FastQCFastQC Report
Thu 26 May 2016
SRR938631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066983
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.25341282439187934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.2117095302670607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.14683236388494728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.054.718541
GTATCAA48600.043.3223341
ATCAACG65250.032.106443
TCAACGC66900.031.1725644
TATCAAC67050.031.1178862
CAACGCA68350.030.5112575
AACGCAG71700.029.2054846
ACGCAGA83800.024.8751377
CGCAGAG84350.024.7692348
TTACGCT1551.4104007E-624.5184154
TACATGG53900.023.8078042
TGGTATC13900.023.5927542
GTAAGGT9650.023.1368734
GTGGTAT15150.022.9010071
GTACATG58000.022.8623351
ACATGGG55800.022.3049473
GCAGAGT95600.021.357759
TAAGGTG11600.020.8855695
GGTAAGG11250.020.2685573
AGAGTAC86600.020.1230210-11
Produced by FastQC (version 0.11.5)