FastQCFastQC Report
Thu 26 May 2016
SRR938629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132216
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53870.2526479493634791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.19997973938850472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.1356804376292083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.055.735111
GTATCAA51300.045.7948721
ATCAACG64850.036.0544133
TCAACGC66150.035.3458634
TATCAAC67800.034.4147762
CAACGCA68500.034.2026445
AACGCAG70700.033.2673156
ACGCAGA81150.028.8601577
CGCAGAG83100.028.0685968
GCAGAGT95350.023.864659
GTACATG59700.022.9882281
TACATGG58650.022.8493712
GTAAGGT8600.022.1036934
ACATGGG59100.021.9522233
CAGAGTA87800.021.31627810-11
GAGTACT59550.021.2972712-13
AGAGTAC83650.020.95415310-11
GTGTAAG8600.020.5017341
TGGTATC12450.019.8484752
TAAGGTG11350.019.6791045
Produced by FastQC (version 0.11.5)