FastQCFastQC Report
Thu 26 May 2016
SRR938629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132216
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.24945877903551983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.21390890979150332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.14975030672314624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.052.95621
GTATCAA49050.041.599021
ATCAACG64600.031.1292553
TATCAAC66400.030.7293932
TCAACGC65900.030.587314
CAACGCA67900.029.6163445
AACGCAG70800.028.3759376
TGGTATC14550.026.151212
ACGCAGA82600.024.3222317
CGCAGAG83400.024.0319778
TACATGG56450.023.2546562
GTAAGGT10700.023.1036424
TAAGGTG12350.023.0964455
GTACATG59550.023.0025251
ACATGGG58750.022.0910153
GTGGTAT18350.022.0316811
GCAGAGT95050.020.836619
AGAGTAC85600.020.11302410-11
GAGTACT60000.019.86852512-13
CATGGGG41750.019.243814
Produced by FastQC (version 0.11.5)