##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2066983 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.597285996062862 31.0 31.0 34.0 30.0 34.0 2 31.841354283029904 31.0 31.0 34.0 30.0 34.0 3 31.898038348646313 31.0 31.0 34.0 30.0 34.0 4 35.60743557155526 37.0 35.0 37.0 33.0 37.0 5 35.49535530771177 37.0 35.0 37.0 33.0 37.0 6 35.49812020708443 37.0 35.0 37.0 33.0 37.0 7 35.43125318398845 37.0 35.0 37.0 33.0 37.0 8 35.40455727018558 37.0 35.0 37.0 33.0 37.0 9 36.960934850455956 39.0 37.0 39.0 33.0 39.0 10-11 36.83983757969949 39.0 37.0 39.0 32.5 39.0 12-13 36.715683438131805 39.0 35.0 39.0 32.0 39.0 14-15 37.68880005302414 39.0 36.5 41.0 32.0 41.0 16-17 37.57246818188635 39.0 36.0 41.0 32.0 41.0 18-19 37.824285685949036 39.5 37.0 41.0 32.0 41.0 20-21 37.7902522662257 40.0 37.0 41.0 32.0 41.0 22-23 37.66114186715615 39.0 37.0 41.0 32.0 41.0 24-25 37.51836855939308 39.0 36.0 41.0 32.0 41.0 26-27 37.29100723131249 39.0 36.0 41.0 31.0 41.0 28-29 37.15908040849877 39.0 36.0 40.5 31.0 41.0 30-31 36.877232662290886 39.0 36.0 40.0 30.5 41.0 32-33 36.65841325255215 38.5 35.0 40.0 30.0 41.0 34-35 36.54824398652529 39.0 35.0 40.0 30.0 41.0 36-37 36.49325877377801 39.0 35.0 40.0 30.0 41.0 38-39 36.298921181257896 38.0 35.0 40.0 30.0 41.0 40-41 36.105098348655986 38.0 35.0 40.0 29.5 41.0 42-43 35.86405403430991 38.0 35.0 40.0 29.0 41.0 44-45 35.546468209946575 38.0 34.0 40.0 27.5 41.0 46-47 35.13638597898483 38.0 34.0 40.0 26.5 41.0 48-49 34.740053014466014 38.0 33.0 40.0 25.5 41.0 50-51 34.30987240823945 38.0 33.0 40.0 24.0 41.0 52-53 33.74169913347134 37.0 32.5 40.0 23.0 41.0 54-55 33.3383678046699 37.0 32.0 39.0 22.5 40.5 56-57 32.945989396139204 36.0 31.0 39.0 21.0 40.0 58-59 32.39244493060659 36.0 31.0 39.0 19.0 40.0 60-61 31.9254868085514 35.0 30.0 39.0 17.5 40.0 62-63 31.371657870432415 35.0 29.5 38.0 13.5 40.0 64-65 30.765133046570774 34.0 28.5 38.0 9.5 40.0 66-67 30.175002648788112 34.0 28.0 37.5 8.0 39.5 68-69 29.583992950111345 34.0 28.0 37.0 7.0 39.0 70-71 28.908850725913084 33.0 26.0 36.0 3.5 39.0 72-73 28.31259424968662 33.0 26.0 36.0 2.0 38.5 74-75 27.64702539885427 32.0 25.5 35.0 2.0 38.0 76-77 26.549014191214923 30.5 24.0 34.0 2.0 36.0 78-79 27.21005421912033 32.0 25.5 35.0 2.0 37.0 80-81 26.748031551299647 32.0 24.5 35.0 2.0 36.0 82-83 26.20862580872702 31.5 23.5 34.5 2.0 36.0 84-85 25.52860449263492 31.0 21.0 34.0 2.0 35.0 86-87 24.769980691665097 31.0 19.0 34.0 2.0 35.0 88-89 23.86027727368827 30.0 14.0 34.0 2.0 35.0 90-91 23.08009886873767 29.5 4.5 34.0 2.0 35.0 92-93 22.071585252515384 29.0 2.0 34.0 2.0 35.0 94-95 21.061681929653027 29.0 2.0 33.5 2.0 35.0 96-97 19.784766976796618 26.5 2.0 33.0 2.0 34.0 98-99 18.233887990370505 24.5 2.0 32.5 2.0 34.0 100-101 14.640824089990097 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3428015326938265 1101 2 0.38528105523790046 1101 3 0.3444760603765964 1101 4 0.204234276446833 1101 5 0.12335975272907262 1101 6 0.21805601495437088 1101 7 0.3049697214798641 1101 8 0.25760055031797435 1101 9 0.41735740386269526 1101 10-11 0.3326539074894441 1101 12-13 0.3720760019237801 1101 14-15 0.4771078321785893 1101 16-17 0.45400770360051723 1101 18-19 0.5413399420608727 1101 20-21 0.4973633162482116 1101 22-23 0.43710241345029033 1101 24-25 0.49608413536007845 1101 26-27 0.5307296117209361 1101 28-29 0.6137477679150223 1101 30-31 0.6651655708875097 1101 32-33 0.5431175362058198 1101 34-35 0.5684083584300055 1101 36-37 0.7465518221722149 1101 38-39 0.8038768299512924 1101 40-41 0.9839179617623088 1101 42-43 1.0364704258544961 1101 44-45 0.776914173461833 1101 46-47 0.8109320692923419 1101 48-49 0.7253651895255544 1101 50-51 0.8610250346941513 1101 52-53 0.9346634044644233 1101 54-55 0.9534273513668623 1101 56-57 0.8561064900433664 1101 58-59 1.0829376184156772 1101 60-61 1.262131835444972 1101 62-63 1.2470682412419336 1101 64-65 1.1725108187179778 1101 66-67 0.9105270578428417 1101 68-69 0.9753436052381588 1101 70-71 1.1404182257121782 1101 72-73 1.284295561963674 1101 74-75 1.3157439361281327 1101 76-77 1.2008643556751508 1101 78-79 1.4797144843291683 1101 80-81 1.5770700631815515 1101 82-83 1.5196450966136439 1101 84-85 1.604476933763479 1101 86-87 1.6622007874876807 1101 88-89 1.608650862300145 1101 90-91 1.7152243757433254 1101 92-93 1.9606278208877974 1101 94-95 1.8987459529690653 1101 96-97 1.9365853436403277 1101 98-99 2.090632138958359 1101 100-101 1.8162784021396021 1102 1 0.2794769780657731 1102 2 0.2976025729922931 1102 3 0.26911568366777416 1102 4 0.3170933735740533 1102 5 0.18767074844519271 1102 6 0.21488110157816465 1102 7 0.1698164353592162 1102 8 0.25435273956576765 1102 9 0.32143066107256146 1102 10-11 0.4225053519431867 1102 12-13 0.39583124045330464 1102 14-15 0.5545132129355395 1102 16-17 0.4825873525143507 1102 18-19 0.5419425086689174 1102 20-21 0.561893779596339 1102 22-23 0.5344460020485187 1102 24-25 0.4713990675344988 1102 26-27 0.5330142465050827 1102 28-29 0.44940606224050583 1102 30-31 0.641750003110019 1102 32-33 0.5962558457932161 1102 34-35 0.5038243853058688 1102 36-37 0.7683271989896099 1102 38-39 0.6020914646423847 1102 40-41 0.5748101325841262 1102 42-43 0.5334917149903049 1102 44-45 0.7842023600542944 1102 46-47 0.7749572142340355 1102 48-49 0.8834664145116804 1102 50-51 0.9470834890836528 1102 52-53 0.7805692086465612 1102 54-55 0.7639670711307289 1102 56-57 0.7726537290940243 1102 58-59 0.9352087594302176 1102 60-61 0.9674604785301106 1102 62-63 0.9782304735767298 1102 64-65 0.9633922875845933 1102 66-67 0.8545829747832627 1102 68-69 1.1750893950269408 1102 70-71 1.2314406812060028 1102 72-73 1.0628938229735034 1102 74-75 1.226374079658413 1102 76-77 1.235245550013353 1102 78-79 1.4960551291142146 1102 80-81 1.7253355709350409 1102 82-83 1.7165122155542463 1102 84-85 1.7731824004462773 1102 86-87 1.530448086407251 1102 88-89 1.5427609404399263 1102 90-91 1.7421607316810501 1102 92-93 2.0940152844003315 1102 94-95 2.3433493757335206 1102 96-97 1.9414237130966363 1102 98-99 2.0695686748130093 1102 100-101 1.9409429966595795 1103 1 0.24984874181619432 1103 2 0.2695700412648101 1103 3 0.3487102441964147 1103 4 0.2580491071694553 1103 5 0.24360827497095272 1103 6 0.18465975462610373 1103 7 0.2507262471292151 1103 8 0.1713581895301317 1103 9 0.3098985927861335 1103 10-11 0.2526417311484437 1103 12-13 0.3946298767039096 1103 14-15 0.5310827809180552 1103 16-17 0.47768901736180425 1103 18-19 0.5257599278247866 1103 20-21 0.5209342710784526 1103 22-23 0.3637788772726154 1103 24-25 0.5048972391497273 1103 26-27 0.612799472610142 1103 28-29 0.4592788708126889 1103 30-31 0.4595752297439901 1103 32-33 0.5007945609290445 1103 34-35 0.5570687072466214 1103 36-37 0.641751382124113 1103 38-39 0.6776171066031367 1103 40-41 0.6826801421685857 1103 42-43 0.8887553218796924 1103 44-45 0.7890152795878862 1103 46-47 0.736341356744397 1103 48-49 0.8513918365458792 1103 50-51 1.0442417828869424 1103 52-53 0.890857088074263 1103 54-55 0.8727112845157947 1103 56-57 0.8610405532922627 1103 58-59 0.977329216566936 1103 60-61 1.0930297560355449 1103 62-63 1.10208695998125 1103 64-65 1.2489590953949765 1103 66-67 1.1372810547766434 1103 68-69 1.2961051989425378 1103 70-71 1.0561729496273067 1103 72-73 1.0009452122516151 1103 74-75 1.2742536395834954 1103 76-77 1.2873989833373436 1103 78-79 1.567479755002335 1103 80-81 1.625082160601817 1103 82-83 1.636156592263358 1103 84-85 1.6398042585697574 1103 86-87 1.6512555231325088 1103 88-89 1.5759331251048678 1103 90-91 1.6844824753625325 1103 92-93 1.8096483474321552 1103 94-95 1.6996390364127727 1103 96-97 1.756115860703357 1103 98-99 2.178099722377457 1103 100-101 2.3770835713606076 1104 1 0.22747318242721093 1104 2 0.22327342930406147 1104 3 0.27300380608091857 1104 4 0.16852186898850618 1104 5 0.1441057874087619 1104 6 0.1141693560025061 1104 7 0.08526529242352865 1104 8 0.19599289630689043 1104 9 0.3322968800160382 1104 10-11 0.289260828444057 1104 12-13 0.3662984866009751 1104 14-15 0.4893510558067433 1104 16-17 0.5630809248867621 1104 18-19 0.38585415745325946 1104 20-21 0.4107292432254823 1104 22-23 0.46836217367320643 1104 24-25 0.5353741374358165 1104 26-27 0.4393610457403767 1104 28-29 0.6563519794585986 1104 30-31 0.5414886412156363 1104 32-33 0.6004027329515225 1104 34-35 0.7242558414704305 1104 36-37 0.8400177024184785 1104 38-39 0.6016629350215794 1104 40-41 0.638661996260403 1104 42-43 0.7785514304187657 1104 44-45 0.7369526143848901 1104 46-47 0.591690013188078 1104 48-49 0.5894136961832785 1104 50-51 0.8152031023744684 1104 52-53 0.7105468985013346 1104 54-55 0.7371556228969851 1104 56-57 0.7794623774742249 1104 58-59 1.0524890501082282 1104 60-61 1.0740417813361738 1104 62-63 1.0202871877142172 1104 64-65 0.782029240330413 1104 66-67 1.0035095286703104 1104 68-69 1.227317115604091 1104 70-71 1.1740861247294099 1104 72-73 1.1962523676577739 1104 74-75 1.4033352757153068 1104 76-77 1.2986356278898192 1104 78-79 1.5019497076602413 1104 80-81 1.2616253469087475 1104 82-83 1.664042516684198 1104 84-85 1.8928878583870272 1104 86-87 2.2665584057045756 1104 88-89 2.009598512194092 1104 90-91 2.168349117483128 1104 92-93 2.5136091282698594 1104 94-95 2.6255394566505537 1104 96-97 2.6155907566666663 1104 98-99 2.999235023487259 1104 100-101 2.5795113593280306 1105 1 0.33719996276644437 1105 2 0.2947721827379617 1105 3 0.2798459946009224 1105 4 0.24600725887739117 1105 5 0.12627733005380293 1105 6 0.15165100285111066 1105 7 0.3941797439010273 1105 8 0.3652384731695548 1105 9 0.3179797509279396 1105 10-11 0.36703028835454177 1105 12-13 0.3996538771359823 1105 14-15 0.5171832339219833 1105 16-17 0.3406997719857898 1105 18-19 0.45000385232862783 1105 20-21 0.5073531545688965 1105 22-23 0.41235359141655437 1105 24-25 0.5186763901667888 1105 26-27 0.493701969870294 1105 28-29 0.551736231507796 1105 30-31 0.7306102075888816 1105 32-33 0.6774480928069693 1105 34-35 0.6984934755124854 1105 36-37 0.6277494422860883 1105 38-39 0.5744512549534875 1105 40-41 0.5715524031600694 1105 42-43 0.8176305533887742 1105 44-45 0.6570309063108368 1105 46-47 0.6117787767148357 1105 48-49 0.7784104945470389 1105 50-51 0.7639869484820423 1105 52-53 0.8177125926459254 1105 54-55 0.8416198668362682 1105 56-57 0.765322374399581 1105 58-59 0.8771755147843976 1105 60-61 0.8330813350231843 1105 62-63 1.0220517638483244 1105 64-65 0.9138655803499773 1105 66-67 0.877971745129436 1105 68-69 1.0873363209655516 1105 70-71 1.1680905762737481 1105 72-73 1.0084007613810506 1105 74-75 1.0889867318248179 1105 76-77 1.206650412898945 1105 78-79 1.4309934722675663 1105 80-81 1.3983889093027813 1105 82-83 1.5720327936914558 1105 84-85 1.5283558386825966 1105 86-87 1.4245960277154346 1105 88-89 1.5330170219738974 1105 90-91 1.856519327784067 1105 92-93 2.1380919537305516 1105 94-95 2.0535717091810035 1105 96-97 2.1345744321094244 1105 98-99 2.47839542390345 1105 100-101 2.2304806508016988 1106 1 5.559732093018965E-5 1106 2 0.07937216931731328 1106 3 0.07216103037141508 1106 4 0.14024807683154705 1106 5 0.0659054888480668 1106 6 0.013766190546604662 1106 7 0.08346250341372041 1106 8 0.12364731117440186 1106 9 -0.040476890540212196 1106 10-11 0.17937321150590435 1106 12-13 0.0868226815446036 1106 14-15 0.13595588507003242 1106 16-17 0.18790404988893528 1106 18-19 0.13546334842174446 1106 20-21 0.23933284032701607 1106 22-23 0.30391372576091413 1106 24-25 0.3346369083174068 1106 26-27 0.10274870434435002 1106 28-29 0.2623278206526365 1106 30-31 0.175843616411683 1106 32-33 0.24299945345206453 1106 34-35 0.24847607124054605 1106 36-37 0.29752603711849446 1106 38-39 0.19638961848970382 1106 40-41 0.16217554502343745 1106 42-43 0.24765453418468297 1106 44-45 0.18196949467335344 1106 46-47 -0.5369102670273662 1106 48-49 -0.23397532602941595 1106 50-51 0.22161347611996973 1106 52-53 0.37506803126297683 1106 54-55 0.6007397762894513 1106 56-57 0.4306445095317457 1106 58-59 0.07485520198188311 1106 60-61 0.3248366567062284 1106 62-63 0.36855500538130315 1106 64-65 0.5306036447394966 1106 66-67 0.35201464701455265 1106 68-69 0.3421192520882208 1106 70-71 0.5496525290959049 1106 72-73 0.7783945396325862 1106 74-75 0.7424414240550519 1106 76-77 0.5178176100732301 1106 78-79 0.8909758105299836 1106 80-81 0.39243760112390547 1106 82-83 0.9112937497161226 1106 84-85 1.2268522190603086 1106 86-87 1.3167910074597806 1106 88-89 1.3462258000520961 1106 90-91 1.2502584931939857 1106 92-93 1.154439197439217 1106 94-95 1.2741547897959293 1106 96-97 1.3436293365562229 1106 98-99 1.4127102622160415 1106 100-101 1.2439990161097647 1107 1 0.15732236909574837 1107 2 0.2143813092436453 1107 3 0.17458344106250578 1107 4 0.15864060254521917 1107 5 0.13127807643385836 1107 6 0.12219769753573217 1107 7 0.20509296860735304 1107 8 0.13705860705865547 1107 9 0.2846223037773896 1107 10-11 0.26291888251898854 1107 12-13 0.19984506525820933 1107 14-15 0.35078928304528034 1107 16-17 0.23550611506799868 1107 18-19 0.2318484445180573 1107 20-21 0.330346253424068 1107 22-23 0.3461468096925344 1107 24-25 0.32678781896787257 1107 26-27 0.25952235638033017 1107 28-29 0.37570823651427787 1107 30-31 0.23461482366246145 1107 32-33 0.30524271198090247 1107 34-35 0.4420659949470789 1107 36-37 0.49818934720875063 1107 38-39 0.5286493557033154 1107 40-41 0.4305468569821116 1107 42-43 0.4428559027520649 1107 44-45 0.4372937469185061 1107 46-47 0.4344130390042622 1107 48-49 0.6160900563645271 1107 50-51 0.6288002960540098 1107 52-53 0.668927469722874 1107 54-55 0.3907747349566506 1107 56-57 0.4398231609693326 1107 58-59 0.43793372209432846 1107 60-61 0.3918357989500656 1107 62-63 0.6133455275558788 1107 64-65 0.5843847163863565 1107 66-67 0.6096682095845196 1107 68-69 0.7663326120308085 1107 70-71 0.8912754373896412 1107 72-73 1.1200907954848773 1107 74-75 0.9326545582512651 1107 76-77 0.9723042992424844 1107 78-79 1.485749061505917 1107 80-81 1.524406569803201 1107 82-83 1.3946842801360475 1107 84-85 1.4918951729115832 1107 86-87 1.7088319067789008 1107 88-89 1.6660764912402648 1107 90-91 1.8161545201778786 1107 92-93 2.2214396405265155 1107 94-95 2.163812043559272 1107 96-97 2.2171384198458277 1107 98-99 2.2061963586009377 1107 100-101 2.0894550097760565 1108 1 0.05926284173607854 1108 2 0.0971263272671763 1108 3 0.07244311386606839 1108 4 0.07102635322927853 1108 5 0.045238597311332285 1108 6 0.07250611183108902 1108 7 0.017501739187927967 1108 8 -0.11291915781403361 1108 9 0.10826373749839746 1108 10-11 0.04565945351706091 1108 12-13 0.06564560831849064 1108 14-15 0.2669798563976542 1108 16-17 0.1958255684500969 1108 18-19 0.1472137022624196 1108 20-21 0.20030476529239394 1108 22-23 0.2085231431461949 1108 24-25 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1163.0 9 2046.0 10 3290.0 11 5202.0 12 7203.0 13 9955.0 14 13191.0 15 17345.0 16 21155.0 17 25351.0 18 25523.0 19 24134.0 20 25152.0 21 26595.0 22 29496.0 23 32380.0 24 37400.0 25 42518.0 26 50492.0 27 58911.0 28 69716.0 29 80509.0 30 94600.0 31 109064.0 32 129089.0 33 153532.0 34 185866.0 35 225307.0 36 259244.0 37 225986.0 38 73182.0 39 1552.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.638392686517555 19.599395639138702 15.32794027219461 23.43427140214913 2 16.35258908894834 20.58207248122819 38.84153752401383 24.22380090580964 3 19.768183869920556 23.5588778427302 30.00063377395944 26.672304513389804 4 13.97703803079174 17.018959517325495 35.72617675133274 33.27782570055003 5 14.065636960987216 36.97028012305357 34.27674082277072 14.687342093188494 6 33.36007117620222 35.99187801738089 16.488282680602598 14.15976812581429 7 29.04992445511163 32.053674365004454 20.217002268523736 18.67939891136018 8 26.42184285018309 35.87673435146782 18.948099718285054 18.753323080064035 9 25.80350201235327 16.84426045110192 19.63881657468881 37.713420961855995 10-11 25.266535815727558 26.497871535469812 27.05443150717737 21.181161141625257 12-13 26.019928562547438 23.42682547461687 27.536051336658307 23.017194626177382 14-15 22.498951370185434 25.152045275650547 25.524278622514068 26.824724731649948 16-17 22.503595820575207 28.232791464661293 26.433236267545503 22.830376447217997 18-19 22.47529370101254 26.85186573861517 28.48342245678847 22.189418103583822 20-21 22.85183284042491 26.09600562752572 28.83867453191439 22.21348700013498 22-23 22.624303151017692 25.962308349899345 28.737052989792367 22.676335509290592 24-25 22.601165634020333 26.202308044384274 28.728461268263228 22.468065053332158 26-27 22.32483745935123 26.518975231313664 28.499918599652773 22.656268709682337 28-29 22.370283035983398 26.80530203228767 28.433449557591484 22.39096537413745 30-31 22.545726179702903 26.43186181768746 28.373417747546448 22.648994255063197 32-33 22.323744759247095 26.348468824474814 28.405219417370514 22.92256699890758 34-35 22.884179687263746 26.47935231878921 28.21603405334201 22.42043394060503 36-37 22.8753631379669 26.205187377618238 28.24451842225793 22.67493106215693 38-39 22.517336966474165 26.437195633941386 28.283754620751818 22.76171277883263 40-41 22.911191137328878 26.297466111487804 28.260540895415808 22.530801855767514 42-43 22.626530721347333 26.32887888169985 28.134793223956844 22.909797172995976 44-45 22.687096783558108 26.550523558436208 28.23153756160436 22.530842096401326 46-47 23.01654150034132 26.77184572877474 27.545316047592074 22.66629672329187 48-49 22.816007678824647 26.83050610479138 27.55213758410205 22.80134863228193 50-51 22.93728359033581 26.905118649463393 27.552345920673293 22.6052518395275 52-53 23.166228266028313 26.704549577814625 27.487623265406636 22.64159889075043 54-55 22.796715068819886 26.892598212780307 27.55840903048859 22.752277687911217 56-57 22.799413444619525 27.028741890958948 27.476157278506886 22.69568738591464 58-59 22.782172260104858 26.768321112040468 27.419577666765786 23.029928961088892 60-61 22.546823074984168 26.929684472489612 27.60025118735858 22.923241265167636 62-63 22.62326190355807 26.916354369550927 27.68052773152423 22.77985599536677 64-65 22.496787721560114 26.859147729485873 27.521151683171002 23.12291286578301 66-67 22.398882944289458 26.940139206197607 27.446800995905875 23.214176853607064 68-69 22.774125759587122 26.67811989893865 27.553080070287134 22.994674271187087 70-71 22.65046333253023 26.736976906426857 27.285481924854693 23.32707783618822 72-73 22.56936019988568 26.75539186006261 27.326441963800836 23.348805976250876 74-75 22.586174149630235 27.05006345185341 27.02480368637727 23.338958712139082 76-77 22.610354621738104 26.832862530832884 27.333497581772487 23.223285265656525 78-79 22.628723828217723 26.950018426018747 27.350865998684164 23.07039174707936 80-81 22.738334721380067 26.916968351082748 27.15285564201073 23.191841285526454 82-83 22.70269707821568 26.973613366601256 27.229293390528607 23.09439616465445 84-85 22.620048774350884 27.02720815228436 27.153208558737283 23.19953451462747 86-87 22.70731973430289 26.976622437843815 27.010443008347 23.305614819506292 88-89 22.619513940569494 27.104666966541956 26.726586849484068 23.54923224340448 90-91 22.56152443556629 27.061146017219485 27.01014180525876 23.367187741955465 92-93 22.605162262514778 26.764229514971472 27.012935416794704 23.61767280571905 94-95 22.371137687431087 26.645453249870126 27.175708016537463 23.80770104616132 96-97 22.54964166240766 26.449733037525746 27.118760550728677 23.881864749337918 98-99 22.32884208457643 26.615701146203858 26.856128290413917 24.199328478805793 100-101 22.504685567966803 26.377225538940113 27.166157364924153 23.95193152816893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 256.0 1 181.0 2 159.5 3 220.5 4 210.0 5 223.5 6 384.5 7 786.0 8 1218.5 9 1393.5 10 1242.5 11 1066.5 12 1078.0 13 1196.5 14 1408.0 15 1560.5 16 1717.0 17 1905.5 18 2140.0 19 2437.0 20 2846.5 21 3437.5 22 4157.0 23 5169.0 24 6590.0 25 8487.5 26 10910.5 27 13812.5 28 16541.5 29 19354.5 30 22525.5 31 27089.5 32 33098.5 33 35753.5 34 36626.5 35 39497.5 36 43870.5 37 48566.5 38 51961.5 39 54563.0 40 57991.5 41 62238.0 42 64582.5 43 66753.5 44 70091.0 45 73810.0 46 75883.0 47 77602.5 48 78839.0 49 78297.5 50 77599.5 51 75605.5 52 74204.0 53 74554.5 54 75057.0 55 71035.5 56 64790.0 57 59089.5 58 53581.5 59 47244.5 60 38875.0 61 32242.5 62 27005.5 63 20979.5 64 15440.0 65 11608.5 66 8785.5 67 6844.5 68 5274.0 69 3961.0 70 3071.0 71 2227.5 72 1674.5 73 1339.5 74 1014.5 75 751.0 76 553.5 77 379.5 78 226.0 79 145.5 80 92.0 81 51.0 82 34.0 83 20.5 84 8.0 85 6.0 86 3.5 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.9027814936068657E-4 2 0.04770237587827283 3 0.0 4 0.0 5 0.043106305180061956 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006047461445014304 26-27 0.0018626181250644056 28-29 9.675938312022886E-5 30-31 0.001572339975703719 32-33 4.354172240410298E-4 34-35 0.010909620446805803 36-37 0.021649911973151206 38-39 0.0040880839368296696 40-41 0.0010401633685424602 42-43 0.0011853024432228036 44-45 0.00798264910741888 46-47 0.0 48-49 0.0 50-51 5.805562987213731E-4 52-53 0.0 54-55 0.0014272009010233757 56-57 0.0 58-59 0.021262874440670293 60-61 0.0 62-63 0.008442256177239968 64-65 0.01468323638849473 66-67 0.005853942678773846 68-69 0.015360552070336332 70-71 4.837969156011443E-5 72-73 7.256953734017164E-5 74-75 0.022036949505632123 76-77 0.04871834940103523 78-79 0.02958418138900997 80-81 0.025641236526860647 82-83 0.03524460530154336 84-85 0.01562664037391696 86-87 0.009361470316882142 88-89 0.08127788182099223 90-91 0.023560909789775725 92-93 0.023512530098215613 94-95 0.025326768531719904 96-97 0.03749426095908868 98-99 0.05208073796446318 100-101 0.012796428417650267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2066983.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.54091149218637 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69246223597611 47.641133913304465 2 12.596696476609361 15.252309729686864 3 3.8398164362898233 6.973979610469939 4 1.6323136011991035 3.952870130307476 5 0.8724782865613665 2.6410315362783052 6 0.5281124459705387 1.9183445309654663 7 0.35092183110530806 1.4871589262335698 8 0.2509142275048221 1.2152460831599798 9 0.1848935073996231 1.0074259320264436 >10 0.9677375525425883 10.34814962595383 >50 0.0563678854658144 2.2994538732549645 >100 0.023526836636221227 2.6823401397860542 >500 0.0025590990565574614 1.1233407051185984 >1k 0.0011196058372438737 1.203613909544353 >5k 7.997184551741956E-5 0.25360135390962674 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5238 0.25341282439187934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4376 0.2117095302670607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3035 0.14683236388494728 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.837969156011443E-5 0.0 10-11 0.0 0.0 0.0 4.837969156011443E-5 0.0 12-13 0.0 0.0 0.0 4.837969156011443E-5 0.0 14-15 4.837969156011443E-5 0.0 0.0 4.837969156011443E-5 0.0 16-17 4.837969156011443E-5 0.0 0.0 7.256953734017164E-5 0.0 18-19 4.837969156011443E-5 0.0 0.0 1.2094922890028607E-4 0.0 20-21 4.837969156011443E-5 0.0 0.0 1.693289204604005E-4 0.0 22-23 4.837969156011443E-5 0.0 0.0 2.9027814936068657E-4 0.0 24-25 4.837969156011443E-5 0.0 0.0 4.1122737826097263E-4 0.0 26-27 4.837969156011443E-5 0.0 0.0 7.982649107418881E-4 0.0 28-29 9.675938312022886E-5 0.0 0.0 0.002177086120205149 0.0 30-31 9.675938312022886E-5 0.0 0.0 0.008659964789260483 0.0 32-33 9.675938312022886E-5 0.0 0.0 0.022254658117652638 0.0 34-35 9.675938312022886E-5 0.0 0.0 0.04194519258261921 0.0 36-37 9.675938312022886E-5 0.0 0.0 0.07610125482406 0.0 38-39 9.675938312022886E-5 0.0 0.0 0.12786752479338243 0.0 40-41 9.675938312022886E-5 0.0 0.0 0.184762042068077 0.0 42-43 9.675938312022886E-5 0.0 0.0 0.24064058582000916 0.0 44-45 9.675938312022886E-5 0.0 0.0 0.3155565382008464 0.0 46-47 1.4513907468034328E-4 0.0 0.0 0.4051315371243982 0.0 48-49 1.4513907468034328E-4 0.0 0.0 0.5060515737187969 0.0 50-51 1.4513907468034328E-4 0.0 0.0 0.6108661754837847 0.0 52-53 1.4513907468034328E-4 0.0 0.0 0.7134069317454474 0.0 54-55 1.4513907468034328E-4 0.0 0.0 0.8307518736245049 0.0 56-57 1.9351876624045772E-4 0.0 0.0 0.9646426700171216 0.0 58-59 1.9351876624045772E-4 0.0 0.0 1.114474574778796 0.0 60-61 1.9351876624045772E-4 0.0 0.0 1.2720714200358687 0.0 62-63 1.9351876624045772E-4 0.0 0.0 1.4315066935722256 0.0 64-65 1.9351876624045772E-4 4.837969156011443E-5 0.0 1.5974732254691983 0.0 66-67 1.9351876624045772E-4 4.837969156011443E-5 0.0 1.7989746408170748 0.0 68-69 1.9351876624045772E-4 4.837969156011443E-5 0.0 2.022174347829663 0.0 70-71 1.9351876624045772E-4 4.837969156011443E-5 0.0 2.2566707128215375 4.837969156011443E-5 72-73 1.9351876624045772E-4 4.837969156011443E-5 0.0 2.495835718048963 4.837969156011443E-5 74-75 1.9351876624045772E-4 4.837969156011443E-5 0.0 2.742112537935726 4.837969156011443E-5 76-77 1.9351876624045772E-4 4.837969156011443E-5 0.0 3.018070298594618 4.837969156011443E-5 78-79 1.9351876624045772E-4 4.837969156011443E-5 0.0 3.3306031060729575 4.837969156011443E-5 80-81 1.9351876624045772E-4 4.837969156011443E-5 0.0 3.6559323419689473 4.837969156011443E-5 82-83 2.4189845780057213E-4 4.837969156011443E-5 0.0 3.995944814253431 4.837969156011443E-5 84-85 2.660883035806293E-4 4.837969156011443E-5 0.0 4.333368973039449 4.837969156011443E-5 86-87 2.9027814936068657E-4 4.837969156011443E-5 0.0 4.697982518482252 4.837969156011443E-5 88-89 2.9027814936068657E-4 4.837969156011443E-5 0.0 5.089422602895137 4.837969156011443E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2875 0.0 54.71854 1 GTATCAA 4860 0.0 43.322334 1 ATCAACG 6525 0.0 32.10644 3 TCAACGC 6690 0.0 31.172564 4 TATCAAC 6705 0.0 31.117886 2 CAACGCA 6835 0.0 30.511257 5 AACGCAG 7170 0.0 29.205484 6 ACGCAGA 8380 0.0 24.875137 7 CGCAGAG 8435 0.0 24.769234 8 TTACGCT 155 1.4104007E-6 24.518415 4 TACATGG 5390 0.0 23.807804 2 TGGTATC 1390 0.0 23.592754 2 GTAAGGT 965 0.0 23.136873 4 GTGGTAT 1515 0.0 22.901007 1 GTACATG 5800 0.0 22.862335 1 ACATGGG 5580 0.0 22.304947 3 GCAGAGT 9560 0.0 21.35775 9 TAAGGTG 1160 0.0 20.885569 5 GGTAAGG 1125 0.0 20.268557 3 AGAGTAC 8660 0.0 20.12302 10-11 >>END_MODULE