650945847 0.0 86-87 4.354172240410298E-4 4.837969156011443E-5 0.0 4.711770730576884 0.0 88-89 4.354172240410298E-4 4.837969156011443E-5 0.0 5.101106298406905 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2655 0.0 54.46598 1 GTATCAA 4920 0.0 43.894184 1 ATCAACG 6370 0.0 33.30356 3 TCAACGC 6450 0.0 33.037983 4 TATCAAC 6505 0.0 32.831764 2 CAACGCA 6585 0.0 32.288433 5 AACGCAG 6830 0.0 31.173405 6 ACGCAGA 7960 0.0 26.626043 7 CGCAGAG 8045 0.0 26.22595 8 TGGTATC 1185 0.0 25.28817 2 GTACATG 5425 0.0 24.81435 1 TACATGG 5330 0.0 24.452234 2 TAAGGTG 1145 0.0 23.263643 5 ACATGGG 5465 0.0 23.23894 3 GCAGAGT 9045 0.0 22.5384 9 GTGGTAT 1505 0.0 21.808653 1 AGAGTAC 8140 0.0 21.453432 10-11 CATGGGG 3935 0.0 20.791168 4 GAGTACT 6035 0.0 20.192974 12-13 GTAAGGT 1085 0.0 20.166164 4 >>END_MODULE