##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938630_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2120747 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18756504194041 31.0 31.0 34.0 28.0 34.0 2 31.290737650459956 31.0 31.0 34.0 28.0 34.0 3 31.36194652167373 31.0 31.0 34.0 28.0 34.0 4 35.008720040627196 37.0 35.0 37.0 32.0 37.0 5 35.018100697537236 37.0 35.0 37.0 32.0 37.0 6 35.050519934721116 37.0 35.0 37.0 32.0 37.0 7 34.97318916400683 37.0 35.0 37.0 32.0 37.0 8 34.93504600030084 37.0 35.0 37.0 32.0 37.0 9 36.46633143887507 39.0 37.0 39.0 32.0 39.0 10-11 36.31269760136405 39.0 35.0 39.0 32.0 39.0 12-13 36.16424542861549 39.0 35.0 39.0 32.0 39.0 14-15 37.17364824752788 39.0 36.0 41.0 31.5 41.0 16-17 37.01103856329868 39.0 36.0 41.0 31.0 41.0 18-19 36.989962970594796 39.0 36.0 41.0 31.0 41.0 20-21 37.080286097304395 39.0 36.0 41.0 31.0 41.0 22-23 37.000590122254096 39.0 36.0 41.0 31.0 41.0 24-25 36.88316946811666 39.0 36.0 41.0 30.5 41.0 26-27 36.686908433679264 39.0 36.0 41.0 30.0 41.0 28-29 36.50097630693335 39.0 36.0 40.5 30.0 41.0 30-31 36.28065747587996 39.0 35.0 40.0 29.5 41.0 32-33 36.00221832212895 38.0 35.0 40.0 29.0 41.0 34-35 35.74723104641902 38.0 35.0 40.0 27.5 41.0 36-37 35.45714835385833 38.0 34.0 40.0 27.0 41.0 38-39 35.193148216171 38.0 34.0 40.0 26.0 41.0 40-41 35.12091447023148 38.0 34.0 40.0 25.5 41.0 42-43 34.92616422420968 38.0 34.0 40.0 25.0 41.0 44-45 34.6392572994327 38.0 33.0 40.0 24.0 41.0 46-47 34.28699439395647 38.0 33.0 40.0 23.5 41.0 48-49 33.91969103339531 38.0 33.0 40.0 22.5 41.0 50-51 33.195510119783265 37.5 32.0 39.5 21.0 40.5 52-53 33.26403361645684 37.0 32.0 39.5 21.0 40.0 54-55 33.378087768130754 37.0 32.5 40.0 20.5 41.0 56-57 33.059590559364224 37.0 32.0 40.0 18.5 41.0 58-59 32.716151903079435 36.5 31.0 39.5 17.5 41.0 60-61 32.246847690931546 36.0 31.0 39.0 13.5 41.0 62-63 31.70494382403936 35.5 30.5 39.0 8.5 40.0 64-65 31.19248052690868 35.0 29.5 38.5 7.5 40.0 66-67 30.626108630591013 35.0 29.0 38.0 4.0 40.0 68-69 30.034745775898777 34.0 28.5 37.5 2.0 40.0 70-71 29.387377655137556 34.0 28.0 37.0 2.0 39.0 72-73 28.80361660301771 34.0 26.5 36.0 2.0 39.0 74-75 28.15064408908748 33.5 26.0 36.0 2.0 39.0 76-77 27.379816875846103 33.0 25.5 35.0 2.0 37.5 78-79 26.568105247820696 32.0 23.5 35.0 2.0 37.0 80-81 25.888899760320303 31.5 21.0 35.0 2.0 36.5 82-83 25.19918830487559 31.0 19.0 34.5 2.0 36.0 84-85 24.501585290466046 31.0 16.5 34.0 2.0 35.5 86-87 23.7915111986484 30.5 8.5 34.0 2.0 35.0 88-89 22.966414900032866 30.0 3.5 34.0 2.0 35.0 90-91 22.08280667142285 29.0 2.0 34.0 2.0 35.0 92-93 21.18957141045113 29.0 2.0 34.0 2.0 35.0 94-95 20.185233316373903 27.5 2.0 33.5 2.0 35.0 96-97 18.895290904572775 25.5 2.0 33.0 2.0 34.5 98-99 17.375644761020524 21.5 2.0 32.0 2.0 34.0 100-101 14.004549811929476 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5908725802710926 1101 2 0.6209808902480134 1101 3 0.5809542278448312 1101 4 0.5675012132975752 1101 5 0.5540176331056514 1101 6 0.5462989227566979 1101 7 0.3917649961894796 1101 8 0.5944029915684794 1101 9 0.48957994712988295 1101 10-11 0.6455785859288525 1101 12-13 0.6383525894885764 1101 14-15 0.7298539789355587 1101 16-17 0.8366871287799071 1101 18-19 0.7838364511487512 1101 20-21 0.7393847170934293 1101 22-23 0.764148165415655 1101 24-25 0.9170984085354377 1101 26-27 0.878477539862395 1101 28-29 0.9285609286810228 1101 30-31 0.7762910960005911 1101 32-33 0.9189532341536193 1101 34-35 0.838699241563333 1101 36-37 0.8719263220894149 1101 38-39 0.7269080668376162 1101 40-41 0.7366125323861112 1101 42-43 0.7876600135498748 1101 44-45 0.9015863175411951 1101 46-47 0.9524851279224507 1101 48-49 0.9411803167114385 1101 50-51 0.744713017241196 1101 52-53 0.763127362119647 1101 54-55 0.8086290605019428 1101 56-57 0.6987624743913301 1101 58-59 0.7128625279302057 1101 60-61 0.6051819837682118 1101 62-63 0.7122860294103752 1101 64-65 0.589189832452 1101 66-67 0.47427382909076243 1101 68-69 0.42907177850305445 1101 70-71 0.7364282666945527 1101 72-73 0.5705452461022524 1101 74-75 0.5124810209531141 1101 76-77 0.7390921699870461 1101 78-79 0.7048364002398699 1101 80-81 0.7789798486332025 1101 82-83 0.6974833157791558 1101 84-85 0.9355734722092706 1101 86-87 1.25928265316686 1101 88-89 1.3316197138631516 1101 90-91 1.3883498961864937 1101 92-93 1.2069251651887107 1101 94-95 0.9326794652049628 1101 96-97 1.246957354184051 1101 98-99 1.3932774519515263 1101 100-101 1.0239579587510157 1102 1 0.3614573552068201 1102 2 0.4486999629744943 1102 3 0.3351254335618563 1102 4 0.3719905156636898 1102 5 0.44509785446606287 1102 6 0.3098681414590416 1102 7 0.34736218996698653 1102 8 0.38251794211098655 1102 9 0.3990171775102951 1102 10-11 0.5248897759589184 1102 12-13 0.4822079862378885 1102 14-15 0.5900085608345549 1102 16-17 0.6184041970617429 1102 18-19 0.5250119925666752 1102 20-21 0.5292039902643779 1102 22-23 0.4100161770937305 1102 24-25 0.39349226011938043 1102 26-27 0.36047185334010834 1102 28-29 0.28738048508870406 1102 30-31 0.2854085956737791 1102 32-33 0.45479520983882793 1102 34-35 0.47439687544716236 1102 36-37 0.23911389017516882 1102 38-39 0.5394494629768829 1102 40-41 0.5777537585833272 1102 42-43 0.5859061244915296 1102 44-45 0.6846601987560561 1102 46-47 0.6575681380754048 1102 48-49 0.4665038602209677 1102 50-51 0.6420119191541289 1102 52-53 0.579707605267501 1102 54-55 0.6407884138506716 1102 56-57 0.6489689104016136 1102 58-59 0.720416233974035 1102 60-61 0.7543818399710958 1102 62-63 0.4560689829159692 1102 64-65 0.5152759364216699 1102 66-67 0.5429581944225852 1102 68-69 0.5895831297601895 1102 70-71 0.5332402192850765 1102 72-73 0.543739611869821 1102 74-75 0.6514560307574655 1102 76-77 0.5582814592806962 1102 78-79 0.4470348620464861 1102 80-81 0.6676917750787972 1102 82-83 1.01202316501006 1102 84-85 0.8981094214881082 1102 86-87 0.8544360367565602 1102 88-89 1.185682527057626 1102 90-91 1.1680069618538411 1102 92-93 0.9380120317188378 1102 94-95 1.105666850276208 1102 96-97 1.2102705704461982 1102 98-99 1.0463680005593048 1102 100-101 0.8311559848087953 1103 1 0.4099775282967535 1103 2 0.43172531485745935 1103 3 0.402819111639257 1103 4 0.45296996463216743 1103 5 0.3738234019586315 1103 6 0.37873526806790636 1103 7 0.309417481306113 1103 8 0.15486704514005822 1103 9 0.18076160535481023 1103 10-11 0.41261320844224514 1103 12-13 0.49176818349935303 1103 14-15 0.6243199073416505 1103 16-17 0.6088278993838685 1103 18-19 0.5369163686389555 1103 20-21 0.4606293894031026 1103 22-23 0.5780315498549555 1103 24-25 0.6034164940688882 1103 26-27 0.6908820898594072 1103 28-29 0.5096498701468946 1103 30-31 0.6366482754636777 1103 32-33 0.6623284274024996 1103 34-35 0.7117881061567175 1103 36-37 0.709978095708486 1103 38-39 0.6749543312618798 1103 40-41 0.7133018052578066 1103 42-43 0.7087158144330559 1103 44-45 0.67670920675085 1103 46-47 0.44739403402698485 1103 48-49 0.5715607501014333 1103 50-51 0.6751042341326396 1103 52-53 0.6616411607308237 1103 54-55 0.638382342530015 1103 56-57 0.6241337044365096 1103 58-59 0.7529861218043195 1103 60-61 0.7640328895823387 1103 62-63 0.7804196081662376 1103 64-65 0.5951794802906036 1103 66-67 0.724160121872437 1103 68-69 0.7966501670849233 1103 70-71 0.7386083490335267 1103 72-73 0.8395855998865116 1103 74-75 0.8062529616417216 1103 76-77 0.7031984846346688 1103 78-79 0.7476160452598108 1103 80-81 0.6704687832659637 1103 82-83 0.8259840033608334 1103 84-85 0.8618974940068895 1103 86-87 0.9813653423372344 1103 88-89 0.9447588231672803 1103 90-91 0.8788053229569108 1103 92-93 1.086759910698074 1103 94-95 0.9911682161978987 1103 96-97 0.9797927958164792 1103 98-99 0.8816105575145308 1103 100-101 0.8604244508886687 1104 1 0.3576122503577679 1104 2 0.39311342322663734 1104 3 0.3692846240966645 1104 4 0.36763062845122363 1104 5 0.4011781248408752 1104 6 0.45564011278305117 1104 7 0.3058384175787765 1104 8 0.20938846148739998 1104 9 0.3475022302588968 1104 10-11 0.33285428044978715 1104 12-13 0.4058756342818981 1104 14-15 0.45664746962868463 1104 16-17 0.5301428734571587 1104 18-19 0.5758488250166351 1104 20-21 0.5155917093242834 1104 22-23 0.5727202778268037 1104 24-25 0.5191791119099776 1104 26-27 0.6539192670357679 1104 28-29 0.5802282872563111 1104 30-31 0.5807925617172245 1104 32-33 0.4690452650607426 1104 34-35 0.3616751970326604 1104 36-37 0.42726570326893665 1104 38-39 0.4473818194958099 1104 40-41 0.5120660149457734 1104 42-43 0.4337728991477334 1104 44-45 0.5545223046971088 1104 46-47 0.5031612214910908 1104 48-49 0.5509874860144208 1104 50-51 0.18745888485224782 1104 52-53 0.1883071877586815 1104 54-55 0.5004903088180654 1104 56-57 0.6990425914792127 1104 58-59 0.4375928953760351 1104 60-61 0.4043776399941237 1104 62-63 0.5316947017083287 1104 64-65 0.5214851616451774 1104 66-67 0.48584970746169986 1104 68-69 0.6102947199336946 1104 70-71 0.6915943883066689 1104 72-73 0.6823532668764543 1104 74-75 0.5523079520136029 1104 76-77 0.65940145314147 1104 78-79 0.5615692712652773 1104 80-81 0.8077735553712593 1104 82-83 0.7209002570911842 1104 84-85 0.6175698333347377 1104 86-87 0.626765136582236 1104 88-89 0.7934260401672546 1104 90-91 0.7762704424836535 1104 92-93 0.7581044104717272 1104 94-95 0.8362307568147926 1104 96-97 0.8230519818666089 1104 98-99 0.5540337363174963 1104 100-101 0.5884788261502578 1105 1 0.3766968106583839 1105 2 0.49127218452143495 1105 3 0.510729136815371 1105 4 0.4956210411691373 1105 5 0.45280114849790465 1105 6 0.4508834974008593 1105 7 0.4188007459412191 1105 8 0.3591497641603354 1105 9 0.34610096665984 1105 10-11 0.47653274018241376 1105 12-13 0.5338838705213504 1105 14-15 0.706413892871339 1105 16-17 0.6937708758835157 1105 18-19 0.762717616325844 1105 20-21 0.5900757134522507 1105 22-23 0.6296811015295276 1105 24-25 0.5420528938052129 1105 26-27 0.6595723760093719 1105 28-29 0.6163298793596113 1105 30-31 0.6464499837860913 1105 32-33 0.6730288713598327 1105 34-35 0.5982573349099027 1105 36-37 0.7511054018643222 1105 38-39 0.5899376927892845 1105 40-41 0.5884041576757468 1105 42-43 0.5321527311930438 1105 44-45 0.5338893628904131 1105 46-47 0.5739349789651556 1105 48-49 0.4532375825174668 1105 50-51 0.432961941438478 1105 52-53 0.6081166040925083 1105 54-55 0.5733220429580825 1105 56-57 0.6973639308627 1105 58-59 0.6395838122731305 1105 60-61 0.8766276971081695 1105 62-63 0.6509694123961332 1105 64-65 0.6900546123262146 1105 66-67 0.6607725050258821 1105 68-69 0.6491851515583313 1105 70-71 0.7523460091639329 1105 72-73 0.6093320716567057 1105 74-75 0.6362231593178969 1105 76-77 0.6011990882260392 1105 78-79 0.8099050861054771 1105 80-81 0.7832665086795423 1105 82-83 0.9375817931045454 1105 84-85 0.9057240845892807 1105 86-87 0.7868521665730199 1105 88-89 0.9300101474943965 1105 90-91 0.8580205350477996 1105 92-93 0.9324465157348847 1105 94-95 1.0085111434571985 1105 96-97 1.1033335541178495 1105 98-99 0.9159437876019716 1105 100-101 0.6606138673905377 1106 1 0.2289427169594802 1106 2 0.2675384072597282 1106 3 -0.021368582719844653 1106 4 0.3091756672661248 1106 5 0.3428820745862069 1106 6 -0.09499515691164362 1106 7 0.11386875703152555 1106 8 0.17690492423522386 1106 9 0.2844180278856214 1106 10-11 0.015607915739501266 1106 12-13 0.07467897230100817 1106 14-15 0.3331878290625596 1106 16-17 0.12992003423639886 1106 18-19 -0.21103423008442235 1106 20-21 0.21393903564788275 1106 22-23 0.01707456266999685 1106 24-25 0.1505884436207694 1106 26-27 -0.10990320398067865 1106 28-29 -0.08207213648594802 1106 30-31 0.07071981245501746 1106 32-33 0.13015325029456903 1106 34-35 0.10599448507934284 1106 36-37 -0.21305972448148225 1106 38-39 -0.1035400357508891 1106 40-41 0.3035307996972776 1106 42-43 -0.055368793803602046 1106 44-45 0.03162133916156762 1106 46-47 -0.21778875856416846 1106 48-49 0.10628485062018456 1106 50-51 -0.24489762474397025 1106 52-53 0.08889172119639 1106 54-55 -0.13877075939899441 1106 56-57 -0.4288237947548126 1106 58-59 -0.6880706740101132 1106 60-61 -0.12173995773357404 1106 62-63 -0.11397041635177985 1106 64-65 0.0612256313866979 1106 66-67 -0.4483499465330034 1106 68-69 -0.524155350191311 1106 70-71 -0.3998278180199648 1106 72-73 -0.11483625381704243 1106 74-75 -0.05290523629969357 1106 76-77 -0.172093722820037 1106 78-79 -0.11905847543231829 1106 80-81 -0.23399535376585234 1106 82-83 -0.22887762141554546 1106 84-85 -0.1492439438790143 1106 86-87 0.07008073834012052 1106 88-89 -0.23977310171371968 1106 90-91 -0.2508195243532967 1106 92-93 -0.42447100600733734 1106 94-95 -0.5298570643202396 1106 96-97 -0.4621438071150408 1106 98-99 -0.680302568946761 1106 100-101 -0.8100311715914277 1107 1 0.4733646165367169 1107 2 0.38805391156631686 1107 3 0.3063830443113851 1107 4 0.3416394963981233 1107 5 0.4020777490902958 1107 6 0.3097351720420036 1107 7 0.2882527851300978 1107 8 0.2492955832535273 1107 9 0.40046984339713987 1107 10-11 0.4705632710550489 1107 12-13 0.3841430411752569 1107 14-15 0.45058112914383486 1107 16-17 0.592717105092909 1107 18-19 0.38316361769290097 1107 20-21 0.4472698351200606 1107 22-23 0.534978435774228 1107 24-25 0.5163010141059701 1107 26-27 0.5267061194589004 1107 28-29 0.5525909866733443 1107 30-31 0.4944535153422578 1107 32-33 0.5809406963769277 1107 34-35 0.6340741496740918 1107 36-37 0.6671654383190955 1107 38-39 0.7573448470385458 1107 40-41 0.7089640802093555 1107 42-43 0.6048657637744981 1107 44-45 0.7048565535944462 1107 46-47 0.7888814360168794 1107 48-49 0.831529904230841 1107 50-51 0.799825122117447 1107 52-53 0.7210665927003888 1107 54-55 0.5353606622335434 1107 56-57 0.5902239217490077 1107 58-59 0.7073042497304058 1107 60-61 0.760553420507577 1107 62-63 0.7364814880397965 1107 64-65 0.6572082919798952 1107 66-67 0.575291757703031 1107 68-69 0.6229519549304001 1107 70-71 0.7031776336797648 1107 72-73 0.7497997180454021 1107 74-75 0.7205846311793671 1107 76-77 0.8708960579230087 1107 78-79 0.8282691781537785 1107 80-81 0.6216123733411969 1107 82-83 0.7452800159343447 1107 84-85 0.6749112570659221 1107 86-87 0.639959366390741 1107 88-89 0.9694382632383025 1107 90-91 1.002558015111596 1107 92-93 0.708970211337526 1107 94-95 0.847791161684583 1107 96-97 1.1384810820285338 1107 98-99 1.1434101808578383 1107 100-101 0.6289209988555662 1108 1 0.29037158157517595 1108 2 0.27749841282184207 1108 3 0.21273793176863265 1108 4 0.30360902293458025 1108 5 0.34438723313206765 1108 6 0.2273443345330577 1108 7 0.3167389970808756 1108 8 0.2367732849044515 1108 9 0.2122945016784925 1108 10-11 0.3276885167629686 1108 12-13 0.3023964413184217 1108 14-15 0.41844594149684156 1108 16-17 0.49906972010693806 1108 18-19 0.3784420487981066 1108 20-21 0.3722383049704163 1108 22-23 0.4327201953270148 1108 24-25 0.2953348848721262 1108 26-27 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2316 68-69 -0.2260984570232374 2316 70-71 -0.7362581993044515 2316 72-73 -0.48101301760604187 2316 74-75 -0.16574409385903266 2316 76-77 -0.03658194794209635 2316 78-79 -0.24223773428861506 2316 80-81 -0.33096408246106535 2316 82-83 -0.2719033383457834 2316 84-85 -0.5252214059699654 2316 86-87 -0.4812248257611884 2316 88-89 -0.670779583431159 2316 90-91 -0.824714712161132 2316 92-93 -0.7108594213953303 2316 94-95 -0.9655100532295364 2316 96-97 -0.8838551838561202 2316 98-99 -1.092262211266167 2316 100-101 -1.0603821710927654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6277.0 3 2229.0 4 2266.0 5 2580.0 6 3003.0 7 3912.0 8 4903.0 9 6327.0 10 8161.0 11 10034.0 12 12476.0 13 14547.0 14 16761.0 15 19717.0 16 22368.0 17 25486.0 18 24967.0 19 24375.0 20 25843.0 21 28418.0 22 31565.0 23 35079.0 24 39675.0 25 44679.0 26 50378.0 27 57420.0 28 65263.0 29 75711.0 30 87873.0 31 103256.0 32 121841.0 33 145809.0 34 176520.0 35 216473.0 36 250809.0 37 240404.0 38 108003.0 39 5338.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48963192029939 18.26006330288793 14.319952123707502 23.93035265310518 2 15.624286487945065 19.30855610634231 40.32288956107097 24.744267844641655 3 19.56921488967848 23.03807333130799 29.296623650111048 28.096088128902487 4 13.162844611262786 15.866141490825699 36.18849340298565 34.78252049492586 5 14.25652334325057 36.69939071742858 34.20677674705196 14.837309192268897 6 33.62358200039916 35.084151667864305 16.941777737306005 14.350488594430526 7 29.36757224629187 31.85879278586607 20.154150579833537 18.61948438800852 8 26.580312909337987 35.132136720126375 19.249787385986767 19.03776298454887 9 26.120278123702782 16.81006830446432 19.742160081512512 37.32749349032039 10-11 25.30795754222675 26.140522559023577 27.166634943389873 21.384884955359805 12-13 26.104530671901355 23.224808563740424 27.496961619350163 23.173699145008058 14-15 22.704611378177727 24.941823292333503 25.506951606554466 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22.98007882067071 26.259211151860907 28.15299190286456 22.60771812460382 42-43 22.71720457619065 26.243437024320592 28.002626958870643 23.036731440618112 44-45 22.705217990586394 26.535405241827576 28.085422724645966 22.673954042940064 46-47 23.046260925171342 26.735878183705815 27.47027888882103 22.74758200230181 48-49 22.856678012140875 26.76901565533966 27.447881359429104 22.92642497309036 50-51 22.93471315427728 26.905895362792464 27.48218045662359 22.67721102630667 52-53 23.117150322864504 26.62812790461338 27.49089976227854 22.763822010243572 54-55 22.792023262262582 26.802698022556775 27.585408457186833 22.81987025799381 56-57 22.789508220531747 26.996410642493345 27.430491123985508 22.783590012989404 58-59 22.75532236223306 26.68194757818931 27.524928549045146 23.03780151053249 60-61 22.55878433964395 26.835835345608743 27.66581789946978 22.939562415277525 62-63 22.61126253147605 26.757872549112065 27.822918336744223 22.80794658266766 64-65 22.467099640079553 26.837050232067934 27.647832048127718 23.048018079724798 66-67 22.462124855940534 26.830804865673386 27.54155325814891 23.16551702023717 68-69 22.78235794124894 26.5520977384485 27.716225568040482 22.949318752262084 70-71 22.681254020687796 26.596177681857714 27.42788230255947 23.294685994895023 72-73 22.618934766686333 26.725180784429686 27.40858436583138 23.2473000830526 74-75 22.666151338928522 26.949699186472316 27.24073830838403 23.143411166215124 76-77 22.797286297841357 26.717945501481253 27.314998646544346 23.169769554133037 78-79 22.846914827248153 26.82066241229665 27.321925472604295 23.0104972878509 80-81 22.9089649588455 26.848545451501728 27.102364347180863 23.140125242471907 82-83 22.8639892069784 26.926768678828623 27.17702455803807 23.032217556154905 84-85 22.8969610346313 26.92503841411989 27.136224709574634 23.041775841674177 86-87 23.007565883167373 26.84641219444565 26.996466047839924 23.149555874547055 88-89 22.992750994857445 26.904544153181398 26.806320109916598 23.29638474204456 90-91 22.938974353289844 26.883916343182857 26.902052977367884 23.27505632615942 92-93 23.075856458145417 26.51699356808923 27.076398837421813 23.33075113634354 94-95 22.87953598458942 26.415497933589986 27.203077965116773 23.50188811670382 96-97 23.145746242696177 26.196376243344755 27.075087115204333 23.58279039875474 98-99 22.97399207193271 26.2913293543648 26.87654900333205 23.85812957037044 100-101 23.70968716570219 25.713326025400264 26.995518934061487 23.58146787483607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 253.0 1 187.0 2 171.5 3 221.5 4 190.5 5 201.5 6 376.5 7 791.5 8 1275.0 9 1482.5 10 1343.0 11 1211.5 12 1248.5 13 1328.0 14 1421.5 15 1556.0 16 1710.0 17 1855.5 18 2095.5 19 2418.5 20 2774.5 21 3330.0 22 4159.5 23 5106.5 24 6463.5 25 8368.0 26 10872.5 27 13739.0 28 16359.5 29 19252.5 30 22625.0 31 27045.0 32 32758.5 33 35673.0 34 37047.0 35 40066.5 36 44485.0 37 49173.0 38 52664.5 39 55713.0 40 58908.0 41 62819.0 42 65728.0 43 67993.5 44 71116.5 45 75023.5 46 77508.5 47 79388.0 48 80513.5 49 80252.5 50 79596.0 51 77850.5 52 76692.0 53 76817.0 54 77080.0 55 73108.5 56 66989.0 57 61230.5 58 55887.5 59 49747.5 60 41060.0 61 34179.5 62 29078.5 63 22906.0 64 16859.5 65 12680.5 66 9716.0 67 7483.5 68 5772.0 69 4457.5 70 3533.0 71 2627.0 72 1922.0 73 1493.0 74 1124.0 75 845.5 76 634.0 77 431.0 78 280.5 79 182.0 80 118.0 81 72.5 82 45.5 83 30.5 84 16.5 85 14.0 86 10.0 87 7.0 88 6.0 89 6.0 90 6.0 91 3.0 92 0.5 93 0.5 94 1.5 95 3.0 96 2.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17088318408560757 2 0.0448426898635245 3 0.044465464291591594 4 0.0507368394249762 5 0.05681960177239435 6 0.06129915543909764 7 0.03498767179677727 8 0.033384463116062406 9 0.03899569349856442 10-11 0.04019810000910057 12-13 0.037038835844162454 14-15 0.032889354552900464 16-17 0.03069673091604043 18-19 0.038453431738910866 20-21 0.047318232679334216 22-23 0.04470123027404966 24-25 0.06511856435491833 26-27 0.04958158611093167 28-29 0.04248503003894383 30-31 0.05059537983550136 32-33 0.04715319649161357 34-35 0.04672881772318905 36-37 0.04441831109509998 38-39 0.02513265373003003 40-41 0.023152219477382264 42-43 0.02001653191068996 44-45 0.0044088238719658685 46-47 0.011434650149216291 48-49 0.010585892612367247 50-51 0.00858188176147367 52-53 0.009336332905339487 54-55 0.010892388389562734 56-57 0.00813392639480334 58-59 0.011151730970266609 60-61 0.010397279826400792 62-63 0.0041023280947703805 64-65 0.006577870910580093 66-67 0.004479553666703289 68-69 0.008935530735160771 70-71 0.02183192997561708 72-73 0.019049891382611883 74-75 0.01949784674928221 76-77 0.011741145926411779 78-79 0.004903932435127811 80-81 0.006625024107071707 82-83 0.005540500587764594 84-85 0.005068968622848459 86-87 0.008251809386032373 88-89 0.02800899871601846 90-91 0.034351103644140485 92-93 0.021666893787896435 94-95 0.03041381173709075 96-97 0.033455192910799826 98-99 0.020370180884377063 100-101 0.11793014442552555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2120747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.85203793881136 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62212729441539 49.24790838183048 2 12.068768516881237 14.929558563617407 3 3.7328678984013064 6.926564606174658 4 1.5576274252678564 3.853697224088019 5 0.8250192466902164 2.5514560873266414 6 0.48354085112873424 1.7944792241387766 7 0.32321996118473856 1.3994269311245708 8 0.2317461243267191 1.1467176059222977 9 0.16676924572316768 0.9283515942146688 >10 0.91051275237787 9.957750681125601 >50 0.0516219310226618 2.1569469033063395 >100 0.02250703509176353 2.551332141297847 >500 0.0025243057732320007 1.1281351926410614 >1k 0.001070917600765039 1.17426553204821 >5k 7.649411434035995E-5 0.2534093311434874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5356 0.25255252040908227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4046 0.1907818330050685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2755 0.12990705633439537 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.715319649161357E-5 0.0 0.0 0.0 0.0 9 9.430639298322714E-5 0.0 0.0 4.715319649161357E-5 0.0 10-11 9.430639298322714E-5 0.0 0.0 9.430639298322714E-5 0.0 12-13 1.1788299122903392E-4 0.0 0.0 9.430639298322714E-5 0.0 14-15 1.4145958947484071E-4 0.0 0.0 1.4145958947484071E-4 0.0 16-17 1.4145958947484071E-4 0.0 0.0 1.4145958947484071E-4 0.0 18-19 1.4145958947484071E-4 0.0 0.0 1.4145958947484071E-4 0.0 20-21 1.4145958947484071E-4 0.0 0.0 1.4145958947484071E-4 0.0 22-23 1.4145958947484071E-4 0.0 0.0 1.8861278596645428E-4 2.3576598245806785E-5 24-25 1.4145958947484071E-4 0.0 0.0 2.8291917894968143E-4 4.715319649161357E-5 26-27 1.4145958947484071E-4 0.0 0.0 4.715319649161357E-4 4.715319649161357E-5 28-29 1.4145958947484071E-4 0.0 0.0 0.002475542815809712 4.715319649161357E-5 30-31 1.4145958947484071E-4 0.0 0.0 0.007874583814099466 4.715319649161357E-5 32-33 1.4145958947484071E-4 0.0 0.0 0.02039375748262287 4.715319649161357E-5 34-35 1.4145958947484071E-4 0.0 0.0 0.0406460553757709 4.715319649161357E-5 36-37 1.4145958947484071E-4 0.0 0.0 0.07608168253921849 4.715319649161357E-5 38-39 1.6503618772064748E-4 0.0 0.0 0.12830384765368053 4.715319649161357E-5 40-41 1.8861278596645428E-4 0.0 0.0 0.18606651335590713 4.715319649161357E-5 42-43 2.1218938421226107E-4 0.0 0.0 0.24611610908797704 4.715319649161357E-5 44-45 2.3576598245806784E-4 0.0 0.0 0.3228107831815865 4.715319649161357E-5 46-47 3.3007237544129496E-4 0.0 0.0 0.4138871822051381 4.715319649161357E-5 48-49 3.3007237544129496E-4 0.0 0.0 0.5137576523743757 4.715319649161357E-5 50-51 3.3007237544129496E-4 0.0 0.0 0.6219978149208746 4.715319649161357E-5 52-53 3.3007237544129496E-4 0.0 0.0 0.7291770305463122 4.715319649161357E-5 54-55 3.3007237544129496E-4 0.0 0.0 0.8506436647087088 4.715319649161357E-5 56-57 3.3007237544129496E-4 0.0 0.0 0.9879537728922875 4.715319649161357E-5 58-59 3.3007237544129496E-4 0.0 0.0 1.1424747977953051 4.715319649161357E-5 60-61 3.3007237544129496E-4 0.0 0.0 1.3095621495633378 4.715319649161357E-5 62-63 3.3007237544129496E-4 0.0 0.0 1.4735845435594155 4.715319649161357E-5 64-65 3.3007237544129496E-4 0.0 0.0 1.645599404360822 4.715319649161357E-5 66-67 4.243787684245221E-4 0.0 0.0 1.8461655256379002 4.715319649161357E-5 68-69 4.243787684245221E-4 0.0 0.0 2.07410407747836 4.715319649161357E-5 70-71 4.4795536667032885E-4 0.0 0.0 2.3146796859785725 9.430639298322714E-5 72-73 4.715319649161357E-4 0.0 0.0 2.5607486418700582 9.430639298322714E-5 74-75 4.715319649161357E-4 0.0 0.0 2.809340293773845 9.430639298322714E-5 76-77 4.715319649161357E-4 0.0 0.0 3.0911042194094818 9.430639298322714E-5 78-79 4.715319649161357E-4 0.0 0.0 3.4051680846418737 9.430639298322714E-5 80-81 5.186851614077492E-4 0.0 0.0 3.73439170254632 1.1788299122903392E-4 82-83 5.186851614077492E-4 0.0 0.0 4.076110917521044 1.4145958947484071E-4 84-85 5.186851614077492E-4 0.0 0.0 4.4193154581852525 1.4145958947484071E-4 86-87 5.186851614077492E-4 0.0 0.0 4.782984486126822 1.4145958947484071E-4 88-89 5.186851614077492E-4 0.0 0.0 5.172092663575618 1.4145958947484071E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2700 0.0 55.006638 1 GTATCAA 4905 0.0 43.089176 1 ATCAACG 6175 0.0 34.017456 3 TCAACGC 6255 0.0 33.506405 4 TATCAAC 6445 0.0 32.739845 2 CAACGCA 6450 0.0 32.344532 5 AACGCAG 6735 0.0 31.18089 6 TGGTATC 1190 0.0 26.75743 2 ACGCAGA 7935 0.0 26.282093 7 CGCAGAG 7945 0.0 26.069635 8 GTGGTAT 1385 0.0 25.433506 1 GTACATG 5750 0.0 22.766127 1 TACATGG 5590 0.0 22.699482 2 GCAGAGT 8910 0.0 22.445873 9 ACATGGG 5675 0.0 21.522058 3 TAAGGTG 1120 0.0 21.215242 5 GAGTACT 5645 0.0 20.784252 12-13 GTGTAGC 1435 0.0 20.566673 1 CAGAGTA 8200 0.0 20.363325 10-11 GTACTTT 5760 0.0 20.205069 14-15 >>END_MODULE