FastQCFastQC Report
Thu 26 May 2016
SRR938630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.2463754516686809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.21435843125087528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.14683505387488466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.053.639171
GTATCAA53850.044.772981
ATCAACG68500.034.6991963
TCAACGC69100.034.4658854
TATCAAC69600.034.2995342
CAACGCA70400.033.694395
AACGCAG74200.032.1337056
TGGTATC14650.028.56532
CGCAGAG86800.027.1955368
ACGCAGA87550.027.0710667
GTGGTAT17350.025.764521
GCAGAGT98050.023.590789
TACATGG58700.023.3317932
GGACCGT2452.9831426E-1023.2635576
GTACATG60900.023.113631
ACATGGG60100.021.9892433
TAAGGTG12600.021.5047325
GAGTACT66550.020.83990512-13
TAGGTCG1150.00370763920.6681635
CAGAGTA92200.020.5543110-11
Produced by FastQC (version 0.11.5)