##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938630_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2120747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.619960796832437 31.0 31.0 34.0 30.0 34.0 2 31.866252315811362 31.0 31.0 34.0 30.0 34.0 3 31.929115071246123 31.0 31.0 34.0 30.0 34.0 4 35.6300534670095 37.0 35.0 37.0 33.0 37.0 5 35.48395353146792 37.0 35.0 37.0 33.0 37.0 6 35.51853002739129 37.0 35.0 37.0 33.0 37.0 7 35.45132163336787 37.0 35.0 37.0 33.0 37.0 8 35.440624459211776 37.0 35.0 37.0 33.0 37.0 9 36.99945538058052 39.0 37.0 39.0 33.0 39.0 10-11 36.89515604643081 39.0 37.0 39.0 33.0 39.0 12-13 36.769130876997586 39.0 37.0 39.0 32.0 39.0 14-15 37.77333529176276 39.5 37.0 41.0 32.0 41.0 16-17 37.674541093303446 39.0 37.0 41.0 32.0 41.0 18-19 37.77531289682362 39.5 37.0 41.0 32.0 41.0 20-21 37.835508903230796 40.0 37.0 41.0 32.0 41.0 22-23 37.72497285154712 39.5 37.0 41.0 32.0 41.0 24-25 37.60827788510369 39.0 37.0 41.0 32.0 41.0 26-27 37.38850037274602 39.0 36.0 41.0 32.0 41.0 28-29 37.25121431269265 39.0 36.0 41.0 31.0 41.0 30-31 36.998066011645896 39.0 36.0 40.0 31.0 41.0 32-33 36.76506745028992 39.0 35.5 40.0 30.0 41.0 34-35 36.59688496553337 38.5 35.0 40.0 30.0 41.0 36-37 36.58831770126281 39.0 35.0 40.0 30.0 41.0 38-39 36.45070109730204 39.0 35.0 40.0 30.0 41.0 40-41 36.25486562046298 38.5 35.0 40.0 30.0 41.0 42-43 36.020381261885554 38.0 35.0 40.0 29.5 41.0 44-45 35.74138546464995 38.0 35.0 40.0 28.5 41.0 46-47 35.36018841474254 38.0 34.0 40.0 27.0 41.0 48-49 34.9862291447306 38.0 34.0 40.0 26.0 41.0 50-51 34.57813921226813 38.0 33.0 40.0 25.0 41.0 52-53 34.02980695009825 37.0 33.0 40.0 24.0 41.0 54-55 33.665803134461584 37.0 32.0 40.0 23.0 41.0 56-57 33.284104846075465 37.0 32.0 39.0 22.0 40.5 58-59 32.77893237618632 36.0 31.0 39.0 20.5 40.0 60-61 32.353699427607346 36.0 31.0 39.0 19.5 40.0 62-63 31.844748572083326 35.0 30.0 38.5 17.5 40.0 64-65 31.287806607766036 35.0 29.5 38.0 14.0 40.0 66-67 30.741252256869867 34.0 29.0 38.0 11.5 40.0 68-69 30.145866291452965 34.0 28.0 37.0 8.0 39.0 70-71 29.504656142387564 34.0 27.5 36.5 7.0 39.0 72-73 29.002358131356544 33.0 26.5 36.0 4.5 39.0 74-75 28.422317466439893 33.0 26.0 35.5 2.0 38.0 76-77 27.254035016906776 31.5 25.5 34.0 2.0 36.0 78-79 27.93042899506636 32.5 26.0 35.0 2.0 37.0 80-81 27.600926701770646 32.5 26.0 35.0 2.0 36.5 82-83 27.055801564260143 32.0 25.0 35.0 2.0 36.0 84-85 26.445666550512627 32.0 24.5 34.5 2.0 35.5 86-87 25.77134707723269 31.0 22.5 34.0 2.0 35.0 88-89 25.01428647547303 31.0 20.0 34.0 2.0 35.0 90-91 24.35378501065898 31.0 17.5 34.0 2.0 35.0 92-93 23.4843408949771 30.0 8.5 34.0 2.0 35.0 94-95 22.562293616353106 29.5 2.0 34.0 2.0 35.0 96-97 21.394026491608855 29.0 2.0 34.0 2.0 35.0 98-99 19.984287376099083 28.0 2.0 33.5 2.0 35.0 100-101 16.484503573504995 20.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39401262016833627 1101 2 0.41683518445652723 1101 3 0.34572385355911806 1101 4 0.2158549730113748 1101 5 0.1805180499753618 1101 6 0.15867422489815652 1101 7 0.2828556416850958 1101 8 0.2939892466811571 1101 9 0.37045395833614947 1101 10-11 0.4903951585466757 1101 12-13 0.3900781878664432 1101 14-15 0.4063399151297702 1101 16-17 0.5678683178867772 1101 18-19 0.4394463765440051 1101 20-21 0.4659448271167932 1101 22-23 0.4430915015704713 1101 24-25 0.4992220556457099 1101 26-27 0.4996557023961614 1101 28-29 0.6300624938522503 1101 30-31 0.5925650732782515 1101 32-33 0.3891811780290553 1101 34-35 0.42362984800325165 1101 36-37 0.433655144182822 1101 38-39 0.6023575022202081 1101 40-41 0.7077309184492862 1101 42-43 0.8010866512463082 1101 44-45 0.5839307592629126 1101 46-47 0.656479612532685 1101 48-49 0.7333811326460946 1101 50-51 0.7869126335274785 1101 52-53 0.8327818460999126 1101 54-55 0.5183131378497237 1101 56-57 0.5776876433240616 1101 58-59 0.6600154735092829 1101 60-61 0.6953993391444939 1101 62-63 0.6618101361017779 1101 64-65 0.5318030000090275 1101 66-67 0.5132764438158226 1101 68-69 0.44211973183242037 1101 70-71 0.3068628598936911 1101 72-73 0.21893019488642196 1101 74-75 0.24559755659670657 1101 76-77 0.33301362905742593 1101 78-79 0.31677271969807563 1101 80-81 0.3253236944868334 1101 82-83 0.27345990242688245 1101 84-85 0.40270621847391297 1101 86-87 0.4012492632802953 1101 88-89 0.3500895564714419 1101 90-91 0.49927141540976905 1101 92-93 0.6106291721156829 1101 94-95 0.6958291118768507 1101 96-97 0.7819927977117693 1101 98-99 0.9121896143293604 1101 100-101 0.5623572115687487 1102 1 0.2779498287613045 1102 2 0.31730579319765084 1102 3 0.35487675782509953 1102 4 0.27439020330463393 1102 5 0.2175806670828635 1102 6 0.21524531732048047 1102 7 0.24182226332438006 1102 8 0.30686998222518724 1102 9 0.3170594749163911 1102 10-11 0.449974007298259 1102 12-13 0.3599254578999904 1102 14-15 0.4409220319976086 1102 16-17 0.38023813792251104 1102 18-19 0.3143722992421587 1102 20-21 0.4589330836857073 1102 22-23 0.440500430064084 1102 24-25 0.42631201599257196 1102 26-27 0.5879160841121447 1102 28-29 0.44124544300036206 1102 30-31 0.3913705767860236 1102 32-33 0.373297043644115 1102 34-35 0.41465342201519917 1102 36-37 0.4929605605408369 1102 38-39 0.498792532069011 1102 40-41 0.578639781580577 1102 42-43 0.4680868473332822 1102 44-45 0.4716181312616925 1102 46-47 0.391264352608502 1102 48-49 0.4764069725519775 1102 50-51 0.44580681192306315 1102 52-53 0.4708025659569799 1102 54-55 0.3466493398628856 1102 56-57 0.564678361873888 1102 58-59 0.6551525165176884 1102 60-61 0.8023108603436739 1102 62-63 0.47837458335438043 1102 64-65 0.8502547844005051 1102 66-67 0.7056530179584861 1102 68-69 0.6921611279716835 1102 70-71 0.6900484017473687 1102 72-73 0.6386609021067784 1102 74-75 0.3980807802665822 1102 76-77 0.4391560535639414 1102 78-79 0.43383664405207867 1102 80-81 0.44919238157559604 1102 82-83 0.4570752015684576 1102 84-85 0.5136958040767716 1102 86-87 0.3897890689363166 1102 88-89 0.5016871206354558 1102 90-91 0.43781721261225925 1102 92-93 0.17585478107467978 1102 94-95 0.2719173945906981 1102 96-97 0.36706404264618797 1102 98-99 0.15217338268528025 1102 100-101 0.12520744469437872 1103 1 0.23939706801035499 1103 2 0.3078786857420077 1103 3 0.32847751140822723 1103 4 0.20331899241740103 1103 5 0.24667579231586956 1103 6 0.14402104792915082 1103 7 0.28288062524292457 1103 8 0.26660555902260796 1103 9 0.38682822484451407 1103 10-11 0.3687860893016506 1103 12-13 0.3208615612019372 1103 14-15 0.4576849700935952 1103 16-17 0.426537185384241 1103 18-19 0.35372736256176296 1103 20-21 0.43174073493813836 1103 22-23 0.39779940747854425 1103 24-25 0.4308233735817737 1103 26-27 0.38605429050485185 1103 28-29 0.35745333492265985 1103 30-31 0.3761407328868813 1103 32-33 0.5063670898648596 1103 34-35 0.4026487842588793 1103 36-37 0.5637216815898327 1103 38-39 0.4763334966512289 1103 40-41 0.4078222045259636 1103 42-43 0.6561938670102947 1103 44-45 0.5775984950265567 1103 46-47 0.6094547912612143 1103 48-49 0.9303122121920424 1103 50-51 0.811977014234202 1103 52-53 0.7587616499896583 1103 54-55 0.8774647004965317 1103 56-57 1.0131869301182164 1103 58-59 0.7975787865425872 1103 60-61 0.8142238093649397 1103 62-63 0.7832348181486353 1103 64-65 0.8539888264309496 1103 66-67 0.9495581216859428 1103 68-69 0.7543749143191576 1103 70-71 0.5533555527368641 1103 72-73 0.6058353940288654 1103 74-75 0.8533107496235175 1103 76-77 0.7360019401689186 1103 78-79 0.7536920121295552 1103 80-81 0.7951304242458832 1103 82-83 0.9993128667153428 1103 84-85 0.7480814843245938 1103 86-87 0.7831193146140336 1103 88-89 0.7252726624615136 1103 90-91 0.7664732355794008 1103 92-93 0.8139036197014597 1103 94-95 0.974794664461907 1103 96-97 0.6787983188645121 1103 98-99 0.772777838318703 1103 100-101 0.6357870910497745 1104 1 0.2733767279027468 1104 2 0.2328758856282569 1104 3 0.25458296684292137 1104 4 0.12110846573786205 1104 5 0.10743258937602462 1104 6 0.14449447551028527 1104 7 0.16158051057546885 1104 8 0.19666735666920943 1104 9 0.27010871005391834 1104 10-11 0.3540506812483386 1104 12-13 0.31225520068510093 1104 14-15 0.4178663361547521 1104 16-17 0.4915377352432415 1104 18-19 0.332680569256091 1104 20-21 0.29776835229237975 1104 22-23 0.3994928918831846 1104 24-25 0.427586320118607 1104 26-27 0.45456722987623976 1104 28-29 0.5128154358759573 1104 30-31 0.4662894550084715 1104 32-33 0.508089134413396 1104 34-35 0.34270449788336776 1104 36-37 0.297317232956118 1104 38-39 0.2679280938444393 1104 40-41 0.46103524104249516 1104 42-43 0.6008863860617382 1104 44-45 0.44726676844693714 1104 46-47 0.46476239417479803 1104 48-49 0.36318855611958867 1104 50-51 0.45596048959919244 1104 52-53 0.5359768753718797 1104 54-55 0.5269817291520127 1104 56-57 0.4132295772227863 1104 58-59 0.3708668600094427 1104 60-61 0.42124964541996235 1104 62-63 0.4375945698522301 1104 64-65 0.4366470663983648 1104 66-67 0.38334574764278884 1104 68-69 0.3568514548543362 1104 70-71 0.13732583148590294 1104 72-73 -0.15652540576086693 1104 74-75 -0.062400236355994565 1104 76-77 0.051612874170999135 1104 78-79 -7.701292144979277E-4 1104 80-81 0.03712133663169581 1104 82-83 -0.04871340373844646 1104 84-85 0.07475423975645867 1104 86-87 0.16659565532983933 1104 88-89 0.03880216163448935 1104 90-91 -0.025187730916194084 1104 92-93 0.09092492069020963 1104 94-95 0.3176644771622428 1104 96-97 -0.031561268592849245 1104 98-99 0.21738284746303194 1104 100-101 0.11805317279927507 1105 1 0.3128805446966396 1105 2 0.3543889745922435 1105 3 0.2718989611393887 1105 4 0.24169906437185062 1105 5 0.24112711981978663 1105 6 0.15581093459692852 1105 7 0.20313866055301588 1105 8 0.16515773393702915 1105 9 0.2708842794218853 1105 10-11 0.2486126362030845 1105 12-13 0.38223310345730965 1105 14-15 0.6037497132098721 1105 16-17 0.4386519987871438 1105 18-19 0.42155494986409536 1105 20-21 0.3154441650843509 1105 22-23 0.40227720166976866 1105 24-25 0.4521846508128746 1105 26-27 0.44420634787779534 1105 28-29 0.4770047315219017 1105 30-31 0.5416754671875523 1105 32-33 0.43601583542726985 1105 34-35 0.5583575871624689 1105 36-37 0.5448724563311202 1105 38-39 0.5857404879865058 1105 40-41 0.5193082107366038 1105 42-43 0.7046574887173449 1105 44-45 0.6691881243738038 1105 46-47 0.4364448558296061 1105 48-49 0.40705706776692097 1105 50-51 0.4244705613659505 1105 52-53 0.36982256109494216 1105 54-55 0.454406318915602 1105 56-57 0.5067674182330322 1105 58-59 0.48745671813026803 1105 60-61 0.37565422163373086 1105 62-63 0.5212440983659263 1105 64-65 0.6147142876621174 1105 66-67 0.4503792243520728 1105 68-69 0.3248861998893524 1105 70-71 0.3803567360936242 1105 72-73 0.44378123758751187 1105 74-75 0.4154337035679063 1105 76-77 0.4844535496135336 1105 78-79 0.6720938054312775 1105 80-81 0.6052516984590284 1105 82-83 0.6121474231153954 1105 84-85 0.8490443515424353 1105 86-87 0.607488919023428 1105 88-89 0.6411314300232434 1105 90-91 0.8228314948536628 1105 92-93 0.7799191423242249 1105 94-95 1.029327953320081 1105 96-97 1.155201172422128 1105 98-99 0.58256366265773 1105 100-101 0.21305234563032016 1106 1 0.11638122006809226 1106 2 0.1412776844352912 1106 3 0.19473500780716435 1106 4 0.16592357206160102 1106 5 -0.03325655435890695 1106 6 0.02337098736279586 1106 7 0.09838854799110663 1106 8 0.12815369750646965 1106 9 0.022297425073041666 1106 10-11 0.135913283137846 1106 12-13 0.07812814836358228 1106 14-15 0.1878587363304831 1106 16-17 0.029570721189728033 1106 18-19 0.19565405963763283 1106 20-21 0.07931495191184723 1106 22-23 0.21207867800807634 1106 24-25 0.13164615069044316 1106 26-27 -0.05351397724839302 1106 28-29 0.10921422305803219 1106 30-31 0.2214840329513521 1106 32-33 0.2503517794069836 1106 34-35 5.788680775040689E-4 1106 36-37 -0.24845983037393182 1106 38-39 -0.07525552692695214 1106 40-41 0.23110163791102423 1106 42-43 0.31845718381280363 1106 44-45 0.33251407206608974 1106 46-47 -0.04722537332274612 1106 48-49 -0.13001628310237123 1106 50-51 -0.13402292584149222 1106 52-53 -0.21058900079040654 1106 54-55 -0.4626300247576296 1106 56-57 -0.38884069254368825 1106 58-59 -0.35048888879564544 1106 60-61 -0.6970784458070405 1106 62-63 -0.3941599988875595 1106 64-65 -0.914634946521943 1106 66-67 -0.7585928484458613 1106 68-69 -1.3185005904769262 1106 70-71 -1.19750687836801 1106 72-73 -0.9738666924582233 1106 74-75 -0.8218470969249978 1106 76-77 -0.8768254498888233 1106 78-79 -0.9817686478654046 1106 80-81 -1.4162867266221326 1106 82-83 -0.9106239498239326 1106 84-85 -0.8036218846242988 1106 86-87 -0.6581978666034054 1106 88-89 -0.5992867868381389 1106 90-91 -0.7417900009163816 1106 92-93 -1.0385122778516056 1106 94-95 -0.9599731359632315 1106 96-97 -0.9197971490230863 1106 98-99 -0.9992811875361944 1106 100-101 -1.0702742734838768 1107 1 0.13939569621178904 1107 2 0.20527755969135697 1107 3 0.14423320584205968 1107 4 0.20650881602370674 1107 5 0.1200630961241771 1107 6 0.19465805239141787 1107 7 0.10941571683007112 1107 8 0.1637678957050852 1107 9 0.14833928056109613 1107 10-11 0.21271607662231418 1107 12-13 0.2733073800579007 1107 14-15 0.3049003169920539 1107 16-17 0.28875167980788063 1107 18-19 0.23866021302199414 1107 20-21 0.24678387800301493 1107 22-23 0.2597020136998509 1107 24-25 0.2641663095050646 1107 26-27 0.3585148463968224 1107 28-29 0.30755269233301874 1107 30-31 0.31323504795765444 1107 32-33 0.38096627971982144 1107 34-35 0.2277669059138958 1107 36-37 0.3818541150233017 1107 38-39 0.3071776035025735 1107 40-41 0.1566088486951358 1107 42-43 0.18983634494883006 1107 44-45 0.36579896966885883 1107 46-47 0.30028627756576753 1107 48-49 0.3021755457801376 1107 50-51 0.32326299700048367 1107 52-53 0.3702981819653033 1107 54-55 0.4478330676055009 1107 56-57 0.5187519249543797 1107 58-59 0.31090169420682656 1107 60-61 0.12950760509237824 1107 62-63 0.39510773472956373 1107 64-65 0.4178072473340251 1107 66-67 0.47449918334045194 1107 68-69 0.5228270748039137 1107 70-71 0.4299352277773778 1107 72-73 0.43450235857179464 1107 74-75 0.6679542093778856 1107 76-77 0.41987448832394847 1107 78-79 0.7373763299243308 1107 80-81 0.7154476428654988 1107 82-83 0.5836585873897171 1107 84-85 0.5575457185964332 1107 86-87 0.6586167385722987 1107 88-89 0.5506153671387573 1107 90-91 0.5383845821172741 1107 92-93 0.2568586501847854 1107 94-95 0.09613132538661162 1107 96-97 0.31808280833007885 1107 98-99 0.09862369911250468 1107 100-101 -0.27243409775086036 1108 1 0.11928911862489855 1108 2 0.21427984349678653 1108 3 0.14758505958760892 1108 4 0.12085134138985154 1108 5 0.11718504678943731 1108 6 0.11510549646090595 1108 7 0.1362532712266855 1108 8 0.094448151582192 1108 9 0.17656416154188292 1108 10-11 0.14367641112595209 1108 12-13 0.12373630371516953 1108 14-15 0.22939782298562506 1108 16-17 0.29488386782983156 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0.5016135267303774 2316 88-89 0.5733496221310794 2316 90-91 0.827506213174388 2316 92-93 0.7251801136675411 2316 94-95 0.8642921967732917 2316 96-97 0.8106746251154426 2316 98-99 0.7337025987075307 2316 100-101 0.673949088954906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 48.0 5 76.0 6 184.0 7 515.0 8 1104.0 9 1932.0 10 3163.0 11 4890.0 12 7045.0 13 9723.0 14 12476.0 15 16185.0 16 19806.0 17 23843.0 18 23536.0 19 21843.0 20 22630.0 21 23971.0 22 26198.0 23 29181.0 24 33534.0 25 39047.0 26 46259.0 27 54915.0 28 65294.0 29 76770.0 30 90418.0 31 105644.0 32 125920.0 33 151590.0 34 187475.0 35 234505.0 36 283379.0 37 271511.0 38 103272.0 39 2851.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.65911033842448 19.495939858700197 15.251758938747296 23.593190864128022 2 16.243011146313528 20.44651297950188 39.00544578649725 24.305030087687353 3 19.726462114460585 23.59734619682845 29.940633649733815 26.735558038977143 4 13.88732366472757 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22.697945582382058 24-25 22.587620803741707 26.18362380929141 28.75130820865418 22.4774471783127 26-27 22.271273545578353 26.49749972648838 28.509720037273034 22.721506690660238 28-29 22.39027097527428 26.830428146308826 28.38792180302507 22.391379075391832 30-31 22.531848447740348 26.456951253496996 28.38455034947592 22.626649949286737 32-33 22.299501072027923 26.363823690426063 28.390397345840874 22.94627789170514 34-35 22.87009250794793 26.516791514158903 28.204961942126943 22.408154035766216 36-37 22.847390221976188 26.166671106929147 28.233114251568608 22.752824419526057 38-39 22.447422028990974 26.456645572604753 28.271835183386308 22.824097215017964 40-41 22.918037618404842 26.30006307065682 28.279915827198188 22.501983483740148 42-43 22.657377445346253 26.327775125398595 28.157680995408434 22.857166433846714 44-45 22.693371722322368 26.54967801439776 28.208456737177983 22.548493526101886 46-47 22.956722324728034 26.737795691801047 27.59310752296243 22.712374460508492 48-49 22.843531076549915 26.708207061002565 27.556964597851607 22.89129726459592 50-51 22.892381788115227 26.905118809551542 27.55175416963928 22.650745232693954 52-53 23.12906725790488 26.695310661762107 27.529073482126815 22.6465485982062 54-55 22.82061462305499 26.836534485254486 27.555102046590186 22.787748845100335 56-57 22.80599713214259 26.988815733324156 27.451624356889344 22.753562777643914 58-59 22.72626107687527 26.73234949760627 27.48571611795278 23.055673307565684 60-61 22.534677639529846 26.88531446702506 27.66091381951737 22.919094073927724 62-63 22.614861650163835 26.890029786674223 27.714668463517807 22.780440099644135 64-65 22.465032368311732 26.8406603899475 27.587001184016763 23.107306057724 66-67 22.44806251006426 26.91092499740068 27.45139506301201 23.18961742952305 68-69 22.75131676356726 26.638940364874358 27.572934791321856 23.036808080236522 70-71 22.562697277278847 26.711496803483303 27.35702436784037 23.36878155139748 72-73 22.586168930098687 26.792870625303255 27.336877053227 23.28408339137106 74-75 22.555142126807205 27.060158519615968 27.08458387539862 23.300115478178206 76-77 22.623584991514782 26.837666161970287 27.37264275276589 23.166106093749043 78-79 22.634736722485048 26.928436065216644 27.36971925458341 23.0671079577149 80-81 22.70847445079422 26.928830589023594 27.18461617897494 23.178078781207244 82-83 22.672411841926575 26.990158133779975 27.246036281743695 23.091393742549755 84-85 22.57339685536697 27.081233916918045 27.113535653808373 23.23183357390661 86-87 22.74137286730961 26.998301274456754 27.028267688967006 23.232058169266626 88-89 22.618354073298317 27.072638877526266 26.83538728969944 23.473619759475977 90-91 22.500620673118522 27.042651147756043 27.027632390813988 23.429095788311447 92-93 22.535761835008344 26.806528784997063 27.082966419841455 23.574742960153134 94-95 22.354067861026262 26.661561544852614 27.237938913948206 23.74643168017292 96-97 22.54472127038256 26.44662470346534 27.1586379704887 23.850016055663406 98-99 22.327617649187655 26.631155535782614 26.912283357228254 24.128943457801473 100-101 22.48048034029527 26.40409874541818 27.167485599999814 23.947935314286738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 246.0 1 186.5 2 175.5 3 236.0 4 219.0 5 205.0 6 350.0 7 813.5 8 1293.5 9 1486.5 10 1360.5 11 1124.5 12 1154.0 13 1289.5 14 1421.0 15 1632.5 16 1797.0 17 1981.5 18 2234.0 19 2486.5 20 2873.5 21 3533.5 22 4342.5 23 5275.5 24 6671.0 25 8572.0 26 11116.0 27 14186.0 28 16897.0 29 19638.5 30 23037.0 31 27558.5 32 33721.5 33 36836.0 34 37627.5 35 40147.0 36 44594.0 37 49528.5 38 53469.0 39 56315.0 40 59294.5 41 63627.0 42 66833.0 43 69106.5 44 71990.0 45 75736.5 46 77781.5 47 79798.0 48 81148.0 49 80682.0 50 80063.5 51 78033.0 52 76352.5 53 76417.0 54 77046.5 55 72914.5 56 66181.0 57 60441.5 58 55208.0 59 48749.0 60 39666.5 61 32664.5 62 27646.5 63 21527.0 64 15832.0 65 11877.0 66 8922.5 67 6878.5 68 5307.0 69 3990.5 70 2996.5 71 2218.0 72 1682.0 73 1332.5 74 1028.5 75 753.0 76 549.0 77 364.0 78 221.5 79 144.0 80 93.5 81 58.0 82 39.5 83 19.0 84 7.5 85 7.5 86 4.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7722557193290855E-4 2 0.044465464291591594 3 8.016043403574306E-4 4 0.0 5 0.08577166441824507 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.6503618772064748E-4 26-27 0.00844042217199883 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010680199005350473 36-37 7.072979473742036E-5 38-39 0.0041023280947703805 40-41 0.005163275015831686 42-43 4.4795536667032885E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3576598245806785E-5 68-69 8.016043403574306E-4 70-71 4.715319649161357E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001202406510536146 82-83 5.186851614077493E-4 84-85 0.005564077186010401 86-87 0.001862551261418736 88-89 1.1788299122903393E-4 90-91 0.0030885343702006887 92-93 7.308745456200103E-4 94-95 4.715319649161357E-5 96-97 0.0 98-99 1.6503618772064748E-4 100-101 2.3576598245806785E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2120747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.242111776912836 #Duplication Level Percentage of deduplicated Percentage of total 1 78.53318758150384 47.31005064482217 2 12.591092641603128 15.170280206178404 3 3.935274511681982 7.112077410167459 4 1.6750243312826811 4.036280119767198 5 0.8847338676145646 2.66491182728285 6 0.546318453656045 1.974682641056258 7 0.3437548307833678 1.449596185793378 8 0.2514471917337097 1.2118167864330378 9 0.18664335420693184 1.011941082589673 >10 0.9677884253984087 10.42471311432715 >50 0.05647496973137174 2.2970953469436948 >100 0.024258931754075303 2.6634654552545007 >500 0.0027457218969709706 1.1553991064245726 >1k 0.0011767379558445942 1.2707594311456258 >5k 7.844919705630627E-5 0.2469306418139721 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.2463754516686809 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4546 0.21435843125087528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3114 0.14683505387488466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.715319649161357E-5 0.0 5 0.0 0.0 0.0 4.715319649161357E-5 0.0 6 0.0 0.0 0.0 4.715319649161357E-5 0.0 7 0.0 0.0 0.0 4.715319649161357E-5 0.0 8 0.0 0.0 0.0 4.715319649161357E-5 0.0 9 0.0 0.0 0.0 4.715319649161357E-5 0.0 10-11 0.0 0.0 0.0 9.430639298322714E-5 0.0 12-13 0.0 0.0 0.0 9.430639298322714E-5 0.0 14-15 0.0 0.0 0.0 9.430639298322714E-5 0.0 16-17 0.0 0.0 0.0 9.430639298322714E-5 0.0 18-19 0.0 0.0 0.0 9.430639298322714E-5 0.0 20-21 0.0 0.0 0.0 9.430639298322714E-5 0.0 22-23 0.0 0.0 0.0 1.4145958947484071E-4 0.0 24-25 0.0 0.0 0.0 2.3576598245806784E-4 0.0 26-27 0.0 0.0 0.0 3.772255719329085E-4 0.0 28-29 2.3576598245806785E-5 0.0 0.0 0.0023812364228264853 0.0 30-31 4.715319649161357E-5 0.0 0.0 0.007733124224624625 0.0 32-33 4.715319649161357E-5 0.0 0.0 0.02022872129490222 0.0 34-35 4.715319649161357E-5 0.0 0.0 0.04048101918805025 0.0 36-37 4.715319649161357E-5 0.0 0.0 0.07584591655676043 0.0 38-39 4.715319649161357E-5 0.0 0.0 0.12804450507297666 0.0 40-41 4.715319649161357E-5 0.0 0.0 0.185594981390991 0.0 42-43 4.715319649161357E-5 0.0 0.0 0.24543238773884862 0.0 44-45 9.430639298322714E-5 0.0 0.0 0.32156122347455873 0.0 46-47 1.6503618772064748E-4 0.0 0.0 0.41226039692617744 0.0 48-49 1.8861278596645428E-4 0.0 0.0 0.5121072904971691 0.0 50-51 1.8861278596645428E-4 0.0 0.0 0.619640155096294 0.0 52-53 1.8861278596645428E-4 0.0 0.0 0.7261592259708489 0.0 54-55 1.8861278596645428E-4 0.0 0.0 0.8470128685788545 0.0 56-57 1.8861278596645428E-4 0.0 0.0 0.9838278681992714 0.0 58-59 1.8861278596645428E-4 0.0 0.0 1.137358675975965 0.0 60-61 1.8861278596645428E-4 0.0 0.0 1.3035265404124112 0.0 62-63 1.8861278596645428E-4 0.0 0.0 1.4657571129418079 0.0 64-65 1.8861278596645428E-4 0.0 0.0 1.637041099197594 0.0 66-67 1.8861278596645428E-4 0.0 0.0 1.835956858597466 0.0 68-69 1.8861278596645428E-4 0.0 0.0 2.0620092825782614 0.0 70-71 1.8861278596645428E-4 0.0 0.0 2.3011467185854797 0.0 72-73 2.1218938421226107E-4 0.0 0.0 2.5473571340664396 0.0 74-75 2.3576598245806784E-4 0.0 0.0 2.7964438945333887 0.0 76-77 2.3576598245806784E-4 0.0 0.0 3.080966282163785 0.0 78-79 2.3576598245806784E-4 0.0 0.0 3.398566637133048 0.0 80-81 3.7722557193290855E-4 0.0 0.0 3.7318925831322645 0.0 82-83 3.7722557193290855E-4 0.0 0.0 4.07792631558597 4.715319649161357E-5 84-85 3.7722557193290855E-4 0.0 0.0 4.425586833318637 4.715319649161357E-5 86-87 4.008021701787153E-4 0.0 0.0 4.79503212783043 4.715319649161357E-5 88-89 4.243787684245221E-4 0.0 0.0 5.192227078477536 4.715319649161357E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2970 0.0 53.63917 1 GTATCAA 5385 0.0 44.77298 1 ATCAACG 6850 0.0 34.699196 3 TCAACGC 6910 0.0 34.465885 4 TATCAAC 6960 0.0 34.299534 2 CAACGCA 7040 0.0 33.69439 5 AACGCAG 7420 0.0 32.133705 6 TGGTATC 1465 0.0 28.5653 2 CGCAGAG 8680 0.0 27.195536 8 ACGCAGA 8755 0.0 27.071066 7 GTGGTAT 1735 0.0 25.76452 1 GCAGAGT 9805 0.0 23.59078 9 TACATGG 5870 0.0 23.331793 2 GGACCGT 245 2.9831426E-10 23.263557 6 GTACATG 6090 0.0 23.11363 1 ACATGGG 6010 0.0 21.989243 3 TAAGGTG 1260 0.0 21.504732 5 GAGTACT 6655 0.0 20.839905 12-13 TAGGTCG 115 0.003707639 20.668163 5 CAGAGTA 9220 0.0 20.55431 10-11 >>END_MODULE