FastQCFastQC Report
Thu 26 May 2016
SRR938630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120747
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.25255252040908227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.1907818330050685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.12990705633439537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.055.0066381
GTATCAA49050.043.0891761
ATCAACG61750.034.0174563
TCAACGC62550.033.5064054
TATCAAC64450.032.7398452
CAACGCA64500.032.3445325
AACGCAG67350.031.180896
TGGTATC11900.026.757432
ACGCAGA79350.026.2820937
CGCAGAG79450.026.0696358
GTGGTAT13850.025.4335061
GTACATG57500.022.7661271
TACATGG55900.022.6994822
GCAGAGT89100.022.4458739
ACATGGG56750.021.5220583
TAAGGTG11200.021.2152425
GAGTACT56450.020.78425212-13
GTGTAGC14350.020.5666731
CAGAGTA82000.020.36332510-11
GTACTTT57600.020.20506914-15
Produced by FastQC (version 0.11.5)